Restriction map for
pFN21AB3738
(Circular) (Six-base) MAPSORT of: pFN21AB3738.seq Check: 3655 from: 1 to: 5776
pFN21AB3738 5776 bp
With 182 enzymes: SgfI *
October 20, 2009 10:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3586 278
Size: 53 83 186 936 2050 2468
Fragments arranged by size:
2468 2050 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3119 1937
Size: 1182 4594
Acc65I G'GTAC_C
Cuts at: 3097 3097
Size: 5776
AclI AA'CG_TT
Cuts at: 1563 3903 4276 1563
Size: 2340 373 3063
Fragments arranged by size:
3063 2340 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3839 5223 5491 1632
Size: 2207 1384 268 1917
Fragments arranged by size:
2207 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5579
AflIII A'CryG_T
Cuts at: 1203 2910 4675 5652 1203
Size: 1707 1765 977 1327
Fragments arranged by size:
1765 1707 1327 977
AgeI A'CCGG_T
Cuts at: 4607 4607
Size: 5776
AhdI GACnn_n'nnGTC
Cuts at: 2528 4505 2528
Size: 1977 3799
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5776
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5776
AlwNI CAG_nnn'CTG
Cuts at: 3519 5091 3519
Size: 1572 4204
ApaLI G'TGCA_C
Cuts at: 2045 2752 3833 4989 2045
Size: 707 1081 1156 2832
Fragments arranged by size:
2832 1156 1081 707
ApoI r'AATT_y
Cuts at: 1493 2179 2315 3085 3311 3463 5572 1493
Size: 686 136 770 226 152 2109 1697
Fragments arranged by size:
2109 1697 770 686 226 152 136
AseI AT'TA_AT
Cuts at: 160 1032 4330 160
Size: 872 3298 1606
Fragments arranged by size:
3298 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5776
AvaI C'yCGr_G
Cuts at: 1870 1943 2301 3101 1870
Size: 73 358 800 4545
Fragments arranged by size:
4545 800 358 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2832 2865 2832
Size: 33 5743
BamHI G'GATC_C
Cuts at: 2487 3106 3473 2487
Size: 619 367 4790
Fragments arranged by size:
4790 619 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2459 3097 4552 618
Size: 325 217 627 672 638 1455 1842
Fragments arranged by size:
1842 1455 672 638 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2690 2834 3095 1391
Size: 1299 144 261 4072
Fragments arranged by size:
4072 1299 261 144
BbeI G_GCGC'C
Cuts at: 2463 2463
Size: 5776
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4805
BbvCI CC'TCA_GC
Cuts at: 2114 2114
Size: 5776
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2276 2310 2914 2948 3967 4001 1903
Size: 34 339 34 604 34 1019 34 3678
Fragments arranged by size:
3678 1019 604 339 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3668 4878 3668
Size: 1210 4566
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5776
BfrBI ATG'CAT
Cuts at: 2122 2122
Size: 5776
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4387 136
Size: 107 122 71 631 605 337 2378 1525
Fragments arranged by size:
2378 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 2673 5771 2673
Size: 3098 2678
BlpI GC'TnA_GC
Cuts at: 3193 3193
Size: 5776
Bme1580I G_kGCm'C
Cuts at: 1790 2049 2756 3837 4993 1790
Size: 259 707 1081 1156 2573
Fragments arranged by size:
2573 1156 1081 707 259
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5776
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2167 3054 3507
Size: 416 53 83 399 24 750 887 453
Cuts at: 3507 4465 442
Size: 958 1753
Fragments arranged by size:
1753 958 887 750 453 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5776
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2090 2654 3008 3020 4436 1239
Size: 851 564 354 12 1416 2579
Fragments arranged by size:
2579 1416 851 564 354 12
Bpu10I CC'TnA_GC
Cuts at: 2114 2114
Size: 5776
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2583 3032 3901 4766 5064 2583
Size: 449 869 865 298 3295
Fragments arranged by size:
3295 869 865 449 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4439 882
Size: 617 2940 2219
Fragments arranged by size:
2940 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2913 3567 493
Size: 711 1709 654 2702
Fragments arranged by size:
2702 1709 711 654
BsaBI GATnn'nnATC
Cuts at: 5770 5770
Size: 5776
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2460 3583 3965 4600
Size: 53 83 186 936 927 1123 382 635
Cuts at: 4600 275
Size: 1451
Fragments arranged by size:
1451 1123 936 927 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4209 4607 4881 5028 5439 4209
Size: 398 274 147 411 4546
Fragments arranged by size:
4546 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5551
Fragments arranged by size:
5551 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5776
BseYI C'CCAG_C
Cuts at: 2206 2546 2722 2827 4979 5475 2206
Size: 340 176 105 2152 496 2507
Fragments arranged by size:
2507 2152 496 340 176 105
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5203
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3226 3987 5015 1540
Size: 275 186 1225 761 1028 2301
Fragments arranged by size:
2301 1225 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2049 2299 2756 3837 3922 4993 2049
Size: 250 457 1081 85 1071 2832
Fragments arranged by size:
2832 1081 1071 457 250 85
BsmI GAATG_Cn'
Cuts at: 2122 3287 3380 2122
Size: 1165 93 4518
Fragments arranged by size:
4518 1165 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2049 2299 2690 2756 2834 3095 3837
Size: 399 259 250 391 66 78 261 742
Cuts at: 3837 3922 4993 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 391 261 259 250
85 78 66
BspEI T'CCGG_A
Cuts at: 5439 5439
Size: 5776
BspHI T'CATG_A
Cuts at: 3665 5395 3665
Size: 1730 4046
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3131 844
Size: 2287 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3663 1394
Size: 429 1840 3507
Fragments arranged by size:
3507 1840 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2592 4271 4445 65
Size: 1183 1344 1679 174 1396
Fragments arranged by size:
1679 1396 1344 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2394 4420 4607 1700
Size: 110 584 2026 187 2869
Fragments arranged by size:
2869 2026 584 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5776
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5776
BssSI C'ACGA_G
Cuts at: 2573 2813 3836 4848 2573
Size: 240 1023 1012 3501
Fragments arranged by size:
3501 1023 1012 240
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5776
BstBI TT'CG_AA
Cuts at: 4580 4580
Size: 5776
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5776
BstXI CCAn_nnnn'nTGG
Cuts at: 2206 2587 2206
Size: 381 5395
BstYI r'GATC_y
Cuts at: 1972 2487 2673 3106 3473 3858 3875 5327 5413
Size: 515 186 433 367 385 17 1452 86
Cuts at: 5413 5425 5771 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 515 433 385 367 346 186
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2691 2691
Size: 5776
BtgI C'CryG_G
Cuts at: 513 1065 2004 2199 513
Size: 552 939 195 4090
Fragments arranged by size:
4090 939 552 195
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5037
Fragments arranged by size:
5037 683 56
BtsI GCAGTG_nn'
Cuts at: 3292 4086 4106 3292
Size: 794 20 4962
Fragments arranged by size:
4962 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5776
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5741
DraI TTT'AAA
Cuts at: 3019 3080 3019
Size: 61 5715
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2617 4783 809
Size: 507 1301 2166 1802
Fragments arranged by size:
2166 1802 1301 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2016 2577 2915
Size: 54 1425 50 275 18 186 561 338
Cuts at: 2915 3223 4112 5741 8
Size: 308 889 1629 43
Fragments arranged by size:
1629 1425 889 561 338 308 275 186
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3223 1537
Size: 275 1411 4090
Fragments arranged by size:
4090 1411 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3706 1353
Size: 2353 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2523 4363 4737 4883 387
Size: 1064 138 934 1840 374 146 1280
Fragments arranged by size:
1840 1280 1064 934 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2090 2654 3008 3020 3839 4436 5223
Size: 393 458 564 354 12 819 597 787
Cuts at: 5223 5491 1239
Size: 268 1524
Fragments arranged by size:
1524 819 787 597 564 458 393 354
268 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2091 2091
Size: 5776
EcoO109I rG'GnC_Cy
Cuts at: 2530 2808 2530
Size: 278 5498
EcoRI G'AATT_C
Cuts at: 2315 3085 3463 2315
Size: 770 378 4628
Fragments arranged by size:
4628 770 378
EcoRV GAT'ATC
Cuts at: 2884 2884
Size: 5776
FspI TGC'GCA
Cuts at: 4282 4282
Size: 5776
HaeII r_GCGC'y
Cuts at: 1678 2463 4923 1678
Size: 785 2460 2531
Fragments arranged by size:
2531 2460 785
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2514 2546 4417 4449 4491
Size: 32 135 32 942 32 1871 32 42
Cuts at: 4491 4523 1373
Size: 32 2626
Fragments arranged by size:
2626 1871 942 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3120 3366 669
Size: 988 281 1182 246 3079
Fragments arranged by size:
3079 1182 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2217 2429 748
Size: 1469 212 4095
Fragments arranged by size:
4095 1469 212
HpaI GTT'AAC
Cuts at: 3366 3366
Size: 5776
KasI G'GCGC_C
Cuts at: 2459 2459
Size: 5776
KpnI G_GTAC'C
Cuts at: 3101 3101
Size: 5776
MfeI C'AATT_G
Cuts at: 3375 3375
Size: 5776
MluI A'CGCG_T
Cuts at: 5652 5652
Size: 5776
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2110 4890 5074 1305
Size: 805 2780 184 2007
Fragments arranged by size:
2780 2007 805 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2579 2917 10
Size: 54 1425 343 747 338 2869
Fragments arranged by size:
2869 1425 747 343 338 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3734 4093 4252 518
Size: 648 2568 359 159 2042
Fragments arranged by size:
2568 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2722 3192 3869 5017 5262 1606
Size: 1116 470 677 1148 245 2120
Fragments arranged by size:
2120 1148 1116 677 470 245
NarI GG'CG_CC
Cuts at: 2460 2460
Size: 5776
NcoI C'CATG_G
Cuts at: 513 1065 2004 2199 513
Size: 552 939 195 4090
Fragments arranged by size:
4090 939 552 195
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5776
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5776
NotI GC'GGCC_GC
Cuts at: 3223 3223
Size: 5776
NsiI A_TGCA'T
Cuts at: 2124 2124
Size: 5776
NspI r_CATG'y
Cuts at: 1132 1229 2864 3134 4679 1132
Size: 97 1635 270 1545 2229
Fragments arranged by size:
2229 1635 1545 270 97
PasI CC'CwG_GG
Cuts at: 1348 2518 1348
Size: 1170 4606
PciI A'CATG_T
Cuts at: 4675 4675
Size: 5776
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5776
PfoI T'CCnGG_A
Cuts at: 2871 2871
Size: 5776
PmeI GTTT'AAAC
Cuts at: 3080 3080
Size: 5776
PmlI CAC'GTG
Cuts at: 2913 2913
Size: 5776
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3845 3877 5358 5390 3845
Size: 32 1481 32 4231
Fragments arranged by size:
4231 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2530 2808 2530
Size: 278 5498
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5776
PsiI TTA'TAA
Cuts at: 3346 3346
Size: 5776
PstI C_TGCA'G
Cuts at: 830 2501 3128 830
Size: 1671 627 3478
Fragments arranged by size:
3478 1671 627
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5776
PvuII CAG'CTG
Cuts at: 2722 2722
Size: 5776
RsrII CG'GwC_CG
Cuts at: 2051 2051
Size: 5776
SalI G'TCGA_C
Cuts at: 1936 3118 1936
Size: 1182 4594
SanDI GG'GwC_CC
Cuts at: 2808 2808
Size: 5776
SbfI CC_TGCA'GG
Cuts at: 2501 3128 2501
Size: 627 5149
ScaI AGT'ACT
Cuts at: 4024 5736 4024
Size: 1712 4064
SexAI A'CCwGG_T
Cuts at: 2266 2266
Size: 5776
SfcI C'TryA_G
Cuts at: 826 1045 2497 3124 4259 4940 5131 826
Size: 219 1452 627 1135 681 191 1471
Fragments arranged by size:
1471 1452 1135 681 627 219 191
SfoI GGC'GCC
Cuts at: 2461 2461
Size: 5776
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5776
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5776
SmaI CCC'GGG
Cuts at: 1872 3103 1872
Size: 1231 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2598 3047 3880 4781 5043 820
Size: 197 926 655 449 833 901 262 1553
Fragments arranged by size:
1553 926 901 833 655 449 262 197
SnaBI TAC'GTA
Cuts at: 493 3567 493
Size: 3074 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5776
SphI G_CATG'C
Cuts at: 1132 2864 3134 1132
Size: 1732 270 3774
Fragments arranged by size:
3774 1732 270
SspI AAT'ATT
Cuts at: 5 52 3700 5
Size: 47 3648 2081
Fragments arranged by size:
3648 2081 47
StuI AGG'CCT
Cuts at: 1517 2768 3005 1517
Size: 1251 237 4288
Fragments arranged by size:
4288 1251 237
StyI C'CwwG_G
Cuts at: 513 1065 2004 2199 2643 2679 3215 513
Size: 552 939 195 444 36 536 3074
Fragments arranged by size:
3074 939 552 536 444 195 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2987 3803 3820 3973 4158 4603 325
Size: 1255 1407 816 17 153 185 445 1498
Fragments arranged by size:
1498 1407 1255 816 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4022 5734 96
Size: 274 80 33 51 1443 2045 1712 138
Fragments arranged by size:
2045 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5736 5768 5736
Size: 32 5744
Tth111I GACn'n_nGTC
Cuts at: 1316 2441 1316
Size: 1125 4651
XbaI T'CTAG_A
Cuts at: 3112 3112
Size: 5776
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2875 2875
Size: 5776
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5776
XmaI C'CCGG_G
Cuts at: 1870 3101 1870
Size: 1231 4545
XmnI GAAnn'nnTTC
Cuts at: 3905 3905
Size: 5776
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3584 276
Size: 53 83 186 936 2050 2468
Fragments arranged by size:
2468 2050 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII RsrII SalI SanDI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BplI BsiWI
BsmBI BstZ17I DraIII EcoICRI FalI FseI FspAI NaeI
NgoMIV NruI PacI PspOMI PspXI PsrI SacI SacII
SapI SfiI SrfI SwaI