Restriction map for
pFN21AB3747
(Circular) (Six-base) MAPSORT of: pFN21AB3747.seq Check: 979 from: 1 to: 5683
pFN21AB3747 5683 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3493 278
Size: 53 83 186 936 1957 2468
Fragments arranged by size:
2468 1957 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3026 1937
Size: 1089 4594
Acc65I G'GTAC_C
Cuts at: 3004 3004
Size: 5683
AclI AA'CG_TT
Cuts at: 1563 3810 4183 1563
Size: 2247 373 3063
Fragments arranged by size:
3063 2247 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3746 5130 5398 1632
Size: 2114 1384 268 1917
Fragments arranged by size:
2114 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5486
AflIII A'CryG_T
Cuts at: 1203 2376 4582 5559 1203
Size: 1173 2206 977 1327
Fragments arranged by size:
2206 1327 1173 977
AgeI A'CCGG_T
Cuts at: 4514 4514
Size: 5683
AhdI GACnn_n'nnGTC
Cuts at: 2473 4412 2473
Size: 1939 3744
AleI CACnn'nnGTG
Cuts at: 1166 2819 1166
Size: 1653 4030
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5683
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2619 2651 2619
Size: 32 5651
AlwNI CAG_nnn'CTG
Cuts at: 3426 4998 3426
Size: 1572 4111
ApaLI G'TGCA_C
Cuts at: 3740 4896 3740
Size: 1156 4527
ApoI r'AATT_y
Cuts at: 1493 2065 2992 3218 3370 5479 1493
Size: 572 927 226 152 2109 1697
Fragments arranged by size:
2109 1697 927 572 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4237 160
Size: 872 3205 1606
Fragments arranged by size:
3205 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5683
AvaI C'yCGr_G
Cuts at: 1870 1943 2296 3008 1870
Size: 73 353 712 4545
Fragments arranged by size:
4545 712 353 73
BamHI G'GATC_C
Cuts at: 2778 3013 3380 2778
Size: 235 367 5081
Fragments arranged by size:
5081 367 235
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2807 3004 4459 618
Size: 325 217 627 1020 197 1455 1842
Fragments arranged by size:
1842 1455 1020 627 325 217 197
BanII G_rGCy'C
Cuts at: 1391 2303 2343 2538 2697 3002 1391
Size: 912 40 195 159 305 4072
Fragments arranged by size:
4072 912 305 195 159 40
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2028 2043 2379 928
Size: 971 129 15 336 4232
Fragments arranged by size:
4232 971 336 129 15
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2574 2608 3874 3908 1903
Size: 34 637 34 1266 34 3678
Fragments arranged by size:
3678 1266 637 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3575 4785 3575
Size: 1210 4473
BclI T'GATC_A
Cuts at: 1548 2310 1548
Size: 762 4921
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2903 2922 4294
Size: 107 122 71 631 605 1231 19 1372
Cuts at: 4294 136
Size: 1525
Fragments arranged by size:
1525 1372 1231 631 605 122 107 71
19
BglII A'GATC_T
Cuts at: 5678 5678
Size: 5683
BlpI GC'TnA_GC
Cuts at: 3100 3100
Size: 5683
Bme1580I G_kGCm'C
Cuts at: 1790 3744 4900 1790
Size: 1954 1156 2573
Fragments arranged by size:
2573 1954 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5683
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3414 4372 442
Size: 416 53 83 399 24 1997 958 1753
Fragments arranged by size:
1997 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5683
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2246 4343 1239
Size: 1007 2097 2579
Fragments arranged by size:
2579 2097 1007
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2275 3808 4673 4971 2275
Size: 1533 865 298 2987
Fragments arranged by size:
2987 1533 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4346 882
Size: 617 2847 2219
Fragments arranged by size:
2847 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2209 3474 493
Size: 711 1005 1265 2702
Fragments arranged by size:
2702 1265 1005 711
BsaBI GATnn'nnATC
Cuts at: 5677 5677
Size: 5683
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2319 2676 2703 3490
Size: 53 83 186 936 786 357 27 787
Cuts at: 3490 3872 4507 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 787 786 635 382 357 186
83 53 27
BsaWI w'CCGG_w
Cuts at: 2460 4116 4514 4788 4935 5346 2460
Size: 1656 398 274 147 411 2797
Fragments arranged by size:
2797 1656 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2619 2649 2711 2741 1178
Size: 30 165 30 1216 30 62 30 4120
Fragments arranged by size:
4120 1216 165 62 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2579 2716 1186
Size: 1393 137 4153
Fragments arranged by size:
4153 1393 137
BseYI C'CCAG_C
Cuts at: 4886 5382 4886
Size: 496 5187
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5110
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2477 3133 3894 4922 1540
Size: 275 186 476 656 761 1028 2301
Fragments arranged by size:
2301 1028 761 656 476 275 186
BsiHKAI G_wGCw'C
Cuts at: 2303 3744 3829 4900 2303
Size: 1441 85 1071 3086
Fragments arranged by size:
3086 1441 1071 85
BsmI GAATG_Cn'
Cuts at: 3194 3287 3194
Size: 93 5590
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2303 2343 2538 2697 3002 3744 3829
Size: 399 513 40 195 159 305 742 85
Cuts at: 3829 4900 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 513 399 305 195 159
85 40
BspEI T'CCGG_A
Cuts at: 5346 5346
Size: 5683
BspHI T'CATG_A
Cuts at: 3572 5302 3572
Size: 1730 3953
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3038 844
Size: 2194 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2691 3570 1394
Size: 429 868 879 3507
Fragments arranged by size:
3507 879 868 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4178 4352 65
Size: 1183 2930 174 1396
Fragments arranged by size:
2930 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4327 4514 1700
Size: 110 2517 187 2869
Fragments arranged by size:
2869 2517 187 110
BsrGI T'GTAC_A
Cuts at: 96 2328 96
Size: 2232 3451
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5683
BssSI C'ACGA_G
Cuts at: 2417 3743 4755 2417
Size: 1326 1012 3345
Fragments arranged by size:
3345 1326 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5683
BstBI TT'CG_AA
Cuts at: 4487 4487
Size: 5683
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5683
BstYI r'GATC_y
Cuts at: 1972 2778 3013 3380 3765 3782 5234 5320 5332
Size: 806 235 367 385 17 1452 86 12
Cuts at: 5332 5678 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 806 385 367 346 235 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2345 2670 513
Size: 552 1280 325 3526
Fragments arranged by size:
3526 1280 552 325
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2915 487
Size: 683 56 1689 3255
Fragments arranged by size:
3255 1689 683 56
BtsI GCAGTG_nn'
Cuts at: 3199 3993 4013 3199
Size: 794 20 4869
Fragments arranged by size:
4869 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5683
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5648
DraI TTT'AAA
Cuts at: 2987 2987
Size: 5683
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4690 809
Size: 507 3374 1802
Fragments arranged by size:
3374 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2031 2904 3130
Size: 54 1425 50 275 18 201 873 226
Cuts at: 3130 4019 5648 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 873 275 226 201 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3130 1537
Size: 275 1318 4090
Fragments arranged by size:
4090 1318 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2481 3613 1353
Size: 1128 1132 3423
Fragments arranged by size:
3423 1132 1128
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2410 4270 4644 4790 387
Size: 1064 138 821 1860 374 146 1280
Fragments arranged by size:
1860 1280 1064 821 374 146 138
EcoICRI GAG'CTC
Cuts at: 2301 2301
Size: 5683
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2246 3746 4343 5130 5398 1239
Size: 393 614 1500 597 787 268 1524
Fragments arranged by size:
1524 1500 787 614 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2759 2759
Size: 5683
EcoO109I rG'GnC_Cy
Cuts at: 2413 2630 2413
Size: 217 5466
EcoRI G'AATT_C
Cuts at: 2992 3370 2992
Size: 378 5305
FspI TGC'GCA
Cuts at: 4189 4189
Size: 5683
HaeII r_GCGC'y
Cuts at: 1678 4830 1678
Size: 3152 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2366 2398 2459 2491 4324
Size: 32 135 32 794 32 61 32 1833
Cuts at: 4324 4356 4398 4430 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1833 794 135 61 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2077 2707 3027 3273 669
Size: 988 281 139 630 320 246 3079
Fragments arranged by size:
3079 988 630 320 281 246 139
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5683
HpaI GTT'AAC
Cuts at: 3273 3273
Size: 5683
KpnI G_GTAC'C
Cuts at: 3008 3008
Size: 5683
MfeI C'AATT_G
Cuts at: 3282 3282
Size: 5683
MluI A'CGCG_T
Cuts at: 5559 5559
Size: 5683
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2457 4797 4981 1305
Size: 1152 2340 184 2007
Fragments arranged by size:
2340 2007 1152 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2906 10
Size: 54 1425 343 1074 2787
Fragments arranged by size:
2787 1425 1074 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2279 2543 2819 3641 4000 4159 518
Size: 648 1113 264 276 822 359 159 2042
Fragments arranged by size:
2042 1113 822 648 359 276 264 159
MspA1I CmG'CkG
Cuts at: 1606 2337 2347 2599 3099 3776 4924 5169 1606
Size: 731 10 252 500 677 1148 245 2120
Fragments arranged by size:
2120 1148 731 677 500 252 245 10
NcoI C'CATG_G
Cuts at: 513 1065 2670 513
Size: 552 1605 3526
Fragments arranged by size:
3526 1605 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5683
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5683
NotI GC'GGCC_GC
Cuts at: 3130 3130
Size: 5683
NspI r_CATG'y
Cuts at: 1132 1229 2380 3041 4586 1132
Size: 97 1151 661 1545 2229
Fragments arranged by size:
2229 1545 1151 661 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5683
PciI A'CATG_T
Cuts at: 2376 4582 2376
Size: 2206 3477
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5683
PmeI GTTT'AAAC
Cuts at: 2987 2987
Size: 5683
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2619 2651 3752 3784 5265 5297 2619
Size: 32 1101 32 1481 32 3005
Fragments arranged by size:
3005 1481 1101 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2413 2413
Size: 5683
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5683
PsiI TTA'TAA
Cuts at: 3253 3253
Size: 5683
PstI C_TGCA'G
Cuts at: 830 2045 3035 830
Size: 1215 990 3478
Fragments arranged by size:
3478 1215 990
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5683
PvuII CAG'CTG
Cuts at: 2599 2599
Size: 5683
SacI G_AGCT'C
Cuts at: 2303 2303
Size: 5683
SacII CC_GC'GG
Cuts at: 2348 2348
Size: 5683
SalI G'TCGA_C
Cuts at: 1936 3025 1936
Size: 1089 4594
SbfI CC_TGCA'GG
Cuts at: 3035 3035
Size: 5683
ScaI AGT'ACT
Cuts at: 3931 5643 3931
Size: 1712 3971
SfcI C'TryA_G
Cuts at: 826 1045 2041 2430 3031 4166 4847 5038 826
Size: 219 996 389 601 1135 681 191 1471
Fragments arranged by size:
1471 1135 996 681 601 389 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5683
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5683
SmaI CCC'GGG
Cuts at: 1872 3010 1872
Size: 1138 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2254 3787 4688 4950 820
Size: 197 926 311 1533 901 262 1553
Fragments arranged by size:
1553 1533 926 901 311 262 197
SnaBI TAC'GTA
Cuts at: 493 3474 493
Size: 2981 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5683
SphI G_CATG'C
Cuts at: 1132 3041 1132
Size: 1909 3774
SspI AAT'ATT
Cuts at: 5 52 3607 5
Size: 47 3555 2081
Fragments arranged by size:
3555 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5683
StyI C'CwwG_G
Cuts at: 513 1065 2670 3122 513
Size: 552 1605 452 3074
Fragments arranged by size:
3074 1605 552 452
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3710 3727 3880 4065 4510 325
Size: 1255 2130 17 153 185 445 1498
Fragments arranged by size:
2130 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2328 3929 5641
Size: 274 80 33 51 1443 351 1601 1712
Cuts at: 5641 96
Size: 138
Fragments arranged by size:
1712 1601 1443 351 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5643 5675 5643
Size: 32 5651
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5683
XbaI T'CTAG_A
Cuts at: 3019 3019
Size: 5683
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5683
XmaI C'CCGG_G
Cuts at: 1870 3008 1870
Size: 1138 4545
XmnI GAAnn'nnTTC
Cuts at: 3812 3812
Size: 5683
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3491 276
Size: 53 83 186 936 1957 2468
Fragments arranged by size:
2468 1957 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SacII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI RsrII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI