Restriction map for
pFN21AB3702
(Circular) (Six-base) MAPSORT of: pFN21AB3702.seq Check: 2227 from: 1 to: 7819
pFN21AB3702 7819 bp
With 182 enzymes: SgfI *
February 27, 2009 14:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2207 2207
Size: 7819
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5629 278
Size: 53 83 186 936 4093 2468
Fragments arranged by size:
4093 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3216 3569 4864 5162 1937
Size: 1279 353 1295 298 4594
Fragments arranged by size:
4594 1295 1279 353 298
Acc65I G'GTAC_C
Cuts at: 5140 5140
Size: 7819
AclI AA'CG_TT
Cuts at: 1563 5946 6319 1563
Size: 4383 373 3063
Fragments arranged by size:
4383 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 5882 7266 7534 1632
Size: 4250 1384 268 1917
Fragments arranged by size:
4250 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 4946 820
Size: 197 3929 3693
Fragments arranged by size:
3929 3693 197
AflIII A'CryG_T
Cuts at: 1203 3219 4924 6718 7695 1203
Size: 2016 1705 1794 977 1327
Fragments arranged by size:
2016 1794 1705 1327 977
AgeI A'CCGG_T
Cuts at: 6650 6650
Size: 7819
AhdI GACnn_n'nnGTC
Cuts at: 6548 6548
Size: 7819
AleI CACnn'nnGTG
Cuts at: 1166 2889 1166
Size: 1723 6096
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7819
AlwNI CAG_nnn'CTG
Cuts at: 2480 2687 4784 5562 7134 2480
Size: 207 2097 778 1572 3165
Fragments arranged by size:
3165 2097 1572 778 207
ApaI G_GGCC'C
Cuts at: 4534 4534
Size: 7819
ApaLI G'TGCA_C
Cuts at: 5876 7032 5876
Size: 1156 6663
ApoI r'AATT_y
Cuts at: 1493 2392 2719 3757 4294 4319 5128 5354 5506
Size: 899 327 1038 537 25 809 226 152
Cuts at: 5506 7615 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1038 899 809 537 327 226
152 25
AseI AT'TA_AT
Cuts at: 160 1032 4584 6373 160
Size: 872 3552 1789 1606
Fragments arranged by size:
3552 1789 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7819
AvaI C'yCGr_G
Cuts at: 1870 1943 5144 1870
Size: 73 3201 4545
Fragments arranged by size:
4545 3201 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3281 3314 3281
Size: 33 7786
BamHI G'GATC_C
Cuts at: 5149 5516 5149
Size: 367 7452
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2035 3154 4956 5140 6595
Size: 325 217 627 248 1119 1802 184 1455
Cuts at: 6595 618
Size: 1842
Fragments arranged by size:
1842 1802 1455 1119 627 325 248 217
184
BanII G_rGCy'C
Cuts at: 1391 3551 4311 4534 5138 1391
Size: 2160 760 223 604 4072
Fragments arranged by size:
4072 2160 760 604 223
BbeI G_GCGC'C
Cuts at: 4960 4960
Size: 7819
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2543 3453 3669 3993 928
Size: 971 644 910 216 324 4754
Fragments arranged by size:
4754 971 910 644 324 216
BbvCI CC'TCA_GC
Cuts at: 3495 3495
Size: 7819
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6010 6044 1903
Size: 34 4073 34 3678
Fragments arranged by size:
4073 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2510 5711 6921 2510
Size: 3201 1210 3408
Fragments arranged by size:
3408 3201 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7819
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6430 136
Size: 107 122 71 631 605 4758 1525
Fragments arranged by size:
4758 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4227 7814 4227
Size: 3587 4232
BlpI GC'TnA_GC
Cuts at: 5236 5236
Size: 7819
Bme1580I G_kGCm'C
Cuts at: 1790 2040 4534 5880 7036 1790
Size: 250 2494 1346 1156 2573
Fragments arranged by size:
2573 2494 1346 1156 250
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7819
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2905 5550 6508
Size: 416 53 83 399 24 1488 2645 958
Cuts at: 6508 442
Size: 1753
Fragments arranged by size:
2645 1753 1488 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7819
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4383 6479 1239
Size: 3144 2096 2579
Fragments arranged by size:
3144 2579 2096
Bpu10I CC'TnA_GC
Cuts at: 3495 3495
Size: 7819
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3205 3416 4019 5944 6809 7107 3205
Size: 211 603 1925 865 298 3917
Fragments arranged by size:
3917 1925 865 603 298 211
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2265 2529 3373 4258 6482 882
Size: 617 766 264 844 885 2224 2219
Fragments arranged by size:
2224 2219 885 844 766 617 264
BsaAI yAC'GTr
Cuts at: 493 1204 5610 493
Size: 711 4406 2702
Fragments arranged by size:
4406 2702 711
BsaBI GATnn'nnATC
Cuts at: 2121 4053 7813 2121
Size: 1932 3760 2127
Fragments arranged by size:
3760 2127 1932
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4957 5626 6008 6643
Size: 53 83 186 936 3424 669 382 635
Cuts at: 6643 275
Size: 1451
Fragments arranged by size:
3424 1451 936 669 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 6252 6650 6924 7071 7482 6252
Size: 398 274 147 411 6589
Fragments arranged by size:
6589 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2299 2302 2329 2332 1178
Size: 30 165 30 896 3 27 3 6665
Fragments arranged by size:
6665 896 165 30 30 27 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2307 2982 3640 1186
Size: 1121 675 658 5365
Fragments arranged by size:
5365 1121 675 658
BseYI C'CCAG_C
Cuts at: 3800 7022 7518 3800
Size: 3222 496 4101
Fragments arranged by size:
4101 3222 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3468 1163
Size: 573 1732 5514
Fragments arranged by size:
5514 1732 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5269 6030 7058 1540
Size: 275 186 3268 761 1028 2301
Fragments arranged by size:
3268 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4068 4810 5880 5965 7036 4068
Size: 742 1070 85 1071 4851
Fragments arranged by size:
4851 1071 1070 742 85
BsmI GAATG_Cn'
Cuts at: 3712 5330 5423 3712
Size: 1618 93 6108
Fragments arranged by size:
6108 1618 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4612 4612
Size: 7819
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2040 3551 4068 4311 4534 4810 5138
Size: 399 250 1511 517 243 223 276 328
Cuts at: 5138 5880 5965 7036 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1511 1071 742 517 399 328 276
250 243 223 85
BspEI T'CCGG_A
Cuts at: 7482 7482
Size: 7819
BspHI T'CATG_A
Cuts at: 2161 5708 7438 2161
Size: 3547 1730 2542
Fragments arranged by size:
3547 2542 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2207 2992 3343 4424 5174 844
Size: 1363 785 351 1081 750 3489
Fragments arranged by size:
3489 1363 1081 785 750 351
BsrBI CCG'CTC
Cuts at: 1394 1823 5706 1394
Size: 429 3883 3507
Fragments arranged by size:
3883 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2460 2772 6314 6488 65
Size: 1183 1212 312 3542 174 1396
Fragments arranged by size:
3542 1396 1212 1183 312 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6463 6650 1700
Size: 110 4653 187 2869
Fragments arranged by size:
4653 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3263 96
Size: 3167 4652
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7819
BssSI C'ACGA_G
Cuts at: 2964 3359 5879 6891 2964
Size: 395 2520 1012 3892
Fragments arranged by size:
3892 2520 1012 395
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3491 794
Size: 2697 5122
BstBI TT'CG_AA
Cuts at: 6623 6623
Size: 7819
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7819
BstXI CCAn_nnnn'nTGG
Cuts at: 2062 3399 2062
Size: 1337 6482
BstYI r'GATC_y
Cuts at: 1972 2116 2272 2858 4227 4780 4828 5149 5516
Size: 144 156 586 1369 553 48 321 367
Cuts at: 5516 5901 5918 7370 7456 7468 7814 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1369 586 553 385 367 346
321 156 144 86 48 17 12
BstZ17I GTA'TAC
Cuts at: 4865 4865
Size: 7819
BtgI C'CryG_G
Cuts at: 513 1065 2048 5085 513
Size: 552 983 3037 3247
Fragments arranged by size:
3247 3037 983 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7080
Fragments arranged by size:
7080 683 56
BtsI GCAGTG_nn'
Cuts at: 2070 2805 3426 5046 5073 5335 6129 6149 2070
Size: 735 621 1620 27 262 794 20 3740
Fragments arranged by size:
3740 1620 794 735 621 262 27 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7819
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3919 3954 529
Size: 35 3355 35 4394
Fragments arranged by size:
4394 3355 35 35
DraI TTT'AAA
Cuts at: 2113 3006 5123 2113
Size: 893 2117 4809
Fragments arranged by size:
4809 2117 893
DraIII CAC_nnn'GTG
Cuts at: 3491 4760 3491
Size: 1269 6550
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5000 6826 809
Size: 507 3684 1826 1802
Fragments arranged by size:
3684 1826 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2632 4189 4501
Size: 54 1425 50 275 18 802 1557 312
Cuts at: 4501 4636 4682 4696 5266 6155 7784 8
Size: 135 46 14 570 889 1629 43
Fragments arranged by size:
1629 1557 1425 889 802 570 312 275
135 54 50 46 43 18 14
EagI C'GGCC_G
Cuts at: 1537 1812 5266 1537
Size: 275 3454 4090
Fragments arranged by size:
4090 3454 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3278 4798 5749 1353
Size: 1925 1520 951 3423
Fragments arranged by size:
3423 1925 1520 951
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6406 6780 6926 387
Size: 1064 138 4817 374 146 1280
Fragments arranged by size:
4817 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 4383 5882 6479 7266 7534 1239
Size: 393 2751 1499 597 787 268 1524
Fragments arranged by size:
2751 1524 1499 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 4530 4530
Size: 7819
EcoRI G'AATT_C
Cuts at: 5128 5506 5128
Size: 378 7441
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4766 4766
Size: 7819
FspI TGC'GCA
Cuts at: 6325 6325
Size: 7819
HaeII r_GCGC'y
Cuts at: 1678 4960 6966 1678
Size: 3282 2006 2531
Fragments arranged by size:
3282 2531 2006
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3182 3214 4219 4251 4880
Size: 32 135 32 1610 32 1005 32 629
Cuts at: 4880 4912 6460 6492 6534 6566 1373
Size: 32 1548 32 42 32 2626
Fragments arranged by size:
2626 1610 1548 1005 629 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2380 2529 4453 4881 5005 5163
Size: 988 281 442 149 1924 428 124 158
Cuts at: 5163 5409 669
Size: 246 3079
Fragments arranged by size:
3079 1924 988 442 428 281 246 158
149 124
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7819
HpaI GTT'AAC
Cuts at: 4453 5409 4453
Size: 956 6863
KasI G'GCGC_C
Cuts at: 4956 4956
Size: 7819
KpnI G_GTAC'C
Cuts at: 5144 5144
Size: 7819
MfeI C'AATT_G
Cuts at: 3948 5418 3948
Size: 1470 6349
MluI A'CGCG_T
Cuts at: 7695 7695
Size: 7819
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2601 2770 3304 6933 7117 1305
Size: 1296 169 534 3629 184 2007
Fragments arranged by size:
3629 2007 1296 534 184 169
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4191 4503 4638 10
Size: 54 1425 343 2359 312 135 3191
Fragments arranged by size:
3191 2359 1425 343 312 135 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2166 2889 5777 6136 6295 518
Size: 648 1000 723 2888 359 159 2042
Fragments arranged by size:
2888 2042 1000 723 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2023 3976 5235 5912 7060 7305 1606
Size: 417 1953 1259 677 1148 245 2120
Fragments arranged by size:
2120 1953 1259 1148 677 417 245
NarI GG'CG_CC
Cuts at: 4957 4957
Size: 7819
NcoI C'CATG_G
Cuts at: 513 1065 2048 5085 513
Size: 552 983 3037 3247
Fragments arranged by size:
3247 3037 983 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7819
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7819
NotI GC'GGCC_GC
Cuts at: 5266 5266
Size: 7819
NspI r_CATG'y
Cuts at: 1132 1229 3223 4200 5177 6722 1132
Size: 97 1994 977 977 1545 2229
Fragments arranged by size:
2229 1994 1545 977 977 97
PasI CC'CwG_GG
Cuts at: 1348 3101 3278 1348
Size: 1753 177 5889
Fragments arranged by size:
5889 1753 177
PciI A'CATG_T
Cuts at: 3219 6718 3219
Size: 3499 4320
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3623 3875 1108
Size: 2515 252 5052
Fragments arranged by size:
5052 2515 252
PfoI T'CCnGG_A
Cuts at: 4848 4848
Size: 7819
PmeI GTTT'AAAC
Cuts at: 5123 5123
Size: 7819
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5888 5920 7401 7433 5888
Size: 32 1481 32 6274
Fragments arranged by size:
6274 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7819
PsiI TTA'TAA
Cuts at: 2979 5389 2979
Size: 2410 5409
PspOMI G'GGCC_C
Cuts at: 4530 4530
Size: 7819
PstI C_TGCA'G
Cuts at: 830 2022 2812 5171 830
Size: 1192 790 2359 3478
Fragments arranged by size:
3478 2359 1192 790
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7819
PvuII CAG'CTG
Cuts at: 3976 3976
Size: 7819
SalI G'TCGA_C
Cuts at: 1936 5161 1936
Size: 3225 4594
SapI GCTCTTCn'nnn_
Cuts at: 4798 4798
Size: 7819
SbfI CC_TGCA'GG
Cuts at: 5171 5171
Size: 7819
ScaI AGT'ACT
Cuts at: 2846 3874 4108 4494 6067 7779 2846
Size: 1028 234 386 1573 1712 2886
Fragments arranged by size:
2886 1712 1573 1028 386 234
SfcI C'TryA_G
Cuts at: 826 1045 2018 2808 3738 5167 6302 6983 7174
Size: 219 973 790 930 1429 1135 681 191
Cuts at: 7174 826
Size: 1471
Fragments arranged by size:
1471 1429 1135 973 930 790 681 219
191
SfoI GGC'GCC
Cuts at: 4958 4958
Size: 7819
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7819
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7819
SmaI CCC'GGG
Cuts at: 1872 5146 1872
Size: 3274 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3184 3431 4034 4946 5923 6824
Size: 197 926 1241 247 603 912 977 901
Cuts at: 6824 7086 820
Size: 262 1553
Fragments arranged by size:
1553 1241 977 926 912 901 603 262
247 197
SnaBI TAC'GTA
Cuts at: 493 5610 493
Size: 5117 2702
SpeI A'CTAG_T
Cuts at: 152 4109 4271 152
Size: 3957 162 3700
Fragments arranged by size:
3957 3700 162
SphI G_CATG'C
Cuts at: 1132 5177 1132
Size: 4045 3774
SspI AAT'ATT
Cuts at: 5 52 2178 2828 3700 4582 5743 5
Size: 47 2126 650 872 882 1161 2081
Fragments arranged by size:
2126 2081 1161 882 872 650 47
StuI AGG'CCT
Cuts at: 1517 4816 1517
Size: 3299 4520
StyI C'CwwG_G
Cuts at: 513 1065 2048 2134 2667 5085 5258 513
Size: 552 983 86 533 2418 173 3074
Fragments arranged by size:
3074 2418 983 552 533 173 86
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5846 5863 6016 6201 6646 325
Size: 1255 4266 17 153 185 445 1498
Fragments arranged by size:
4266 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2844 3263 3872
Size: 274 80 33 51 1443 867 419 609
Cuts at: 3872 4106 4492 6065 7777 96
Size: 234 386 1573 1712 138
Fragments arranged by size:
1712 1573 1443 867 609 419 386 274
234 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7779 7811 7779
Size: 32 7787
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7819
XbaI T'CTAG_A
Cuts at: 2602 5155 2602
Size: 2553 5266
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4426 4481 4846 4987 4426
Size: 55 365 141 7258
Fragments arranged by size:
7258 365 141 55
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7819
XmaI C'CCGG_G
Cuts at: 1870 5144 1870
Size: 3274 4545
XmnI GAAnn'nnTTC
Cuts at: 4650 4705 5948 4650
Size: 55 1243 6521
Fragments arranged by size:
6521 1243 55
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5627 276
Size: 53 83 186 936 4093 2468
Fragments arranged by size:
4093 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BfrBI BplI BsiWI Bsu36I
EcoICRI EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI
NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI
SacII SanDI SexAI SfiI SrfI SwaI