Restriction map for
pFN21AB3746
(Circular) (Six-base) MAPSORT of: pFN21AB3746.seq Check: 1956 from: 1 to: 5731
pFN21AB3746 5731 bp
With 182 enzymes: SgfI *
October 20, 2009 10:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3541 278
Size: 53 83 186 936 2005 2468
Fragments arranged by size:
2468 2005 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3074 1937
Size: 1137 4594
Acc65I G'GTAC_C
Cuts at: 3052 3052
Size: 5731
AclI AA'CG_TT
Cuts at: 1563 3858 4231 1563
Size: 2295 373 3063
Fragments arranged by size:
3063 2295 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2550 2865 3794 5178 5446 1632
Size: 918 315 929 1384 268 1917
Fragments arranged by size:
1917 1384 929 918 315 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5534
AflIII A'CryG_T
Cuts at: 1203 4630 5607 1203
Size: 3427 977 1327
Fragments arranged by size:
3427 1327 977
AgeI A'CCGG_T
Cuts at: 4562 4562
Size: 5731
AhdI GACnn_n'nnGTC
Cuts at: 4460 4460
Size: 5731
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5731
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5731
AlwNI CAG_nnn'CTG
Cuts at: 2424 2950 3474 5046 2424
Size: 526 524 1572 3109
Fragments arranged by size:
3109 1572 526 524
ApaI G_GGCC'C
Cuts at: 2290 2290
Size: 5731
ApaLI G'TGCA_C
Cuts at: 3788 4944 3788
Size: 1156 4575
ApoI r'AATT_y
Cuts at: 1493 3040 3266 3418 5527 1493
Size: 1547 226 152 2109 1697
Fragments arranged by size:
2109 1697 1547 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4285 160
Size: 872 3253 1606
Fragments arranged by size:
3253 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5731
AvaI C'yCGr_G
Cuts at: 1870 1943 2133 2957 3056 1870
Size: 73 190 824 99 4545
Fragments arranged by size:
4545 824 190 99 73
BamHI G'GATC_C
Cuts at: 3061 3428 3061
Size: 367 5364
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3052 4507 618
Size: 325 217 627 1265 1455 1842
Fragments arranged by size:
1842 1455 1265 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2266 2290 3050 1391
Size: 875 24 760 4072
Fragments arranged by size:
4072 875 760 24
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2121 2332 2892 928
Size: 971 222 211 560 3767
Fragments arranged by size:
3767 971 560 222 211
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2168 2202 2212 2246 3922 3956 1903
Size: 34 231 34 10 34 1676 34 3678
Fragments arranged by size:
3678 1676 231 34 34 34 34 10
BciVI GTATCCnnnnn_n'
Cuts at: 2451 3623 4833 2451
Size: 1172 1210 3349
Fragments arranged by size:
3349 1210 1172
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5731
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4342 136
Size: 107 122 71 631 605 2670 1525
Fragments arranged by size:
2670 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5726 5726
Size: 5731
BlpI GC'TnA_GC
Cuts at: 3148 3148
Size: 5731
Bme1580I G_kGCm'C
Cuts at: 1790 2290 2834 3792 4948 1790
Size: 500 544 958 1156 2573
Fragments arranged by size:
2573 1156 958 544 500
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5731
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3462 4420 442
Size: 416 53 83 399 24 2045 958 1753
Fragments arranged by size:
2045 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5731
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2624 4391 1239
Size: 1385 1767 2579
Fragments arranged by size:
2579 1767 1385
Bpu10I CC'TnA_GC
Cuts at: 2082 2219 2082
Size: 137 5594
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3856 4721 5019 3856
Size: 865 298 4568
Fragments arranged by size:
4568 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4394 882
Size: 617 2895 2219
Fragments arranged by size:
2895 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3522 493
Size: 711 2318 2702
Fragments arranged by size:
2702 2318 711
BsaBI GATnn'nnATC
Cuts at: 2633 5725 2633
Size: 3092 2639
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3538 3920 4555 275
Size: 53 83 186 936 2005 382 635 1451
Fragments arranged by size:
2005 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4164 4562 4836 4983 5394 4164
Size: 398 274 147 411 4501
Fragments arranged by size:
4501 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5506
Fragments arranged by size:
5506 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2288 1186
Size: 1102 4629
BseYI C'CCAG_C
Cuts at: 2837 4934 5430 2837
Size: 2097 496 3138
Fragments arranged by size:
3138 2097 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2198 1163
Size: 573 462 4696
Fragments arranged by size:
4696 573 462
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2284 3181 3942 4970 1540
Size: 275 186 283 897 761 1028 2301
Fragments arranged by size:
2301 1028 897 761 283 275 186
BsiHKAI G_wGCw'C
Cuts at: 2105 2225 3792 3877 4948 2105
Size: 120 1567 85 1071 2888
Fragments arranged by size:
2888 1567 1071 120 85
BsmI GAATG_Cn'
Cuts at: 3242 3335 3242
Size: 93 5638
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2105 2225 2266 2290 2834 3050 3792
Size: 399 315 120 41 24 544 216 742
Cuts at: 3792 3877 4948 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 544 399 315 216 120
85 41 24
BspEI T'CCGG_A
Cuts at: 5394 5394
Size: 5731
BspHI T'CATG_A
Cuts at: 3620 5350 3620
Size: 1730 4001
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3086 844
Size: 2242 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3618 1394
Size: 429 1795 3507
Fragments arranged by size:
3507 1795 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4226 4400 65
Size: 1183 2978 174 1396
Fragments arranged by size:
2978 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4375 4562 1700
Size: 110 2565 187 2869
Fragments arranged by size:
2869 2565 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5731
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5731
BssSI C'ACGA_G
Cuts at: 3791 4803 3791
Size: 1012 4719
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5731
BstBI TT'CG_AA
Cuts at: 4535 4535
Size: 5731
BstEII G'GTnAC_C
Cuts at: 1184 2122 1184
Size: 938 4793
BstXI CCAn_nnnn'nTGG
Cuts at: 2454 2546 2454
Size: 92 5639
BstYI r'GATC_y
Cuts at: 1972 3061 3428 3813 3830 5282 5368 5380 5726
Size: 1089 367 385 17 1452 86 12 346
Cuts at: 5726 1972
Size: 1977
Fragments arranged by size:
1977 1452 1089 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2764 513
Size: 552 1699 3480
Fragments arranged by size:
3480 1699 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4992
Fragments arranged by size:
4992 683 56
BtsI GCAGTG_nn'
Cuts at: 2850 3247 4041 4061 2850
Size: 397 794 20 4520
Fragments arranged by size:
4520 794 397 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5731
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5696
DraI TTT'AAA
Cuts at: 2701 3035 2701
Size: 334 5397
DraIII CAC_nnn'GTG
Cuts at: 2984 2984
Size: 5731
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4738 809
Size: 507 3422 1802
Fragments arranged by size:
3422 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3178 4067 5696
Size: 54 1425 50 275 18 1348 889 1629
Cuts at: 5696 8
Size: 43
Fragments arranged by size:
1629 1425 1348 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3178 1537
Size: 275 1366 4090
Fragments arranged by size:
4090 1366 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2133 3661 1353
Size: 780 1528 3423
Fragments arranged by size:
3423 1528 780
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2432 4318 4692 4838 387
Size: 1064 138 843 1886 374 146 1280
Fragments arranged by size:
1886 1280 1064 843 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2550 2624 2865 3794 4391 5178 5446
Size: 393 918 74 241 929 597 787 268
Cuts at: 5446 1239
Size: 1524
Fragments arranged by size:
1524 929 918 787 597 393 268 241
74
EcoNI CCTnn'n_nnAGG
Cuts at: 2816 2816
Size: 5731
EcoO109I rG'GnC_Cy
Cuts at: 2182 2287 2962 2182
Size: 105 675 4951
Fragments arranged by size:
4951 675 105
EcoRI G'AATT_C
Cuts at: 3040 3418 3040
Size: 378 5353
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2393 2393
Size: 5731
FspI TGC'GCA
Cuts at: 4237 4237
Size: 5731
HaeII r_GCGC'y
Cuts at: 1678 4878 1678
Size: 3200 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2745 2765 2777 2797 4372
Size: 32 135 32 1173 20 12 20 1575
Cuts at: 4372 4404 4446 4478 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1575 1173 135 42 32 32 32
32 20 20 12
HincII GTy'rAC
Cuts at: 669 1657 1938 2305 3075 3321 669
Size: 988 281 367 770 246 3079
Fragments arranged by size:
3079 988 770 367 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5731
HpaI GTT'AAC
Cuts at: 3321 3321
Size: 5731
KpnI G_GTAC'C
Cuts at: 3056 3056
Size: 5731
MfeI C'AATT_G
Cuts at: 3330 3330
Size: 5731
MluI A'CGCG_T
Cuts at: 5607 5607
Size: 5731
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4845 5029 1305
Size: 3540 184 2007
Fragments arranged by size:
3540 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3909
Fragments arranged by size:
3909 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3689 4048 4207 518
Size: 648 2523 359 159 2042
Fragments arranged by size:
2523 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2316 3016 3147 3824 4972 5217 1606
Size: 710 700 131 677 1148 245 2120
Fragments arranged by size:
2120 1148 710 700 677 245 131
NcoI C'CATG_G
Cuts at: 513 1065 2764 513
Size: 552 1699 3480
Fragments arranged by size:
3480 1699 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5731
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5731
NotI GC'GGCC_GC
Cuts at: 3178 3178
Size: 5731
NspI r_CATG'y
Cuts at: 1132 1229 3089 4634 1132
Size: 97 1860 1545 2229
Fragments arranged by size:
2229 1860 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2418 1348
Size: 1070 4661
PciI A'CATG_T
Cuts at: 4630 4630
Size: 5731
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5731
PfoI T'CCnGG_A
Cuts at: 2640 2640
Size: 5731
PmeI GTTT'AAAC
Cuts at: 3035 3035
Size: 5731
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3800 3832 5313 5345 3800
Size: 32 1481 32 4186
Fragments arranged by size:
4186 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5731
PsiI TTA'TAA
Cuts at: 3301 3301
Size: 5731
PspOMI G'GGCC_C
Cuts at: 2286 2286
Size: 5731
PstI C_TGCA'G
Cuts at: 830 2148 2295 3083 830
Size: 1318 147 788 3478
Fragments arranged by size:
3478 1318 788 147
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5731
PvuII CAG'CTG
Cuts at: 2316 3016 2316
Size: 700 5031
SalI G'TCGA_C
Cuts at: 1936 3073 1936
Size: 1137 4594
SapI GCTCTTCn'nnn_
Cuts at: 2133 2133
Size: 5731
SbfI CC_TGCA'GG
Cuts at: 3083 3083
Size: 5731
ScaI AGT'ACT
Cuts at: 3979 5691 3979
Size: 1712 4019
SexAI A'CCwGG_T
Cuts at: 2118 2118
Size: 5731
SfcI C'TryA_G
Cuts at: 826 1045 2144 2291 2317 3079 4214 4895 5086
Size: 219 1099 147 26 762 1135 681 191
Cuts at: 5086 826
Size: 1471
Fragments arranged by size:
1471 1135 1099 762 681 219 191 147
26
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5731
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5731
SmaI CCC'GGG
Cuts at: 1872 2135 3058 1872
Size: 263 923 4545
Fragments arranged by size:
4545 923 263
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3835 4736 4998 820
Size: 197 926 1892 901 262 1553
Fragments arranged by size:
1892 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3522 493
Size: 3029 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5731
SphI G_CATG'C
Cuts at: 1132 3089 1132
Size: 1957 3774
SspI AAT'ATT
Cuts at: 5 52 2697 3655 5
Size: 47 2645 958 2081
Fragments arranged by size:
2645 2081 958 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5731
StyI C'CwwG_G
Cuts at: 513 1065 2764 2817 3170 513
Size: 552 1699 53 353 3074
Fragments arranged by size:
3074 1699 552 353 53
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3758 3775 3928 4113 4558 325
Size: 1255 2178 17 153 185 445 1498
Fragments arranged by size:
2178 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3018 3977 5689
Size: 274 80 33 51 1443 1041 959 1712
Cuts at: 5689 96
Size: 138
Fragments arranged by size:
1712 1443 1041 959 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2782 2814 5691 5723 2782
Size: 32 2877 32 2790
Fragments arranged by size:
2877 2790 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2120 1316
Size: 804 4927
XbaI T'CTAG_A
Cuts at: 3067 3067
Size: 5731
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2041 2041
Size: 5731
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5731
XmaI C'CCGG_G
Cuts at: 1870 2133 3056 1870
Size: 263 923 4545
Fragments arranged by size:
4545 923 263
XmnI GAAnn'nnTTC
Cuts at: 2903 3860 2903
Size: 957 4774
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3539 276
Size: 53 83 186 936 2005 2468
Fragments arranged by size:
2468 2005 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII
SanDI SfiI SfoI SrfI SwaI