Restriction map for
pFN21AB3761
(Circular) (Six-base) MAPSORT of: pFN21AB3761.seq Check: 7418 from: 1 to: 7909
pFN21AB3761 7909 bp
With 182 enzymes: SgfI *
February 27, 2009 14:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5719 278
Size: 53 83 186 936 4183 2468
Fragments arranged by size:
4183 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2399 5252 1937
Size: 462 2853 4594
Fragments arranged by size:
4594 2853 462
Acc65I G'GTAC_C
Cuts at: 5230 5230
Size: 7909
AclI AA'CG_TT
Cuts at: 1563 6036 6409 1563
Size: 4473 373 3063
Fragments arranged by size:
4473 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2751 5972 7356 7624 1632
Size: 1119 3221 1384 268 1917
Fragments arranged by size:
3221 1917 1384 1119 268
AfeI AGC'GCT
Cuts at: 5005 5005
Size: 7909
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7712
AflIII A'CryG_T
Cuts at: 1203 2334 3465 4469 5137 6808 7785 1203
Size: 1131 1131 1004 668 1671 977 1327
Fragments arranged by size:
1671 1327 1131 1131 1004 977 668
AgeI A'CCGG_T
Cuts at: 6740 6740
Size: 7909
AhdI GACnn_n'nnGTC
Cuts at: 3318 6638 3318
Size: 3320 4589
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7909
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7909
AlwNI CAG_nnn'CTG
Cuts at: 2074 2086 5652 7224 2074
Size: 12 3566 1572 2759
Fragments arranged by size:
3566 2759 1572 12
ApaLI G'TGCA_C
Cuts at: 5966 7122 5966
Size: 1156 6753
ApoI r'AATT_y
Cuts at: 1493 2354 2386 3399 4478 4799 5218 5444 5596
Size: 861 32 1013 1079 321 419 226 152
Cuts at: 5596 7705 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1079 1013 861 419 321 226
152 32
AseI AT'TA_AT
Cuts at: 160 1032 4277 6463 160
Size: 872 3245 2186 1606
Fragments arranged by size:
3245 2186 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7909
AvaI C'yCGr_G
Cuts at: 1870 1943 5234 1870
Size: 73 3291 4545
Fragments arranged by size:
4545 3291 73
BamHI G'GATC_C
Cuts at: 5239 5606 5239
Size: 367 7542
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5230 6685 618
Size: 325 217 627 3443 1455 1842
Fragments arranged by size:
3443 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3645 5228 1391
Size: 2254 1583 4072
Fragments arranged by size:
4072 2254 1583
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3454 3890 3985 928
Size: 971 1555 436 95 4852
Fragments arranged by size:
4852 1555 971 436 95
BbvCI CC'TCA_GC
Cuts at: 2028 2028
Size: 7909
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2114 2148 5099 5133 6100 6134 1903
Size: 34 177 34 2951 34 967 34 3678
Fragments arranged by size:
3678 2951 967 177 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5801 7011 5801
Size: 1210 6699
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7909
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2060 6520 136
Size: 107 122 71 631 605 388 4460 1525
Fragments arranged by size:
4460 1525 631 605 388 122 107 71
BglII A'GATC_T
Cuts at: 7904 7904
Size: 7909
BlpI GC'TnA_GC
Cuts at: 5326 5326
Size: 7909
Bme1580I G_kGCm'C
Cuts at: 1790 2654 5970 7126 1790
Size: 864 3316 1156 2573
Fragments arranged by size:
3316 2573 1156 864
BmgBI CAC'GTC
Cuts at: 1318 4470 1318
Size: 3152 4757
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4124 4368 5640
Size: 416 53 83 399 24 2707 244 1272
Cuts at: 5640 6598 442
Size: 958 1753
Fragments arranged by size:
2707 1753 1272 958 416 399 244 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7909
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2885 3661 2885
Size: 776 7133
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3722 4212 6569 1239
Size: 2483 490 2357 2579
Fragments arranged by size:
2579 2483 2357 490
Bpu10I CC'TnA_GC
Cuts at: 2028 4366 2028
Size: 2338 5571
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3631 6034 6899 7197 3631
Size: 2403 865 298 4343
Fragments arranged by size:
4343 2403 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6572 882
Size: 617 5073 2219
Fragments arranged by size:
5073 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4415 4636 5700 493
Size: 711 3211 221 1064 2702
Fragments arranged by size:
3211 2702 1064 711 221
BsaBI GATnn'nnATC
Cuts at: 7903 7903
Size: 7909
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5716 6098 6733 275
Size: 53 83 186 936 4183 382 635 1451
Fragments arranged by size:
4183 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3119 6342 6740 7014 7161 7572 3119
Size: 3223 398 274 147 411 3456
Fragments arranged by size:
3456 3223 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3801 3831 1178
Size: 30 165 30 2398 30 5256
Fragments arranged by size:
5256 2398 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2509 3840 4523 1186
Size: 1323 1331 683 4572
Fragments arranged by size:
4572 1331 1323 683
BseYI C'CCAG_C
Cuts at: 4944 7112 7608 4944
Size: 2168 496 5245
Fragments arranged by size:
5245 2168 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3705 3839 1163
Size: 573 1969 134 5233
Fragments arranged by size:
5233 1969 573 134
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2112 5359 6120 7148 1540
Size: 275 186 111 3247 761 1028 2301
Fragments arranged by size:
3247 2301 1028 761 275 186 111
BsiHKAI G_wGCw'C
Cuts at: 2811 3534 5970 6055 7126 2811
Size: 723 2436 85 1071 3594
Fragments arranged by size:
3594 2436 1071 723 85
BsmI GAATG_Cn'
Cuts at: 2870 5420 5513 2870
Size: 2550 93 5266
Fragments arranged by size:
5266 2550 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2654 2811 3534 3645 5228 5970 6055
Size: 399 864 157 723 111 1583 742 85
Cuts at: 6055 7126 1391
Size: 1071 2174
Fragments arranged by size:
2174 1583 1071 864 742 723 399 157
111 85
BspEI T'CCGG_A
Cuts at: 7572 7572
Size: 7909
BspHI T'CATG_A
Cuts at: 5798 7528 5798
Size: 1730 6179
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5264 844
Size: 4420 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3630 5796 1394
Size: 429 1807 2166 3507
Fragments arranged by size:
3507 2166 1807 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2748 6404 6578 65
Size: 1183 1500 3656 174 1396
Fragments arranged by size:
3656 1500 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3189 6553 6740 1700
Size: 110 1379 3364 187 2869
Fragments arranged by size:
3364 2869 1379 187 110
BsrGI T'GTAC_A
Cuts at: 96 5013 96
Size: 4917 2992
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7909
BssSI C'ACGA_G
Cuts at: 3015 5969 6981 3015
Size: 2954 1012 3943
Fragments arranged by size:
3943 2954 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3968 4187 794
Size: 3174 219 4516
Fragments arranged by size:
4516 3174 219
BstBI TT'CG_AA
Cuts at: 3880 3901 6713 3880
Size: 21 2812 5076
Fragments arranged by size:
5076 2812 21
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7909
BstYI r'GATC_y
Cuts at: 1972 2518 2623 2961 3356 5239 5606 5991 6008
Size: 546 105 338 395 1883 367 385 17
Cuts at: 6008 7460 7546 7558 7904 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1883 1452 546 395 385 367 346
338 105 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2223 513
Size: 552 1158 6199
Fragments arranged by size:
6199 1158 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7170
Fragments arranged by size:
7170 683 56
BtsI GCAGTG_nn'
Cuts at: 2515 2913 5425 6219 6239 2515
Size: 398 2512 794 20 4185
Fragments arranged by size:
4185 2512 794 398 20
ClaI AT'CG_AT
Cuts at: 1554 5109 1554
Size: 3555 4354
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7874
DraI TTT'AAA
Cuts at: 2686 5213 2686
Size: 2527 5382
DraIII CAC_nnn'GTG
Cuts at: 3230 3230
Size: 7909
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6916 809
Size: 507 5600 1802
Fragments arranged by size:
5600 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2109 4119 5356
Size: 54 1425 50 275 18 279 2010 1237
Cuts at: 5356 6245 7874 8
Size: 889 1629 43
Fragments arranged by size:
2010 1629 1425 1237 889 279 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2109 5356 1537
Size: 275 297 3247 4090
Fragments arranged by size:
4090 3247 297 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2727 2919 2979 3630 3898 4113 4819 5839
Size: 1374 192 60 651 268 215 706 1020
Cuts at: 5839 1353
Size: 3423
Fragments arranged by size:
3423 1374 1020 706 651 268 215 192
60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6496 6870 7016 387
Size: 1064 138 4907 374 146 1280
Fragments arranged by size:
4907 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2751 3722 4212 5972 6569 7356 7624
Size: 393 1119 971 490 1760 597 787 268
Cuts at: 7624 1239
Size: 1524
Fragments arranged by size:
1760 1524 1119 971 787 597 490 393
268
EcoNI CCTnn'n_nnAGG
Cuts at: 2078 2096 3960 2078
Size: 18 1864 6027
Fragments arranged by size:
6027 1864 18
EcoO109I rG'GnC_Cy
Cuts at: 2071 3994 4362 2071
Size: 1923 368 5618
Fragments arranged by size:
5618 1923 368
EcoRI G'AATT_C
Cuts at: 4478 5218 5596 4478
Size: 740 378 6791
Fragments arranged by size:
6791 740 378
EcoRV GAT'ATC
Cuts at: 5053 5053
Size: 7909
FspI TGC'GCA
Cuts at: 6415 6415
Size: 7909
HaeII r_GCGC'y
Cuts at: 1678 2066 5007 7056 1678
Size: 388 2941 2049 2531
Fragments arranged by size:
2941 2531 2049 388
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3304 3336 4867 4899 5108
Size: 32 135 32 1732 32 1531 32 209
Cuts at: 5108 5140 6550 6582 6624 6656 1373
Size: 32 1410 32 42 32 2626
Fragments arranged by size:
2626 1732 1531 1410 209 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3323 5253 5499 669
Size: 988 281 1385 1930 246 3079
Fragments arranged by size:
3079 1930 1385 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7909
HpaI GTT'AAC
Cuts at: 5499 5499
Size: 7909
KpnI G_GTAC'C
Cuts at: 5234 5234
Size: 7909
MfeI C'AATT_G
Cuts at: 5508 5508
Size: 7909
MluI A'CGCG_T
Cuts at: 5137 7785 5137
Size: 2648 5261
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2209 2516 7023 7207 1305
Size: 904 307 4507 184 2007
Fragments arranged by size:
4507 2007 904 307 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4121 10
Size: 54 1425 343 2289 3798
Fragments arranged by size:
3798 2289 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4977 5867 6226 6385 518
Size: 648 3811 890 359 159 2042
Fragments arranged by size:
3811 2042 890 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 5325 6002 7150 7395 1606
Size: 3719 677 1148 245 2120
Fragments arranged by size:
3719 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7357
NdeI CA'TA_TG
Cuts at: 387 2455 4872 387
Size: 2068 2417 3424
Fragments arranged by size:
3424 2417 2068
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7909
NotI GC'GGCC_GC
Cuts at: 5356 5356
Size: 7909
NspI r_CATG'y
Cuts at: 1132 1229 2187 2205 2338 3469 5267 6812 1132
Size: 97 958 18 133 1131 1798 1545 2229
Fragments arranged by size:
2229 1798 1545 1131 958 133 97 18
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7909
PciI A'CATG_T
Cuts at: 2334 3465 6808 2334
Size: 1131 3343 3435
Fragments arranged by size:
3435 3343 1131
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4172 4876 1108
Size: 3064 704 4141
Fragments arranged by size:
4141 3064 704
PfoI T'CCnGG_A
Cuts at: 2871 2871
Size: 7909
PmeI GTTT'AAAC
Cuts at: 5213 5213
Size: 7909
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5978 6010 7491 7523 5978
Size: 32 1481 32 6364
Fragments arranged by size:
6364 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3994 4362 3994
Size: 368 7541
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7909
PsiI TTA'TAA
Cuts at: 3242 3411 3606 4796 5479 3242
Size: 169 195 1190 683 5672
Fragments arranged by size:
5672 1190 683 195 169
PstI C_TGCA'G
Cuts at: 830 5261 830
Size: 4431 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7909
SalI G'TCGA_C
Cuts at: 1936 5251 1936
Size: 3315 4594
SapI GCTCTTCn'nnn_
Cuts at: 2727 2727
Size: 7909
SbfI CC_TGCA'GG
Cuts at: 5261 5261
Size: 7909
ScaI AGT'ACT
Cuts at: 6157 7869 6157
Size: 1712 6197
SexAI A'CCwGG_T
Cuts at: 4096 4096
Size: 7909
SfcI C'TryA_G
Cuts at: 826 1045 2066 2149 3541 3946 5177 5257 6392
Size: 219 1021 83 1392 405 1231 80 1135
Cuts at: 6392 7073 7264 826
Size: 681 191 1471
Fragments arranged by size:
1471 1392 1231 1135 1021 681 405 219
191 83 80
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7909
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7909
SmaI CCC'GGG
Cuts at: 1872 5236 1872
Size: 3364 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3646 6013 6914 7176 820
Size: 197 926 1703 2367 901 262 1553
Fragments arranged by size:
2367 1703 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5700 493
Size: 5207 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7909
SphI G_CATG'C
Cuts at: 1132 2205 5267 1132
Size: 1073 3062 3774
Fragments arranged by size:
3774 3062 1073
SspI AAT'ATT
Cuts at: 5 52 2409 3247 5833 5
Size: 47 2357 838 2586 2081
Fragments arranged by size:
2586 2357 2081 838 47
StuI AGG'CCT
Cuts at: 1517 4989 1517
Size: 3472 4437
StyI C'CwwG_G
Cuts at: 513 1065 3563 5348 513
Size: 552 2498 1785 3074
Fragments arranged by size:
3074 2498 1785 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3191 3876 5936 5953 6106 6291 6736
Size: 1255 1611 685 2060 17 153 185 445
Cuts at: 6736 325
Size: 1498
Fragments arranged by size:
2060 1611 1498 1255 685 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2755 5013 6155
Size: 274 80 33 51 1443 778 2258 1142
Cuts at: 6155 7867 96
Size: 1712 138
Fragments arranged by size:
2258 1712 1443 1142 778 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3801 3833 7869 7901 3801
Size: 32 4036 32 3809
Fragments arranged by size:
4036 3809 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7909
XbaI T'CTAG_A
Cuts at: 5245 5245
Size: 7909
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7909
XmaI C'CCGG_G
Cuts at: 1870 5234 1870
Size: 3364 4545
XmnI GAAnn'nnTTC
Cuts at: 6038 6038
Size: 7909
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5717 276
Size: 53 83 186 936 4183 2468
Fragments arranged by size:
4183 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI SalI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SfiI SfoI SrfI SwaI XcmI