Restriction map for
pFN21AB3754
(Circular) (Six-base) MAPSORT of: pFN21AB3754.seq Check: 1815 from: 1 to: 6754
pFN21AB3754 6754 bp
With 182 enzymes: SgfI *
October 20, 2009 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2896 2896
Size: 6754
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4564 278
Size: 53 83 186 936 3028 2468
Fragments arranged by size:
3028 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4097 1937
Size: 2160 4594
Acc65I G'GTAC_C
Cuts at: 4075 4075
Size: 6754
AclI AA'CG_TT
Cuts at: 1563 4881 5254 1563
Size: 3318 373 3063
Fragments arranged by size:
3318 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3960 4817 6201 6469 1632
Size: 2328 857 1384 268 1917
Fragments arranged by size:
2328 1917 1384 857 268
AflII C'TTAA_G
Cuts at: 820 1017 3661 820
Size: 197 2644 3913
Fragments arranged by size:
3913 2644 197
AflIII A'CryG_T
Cuts at: 1203 2235 2871 5653 6630 1203
Size: 1032 636 2782 977 1327
Fragments arranged by size:
2782 1327 1032 977 636
AgeI A'CCGG_T
Cuts at: 5585 5585
Size: 6754
AhdI GACnn_n'nnGTC
Cuts at: 5483 5483
Size: 6754
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6754
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3213 3267 3861 1184
Size: 2029 54 594 4077
Fragments arranged by size:
4077 2029 594 54
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3366 3398 3366
Size: 32 6722
AlwNI CAG_nnn'CTG
Cuts at: 2592 2668 4018 4497 6069 2592
Size: 76 1350 479 1572 3277
Fragments arranged by size:
3277 1572 1350 479 76
ApaLI G'TGCA_C
Cuts at: 4811 5967 4811
Size: 1156 5598
ApoI r'AATT_y
Cuts at: 1493 2382 3730 3773 4063 4289 4441 6550 1493
Size: 889 1348 43 290 226 152 2109 1697
Fragments arranged by size:
2109 1697 1348 889 290 226 152 43
AseI AT'TA_AT
Cuts at: 160 1032 5308 160
Size: 872 4276 1606
Fragments arranged by size:
4276 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6754
AvaI C'yCGr_G
Cuts at: 1870 1943 4079 1870
Size: 73 2136 4545
Fragments arranged by size:
4545 2136 73
BamHI G'GATC_C
Cuts at: 4084 4451 4084
Size: 367 6387
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4075 5530 618
Size: 325 217 627 2288 1455 1842
Fragments arranged by size:
2288 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2077 3990 4073 1391
Size: 686 1913 83 4072
Fragments arranged by size:
4072 1913 686 83
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2056 2707 928
Size: 971 157 651 4975
Fragments arranged by size:
4975 971 651 157
BbvCI CC'TCA_GC
Cuts at: 3186 3186
Size: 6754
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4945 4979 1903
Size: 34 3008 34 3678
Fragments arranged by size:
3678 3008 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4646 5856 4646
Size: 1210 5544
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6754
BfrBI ATG'CAT
Cuts at: 2909 2909
Size: 6754
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5365 136
Size: 107 122 71 631 605 3693 1525
Fragments arranged by size:
3693 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2080 6749 2080
Size: 4669 2085
BlpI GC'TnA_GC
Cuts at: 3255 4171 3255
Size: 916 5838
Bme1580I G_kGCm'C
Cuts at: 1790 4815 5971 1790
Size: 3025 1156 2573
Fragments arranged by size:
3025 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2874 1318
Size: 1556 5198
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4485 5443 442
Size: 416 53 83 399 24 3068 958 1753
Fragments arranged by size:
3068 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6754
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3216 3216
Size: 6754
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2461 2839 3413 3974 3998 5414 1239
Size: 1222 378 574 561 24 1416 2579
Fragments arranged by size:
2579 1416 1222 574 561 378 24
Bpu10I CC'TnA_GC
Cuts at: 2126 2924 3186 2126
Size: 798 262 5694
Fragments arranged by size:
5694 798 262
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2061 3116 4879 5744 6042 2061
Size: 1055 1763 865 298 2773
Fragments arranged by size:
2773 1763 1055 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3473 5417 882
Size: 617 1974 1944 2219
Fragments arranged by size:
2219 1974 1944 617
BsaAI yAC'GTr
Cuts at: 493 1204 4545 493
Size: 711 3341 2702
Fragments arranged by size:
3341 2702 711
BsaBI GATnn'nnATC
Cuts at: 2085 3921 6748 2085
Size: 1836 2827 2091
Fragments arranged by size:
2827 2091 1836
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4561 4943 5578 275
Size: 53 83 186 936 3028 382 635 1451
Fragments arranged by size:
3028 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2365 2722 3555 5187 5585 5859 6006 6417 2365
Size: 357 833 1632 398 274 147 411 2702
Fragments arranged by size:
2702 1632 833 411 398 357 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3366 3396 1178
Size: 30 165 30 1963 30 4536
Fragments arranged by size:
4536 1963 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2324 3052 3374 3502 1186
Size: 1138 728 322 128 4438
Fragments arranged by size:
4438 1138 728 322 128
BseYI C'CCAG_C
Cuts at: 3518 5957 6453 3518
Size: 2439 496 3819
Fragments arranged by size:
3819 2439 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3953 1163
Size: 573 2217 3964
Fragments arranged by size:
3964 2217 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4204 4965 5993 1540
Size: 275 186 2203 761 1028 2301
Fragments arranged by size:
2301 2203 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2259 3202 3990 4815 4900 5971 2259
Size: 943 788 825 85 1071 3042
Fragments arranged by size:
3042 1071 943 825 788 85
BsmI GAATG_Cn'
Cuts at: 4265 4358 4265
Size: 93 6661
BsmBI CGTCTCn'nnnn_
Cuts at: 3180 3180
Size: 6754
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2077 2259 3202 3990 4073 4815 4900
Size: 399 287 182 943 788 83 742 85
Cuts at: 4900 5971 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 943 788 742 399 287 182
85 83
BspEI T'CCGG_A
Cuts at: 2365 6417 2365
Size: 4052 2702
BspHI T'CATG_A
Cuts at: 3471 4643 6373 3471
Size: 1172 1730 3852
Fragments arranged by size:
3852 1730 1172
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2896 4109 844
Size: 2052 1213 3489
Fragments arranged by size:
3489 2052 1213
BsrBI CCG'CTC
Cuts at: 1394 1823 3510 4641 1394
Size: 429 1687 1131 3507
Fragments arranged by size:
3507 1687 1131 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2186 5249 5423 65
Size: 1183 938 3063 174 1396
Fragments arranged by size:
3063 1396 1183 938 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2831 3108 5398 5585 1700
Size: 110 1021 277 2290 187 2869
Fragments arranged by size:
2869 2290 1021 277 187 110
BsrGI T'GTAC_A
Cuts at: 96 3625 96
Size: 3529 3225
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6754
BssSI C'ACGA_G
Cuts at: 4814 5826 4814
Size: 1012 5742
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6754
BstBI TT'CG_AA
Cuts at: 5558 5558
Size: 6754
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6754
BstXI CCAn_nnnn'nTGG
Cuts at: 3367 3367
Size: 6754
BstYI r'GATC_y
Cuts at: 1972 2080 2588 2650 3291 3796 3922 4084 4451
Size: 108 508 62 641 505 126 162 367
Cuts at: 4451 4836 4853 6305 6391 6403 6749 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 641 508 505 385 367 346
162 126 108 86 62 17 12
Bsu36I CC'TnA_GG
Cuts at: 3064 3064
Size: 6754
BtgI C'CryG_G
Cuts at: 513 1065 2037 2374 2538 513
Size: 552 972 337 164 4729
Fragments arranged by size:
4729 972 552 337 164
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6015
Fragments arranged by size:
6015 683 56
BtsI GCAGTG_nn'
Cuts at: 2669 4270 5064 5084 2669
Size: 1601 794 20 4339
Fragments arranged by size:
4339 1601 794 20
ClaI AT'CG_AT
Cuts at: 1554 3222 1554
Size: 1668 5086
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2973 3008 529
Size: 35 2409 35 4275
Fragments arranged by size:
4275 2409 35 35
DraI TTT'AAA
Cuts at: 4058 4058
Size: 6754
DraIII CAC_nnn'GTG
Cuts at: 2161 2421 3004 2161
Size: 260 583 5911
Fragments arranged by size:
5911 583 260
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5761 809
Size: 507 4445 1802
Fragments arranged by size:
4445 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4201 5090 6719
Size: 54 1425 50 275 18 2371 889 1629
Cuts at: 6719 8
Size: 43
Fragments arranged by size:
2371 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4201 1537
Size: 275 2389 4090
Fragments arranged by size:
4090 2389 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2688 3175 4684 1353
Size: 1335 487 1509 3423
Fragments arranged by size:
3423 1509 1335 487
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5341 5715 5861 387
Size: 1064 138 3752 374 146 1280
Fragments arranged by size:
3752 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3988 3988
Size: 6754
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2461 2839 3413 3960 3974 3998 4817
Size: 393 829 378 574 547 14 24 819
Cuts at: 4817 5414 6201 6469 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 829 819 787 597 574 547 393
378 268 24 14
EcoO109I rG'GnC_Cy
Cuts at: 2555 2555
Size: 6754
EcoRI G'AATT_C
Cuts at: 3730 4063 4441 3730
Size: 333 378 6043
Fragments arranged by size:
6043 378 333
FspI TGC'GCA
Cuts at: 5260 5260
Size: 6754
HaeII r_GCGC'y
Cuts at: 1678 5901 1678
Size: 4223 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2059 2091 2418 2450 2858
Size: 32 135 32 487 32 327 32 408
Cuts at: 2858 2890 5395 5427 5469 5501 1373
Size: 32 2505 32 42 32 2626
Fragments arranged by size:
2626 2505 487 408 327 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4098 4344 669
Size: 988 281 2160 246 3079
Fragments arranged by size:
3079 2160 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2123 3321 4029 748
Size: 1375 1198 708 3473
Fragments arranged by size:
3473 1375 1198 708
HpaI GTT'AAC
Cuts at: 4344 4344
Size: 6754
KpnI G_GTAC'C
Cuts at: 4079 4079
Size: 6754
MfeI C'AATT_G
Cuts at: 4353 4353
Size: 6754
MluI A'CGCG_T
Cuts at: 6630 6630
Size: 6754
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2119 3441 5868 6052 1305
Size: 814 1322 2427 184 2007
Fragments arranged by size:
2427 2007 1322 814 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4932
Fragments arranged by size:
4932 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2642 2832 4712 5071 5230 518
Size: 648 1476 190 1880 359 159 2042
Fragments arranged by size:
2042 1880 1476 648 359 190 159
MspA1I CmG'CkG
Cuts at: 1606 2056 3247 3976 4170 4847 5995 6240 1606
Size: 450 1191 729 194 677 1148 245 2120
Fragments arranged by size:
2120 1191 1148 729 677 450 245 194
NcoI C'CATG_G
Cuts at: 513 1065 2037 2374 513
Size: 552 972 337 4893
Fragments arranged by size:
4893 972 552 337
NdeI CA'TA_TG
Cuts at: 387 2168 387
Size: 1781 4973
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6754
NotI GC'GGCC_GC
Cuts at: 4201 4201
Size: 6754
NsiI A_TGCA'T
Cuts at: 2911 2911
Size: 6754
NspI r_CATG'y
Cuts at: 1132 1229 2121 2831 3094 4112 5657 1132
Size: 97 892 710 263 1018 1545 2229
Fragments arranged by size:
2229 1545 1018 892 710 263 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6754
PciI A'CATG_T
Cuts at: 5653 5653
Size: 6754
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6754
PfoI T'CCnGG_A
Cuts at: 3429 3429
Size: 6754
PmeI GTTT'AAAC
Cuts at: 4058 4058
Size: 6754
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3366 3398 4823 4855 6336 6368 3366
Size: 32 1425 32 1481 32 3752
Fragments arranged by size:
3752 1481 1425 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2555 2555
Size: 6754
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6754
PsiI TTA'TAA
Cuts at: 4324 4324
Size: 6754
PstI C_TGCA'G
Cuts at: 830 3688 4106 830
Size: 2858 418 3478
Fragments arranged by size:
3478 2858 418
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6754
PvuII CAG'CTG
Cuts at: 2056 3247 3976 2056
Size: 1191 729 4834
Fragments arranged by size:
4834 1191 729
SacI G_AGCT'C
Cuts at: 3990 3990
Size: 6754
SalI G'TCGA_C
Cuts at: 1936 4096 1936
Size: 2160 4594
SbfI CC_TGCA'GG
Cuts at: 4106 4106
Size: 6754
ScaI AGT'ACT
Cuts at: 5002 6714 5002
Size: 1712 5042
SexAI A'CCwGG_T
Cuts at: 2157 2157
Size: 6754
SfcI C'TryA_G
Cuts at: 826 1045 2107 3684 4102 5237 5918 6109 826
Size: 219 1062 1577 418 1135 681 191 1471
Fragments arranged by size:
1577 1471 1135 1062 681 418 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6754
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6754
SmaI CCC'GGG
Cuts at: 1872 4081 1872
Size: 2209 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2076 3095 3661 4858 5759 6021
Size: 197 926 133 1019 566 1197 901 262
Cuts at: 6021 820
Size: 1553
Fragments arranged by size:
1553 1197 1019 926 901 566 262 197
133
SnaBI TAC'GTA
Cuts at: 493 4545 493
Size: 4052 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6754
SphI G_CATG'C
Cuts at: 1132 2121 3094 4112 1132
Size: 989 973 1018 3774
Fragments arranged by size:
3774 1018 989 973
SspI AAT'ATT
Cuts at: 5 52 4678 5
Size: 47 4626 2081
Fragments arranged by size:
4626 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6754
StyI C'CwwG_G
Cuts at: 513 1065 2037 2374 4193 513
Size: 552 972 337 1819 3074
Fragments arranged by size:
3074 1819 972 552 337
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4781 4798 4951 5136 5581 325
Size: 1255 3201 17 153 185 445 1498
Fragments arranged by size:
3201 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2868 3625 5000
Size: 274 80 33 51 1443 891 757 1375
Cuts at: 5000 6712 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1375 891 757 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6714 6746 6714
Size: 32 6722
Tth111I GACn'n_nGTC
Cuts at: 1316 3965 1316
Size: 2649 4105
XbaI T'CTAG_A
Cuts at: 4090 4090
Size: 6754
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2485 2485
Size: 6754
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6754
XmaI C'CCGG_G
Cuts at: 1870 4079 1870
Size: 2209 4545
XmnI GAAnn'nnTTC
Cuts at: 4883 4883
Size: 6754
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4562 276
Size: 53 83 186 936 3028 2468
Fragments arranged by size:
3028 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacI SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BsiWI BstZ17I
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII
SacII SanDI SapI SfiI SfoI SrfI SwaI