Restriction map for
pFN21AB3700
(Circular) (Six-base) MAPSORT of: pFN21AB3700.seq Check: 7076 from: 1 to: 6625
pFN21AB3700 6625 bp
With 182 enzymes: SgfI *
February 27, 2009 14:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3530 3530
Size: 6625
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4435 278
Size: 53 83 186 936 2899 2468
Fragments arranged by size:
2899 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3239 3968 1937
Size: 1302 729 4594
Fragments arranged by size:
4594 1302 729
Acc65I G'GTAC_C
Cuts at: 3946 3946
Size: 6625
AclI AA'CG_TT
Cuts at: 1563 4752 5125 1563
Size: 3189 373 3063
Fragments arranged by size:
3189 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3785 4688 6072 6340 1632
Size: 2153 903 1384 268 1917
Fragments arranged by size:
2153 1917 1384 903 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6428
AflIII A'CryG_T
Cuts at: 1203 2991 3568 5524 6501 1203
Size: 1788 577 1956 977 1327
Fragments arranged by size:
1956 1788 1327 977 577
AgeI A'CCGG_T
Cuts at: 5456 5456
Size: 6625
AhdI GACnn_n'nnGTC
Cuts at: 5354 5354
Size: 6625
AleI CACnn'nnGTG
Cuts at: 1166 2777 3473 1166
Size: 1611 696 4318
Fragments arranged by size:
4318 1611 696
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2093 1184
Size: 909 5716
AlwNI CAG_nnn'CTG
Cuts at: 2533 2717 3823 3881 4368 5940 2533
Size: 184 1106 58 487 1572 3218
Fragments arranged by size:
3218 1572 1106 487 184 58
ApaI G_GGCC'C
Cuts at: 2145 2886 2145
Size: 741 5884
ApaLI G'TGCA_C
Cuts at: 2666 3788 3890 4682 5838 2666
Size: 1122 102 792 1156 3453
Fragments arranged by size:
3453 1156 1122 792 102
ApoI r'AATT_y
Cuts at: 1493 3773 3934 4160 4312 6421 1493
Size: 2280 161 226 152 2109 1697
Fragments arranged by size:
2280 2109 1697 226 161 152
AseI AT'TA_AT
Cuts at: 160 1032 5179 160
Size: 872 4147 1606
Fragments arranged by size:
4147 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6625
AvaI C'yCGr_G
Cuts at: 1870 1943 2280 3395 3950 1870
Size: 73 337 1115 555 4545
Fragments arranged by size:
4545 1115 555 337 73
BamHI G'GATC_C
Cuts at: 3955 4322 3955
Size: 367 6258
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3086 3671 3946 5401 618
Size: 325 217 627 1299 585 275 1455 1842
Fragments arranged by size:
1842 1455 1299 627 585 325 275 217
BanII G_rGCy'C
Cuts at: 1391 2145 2592 2886 3513 3533 3944 1391
Size: 754 447 294 627 20 411 4072
Fragments arranged by size:
4072 754 627 447 411 294 20
BbeI G_GCGC'C
Cuts at: 3090 3090
Size: 6625
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3367 3457 928
Size: 971 1468 90 4096
Fragments arranged by size:
4096 1468 971 90
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2247 2281 4816 4850 1903
Size: 34 310 34 2535 34 3678
Fragments arranged by size:
3678 2535 310 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4517 5727 4517
Size: 1210 5415
BclI T'GATC_A
Cuts at: 1548 3030 3624 1548
Size: 1482 594 4549
Fragments arranged by size:
4549 1482 594
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2238 2513 2679
Size: 107 122 71 631 605 566 275 166
Cuts at: 2679 2735 3252 3444 5236 136
Size: 56 517 192 1792 1525
Fragments arranged by size:
1792 1525 631 605 566 517 275 192
166 122 107 71 56
BglII A'GATC_T
Cuts at: 6620 6620
Size: 6625
BlpI GC'TnA_GC
Cuts at: 3722 4042 3722
Size: 320 6305
Bme1580I G_kGCm'C
Cuts at: 1790 2135 2145 2670 2886 3480 3674 3792 3894
Size: 345 10 525 216 594 194 118 102
Cuts at: 3894 4686 5842 1790
Size: 792 1156 2573
Fragments arranged by size:
2573 1156 792 594 525 345 216 194
118 102 10
BmgBI CAC'GTC
Cuts at: 1318 2404 2992 3063 1318
Size: 1086 588 71 4880
Fragments arranged by size:
4880 1086 588 71
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4356 5314 442
Size: 416 53 83 399 24 2939 958 1753
Fragments arranged by size:
2939 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6625
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2908 3547 3800 5285 1239
Size: 1669 639 253 1485 2579
Fragments arranged by size:
2579 1669 1485 639 253
Bpu10I CC'TnA_GC
Cuts at: 3047 3047
Size: 6625
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3575 4750 5615 5913 3575
Size: 1175 865 298 4287
Fragments arranged by size:
4287 1175 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5288 882
Size: 617 3789 2219
Fragments arranged by size:
3789 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4416 493
Size: 711 3212 2702
Fragments arranged by size:
3212 2702 711
BsaBI GATnn'nnATC
Cuts at: 3215 6619 3215
Size: 3404 3221
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3087 4432 4814 5449
Size: 53 83 186 936 1554 1345 382 635
Cuts at: 5449 275
Size: 1451
Fragments arranged by size:
1554 1451 1345 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5058 5456 5730 5877 6288 5058
Size: 398 274 147 411 5395
Fragments arranged by size:
5395 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2276 2306 2441 2447 2471
Size: 30 165 30 873 30 135 6 24
Cuts at: 2471 2477 3633 3663 1178
Size: 6 1156 30 4140
Fragments arranged by size:
4140 1156 873 165 135 30 30 30
30 24 6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2918 3083 3314 1186
Size: 1732 165 231 4497
Fragments arranged by size:
4497 1732 231 165
BseYI C'CCAG_C
Cuts at: 3549 3863 5828 6324 3549
Size: 314 1965 496 3850
Fragments arranged by size:
3850 1965 496 314
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2207 2975 3683 1163
Size: 573 471 768 708 4105
Fragments arranged by size:
4105 768 708 573 471
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4075 4836 5864 1540
Size: 275 186 2074 761 1028 2301
Fragments arranged by size:
2301 2074 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2082 2670 3533 3792 3894 4686 4771 5842 2082
Size: 588 863 259 102 792 85 1071 2865
Fragments arranged by size:
2865 1071 863 792 588 259 102 85
BsmI GAATG_Cn'
Cuts at: 4136 4229 4136
Size: 93 6532
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2082 2135 2145 2592 2670 2886 3480
Size: 399 292 53 10 447 78 216 594
Cuts at: 3480 3513 3533 3674 3792 3894 3944 4686 4771
Size: 33 20 141 118 102 50 742 85
Cuts at: 4771 5842 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 594 447 399 292 216
141 118 102 85 78 53 50 33
20 10
BspEI T'CCGG_A
Cuts at: 6288 6288
Size: 6625
BspHI T'CATG_A
Cuts at: 4514 6244 4514
Size: 1730 4895
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2229 3530 3879 3980 844
Size: 1385 1301 349 101 3489
Fragments arranged by size:
3489 1385 1301 349 101
BsrBI CCG'CTC
Cuts at: 1394 1823 2869 2932 3654 4512 1394
Size: 429 1046 63 722 858 3507
Fragments arranged by size:
3507 1046 858 722 429 63
BsrDI GCAATG_nn'
Cuts at: 65 1248 2476 5120 5294 65
Size: 1183 1228 2644 174 1396
Fragments arranged by size:
2644 1396 1228 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2064 2418 2997 3344 3855 5269 5456
Size: 110 254 354 579 347 511 1414 187
Cuts at: 5456 1700
Size: 2869
Fragments arranged by size:
2869 1414 579 511 354 347 254 187
110
BsrGI T'GTAC_A
Cuts at: 96 3739 96
Size: 3643 2982
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6625
BssSI C'ACGA_G
Cuts at: 2011 4685 5697 2011
Size: 2674 1012 2939
Fragments arranged by size:
2939 2674 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3328 794
Size: 2534 4091
BstBI TT'CG_AA
Cuts at: 3180 5429 3180
Size: 2249 4376
BstEII G'GTnAC_C
Cuts at: 1184 3556 1184
Size: 2372 4253
BstYI r'GATC_y
Cuts at: 1972 3955 4322 4707 4724 6176 6262 6274 6620
Size: 1983 367 385 17 1452 86 12 346
Cuts at: 6620 1972
Size: 1977
Fragments arranged by size:
1983 1977 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2051 2051
Size: 6625
BtgI C'CryG_G
Cuts at: 513 1065 2800 3121 513
Size: 552 1735 321 4017
Fragments arranged by size:
4017 1735 552 321
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5886
Fragments arranged by size:
5886 683 56
BtsI GCAGTG_nn'
Cuts at: 2712 4141 4935 4955 2712
Size: 1429 794 20 4382
Fragments arranged by size:
4382 1429 794 20
ClaI AT'CG_AT
Cuts at: 1554 2412 1554
Size: 858 5767
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6590
DraI TTT'AAA
Cuts at: 3929 3929
Size: 6625
DraIII CAC_nnn'GTG
Cuts at: 3760 3760
Size: 6625
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5632 809
Size: 507 4316 1802
Fragments arranged by size:
4316 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3227 3270 3885
Size: 54 1425 50 275 18 1397 43 615
Cuts at: 3885 4072 4961 6590 8
Size: 187 889 1629 43
Fragments arranged by size:
1629 1425 1397 889 615 275 187 54
50 43 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4072 1537
Size: 275 2260 4090
Fragments arranged by size:
4090 2260 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2087 2102 4555 1353
Size: 734 15 2453 3423
Fragments arranged by size:
3423 2453 734 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2834 5212 5586 5732 387
Size: 1064 138 1245 2378 374 146 1280
Fragments arranged by size:
2378 1280 1245 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3531 3531
Size: 6625
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2908 3547 3785 3800 4688 5285 6072
Size: 393 1276 639 238 15 888 597 787
Cuts at: 6072 6340 1239
Size: 268 1524
Fragments arranged by size:
1524 1276 888 787 639 597 393 268
238 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2225 2603 2750 2225
Size: 378 147 6100
Fragments arranged by size:
6100 378 147
EcoO109I rG'GnC_Cy
Cuts at: 2089 2141 2182 2332 2530 2883 3106 3820 2089
Size: 52 41 150 198 353 223 714 4894
Fragments arranged by size:
4894 714 353 223 198 150 52 41
EcoRI G'AATT_C
Cuts at: 3934 4312 3934
Size: 378 6247
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2098 3169 2098
Size: 1071 5554
FspI TGC'GCA
Cuts at: 2783 2852 3383 5131 2783
Size: 69 531 1748 4277
Fragments arranged by size:
4277 1748 531 69
HaeII r_GCGC'y
Cuts at: 1678 2386 3090 5772 1678
Size: 708 704 2682 2531
Fragments arranged by size:
2682 2531 708 704
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3464 3496 3633 3665 5266
Size: 32 135 32 1892 32 137 32 1601
Cuts at: 5266 5298 5340 5372 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1892 1601 137 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2434 3969 4215 669
Size: 988 281 496 1535 246 3079
Fragments arranged by size:
3079 1535 988 496 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6625
HpaI GTT'AAC
Cuts at: 4215 4215
Size: 6625
KasI G'GCGC_C
Cuts at: 3086 3086
Size: 6625
KpnI G_GTAC'C
Cuts at: 3950 3950
Size: 6625
MfeI C'AATT_G
Cuts at: 4224 4224
Size: 6625
MluI A'CGCG_T
Cuts at: 6501 6501
Size: 6625
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2484 2814 3507 5739 5923 1305
Size: 1179 330 693 2232 184 2007
Fragments arranged by size:
2232 2007 1179 693 330 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3272 10
Size: 54 1425 343 1440 3363
Fragments arranged by size:
3363 1440 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2777 3473 4583 4942 5101 518
Size: 648 1611 696 1110 359 159 2042
Fragments arranged by size:
2042 1611 1110 696 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2717 3123 3226 3553 4041 4718 5866 6111
Size: 1111 406 103 327 488 677 1148 245
Cuts at: 6111 1606
Size: 2120
Fragments arranged by size:
2120 1148 1111 677 488 406 327 245
103
NaeI GCC'GGC
Cuts at: 2066 3346 3857 2066
Size: 1280 511 4834
Fragments arranged by size:
4834 1280 511
NarI GG'CG_CC
Cuts at: 3087 3087
Size: 6625
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6073
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6625
NgoMIV G'CCGG_C
Cuts at: 2064 3344 3855 2064
Size: 1280 511 4834
Fragments arranged by size:
4834 1280 511
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6625
NotI GC'GGCC_GC
Cuts at: 4072 4072
Size: 6625
NspI r_CATG'y
Cuts at: 1132 1229 2659 3322 3572 3983 5528 1132
Size: 97 1430 663 250 411 1545 2229
Fragments arranged by size:
2229 1545 1430 663 411 250 97
PasI CC'CwG_GG
Cuts at: 1348 2859 1348
Size: 1511 5114
PciI A'CATG_T
Cuts at: 3568 5524 3568
Size: 1956 4669
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2533 1108
Size: 1425 5200
PfoI T'CCnGG_A
Cuts at: 3840 3840
Size: 6625
PmeI GTTT'AAAC
Cuts at: 3929 3929
Size: 6625
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4694 4726 6207 6239 4694
Size: 32 1481 32 5080
Fragments arranged by size:
5080 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2089 2182 2332 3106 2089
Size: 93 150 774 5608
Fragments arranged by size:
5608 774 150 93
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6625
PsiI TTA'TAA
Cuts at: 4195 4195
Size: 6625
PspOMI G'GGCC_C
Cuts at: 2141 2882 2141
Size: 741 5884
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3155 3187 3296 3328 3155
Size: 32 109 32 6452
Fragments arranged by size:
6452 109 32 32
PstI C_TGCA'G
Cuts at: 830 3333 3977 830
Size: 2503 644 3478
Fragments arranged by size:
3478 2503 644
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6625
PvuII CAG'CTG
Cuts at: 2717 3553 2717
Size: 836 5789
SacI G_AGCT'C
Cuts at: 3533 3533
Size: 6625
SacII CC_GC'GG
Cuts at: 3124 3124
Size: 6625
SalI G'TCGA_C
Cuts at: 1936 3967 1936
Size: 2031 4594
SanDI GG'GwC_CC
Cuts at: 2332 2332
Size: 6625
SapI GCTCTTCn'nnn_
Cuts at: 2087 2087
Size: 6625
SbfI CC_TGCA'GG
Cuts at: 3977 3977
Size: 6625
ScaI AGT'ACT
Cuts at: 4873 6585 4873
Size: 1712 4913
SfcI C'TryA_G
Cuts at: 826 1045 3329 3973 5108 5789 5980 826
Size: 219 2284 644 1135 681 191 1471
Fragments arranged by size:
2284 1471 1135 681 644 219 191
SfoI GGC'GCC
Cuts at: 3088 3088
Size: 6625
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6625
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6625
SmaI CCC'GGG
Cuts at: 1872 2282 3952 1872
Size: 410 1670 4545
Fragments arranged by size:
4545 1670 410
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3590 4729 5630 5892 820
Size: 197 926 1647 1139 901 262 1553
Fragments arranged by size:
1647 1553 1139 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4416 493
Size: 3923 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6625
SphI G_CATG'C
Cuts at: 1132 3322 3983 1132
Size: 2190 661 3774
Fragments arranged by size:
3774 2190 661
SspI AAT'ATT
Cuts at: 5 52 4549 5
Size: 47 4497 2081
Fragments arranged by size:
4497 2081 47
StuI AGG'CCT
Cuts at: 1517 2501 1517
Size: 984 5641
StyI C'CwwG_G
Cuts at: 513 1065 2166 4064 513
Size: 552 1101 1898 3074
Fragments arranged by size:
3074 1898 1101 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2386 2618 2933 3861 4652 4669 4822
Size: 1255 806 232 315 928 791 17 153
Cuts at: 4822 5007 5452 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 928 806 791 445 315 232
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3171 3687 3739
Size: 274 80 33 51 1443 1194 516 52
Cuts at: 3739 4871 6583 96
Size: 1132 1712 138
Fragments arranged by size:
1712 1443 1194 1132 516 274 138 80
52 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2276 2308 2447 2479 6585 6617 2276
Size: 32 139 32 4106 32 2284
Fragments arranged by size:
4106 2284 139 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2405 3647 1316
Size: 1089 1242 4294
Fragments arranged by size:
4294 1242 1089
XbaI T'CTAG_A
Cuts at: 3961 3961
Size: 6625
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2534 3522 2534
Size: 988 5637
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6625
XmaI C'CCGG_G
Cuts at: 1870 2280 3950 1870
Size: 410 1670 4545
Fragments arranged by size:
4545 1670 410
XmnI GAAnn'nnTTC
Cuts at: 4754 4754
Size: 6625
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4433 276
Size: 53 83 186 936 2899 2468
Fragments arranged by size:
2899 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I EcoRV FseI FspAI NruI
NsiI PacI PmlI PspXI RsrII SexAI SfiI SrfI
SwaI