Restriction map for
pFN21AB3736
(Circular) (Six-base) MAPSORT of: pFN21AB3736.seq Check: 8404 from: 1 to: 6058
pFN21AB3736 6058 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2956 3235 2956
Size: 279 5779
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3868 278
Size: 53 83 186 936 2332 2468
Fragments arranged by size:
2468 2332 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2685 3401 1937
Size: 748 716 4594
Fragments arranged by size:
4594 748 716
Acc65I G'GTAC_C
Cuts at: 3379 3379
Size: 6058
AclI AA'CG_TT
Cuts at: 1563 4185 4558 1563
Size: 2622 373 3063
Fragments arranged by size:
3063 2622 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3143 4121 5505 5773 1632
Size: 1511 978 1384 268 1917
Fragments arranged by size:
1917 1511 1384 978 268
AfeI AGC'GCT
Cuts at: 2423 2423
Size: 6058
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5861
AflIII A'CryG_T
Cuts at: 1203 4957 5934 1203
Size: 3754 977 1327
Fragments arranged by size:
3754 1327 977
AgeI A'CCGG_T
Cuts at: 4889 4889
Size: 6058
AhdI GACnn_n'nnGTC
Cuts at: 3171 4787 3171
Size: 1616 4442
AleI CACnn'nnGTG
Cuts at: 1166 2596 1166
Size: 1430 4628
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6058
AlwNI CAG_nnn'CTG
Cuts at: 2893 3801 5373 2893
Size: 908 1572 3578
Fragments arranged by size:
3578 1572 908
ApaI G_GGCC'C
Cuts at: 2818 2818
Size: 6058
ApaLI G'TGCA_C
Cuts at: 4115 5271 4115
Size: 1156 4902
ApoI r'AATT_y
Cuts at: 1493 2228 3367 3593 3745 5854 1493
Size: 735 1139 226 152 2109 1697
Fragments arranged by size:
2109 1697 1139 735 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4612 160
Size: 872 3580 1606
Fragments arranged by size:
3580 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6058
AvaI C'yCGr_G
Cuts at: 1870 1943 3383 1870
Size: 73 1440 4545
Fragments arranged by size:
4545 1440 73
BamHI G'GATC_C
Cuts at: 3388 3755 3388
Size: 367 5691
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3379 4834 618
Size: 325 217 627 1592 1455 1842
Fragments arranged by size:
1842 1592 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2569 2818 3060 3131 3377 1391
Size: 1178 249 242 71 246 4072
Fragments arranged by size:
4072 1178 249 246 242 71
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2227 2233 928
Size: 971 328 6 4753
Fragments arranged by size:
4753 971 328 6
BbvCI CC'TCA_GC
Cuts at: 3107 3261 3107
Size: 154 5904
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4249 4283 1903
Size: 34 2312 34 3678
Fragments arranged by size:
3678 2312 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3950 5160 3950
Size: 1210 4848
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6058
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2714 3126 4669
Size: 107 122 71 631 605 1042 412 1543
Cuts at: 4669 136
Size: 1525
Fragments arranged by size:
1543 1525 1042 631 605 412 122 107
71
BglII A'GATC_T
Cuts at: 6053 6053
Size: 6058
BlpI GC'TnA_GC
Cuts at: 2946 3059 3475 2946
Size: 113 416 5529
Fragments arranged by size:
5529 416 113
Bme1580I G_kGCm'C
Cuts at: 1790 2818 4119 5275 1790
Size: 1028 1301 1156 2573
Fragments arranged by size:
2573 1301 1156 1028
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6058
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2020 2565 2970
Size: 416 53 83 399 24 603 545 405
Cuts at: 2970 3789 4747 442
Size: 819 958 1753
Fragments arranged by size:
1753 958 819 603 545 416 405 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6058
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2079 3286 2079
Size: 1207 4851
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2287 2579 3078 3302 3371 4718 1239
Size: 1048 292 499 224 69 1347 2579
Fragments arranged by size:
2579 1347 1048 499 292 224 69
Bpu10I CC'TnA_GC
Cuts at: 2809 3107 3261 2809
Size: 298 154 5606
Fragments arranged by size:
5606 298 154
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4183 5048 5346 4183
Size: 865 298 4895
Fragments arranged by size:
4895 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2554 3260 4721 882
Size: 617 1055 706 1461 2219
Fragments arranged by size:
2219 1461 1055 706 617
BsaAI yAC'GTr
Cuts at: 493 1204 2434 2745 3849 493
Size: 711 1230 311 1104 2702
Fragments arranged by size:
2702 1230 1104 711 311
BsaBI GATnn'nnATC
Cuts at: 6052 6052
Size: 6058
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2213 3865 4247 4882
Size: 53 83 186 936 680 1652 382 635
Cuts at: 4882 275
Size: 1451
Fragments arranged by size:
1652 1451 936 680 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2835 4491 4889 5163 5310 5721 2835
Size: 1656 398 274 147 411 3172
Fragments arranged by size:
3172 1656 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2593 2623 1178
Size: 30 165 30 1190 30 4613
Fragments arranged by size:
4613 1190 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2660 3113 3116 3152 3278 1186
Size: 1474 453 3 36 126 3966
Fragments arranged by size:
3966 1474 453 126 36 3
BseYI C'CCAG_C
Cuts at: 2470 3206 5261 5757 2470
Size: 736 2055 496 2771
Fragments arranged by size:
2771 2055 736 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5485
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3508 4269 5297 1540
Size: 275 186 1507 761 1028 2301
Fragments arranged by size:
2301 1507 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2569 2947 3060 4119 4204 5275 2569
Size: 378 113 1059 85 1071 3352
Fragments arranged by size:
3352 1071 1059 378 113 85
BsmI GAATG_Cn'
Cuts at: 3569 3662 3569
Size: 93 5965
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2569 2818 2947 3060 3131 3377 4119
Size: 399 779 249 129 113 71 246 742
Cuts at: 4119 4204 5275 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 779 742 399 249 246 129
113 85 71
BspEI T'CCGG_A
Cuts at: 5721 5721
Size: 6058
BspHI T'CATG_A
Cuts at: 3947 5677 3947
Size: 1730 4328
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2271 2956 3235 3413 844
Size: 1427 685 279 178 3489
Fragments arranged by size:
3489 1427 685 279 178
BsrBI CCG'CTC
Cuts at: 1394 1823 2790 3945 1394
Size: 429 967 1155 3507
Fragments arranged by size:
3507 1155 967 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2188 4553 4727 65
Size: 1183 940 2365 174 1396
Fragments arranged by size:
2365 1396 1183 940 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3039 4702 4889 1700
Size: 110 1229 1663 187 2869
Fragments arranged by size:
2869 1663 1229 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6058
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6058
BssSI C'ACGA_G
Cuts at: 4118 5130 4118
Size: 1012 5046
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6058
BstBI TT'CG_AA
Cuts at: 3241 4862 3241
Size: 1621 4437
BstEII G'GTnAC_C
Cuts at: 1184 2800 2980 1184
Size: 1616 180 4262
Fragments arranged by size:
4262 1616 180
BstXI CCAn_nnnn'nTGG
Cuts at: 2416 2749 2416
Size: 333 5725
BstYI r'GATC_y
Cuts at: 1972 3045 3388 3755 4140 4157 5609 5695 5707
Size: 1073 343 367 385 17 1452 86 12
Cuts at: 5707 6053 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1073 385 367 346 343 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2520 513
Size: 552 939 516 4051
Fragments arranged by size:
4051 939 552 516
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5319
Fragments arranged by size:
5319 683 56
BtsI GCAGTG_nn'
Cuts at: 2868 3574 4368 4388 2868
Size: 706 794 20 4538
Fragments arranged by size:
4538 794 706 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6058
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6023
DraI TTT'AAA
Cuts at: 3362 3362
Size: 6058
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5065 809
Size: 507 3749 1802
Fragments arranged by size:
3749 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2196 2292 2388
Size: 54 1425 50 275 18 366 96 96
Cuts at: 2388 2517 3505 4394 6023 8
Size: 129 988 889 1629 43
Fragments arranged by size:
1629 1425 988 889 366 275 129 96
96 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3505 1537
Size: 275 1693 4090
Fragments arranged by size:
4090 1693 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2047 2147 3988 1353
Size: 694 100 1841 3423
Fragments arranged by size:
3423 1841 694 100
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4645 5019 5165 387
Size: 1064 138 3056 374 146 1280
Fragments arranged by size:
3056 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2567 3058 2567
Size: 491 5567
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2287 2579 3078 3143 3302 3371 4121
Size: 393 655 292 499 65 159 69 750
Cuts at: 4121 4718 5505 5773 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 787 750 655 597 499 393 292
268 159 69 65
EcoO109I rG'GnC_Cy
Cuts at: 2059 2075 2095 2814 2815 2059
Size: 16 20 719 1 5302
Fragments arranged by size:
5302 719 20 16 1
EcoRI G'AATT_C
Cuts at: 3367 3745 3367
Size: 378 5680
FspI TGC'GCA
Cuts at: 2462 4564 2462
Size: 2102 3956
FspAI rTGC'GCAy
Cuts at: 2462 2462
Size: 6058
HaeII r_GCGC'y
Cuts at: 1678 2425 5205 1678
Size: 747 2780 2531
Fragments arranged by size:
2780 2531 747
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3157 3189 4699 4731 4773
Size: 32 135 32 1585 32 1510 32 42
Cuts at: 4773 4805 1373
Size: 32 2626
Fragments arranged by size:
2626 1585 1510 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3235 3402 3648 669
Size: 988 281 1297 167 246 3079
Fragments arranged by size:
3079 1297 988 281 246 167
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6058
HpaI GTT'AAC
Cuts at: 3648 3648
Size: 6058
KpnI G_GTAC'C
Cuts at: 3383 3383
Size: 6058
MfeI C'AATT_G
Cuts at: 3657 3657
Size: 6058
MluI A'CGCG_T
Cuts at: 5934 5934
Size: 6058
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3036 5172 5356 1305
Size: 1731 2136 184 2007
Fragments arranged by size:
2136 2007 1731 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2294 2390 2519 10
Size: 54 1425 343 462 96 129 3549
Fragments arranged by size:
3549 1425 462 343 129 96 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2414 2596 2678 2747 2928 3308 4016
Size: 648 1248 182 82 69 181 380 708
Cuts at: 4016 4375 4534 518
Size: 359 159 2042
Fragments arranged by size:
2042 1248 708 648 380 359 182 181
159 82 69
MspA1I CmG'CkG
Cuts at: 1606 2189 2737 3474 4151 5299 5544 1606
Size: 583 548 737 677 1148 245 2120
Fragments arranged by size:
2120 1148 737 677 583 548 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2520 513
Size: 552 939 516 4051
Fragments arranged by size:
4051 939 552 516
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6058
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6058
NotI GC'GGCC_GC
Cuts at: 3505 3505
Size: 6058
NspI r_CATG'y
Cuts at: 1132 1229 2698 3416 4961 1132
Size: 97 1469 718 1545 2229
Fragments arranged by size:
2229 1545 1469 718 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6058
PciI A'CATG_T
Cuts at: 4957 4957
Size: 6058
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6058
PfoI T'CCnGG_A
Cuts at: 2097 3279 2097
Size: 1182 4876
PmeI GTTT'AAAC
Cuts at: 3362 3362
Size: 6058
PmlI CAC'GTG
Cuts at: 2745 2745
Size: 6058
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4127 4159 5640 5672 4127
Size: 32 1481 32 4513
Fragments arranged by size:
4513 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2059 2075 2095 2059
Size: 16 20 6022
Fragments arranged by size:
6022 20 16
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6058
PsiI TTA'TAA
Cuts at: 3628 3628
Size: 6058
PspOMI G'GGCC_C
Cuts at: 2814 2814
Size: 6058
PstI C_TGCA'G
Cuts at: 830 2693 3153 3410 830
Size: 1863 460 257 3478
Fragments arranged by size:
3478 1863 460 257
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6058
SacI G_AGCT'C
Cuts at: 2569 3060 2569
Size: 491 5567
SalI G'TCGA_C
Cuts at: 1936 3400 1936
Size: 1464 4594
SanDI GG'GwC_CC
Cuts at: 2059 2095 2059
Size: 36 6022
SbfI CC_TGCA'GG
Cuts at: 3410 3410
Size: 6058
ScaI AGT'ACT
Cuts at: 4306 6018 4306
Size: 1712 4346
SfcI C'TryA_G
Cuts at: 826 1045 2689 3149 3406 4541 5222 5413 826
Size: 219 1644 460 257 1135 681 191 1471
Fragments arranged by size:
1644 1471 1135 681 460 257 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6058
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6058
SmaI CCC'GGG
Cuts at: 1872 3385 1872
Size: 1513 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4162 5063 5325 820
Size: 197 926 2219 901 262 1553
Fragments arranged by size:
2219 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2434 3849 493
Size: 1941 1415 2702
Fragments arranged by size:
2702 1941 1415
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6058
SphI G_CATG'C
Cuts at: 1132 3416 1132
Size: 2284 3774
SspI AAT'ATT
Cuts at: 5 52 3982 5
Size: 47 3930 2081
Fragments arranged by size:
3930 2081 47
StuI AGG'CCT
Cuts at: 1517 2513 1517
Size: 996 5062
StyI C'CwwG_G
Cuts at: 513 1065 2004 2514 2520 3497 513
Size: 552 939 510 6 977 3074
Fragments arranged by size:
3074 977 939 552 510 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2032 2486 2494 4085 4102 4255 4440
Size: 1255 452 454 8 1591 17 153 185
Cuts at: 4440 4885 325
Size: 445 1498
Fragments arranged by size:
1591 1498 1255 454 452 445 185 153
17 8
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4304 6016 96
Size: 274 80 33 51 1443 2327 1712 138
Fragments arranged by size:
2327 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2593 2625 6018 6050 2593
Size: 32 3393 32 2601
Fragments arranged by size:
3393 2601 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6058
XbaI T'CTAG_A
Cuts at: 3394 3394
Size: 6058
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2330 2330
Size: 6058
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6058
XmaI C'CCGG_G
Cuts at: 1870 3383 1870
Size: 1513 4545
XmnI GAAnn'nnTTC
Cuts at: 4187 4187
Size: 6058
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3866 276
Size: 53 83 186 936 2332 2468
Fragments arranged by size:
2468 2332 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI SacI SalI SanDI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI
BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI KasI
NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI
PvuII RsrII SacII SapI SexAI SfiI SfoI SrfI
SwaI