Restriction map for
pFN21AB3705
(Circular) (Six-base) MAPSORT of: pFN21AB3705.seq Check: 7760 from: 1 to: 7915
pFN21AB3705 7915 bp
With 182 enzymes: SgfI *
February 27, 2009 14:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5725 278
Size: 53 83 186 936 4189 2468
Fragments arranged by size:
4189 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4371 5258 1937
Size: 2434 887 4594
Fragments arranged by size:
4594 2434 887
Acc65I G'GTAC_C
Cuts at: 2140 5236 2140
Size: 3096 4819
AclI AA'CG_TT
Cuts at: 1563 2695 6042 6415 1563
Size: 1132 3347 373 3063
Fragments arranged by size:
3347 3063 1132 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 1996 2454 3761 3773 5978 7362 7630 1632
Size: 364 458 1307 12 2205 1384 268 1917
Fragments arranged by size:
2205 1917 1384 1307 458 364 268 12
AflII C'TTAA_G
Cuts at: 820 1017 5075 820
Size: 197 4058 3660
Fragments arranged by size:
4058 3660 197
AflIII A'CryG_T
Cuts at: 1203 2628 6814 7791 1203
Size: 1425 4186 977 1327
Fragments arranged by size:
4186 1425 1327 977
AgeI A'CCGG_T
Cuts at: 6746 6746
Size: 7915
AhdI GACnn_n'nnGTC
Cuts at: 4279 6644 4279
Size: 2365 5550
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7915
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4074 1184
Size: 2890 5025
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2538 2570 2538
Size: 32 7883
AlwNI CAG_nnn'CTG
Cuts at: 3784 5658 7230 3784
Size: 1874 1572 4469
Fragments arranged by size:
4469 1874 1572
ApaLI G'TGCA_C
Cuts at: 4049 5972 7128 4049
Size: 1923 1156 4836
Fragments arranged by size:
4836 1923 1156
ApoI r'AATT_y
Cuts at: 1493 2411 2444 2465 2923 3161 3485 4703 4834
Size: 918 33 21 458 238 324 1218 131
Cuts at: 4834 5135 5224 5450 5602 7711 1493
Size: 301 89 226 152 2109 1697
Fragments arranged by size:
2109 1697 1218 918 458 324 301 238
226 152 131 89 33 21
AseI AT'TA_AT
Cuts at: 160 1032 3171 3264 6469 160
Size: 872 2139 93 3205 1606
Fragments arranged by size:
3205 2139 1606 872 93
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7915
AvaI C'yCGr_G
Cuts at: 1870 1943 2145 3813 5240 1870
Size: 73 202 1668 1427 4545
Fragments arranged by size:
4545 1668 1427 202 73
BamHI G'GATC_C
Cuts at: 2428 3637 5245 5612 2428
Size: 1209 1608 367 4731
Fragments arranged by size:
4731 1608 1209 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2140 5236 6691 618
Size: 325 217 627 353 3096 1455 1842
Fragments arranged by size:
3096 1842 1455 627 353 325 217
BanII G_rGCy'C
Cuts at: 1391 2836 3093 5234 1391
Size: 1445 257 2141 4072
Fragments arranged by size:
4072 2141 1445 257
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2302 3384 928
Size: 971 403 1082 5459
Fragments arranged by size:
5459 1082 971 403
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3678 3712 6106 6140 1903
Size: 34 1741 34 2394 34 3678
Fragments arranged by size:
3678 2394 1741 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5807 7017 5807
Size: 1210 6705
BclI T'GATC_A
Cuts at: 1548 2782 3136 1548
Size: 1234 354 6327
Fragments arranged by size:
6327 1234 354
BfrBI ATG'CAT
Cuts at: 4231 4970 4231
Size: 739 7176
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2906 4363 6526
Size: 107 122 71 631 605 1234 1457 2163
Cuts at: 6526 136
Size: 1525
Fragments arranged by size:
2163 1525 1457 1234 631 605 122 107
71
BglII A'GATC_T
Cuts at: 5210 7910 5210
Size: 2700 5215
BlpI GC'TnA_GC
Cuts at: 3771 5332 3771
Size: 1561 6354
Bme1580I G_kGCm'C
Cuts at: 1790 3970 4053 4119 5976 7132 1790
Size: 2180 83 66 1857 1156 2573
Fragments arranged by size:
2573 2180 1857 1156 83 66
BmgBI CAC'GTC
Cuts at: 1318 2629 1318
Size: 1311 6604
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2127 5646 6604
Size: 416 53 83 399 24 710 3519 958
Cuts at: 6604 442
Size: 1753
Fragments arranged by size:
3519 1753 958 710 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7915
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3387 6575 1239
Size: 2148 3188 2579
Fragments arranged by size:
3188 2579 2148
Bpu10I CC'TnA_GC
Cuts at: 5150 5150
Size: 7915
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3779 6040 6905 7203 3779
Size: 2261 865 298 4491
Fragments arranged by size:
4491 2261 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6578 882
Size: 617 5079 2219
Fragments arranged by size:
5079 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5706 493
Size: 711 4502 2702
Fragments arranged by size:
4502 2702 711
BsaBI GATnn'nnATC
Cuts at: 2126 4971 7909 2126
Size: 2845 2938 2132
Fragments arranged by size:
2938 2845 2132
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5722 6104 6739 275
Size: 53 83 186 936 4189 382 635 1451
Fragments arranged by size:
4189 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2811 6348 6746 7020 7167 7578 2811
Size: 3537 398 274 147 411 3148
Fragments arranged by size:
3537 3148 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7690
Fragments arranged by size:
7690 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2288 1186
Size: 1102 6813
BseYI C'CCAG_C
Cuts at: 7118 7614 7118
Size: 496 7419
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 7342
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5365 6126 7154 1540
Size: 275 186 3364 761 1028 2301
Fragments arranged by size:
3364 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3093 3997 4053 4571 5976 6061 7132 3093
Size: 904 56 518 1405 85 1071 3876
Fragments arranged by size:
3876 1405 1071 904 518 85 56
BsmI GAATG_Cn'
Cuts at: 2864 4195 5426 5519 2864
Size: 1331 1231 93 5260
Fragments arranged by size:
5260 1331 1231 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2836 3093 3970 3997 4053 4119 4571
Size: 399 1046 257 877 27 56 66 452
Cuts at: 4571 5234 5976 6061 7132 1391
Size: 663 742 85 1071 2174
Fragments arranged by size:
2174 1071 1046 877 742 663 452 399
257 85 66 56 27
BspEI T'CCGG_A
Cuts at: 2811 7578 2811
Size: 4767 3148
BspHI T'CATG_A
Cuts at: 5804 7534 5804
Size: 1730 6185
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4129 4284 5270 844
Size: 3285 155 986 3489
Fragments arranged by size:
3489 3285 986 155
BsrBI CCG'CTC
Cuts at: 1394 1823 5802 1394
Size: 429 3979 3507
Fragments arranged by size:
3979 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4615 6410 6584 65
Size: 1183 3367 1795 174 1396
Fragments arranged by size:
3367 1795 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4944 6559 6746 1700
Size: 110 3134 1615 187 2869
Fragments arranged by size:
3134 2869 1615 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7915
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7915
BssSI C'ACGA_G
Cuts at: 5975 6987 5975
Size: 1012 6903
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7915
BstBI TT'CG_AA
Cuts at: 2449 6719 2449
Size: 4270 3645
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7915
BstXI CCAn_nnnn'nTGG
Cuts at: 2098 2098
Size: 7915
BstYI r'GATC_y
Cuts at: 1972 2428 3637 3670 4501 5210 5245 5612 5997
Size: 456 1209 33 831 709 35 367 385
Cuts at: 5997 6014 7466 7552 7564 7910 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1209 831 709 456 385 367
346 86 35 33 17 12
BstZ17I GTA'TAC
Cuts at: 4372 4372
Size: 7915
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 7363
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7176
Fragments arranged by size:
7176 683 56
BtsI GCAGTG_nn'
Cuts at: 3571 3719 5431 6225 6245 3571
Size: 148 1712 794 20 5241
Fragments arranged by size:
5241 1712 794 148 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7915
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7880
DraI TTT'AAA
Cuts at: 3349 4170 4242 4417 5219 3349
Size: 821 72 175 802 6045
Fragments arranged by size:
6045 821 802 175 72
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6922 809
Size: 507 5606 1802
Fragments arranged by size:
5606 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4795 4942 5362
Size: 54 1425 50 275 18 2965 147 420
Cuts at: 5362 6251 7880 8
Size: 889 1629 43
Fragments arranged by size:
2965 1629 1425 889 420 275 147 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5362 1537
Size: 275 3550 4090
Fragments arranged by size:
4090 3550 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2946 4808 5006 5093 5845 1353
Size: 1593 1862 198 87 752 3423
Fragments arranged by size:
3423 1862 1593 752 198 87
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4940 6502 6876 7022 387
Size: 1064 138 3351 1562 374 146 1280
Fragments arranged by size:
3351 1562 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3091 3091
Size: 7915
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 1996 2454 3387 3761 3773 5978 6575
Size: 393 364 458 933 374 12 2205 597
Cuts at: 6575 7362 7630 1239
Size: 787 268 1524
Fragments arranged by size:
2205 1524 933 787 597 458 393 374
364 268 12
EcoO109I rG'GnC_Cy
Cuts at: 3820 3820
Size: 7915
EcoRI G'AATT_C
Cuts at: 5224 5602 5224
Size: 378 7537
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2594 4661 2594
Size: 2067 5848
FspI TGC'GCA
Cuts at: 2462 6421 2462
Size: 3959 3956
HaeII r_GCGC'y
Cuts at: 1678 3785 5150 7062 1678
Size: 2107 1365 1912 2531
Fragments arranged by size:
2531 2107 1912 1365
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2216 2248 3629 3661 4265
Size: 32 135 32 644 32 1381 32 604
Cuts at: 4265 4297 5195 5227 6556 6588 6630 6662 1373
Size: 32 898 32 1329 32 42 32 2626
Fragments arranged by size:
2626 1381 1329 898 644 604 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4449 5028 5259 5505 669
Size: 988 281 2511 579 231 246 3079
Fragments arranged by size:
3079 2511 988 579 281 246 231
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7915
HpaI GTT'AAC
Cuts at: 5505 5505
Size: 7915
KpnI G_GTAC'C
Cuts at: 2144 5240 2144
Size: 3096 4819
MfeI C'AATT_G
Cuts at: 3356 4581 5514 3356
Size: 1225 933 5757
Fragments arranged by size:
5757 1225 933
MluI A'CGCG_T
Cuts at: 7791 7791
Size: 7915
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2115 4384 7029 7213 1305
Size: 810 2269 2645 184 2007
Fragments arranged by size:
2645 2269 2007 810 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4797 10
Size: 54 1425 343 2965 3128
Fragments arranged by size:
3128 2965 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2096 4124 5873 6232 6391 518
Size: 648 930 2028 1749 359 159 2042
Fragments arranged by size:
2042 2028 1749 930 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2757 3387 3551 4597 5331 6008 7156 7401
Size: 1151 630 164 1046 734 677 1148 245
Cuts at: 7401 1606
Size: 2120
Fragments arranged by size:
2120 1151 1148 1046 734 677 630 245
164
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7363
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7915
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7915
NotI GC'GGCC_GC
Cuts at: 5362 5362
Size: 7915
NsiI A_TGCA'T
Cuts at: 4233 4972 4233
Size: 739 7176
NspI r_CATG'y
Cuts at: 1132 1229 2250 3482 5273 6818 1132
Size: 97 1021 1232 1791 1545 2229
Fragments arranged by size:
2229 1791 1545 1232 1021 97
PasI CC'CwG_GG
Cuts at: 1348 2357 1348
Size: 1009 6906
PciI A'CATG_T
Cuts at: 6814 6814
Size: 7915
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7915
PmeI GTTT'AAAC
Cuts at: 5219 5219
Size: 7915
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5984 6016 7497 7529 5984
Size: 32 1481 32 6370
Fragments arranged by size:
6370 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7915
PsiI TTA'TAA
Cuts at: 5485 5485
Size: 7915
PstI C_TGCA'G
Cuts at: 830 4281 4624 5035 5267 830
Size: 3451 343 411 232 3478
Fragments arranged by size:
3478 3451 411 343 232
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7915
PvuII CAG'CTG
Cuts at: 3387 3551 4597 3387
Size: 164 1046 6705
Fragments arranged by size:
6705 1046 164
SacI G_AGCT'C
Cuts at: 3093 3093
Size: 7915
SalI G'TCGA_C
Cuts at: 1936 5257 1936
Size: 3321 4594
SapI GCTCTTCn'nnn_
Cuts at: 4808 4808
Size: 7915
SbfI CC_TGCA'GG
Cuts at: 5267 5267
Size: 7915
ScaI AGT'ACT
Cuts at: 6163 7875 6163
Size: 1712 6203
SfcI C'TryA_G
Cuts at: 826 1045 3315 3873 4277 4620 5031 5206 5263
Size: 219 2270 558 404 343 411 175 57
Cuts at: 5263 6398 7079 7270 826
Size: 1135 681 191 1471
Fragments arranged by size:
2270 1471 1135 681 558 411 404 343
219 191 175 57
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7915
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7915
SmaI CCC'GGG
Cuts at: 1872 3815 5242 1872
Size: 1943 1427 4545
Fragments arranged by size:
4545 1943 1427
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3758 5075 6019 6920 7182 820
Size: 197 926 1815 1317 944 901 262 1553
Fragments arranged by size:
1815 1553 1317 944 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5706 493
Size: 5213 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7915
SphI G_CATG'C
Cuts at: 1132 5273 1132
Size: 4141 3774
SspI AAT'ATT
Cuts at: 5 52 3175 3378 5839 5
Size: 47 3123 203 2461 2081
Fragments arranged by size:
3123 2461 2081 203 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7915
StyI C'CwwG_G
Cuts at: 513 1065 4747 5354 513
Size: 552 3682 607 3074
Fragments arranged by size:
3682 3074 607 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4704 5942 5959 6112 6297 6742 325
Size: 1255 3124 1238 17 153 185 445 1498
Fragments arranged by size:
3124 1498 1255 1238 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4069 6161 7873
Size: 274 80 33 51 1443 2092 2092 1712
Cuts at: 7873 96
Size: 138
Fragments arranged by size:
2092 2092 1712 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7875 7907 7875
Size: 32 7883
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7915
XbaI T'CTAG_A
Cuts at: 3974 5251 3974
Size: 1277 6638
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4814 4814
Size: 7915
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7915
XmaI C'CCGG_G
Cuts at: 1870 3813 5240 1870
Size: 1943 1427 4545
Fragments arranged by size:
4545 1943 1427
XmnI GAAnn'nnTTC
Cuts at: 2971 5000 6044 2971
Size: 2029 1044 4842
Fragments arranged by size:
4842 2029 1044
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5723 276
Size: 53 83 186 936 4189 2468
Fragments arranged by size:
4189 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BplI BsiWI BsmBI Bsu36I DraIII EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI
SexAI SfiI SfoI SrfI SwaI