Restriction map for
pFN21AB3707
(Circular) (Six-base) MAPSORT of: pFN21AB3707.seq Check: 636 from: 1 to: 7702
pFN21AB3707 7702 bp
With 182 enzymes: SgfI *
October 20, 2009 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3130 3850 3130
Size: 720 6982
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5512 278
Size: 53 83 186 936 3976 2468
Fragments arranged by size:
3976 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3330 5045 1937
Size: 1393 1715 4594
Fragments arranged by size:
4594 1715 1393
Acc65I G'GTAC_C
Cuts at: 3767 5023 3767
Size: 1256 6446
AclI AA'CG_TT
Cuts at: 1563 4571 5829 6202 1563
Size: 3008 1258 373 3063
Fragments arranged by size:
3063 3008 1258 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2508 3215 3410 3540 4157 4869 5765 7149
Size: 876 707 195 130 617 712 896 1384
Cuts at: 7149 7417 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 896 876 712 707 617 268
195 130
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7505
AflIII A'CryG_T
Cuts at: 1203 6601 7578 1203
Size: 5398 977 1327
Fragments arranged by size:
5398 1327 977
AgeI A'CCGG_T
Cuts at: 2209 6533 2209
Size: 4324 3378
AhdI GACnn_n'nnGTC
Cuts at: 3607 6431 3607
Size: 2824 4878
AleI CACnn'nnGTG
Cuts at: 1166 2729 4376 4556 1166
Size: 1563 1647 180 4312
Fragments arranged by size:
4312 1647 1563 180
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7702
AlwNI CAG_nnn'CTG
Cuts at: 3550 4025 4717 4874 4946 5445 7017 3550
Size: 475 692 157 72 499 1572 4235
Fragments arranged by size:
4235 1572 692 499 475 157 72
ApaI G_GGCC'C
Cuts at: 3429 4522 3429
Size: 1093 6609
ApaLI G'TGCA_C
Cuts at: 2405 5759 6915 2405
Size: 3354 1156 3192
Fragments arranged by size:
3354 3192 1156
ApoI r'AATT_y
Cuts at: 1493 3044 5011 5237 5389 7498 1493
Size: 1551 1967 226 152 2109 1697
Fragments arranged by size:
2109 1967 1697 1551 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6256 160
Size: 872 5224 1606
Fragments arranged by size:
5224 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7702
AvaI C'yCGr_G
Cuts at: 1870 1943 3165 4274 4776 5027 1870
Size: 73 1222 1109 502 251 4545
Fragments arranged by size:
4545 1222 1109 502 251 73
BamHI G'GATC_C
Cuts at: 4892 5032 5399 4892
Size: 140 367 7195
Fragments arranged by size:
7195 367 140
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3079 3767 5023 6478 618
Size: 325 217 627 1292 688 1256 1455 1842
Fragments arranged by size:
1842 1455 1292 1256 688 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2708 2835 3429 3827 3870 4522 4863 5021
Size: 1317 127 594 398 43 652 341 158
Cuts at: 5021 1391
Size: 4072
Fragments arranged by size:
4072 1317 652 594 398 341 158 127
43
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2530 2641 2756 928
Size: 971 631 111 115 5874
Fragments arranged by size:
5874 971 631 115 111
BbvCI CC'TCA_GC
Cuts at: 2636 3882 4406 2636
Size: 1246 524 5932
Fragments arranged by size:
5932 1246 524
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2561 2595 5893 5927 1903
Size: 34 624 34 3298 34 3678
Fragments arranged by size:
3678 3298 624 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3606 5594 6804 3606
Size: 1988 1210 4504
Fragments arranged by size:
4504 1988 1210
BclI T'GATC_A
Cuts at: 1548 2523 1548
Size: 975 6727
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2038 2060 3257
Size: 107 122 71 631 605 366 22 1197
Cuts at: 3257 4465 4628 6313 136
Size: 1208 163 1685 1525
Fragments arranged by size:
1685 1525 1208 1197 631 605 366 163
122 107 71 22
BglII A'GATC_T
Cuts at: 3523 4314 4545 7697 3523
Size: 791 231 3152 3528
Fragments arranged by size:
3528 3152 791 231
BlpI GC'TnA_GC
Cuts at: 3281 4328 4401 4632 5119 3281
Size: 1047 73 231 487 5864
Fragments arranged by size:
5864 1047 487 231 73
Bme1580I G_kGCm'C
Cuts at: 1790 2169 2409 3429 4522 5763 6919 1790
Size: 379 240 1020 1093 1241 1156 2573
Fragments arranged by size:
2573 1241 1156 1093 1020 379 240
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7702
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3884 5433 6391
Size: 416 53 83 399 24 2467 1549 958
Cuts at: 6391 442
Size: 1753
Fragments arranged by size:
2467 1753 1549 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7702
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3919 4333 3919
Size: 414 7288
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2181 2843 3089 4023 4217 4370 4818 4956
Size: 942 662 246 934 194 153 448 138
Cuts at: 4956 6362 1239
Size: 1406 2579
Fragments arranged by size:
2579 1406 942 934 662 448 246 194
153 138
Bpu10I CC'TnA_GC
Cuts at: 2636 2707 3882 4406 2636
Size: 71 1175 524 5932
Fragments arranged by size:
5932 1175 524 71
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3854 4496 5827 6692 6990 3854
Size: 642 1331 865 298 4566
Fragments arranged by size:
4566 1331 865 642 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4185 4244 4586 6365 882
Size: 617 2686 59 342 1779 2219
Fragments arranged by size:
2686 2219 1779 617 342 59
BsaAI yAC'GTr
Cuts at: 493 1204 5493 493
Size: 711 4289 2702
Fragments arranged by size:
4289 2702 711
BsaBI GATnn'nnATC
Cuts at: 7696 7696
Size: 7702
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4032 5509 5891 6526
Size: 53 83 186 936 2499 1477 382 635
Cuts at: 6526 275
Size: 1451
Fragments arranged by size:
2499 1477 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2209 6135 6533 6807 6954 7365 2209
Size: 3926 398 274 147 411 2546
Fragments arranged by size:
3926 2546 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7477
Fragments arranged by size:
7477 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2050 3065 3227 3380 3383 3833 4109 4313
Size: 864 1015 162 153 3 450 276 204
Cuts at: 4313 4325 4457 4478 1186
Size: 12 132 21 4410
Fragments arranged by size:
4410 1015 864 450 276 204 162 153
132 21 12 3
BseYI C'CCAG_C
Cuts at: 2762 2855 3217 6905 7401 2762
Size: 93 362 3688 496 3063
Fragments arranged by size:
3688 3063 496 362 93
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2277 3081 4184 4730 1163
Size: 573 541 804 1103 546 4135
Fragments arranged by size:
4135 1103 804 573 546 541
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5152 5913 6941 1540
Size: 275 186 3151 761 1028 2301
Fragments arranged by size:
3151 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2070 2373 2409 2835 3657 3870 5763 5848 6919
Size: 303 36 426 822 213 1893 85 1071
Cuts at: 6919 2070
Size: 2853
Fragments arranged by size:
2853 1893 1071 822 426 303 213 85
36
BsmI GAATG_Cn'
Cuts at: 4615 5213 5306 4615
Size: 598 93 7011
Fragments arranged by size:
7011 598 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2070 2169 2373 2409 2708 2835 3429
Size: 399 280 99 204 36 299 127 594
Cuts at: 3429 3657 3827 3870 4522 4863 5021 5763 5848
Size: 228 170 43 652 341 158 742 85
Cuts at: 5848 6919 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 652 594 399 341 299
280 228 204 170 158 127 99 85
43 36
BspEI T'CCGG_A
Cuts at: 7365 7365
Size: 7702
BspHI T'CATG_A
Cuts at: 5591 7321 5591
Size: 1730 5972
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3130 3850 4134 5057 844
Size: 2286 720 284 923 3489
Fragments arranged by size:
3489 2286 923 720 284
BsrBI CCG'CTC
Cuts at: 1394 1823 3076 5589 1394
Size: 429 1253 2513 3507
Fragments arranged by size:
3507 2513 1253 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6197 6371 65
Size: 1183 4949 174 1396
Fragments arranged by size:
4949 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2209 6346 6533 1700
Size: 110 399 4137 187 2869
Fragments arranged by size:
4137 2869 399 187 110
BsrGI T'GTAC_A
Cuts at: 96 3091 96
Size: 2995 4707
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7702
BssSI C'ACGA_G
Cuts at: 2372 3036 3352 5762 6774 2372
Size: 664 316 2410 1012 3300
Fragments arranged by size:
3300 2410 1012 664 316
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2404 2721 4717 794
Size: 1610 317 1996 3779
Fragments arranged by size:
3779 1996 1610 317
BstBI TT'CG_AA
Cuts at: 6506 6506
Size: 7702
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7702
BstXI CCAn_nnnn'nTGG
Cuts at: 2143 3343 4231 4348 2143
Size: 1200 888 117 5497
Fragments arranged by size:
5497 1200 888 117
BstYI r'GATC_y
Cuts at: 1972 2784 3523 4314 4545 4892 5032 5399 5784
Size: 812 739 791 231 347 140 367 385
Cuts at: 5784 5801 7253 7339 7351 7697 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 812 791 739 385 367 347
346 231 140 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2442 3927 3945 4026 4758 2442
Size: 1485 18 81 732 5386
Fragments arranged by size:
5386 1485 732 81 18
BtgI C'CryG_G
Cuts at: 513 1065 2004 2169 2919 3201 3252 513
Size: 552 939 165 750 282 51 4963
Fragments arranged by size:
4963 939 750 552 282 165 51
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6963
Fragments arranged by size:
6963 683 56
BtsI GCAGTG_nn'
Cuts at: 2112 2134 2526 2628 2673 3267 3330 5218 6012
Size: 22 392 102 45 594 63 1888 794
Cuts at: 6012 6032 2112
Size: 20 3782
Fragments arranged by size:
3782 1888 794 594 392 102 63 45
22 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7702
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7667
DraI TTT'AAA
Cuts at: 3295 5006 3295
Size: 1711 5991
DraIII CAC_nnn'GTG
Cuts at: 2093 2093
Size: 7702
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6709 809
Size: 507 5393 1802
Fragments arranged by size:
5393 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2145 2922 4620
Size: 54 1425 50 275 18 315 777 1698
Cuts at: 4620 5149 6038 7667 8
Size: 529 889 1629 43
Fragments arranged by size:
1698 1629 1425 889 777 529 315 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5149 1537
Size: 275 3337 4090
Fragments arranged by size:
4090 3337 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3416 3705 3810 5632 1353
Size: 2063 289 105 1822 3423
Fragments arranged by size:
3423 2063 1822 289 105
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2642 6289 6663 6809 387
Size: 1064 138 1053 3647 374 146 1280
Fragments arranged by size:
3647 1280 1064 1053 374 146 138
EcoICRI GAG'CTC
Cuts at: 2833 3868 2833
Size: 1035 6667
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2181 2508 2843 3089 3215 3410 3540
Size: 393 549 327 335 246 126 195 130
Cuts at: 3540 4023 4157 4217 4370 4818 4869 4956 5765
Size: 483 134 60 153 448 51 87 809
Cuts at: 5765 6362 7149 7417 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 809 787 597 549 483 448 393
335 327 268 246 195 153 134 130
126 87 60 51
EcoNI CCTnn'n_nnAGG
Cuts at: 4836 4836
Size: 7702
EcoO109I rG'GnC_Cy
Cuts at: 2818 3100 3169 3425 3426 3515 3886 4022 4518
Size: 282 69 256 1 89 371 136 496
Cuts at: 4518 4773 4931 2818
Size: 255 158 5589
Fragments arranged by size:
5589 496 371 282 256 255 158 136
89 69 1
EcoRI G'AATT_C
Cuts at: 5011 5389 5011
Size: 378 7324
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3646 3646
Size: 7702
FspI TGC'GCA
Cuts at: 6208 6208
Size: 7702
HaeII r_GCGC'y
Cuts at: 1678 2560 4335 6849 1678
Size: 882 1775 2514 2531
Fragments arranged by size:
2531 2514 1775 882
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2263 2295 2690 2722 3593
Size: 32 135 32 691 32 395 32 871
Cuts at: 3593 3625 4877 4909 6343 6375 6417 6449 1373
Size: 32 1252 32 1434 32 42 32 2626
Fragments arranged by size:
2626 1434 1252 871 691 395 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5046 5292 669
Size: 988 281 3108 246 3079
Fragments arranged by size:
3108 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7702
HpaI GTT'AAC
Cuts at: 5292 5292
Size: 7702
KpnI G_GTAC'C
Cuts at: 3771 5027 3771
Size: 1256 6446
MfeI C'AATT_G
Cuts at: 5301 5301
Size: 7702
MluI A'CGCG_T
Cuts at: 7578 7578
Size: 7702
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4345 6816 7000 1305
Size: 3040 2471 184 2007
Fragments arranged by size:
3040 2471 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2147 2924 10
Size: 54 1425 343 315 777 4788
Fragments arranged by size:
4788 1425 777 343 315 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2141 2729 3341 4376 4379 4556 5660
Size: 648 975 588 612 1035 3 177 1104
Cuts at: 5660 6019 6178 518
Size: 359 159 2042
Fragments arranged by size:
2042 1104 1035 975 648 612 588 359
177 159 3
MspA1I CmG'CkG
Cuts at: 1606 2428 2779 3275 5118 5795 6943 7188 1606
Size: 822 351 496 1843 677 1148 245 2120
Fragments arranged by size:
2120 1843 1148 822 677 496 351 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2169 2919 3252 513
Size: 552 939 165 750 333 4963
Fragments arranged by size:
4963 939 750 552 333 165
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7702
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7702
NotI GC'GGCC_GC
Cuts at: 5149 5149
Size: 7702
NspI r_CATG'y
Cuts at: 1132 1229 4507 5060 6605 1132
Size: 97 3278 553 1545 2229
Fragments arranged by size:
3278 2229 1545 553 97
PasI CC'CwG_GG
Cuts at: 1348 2812 1348
Size: 1464 6238
PciI A'CATG_T
Cuts at: 6601 6601
Size: 7702
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7702
PfoI T'CCnGG_A
Cuts at: 2364 3807 4933 2364
Size: 1443 1126 5133
Fragments arranged by size:
5133 1443 1126
PmeI GTTT'AAAC
Cuts at: 5006 5006
Size: 7702
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5771 5803 7284 7316 5771
Size: 32 1481 32 6157
Fragments arranged by size:
6157 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3169 4773 4931 3169
Size: 1604 158 5940
Fragments arranged by size:
5940 1604 158
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7702
PsiI TTA'TAA
Cuts at: 5272 5272
Size: 7702
PspOMI G'GGCC_C
Cuts at: 3425 4518 3425
Size: 1093 6609
PstI C_TGCA'G
Cuts at: 830 2433 2574 2607 2784 3832 5054 830
Size: 1603 141 33 177 1048 1222 3478
Fragments arranged by size:
3478 1603 1222 1048 177 141 33
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7702
PvuII CAG'CTG
Cuts at: 2428 2779 3275 2428
Size: 351 496 6855
Fragments arranged by size:
6855 496 351
SacI G_AGCT'C
Cuts at: 2835 3870 2835
Size: 1035 6667
SalI G'TCGA_C
Cuts at: 1936 5044 1936
Size: 3108 4594
SanDI GG'GwC_CC
Cuts at: 3169 4773 3169
Size: 1604 6098
SbfI CC_TGCA'GG
Cuts at: 5054 5054
Size: 7702
ScaI AGT'ACT
Cuts at: 5950 7662 5950
Size: 1712 5990
SfcI C'TryA_G
Cuts at: 826 1045 2429 2570 2603 2780 3463 3828 5050
Size: 219 1384 141 33 177 683 365 1222
Cuts at: 5050 6185 6866 7057 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1384 1222 1135 683 681 365 219
191 177 141 33
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7702
SgrAI Cr'CCGG_yG
Cuts at: 1700 2209 1700
Size: 509 7193
SmaI CCC'GGG
Cuts at: 1872 4778 5029 1872
Size: 2906 251 4545
Fragments arranged by size:
4545 2906 251
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3869 4511 5806 6707 6969 820
Size: 197 926 1926 642 1295 901 262 1553
Fragments arranged by size:
1926 1553 1295 926 901 642 262 197
SnaBI TAC'GTA
Cuts at: 493 5493 493
Size: 5000 2702
SpeI A'CTAG_T
Cuts at: 152 4999 152
Size: 4847 2855
SphI G_CATG'C
Cuts at: 1132 5060 1132
Size: 3928 3774
SspI AAT'ATT
Cuts at: 5 52 5626 5
Size: 47 5574 2081
Fragments arranged by size:
5574 2081 47
StuI AGG'CCT
Cuts at: 1517 3000 1517
Size: 1483 6219
StyI C'CwwG_G
Cuts at: 513 1065 2004 2055 2169 2919 3252 3612 3783
Size: 552 939 51 114 750 333 360 171
Cuts at: 3783 4420 5141 513
Size: 637 721 3074
Fragments arranged by size:
3074 939 750 721 637 552 360 333
171 114 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3143 3566 4716 5729 5746 5899 6084
Size: 1255 1563 423 1150 1013 17 153 185
Cuts at: 6084 6529 325
Size: 445 1498
Fragments arranged by size:
1563 1498 1255 1150 1013 445 423 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3091 5948 7660
Size: 274 80 33 51 1443 1114 2857 1712
Cuts at: 7660 96
Size: 138
Fragments arranged by size:
2857 1712 1443 1114 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7662 7694 7662
Size: 32 7670
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7702
XbaI T'CTAG_A
Cuts at: 3661 5038 3661
Size: 1377 6325
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7702
XmaI C'CCGG_G
Cuts at: 1870 4776 5027 1870
Size: 2906 251 4545
Fragments arranged by size:
4545 2906 251
XmnI GAAnn'nnTTC
Cuts at: 3525 5831 3525
Size: 2306 5396
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5510 276
Size: 53 83 186 936 3976 2468
Fragments arranged by size:
3976 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SanDI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
BsmBI BstZ17I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII
SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI