Restriction map for
pFN21AB3755
(Circular) (Six-base) MAPSORT of: pFN21AB3755.seq Check: 9017 from: 1 to: 6475
pFN21AB3755 6475 bp
With 182 enzymes: SgfI *
August 16, 2010 14:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3163 3163
Size: 6475
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3333 4285 278
Size: 53 83 186 936 1797 952 2468
Fragments arranged by size:
2468 1797 952 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3818 1937
Size: 1881 4594
Acc65I G'GTAC_C
Cuts at: 2165 3796 2165
Size: 1631 4844
AclI AA'CG_TT
Cuts at: 1563 4602 4975 1563
Size: 3039 373 3063
Fragments arranged by size:
3063 3039 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2321 3431 4538 5922 6190 1632
Size: 689 1110 1107 1384 268 1917
Fragments arranged by size:
1917 1384 1110 1107 689 268
AflII C'TTAA_G
Cuts at: 820 1017 2326 820
Size: 197 1309 4969
Fragments arranged by size:
4969 1309 197
AflIII A'CryG_T
Cuts at: 1203 5374 6351 1203
Size: 4171 977 1327
Fragments arranged by size:
4171 1327 977
AgeI A'CCGG_T
Cuts at: 5306 5306
Size: 6475
AhdI GACnn_n'nnGTC
Cuts at: 3420 3737 5204 3420
Size: 317 1467 4691
Fragments arranged by size:
4691 1467 317
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6475
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6475
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2638 2670 3038 3070 2638
Size: 32 368 32 6043
Fragments arranged by size:
6043 368 32 32
AlwNI CAG_nnn'CTG
Cuts at: 4218 5790 4218
Size: 1572 4903
ApaLI G'TGCA_C
Cuts at: 4532 5688 4532
Size: 1156 5319
ApoI r'AATT_y
Cuts at: 1493 3784 4010 4162 6271 1493
Size: 2291 226 152 2109 1697
Fragments arranged by size:
2291 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5029 160
Size: 872 3997 1606
Fragments arranged by size:
3997 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6475
AvaI C'yCGr_G
Cuts at: 1870 1943 2095 2133 3761 3800 1870
Size: 73 152 38 1628 39 4545
Fragments arranged by size:
4545 1628 152 73 39 38
BamHI G'GATC_C
Cuts at: 3805 4172 3805
Size: 367 6108
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2165 3175 3796 5251 618
Size: 325 217 627 378 1010 621 1455 1842
Fragments arranged by size:
1842 1455 1010 627 621 378 325 217
BanII G_rGCy'C
Cuts at: 1391 2608 2691 3794 1391
Size: 1217 83 1103 4072
Fragments arranged by size:
4072 1217 1103 83
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2203 2309 3419 928
Size: 971 304 106 1110 3984
Fragments arranged by size:
3984 1110 971 304 106
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2695 2729 4666 4700 1903
Size: 34 758 34 1937 34 3678
Fragments arranged by size:
3678 1937 758 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2058 4367 5577 2058
Size: 2309 1210 2956
Fragments arranged by size:
2956 2309 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6475
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3392 5086 136
Size: 107 122 71 631 605 1720 1694 1525
Fragments arranged by size:
1720 1694 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6470 6470
Size: 6475
BlpI GC'TnA_GC
Cuts at: 3892 3892
Size: 6475
Bme1580I G_kGCm'C
Cuts at: 1790 4536 5692 1790
Size: 2746 1156 2573
Fragments arranged by size:
2746 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6475
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4206 5164 442
Size: 416 53 83 399 24 2789 958 1753
Fragments arranged by size:
2789 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6475
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3423 3423
Size: 6475
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2142 2779 3062 5135 1239
Size: 903 637 283 2073 2579
Fragments arranged by size:
2579 2073 903 637 283
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3095 4600 5465 5763 3095
Size: 1505 865 298 3807
Fragments arranged by size:
3807 1505 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5138 882
Size: 617 3639 2219
Fragments arranged by size:
3639 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2452 2529 4266 493
Size: 711 1248 77 1737 2702
Fragments arranged by size:
2702 1737 1248 711 77
BsaBI GATnn'nnATC
Cuts at: 6469 6469
Size: 6475
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3330 4282 4664 5299
Size: 53 83 186 936 1797 952 382 635
Cuts at: 5299 275
Size: 1451
Fragments arranged by size:
1797 1451 952 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4908 5306 5580 5727 6138 4908
Size: 398 274 147 411 5245
Fragments arranged by size:
5245 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2039 2060 2069 2090 2638
Size: 30 165 30 636 21 9 21 548
Cuts at: 2638 2668 3038 3062 3068 3092 3320 3350 1178
Size: 30 370 24 6 24 228 30 4303
Fragments arranged by size:
4303 636 548 370 228 165 30 30
30 30 24 24 21 21 9 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2047 2143 3046 3080 3197 3200 3328 1186
Size: 861 96 903 34 117 3 128 4333
Fragments arranged by size:
4333 903 861 128 117 96 34 3
BseYI C'CCAG_C
Cuts at: 2969 3190 5678 6174 2969
Size: 221 2488 496 3270
Fragments arranged by size:
3270 2488 496 221
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5902
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3925 4686 5714 1540
Size: 275 186 1924 761 1028 2301
Fragments arranged by size:
2301 1924 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3111 3356 4536 4621 5692 3111
Size: 245 1180 85 1071 3894
Fragments arranged by size:
3894 1180 1071 245 85
BsmI GAATG_Cn'
Cuts at: 3986 4079 3986
Size: 93 6382
BsmBI CGTCTCn'nnnn_
Cuts at: 3077 3077
Size: 6475
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2608 2691 3111 3356 3794 4536 4621
Size: 399 818 83 420 245 438 742 85
Cuts at: 4621 5692 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 818 742 438 420 399 245
85 83
BspEI T'CCGG_A
Cuts at: 6138 6138
Size: 6475
BspHI T'CATG_A
Cuts at: 2394 4364 6094 2394
Size: 1970 1730 2775
Fragments arranged by size:
2775 1970 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3163 3177 3830 844
Size: 2319 14 653 3489
Fragments arranged by size:
3489 2319 653 14
BsrBI CCG'CTC
Cuts at: 1394 1823 2524 4362 1394
Size: 429 701 1838 3507
Fragments arranged by size:
3507 1838 701 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2467 4970 5144 65
Size: 1183 1219 2503 174 1396
Fragments arranged by size:
2503 1396 1219 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2558 5119 5306 1700
Size: 110 748 2561 187 2869
Fragments arranged by size:
2869 2561 748 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6475
BssHII G'CGCG_C
Cuts at: 1925 3754 1925
Size: 1829 4646
BssSI C'ACGA_G
Cuts at: 2515 4535 5547 2515
Size: 2020 1012 3443
Fragments arranged by size:
3443 2020 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6475
BstBI TT'CG_AA
Cuts at: 5279 5279
Size: 6475
BstEII G'GTnAC_C
Cuts at: 1184 2245 1184
Size: 1061 5414
BstYI r'GATC_y
Cuts at: 1972 2763 3805 4172 4557 4574 6026 6112 6124
Size: 791 1042 367 385 17 1452 86 12
Cuts at: 6124 6470 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1042 791 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2410 3180 2410
Size: 770 5705
BtgI C'CryG_G
Cuts at: 513 1065 2853 3306 513
Size: 552 1788 453 3682
Fragments arranged by size:
3682 1788 552 453
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2200 2698 487
Size: 683 56 974 498 4264
Fragments arranged by size:
4264 974 683 498 56
BtsI GCAGTG_nn'
Cuts at: 3010 3991 4785 4805 3010
Size: 981 794 20 4680
Fragments arranged by size:
4680 981 794 20
ClaI AT'CG_AT
Cuts at: 1554 2401 1554
Size: 847 5628
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6440
DraI TTT'AAA
Cuts at: 3779 3779
Size: 6475
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5482 809
Size: 507 4166 1802
Fragments arranged by size:
4166 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2929 3357 3393
Size: 54 1425 50 275 18 1099 428 36
Cuts at: 3393 3922 4811 6440 8
Size: 529 889 1629 43
Fragments arranged by size:
1629 1425 1099 889 529 428 275 54
50 43 36 18
EagI C'GGCC_G
Cuts at: 1537 1812 3922 1537
Size: 275 2110 4090
Fragments arranged by size:
4090 2110 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3233 4405 1353
Size: 1880 1172 3423
Fragments arranged by size:
3423 1880 1172
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3040 5062 5436 5582 387
Size: 1064 138 1451 2022 374 146 1280
Fragments arranged by size:
2022 1451 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2142 2321 2779 3062 3431 4538 5135
Size: 393 510 179 458 283 369 1107 597
Cuts at: 5135 5922 6190 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1107 787 597 510 458 393 369
283 268 179
EcoNI CCTnn'n_nnAGG
Cuts at: 2184 3455 2184
Size: 1271 5204
EcoRI G'AATT_C
Cuts at: 3784 4162 3784
Size: 378 6097
FspI TGC'GCA
Cuts at: 4981 4981
Size: 6475
HaeII r_GCGC'y
Cuts at: 1678 5622 1678
Size: 3944 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2557 2589 3062 3094 3320
Size: 32 135 32 985 32 473 32 226
Cuts at: 3320 3352 3406 3438 3723 3755 5116 5148 5190
Size: 32 54 32 285 32 1361 32 42
Cuts at: 5190 5222 1373
Size: 32 2626
Fragments arranged by size:
2626 1361 985 473 285 226 135 54
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2290 2311 3334 3819 4065 669
Size: 988 281 352 21 1023 485 246 3079
Fragments arranged by size:
3079 1023 988 485 352 281 246 21
HindIII A'AGCT_T
Cuts at: 748 3113 3271 3281 748
Size: 2365 158 10 3942
Fragments arranged by size:
3942 2365 158 10
HpaI GTT'AAC
Cuts at: 4065 4065
Size: 6475
KpnI G_GTAC'C
Cuts at: 2169 3800 2169
Size: 1631 4844
MfeI C'AATT_G
Cuts at: 4074 4074
Size: 6475
MluI A'CGCG_T
Cuts at: 6351 6351
Size: 6475
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2617 2937 2993 3084 5589 5773 1305
Size: 1312 320 56 91 2505 184 2007
Fragments arranged by size:
2505 2007 1312 320 184 91 56
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3395 10
Size: 54 1425 343 1563 3090
Fragments arranged by size:
3090 1563 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2366 2393 2741 4433 4792 4951 518
Size: 648 1200 27 348 1692 359 159 2042
Fragments arranged by size:
2042 1692 1200 648 359 348 159 27
MspA1I CmG'CkG
Cuts at: 1606 2141 2299 2934 3561 3891 4568 5716 5961
Size: 535 158 635 627 330 677 1148 245
Cuts at: 5961 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 635 627 535 330 245
158
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5923
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6475
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6475
NotI GC'GGCC_GC
Cuts at: 3922 3922
Size: 6475
NspI r_CATG'y
Cuts at: 1132 1229 3833 5378 1132
Size: 97 2604 1545 2229
Fragments arranged by size:
2604 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6475
PciI A'CATG_T
Cuts at: 5374 5374
Size: 6475
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2961 1108
Size: 1853 4622
PfoI T'CCnGG_A
Cuts at: 2060 2656 2060
Size: 596 5879
PmeI GTTT'AAAC
Cuts at: 3779 3779
Size: 6475
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2092 2124 2638 2670 3038 3070 4544 4576 6057
Size: 32 514 32 368 32 1474 32 1481
Cuts at: 6057 6089 2092
Size: 32 2478
Fragments arranged by size:
2478 1481 1474 514 368 32 32 32
32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6475
PsiI TTA'TAA
Cuts at: 4045 4045
Size: 6475
PstI C_TGCA'G
Cuts at: 830 2876 3174 3231 3827 830
Size: 2046 298 57 596 3478
Fragments arranged by size:
3478 2046 596 298 57
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6475
PvuII CAG'CTG
Cuts at: 2141 2299 3561 2141
Size: 158 1262 5055
Fragments arranged by size:
5055 1262 158
SalI G'TCGA_C
Cuts at: 1936 3817 1936
Size: 1881 4594
SapI GCTCTTCn'nnn_
Cuts at: 3233 3233
Size: 6475
SbfI CC_TGCA'GG
Cuts at: 3174 3827 3174
Size: 653 5822
ScaI AGT'ACT
Cuts at: 4723 6435 4723
Size: 1712 4763
SfcI C'TryA_G
Cuts at: 826 1045 2872 3170 3227 3823 4958 5639 5830
Size: 219 1827 298 57 596 1135 681 191
Cuts at: 5830 826
Size: 1471
Fragments arranged by size:
1827 1471 1135 681 596 298 219 191
57
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6475
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6475
SmaI CCC'GGG
Cuts at: 1872 2097 3802 1872
Size: 225 1705 4545
Fragments arranged by size:
4545 1705 225
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2133 2326 3110 4579 5480 5742
Size: 197 926 190 193 784 1469 901 262
Cuts at: 5742 820
Size: 1553
Fragments arranged by size:
1553 1469 926 901 784 262 197 193
190
SnaBI TAC'GTA
Cuts at: 493 2452 4266 493
Size: 1959 1814 2702
Fragments arranged by size:
2702 1959 1814
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6475
SphI G_CATG'C
Cuts at: 1132 3833 1132
Size: 2701 3774
SspI AAT'ATT
Cuts at: 5 52 4399 5
Size: 47 4347 2081
Fragments arranged by size:
4347 2081 47
StuI AGG'CCT
Cuts at: 1517 3770 1517
Size: 2253 4222
StyI C'CwwG_G
Cuts at: 513 1065 2241 3771 3914 513
Size: 552 1176 1530 143 3074
Fragments arranged by size:
3074 1530 1176 552 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2561 3727 4502 4519 4672 4857 5302
Size: 1255 981 1166 775 17 153 185 445
Cuts at: 5302 325
Size: 1498
Fragments arranged by size:
1498 1255 1166 981 775 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2024 4721 6433
Size: 274 80 33 51 1443 47 2697 1712
Cuts at: 6433 96
Size: 138
Fragments arranged by size:
2697 1712 1443 274 138 80 51 47
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2060 2092 6435 6467 2060
Size: 32 4343 32 2068
Fragments arranged by size:
4343 2068 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6475
XbaI T'CTAG_A
Cuts at: 3811 3811
Size: 6475
XhoI C'TCGA_G
Cuts at: 1943 2133 1943
Size: 190 6285
XmaI C'CCGG_G
Cuts at: 1870 2095 3800 1870
Size: 225 1705 4545
Fragments arranged by size:
4545 1705 225
XmnI GAAnn'nnTTC
Cuts at: 4604 4604
Size: 6475
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3331 4283 276
Size: 53 83 186 936 1797 952 2468
Fragments arranged by size:
2468 1797 952 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BstXI BstZ17I DraIII EcoICRI EcoO109I EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI
XcmI