Restriction map for
pFN21AB3698
(Circular) (Six-base) MAPSORT of: pFN21AB3698.seq Check: 6221 from: 1 to: 5830
pFN21AB3698 5830 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2604 2604
Size: 5830
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3640 278
Size: 53 83 186 936 2104 2468
Fragments arranged by size:
2468 2104 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3173 1937
Size: 1236 4594
Acc65I G'GTAC_C
Cuts at: 3151 3151
Size: 5830
AclI AA'CG_TT
Cuts at: 1563 3957 4330 1563
Size: 2394 373 3063
Fragments arranged by size:
3063 2394 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2980 3092 3893 5277 5545 1632
Size: 1348 112 801 1384 268 1917
Fragments arranged by size:
1917 1384 1348 801 268 112
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5633
AflIII A'CryG_T
Cuts at: 1203 2424 4729 5706 1203
Size: 1221 2305 977 1327
Fragments arranged by size:
2305 1327 1221 977
AgeI A'CCGG_T
Cuts at: 4661 4661
Size: 5830
AhdI GACnn_n'nnGTC
Cuts at: 4559 4559
Size: 5830
AleI CACnn'nnGTG
Cuts at: 1166 2573 2588 1166
Size: 1407 15 4408
Fragments arranged by size:
4408 1407 15
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5830
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3098 3130 3098
Size: 32 5798
AlwNI CAG_nnn'CTG
Cuts at: 3573 5145 3573
Size: 1572 4258
ApaLI G'TGCA_C
Cuts at: 2392 2591 2846 3887 5043 2392
Size: 199 255 1041 1156 3179
Fragments arranged by size:
3179 1156 1041 255 199
ApoI r'AATT_y
Cuts at: 1493 3034 3139 3365 3517 5626 1493
Size: 1541 105 226 152 2109 1697
Fragments arranged by size:
2109 1697 1541 226 152 105
AseI AT'TA_AT
Cuts at: 160 1032 4384 160
Size: 872 3352 1606
Fragments arranged by size:
3352 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5830
AvaI C'yCGr_G
Cuts at: 1870 1943 2025 2090 2654 2984 3155 1870
Size: 73 82 65 564 330 171 4545
Fragments arranged by size:
4545 564 330 171 82 73 65
BamHI G'GATC_C
Cuts at: 3160 3527 3160
Size: 367 5463
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2054 2120 2575 2742 3151
Size: 325 217 627 267 66 455 167 409
Cuts at: 3151 4606 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 455 409 325 267 217
167 66
BanII G_rGCy'C
Cuts at: 1391 2097 2151 3149 1391
Size: 706 54 998 4072
Fragments arranged by size:
4072 998 706 54
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2431 2797 2827 928
Size: 971 532 366 30 3931
Fragments arranged by size:
3931 971 532 366 30
BbvCI CC'TCA_GC
Cuts at: 3008 3008
Size: 5830
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2770 2804 4021 4055 1903
Size: 34 833 34 1217 34 3678
Fragments arranged by size:
3678 1217 833 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3722 4932 3722
Size: 1210 4620
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5830
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2764 3074 4441
Size: 107 122 71 631 605 1092 310 1367
Cuts at: 4441 136
Size: 1525
Fragments arranged by size:
1525 1367 1092 631 605 310 122 107
71
BglII A'GATC_T
Cuts at: 5825 5825
Size: 5830
BlpI GC'TnA_GC
Cuts at: 2531 3247 2531
Size: 716 5114
Bme1580I G_kGCm'C
Cuts at: 1790 2123 2396 2580 2595 2682 2850 3891 5047
Size: 333 273 184 15 87 168 1041 1156
Cuts at: 5047 1790
Size: 2573
Fragments arranged by size:
2573 1156 1041 333 273 184 168 87
15
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5830
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3561 4519 442
Size: 416 53 83 399 24 2144 958 1753
Fragments arranged by size:
2144 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5830
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2060 4490 1239
Size: 821 2430 2579
Fragments arranged by size:
2579 2430 821
Bpu10I CC'TnA_GC
Cuts at: 3008 3008
Size: 5830
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3955 4820 5118 3955
Size: 865 298 4667
Fragments arranged by size:
4667 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2984 4493 882
Size: 617 1485 1509 2219
Fragments arranged by size:
2219 1509 1485 617
BsaAI yAC'GTr
Cuts at: 493 1204 2170 3621 493
Size: 711 966 1451 2702
Fragments arranged by size:
2702 1451 966 711
BsaBI GATnn'nnATC
Cuts at: 2279 5824 2279
Size: 3545 2285
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3637 4019 4654 275
Size: 53 83 186 936 2104 382 635 1451
Fragments arranged by size:
2104 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4263 4661 4935 5082 5493 4263
Size: 398 274 147 411 4600
Fragments arranged by size:
4600 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2069 2099 2633 2663 1178
Size: 30 165 30 666 30 534 30 4345
Fragments arranged by size:
4345 666 534 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5830
BseYI C'CCAG_C
Cuts at: 2665 5033 5529 2665
Size: 2368 496 2966
Fragments arranged by size:
2966 2368 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2345 2666 1163
Size: 573 609 321 4327
Fragments arranged by size:
4327 609 573 321
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2467 3280 4041 5069 1540
Size: 275 186 466 813 761 1028 2301
Fragments arranged by size:
2301 1028 813 761 466 275 186
BsiHKAI G_wGCw'C
Cuts at: 2097 2151 2160 2396 2595 2850 3891 3976 5047
Size: 54 9 236 199 255 1041 85 1071
Cuts at: 5047 2097
Size: 2880
Fragments arranged by size:
2880 1071 1041 255 236 199 85 54
9
BsmI GAATG_Cn'
Cuts at: 3341 3434 3341
Size: 93 5737
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2097 2123 2151 2160 2396 2580 2595
Size: 399 307 26 28 9 236 184 15
Cuts at: 2595 2682 2850 3149 3891 3976 5047 1391
Size: 87 168 299 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 307 299 236 184
168 87 85 28 26 15 9
BspEI T'CCGG_A
Cuts at: 5493 5493
Size: 5830
BspHI T'CATG_A
Cuts at: 3719 5449 3719
Size: 1730 4100
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2367 2407 2604 3185 844
Size: 1523 40 197 581 3489
Fragments arranged by size:
3489 1523 581 197 40
BsrBI CCG'CTC
Cuts at: 1394 1823 2113 2368 2879 3717 1394
Size: 429 290 255 511 838 3507
Fragments arranged by size:
3507 838 511 429 290 255
BsrDI GCAATG_nn'
Cuts at: 65 1248 4325 4499 65
Size: 1183 3077 174 1396
Fragments arranged by size:
3077 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2739 3040 4474 4661 1700
Size: 110 929 301 1434 187 2869
Fragments arranged by size:
2869 1434 929 301 187 110
BsrGI T'GTAC_A
Cuts at: 96 2427 3015 96
Size: 2331 588 2911
Fragments arranged by size:
2911 2331 588
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5830
BssSI C'ACGA_G
Cuts at: 2188 2363 3890 4902 2188
Size: 175 1527 1012 3116
Fragments arranged by size:
3116 1527 1012 175
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2854 794
Size: 2060 3770
BstBI TT'CG_AA
Cuts at: 4634 4634
Size: 5830
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5830
BstYI r'GATC_y
Cuts at: 1972 3160 3527 3912 3929 5381 5467 5479 5825
Size: 1188 367 385 17 1452 86 12 346
Cuts at: 5825 1972
Size: 1977
Fragments arranged by size:
1977 1452 1188 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2746 3126 513
Size: 552 939 742 380 3217
Fragments arranged by size:
3217 939 742 552 380
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2365 2458 487
Size: 683 56 1139 93 3859
Fragments arranged by size:
3859 1139 683 93 56
BtsI GCAGTG_nn'
Cuts at: 2654 3127 3346 4140 4160 2654
Size: 473 219 794 20 4324
Fragments arranged by size:
4324 794 473 219 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5830
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5795
DraI TTT'AAA
Cuts at: 3134 3134
Size: 5830
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2384 4837 809
Size: 507 1068 2453 1802
Fragments arranged by size:
2453 1802 1068 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2130 2331 2481
Size: 54 1425 50 275 18 300 201 150
Cuts at: 2481 3277 4166 5795 8
Size: 796 889 1629 43
Fragments arranged by size:
1629 1425 889 796 300 275 201 150
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3277 1537
Size: 275 1465 4090
Fragments arranged by size:
4090 1465 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2216 2414 3760 1353
Size: 863 198 1346 3423
Fragments arranged by size:
3423 1346 863 198
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2330 2888 4417 4791 4937 387
Size: 1064 138 741 558 1529 374 146 1280
Fragments arranged by size:
1529 1280 1064 741 558 374 146 138
EcoICRI GAG'CTC
Cuts at: 2095 2149 2095
Size: 54 5776
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2060 2980 3092 3893 4490 5277 5545
Size: 393 428 920 112 801 597 787 268
Cuts at: 5545 1239
Size: 1524
Fragments arranged by size:
1524 920 801 787 597 428 393 268
112
EcoNI CCTnn'n_nnAGG
Cuts at: 2345 2345
Size: 5830
EcoRI G'AATT_C
Cuts at: 3139 3517 3139
Size: 378 5452
FspI TGC'GCA
Cuts at: 4336 4336
Size: 5830
HaeII r_GCGC'y
Cuts at: 1678 2777 4977 1678
Size: 1099 2200 2531
Fragments arranged by size:
2531 2200 1099
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4471 4503 4545 4577 1373
Size: 32 135 32 2899 32 42 32 2626
Fragments arranged by size:
2899 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2422 2932 3174 3420 669
Size: 988 281 484 510 242 246 3079
Fragments arranged by size:
3079 988 510 484 281 246 242
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5830
HpaI GTT'AAC
Cuts at: 3420 3420
Size: 5830
KpnI G_GTAC'C
Cuts at: 3155 3155
Size: 5830
MfeI C'AATT_G
Cuts at: 3429 3429
Size: 5830
MluI A'CGCG_T
Cuts at: 5706 5706
Size: 5830
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2973 3084 4944 5128 1305
Size: 1668 111 1860 184 2007
Fragments arranged by size:
2007 1860 1668 184 111
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2132 2483 10
Size: 54 1425 343 300 351 3357
Fragments arranged by size:
3357 1425 351 343 300 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2327 2348 2399 2453 2549 2573
Size: 648 843 318 21 51 54 96 24
Cuts at: 2573 2588 2843 3788 4147 4306 518
Size: 15 255 945 359 159 2042
Fragments arranged by size:
2042 945 843 648 359 318 255 159
96 54 51 24 21 15
MspA1I CmG'CkG
Cuts at: 1606 2860 2955 3246 3923 5071 5316 1606
Size: 1254 95 291 677 1148 245 2120
Fragments arranged by size:
2120 1254 1148 677 291 245 95
NaeI GCC'GGC
Cuts at: 3042 3042
Size: 5830
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4339
Fragments arranged by size:
4339 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5830
NgoMIV G'CCGG_C
Cuts at: 3040 3040
Size: 5830
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5830
NotI GC'GGCC_GC
Cuts at: 3277 3277
Size: 5830
NspI r_CATG'y
Cuts at: 1132 1229 2428 3188 4733 1132
Size: 97 1199 760 1545 2229
Fragments arranged by size:
2229 1545 1199 760 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5830
PciI A'CATG_T
Cuts at: 2424 4729 2424
Size: 2305 3525
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5830
PfoI T'CCnGG_A
Cuts at: 2637 2637
Size: 5830
PmeI GTTT'AAAC
Cuts at: 3134 3134
Size: 5830
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3899 3931 5412 5444 3899
Size: 32 1481 32 4285
Fragments arranged by size:
4285 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5830
PsiI TTA'TAA
Cuts at: 3400 3400
Size: 5830
PspXI vC'TCGA_Gb
Cuts at: 2654 2654
Size: 5830
PstI C_TGCA'G
Cuts at: 830 2418 2501 2601 2859 3182 830
Size: 1588 83 100 258 323 3478
Fragments arranged by size:
3478 1588 323 258 100 83
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5830
PvuII CAG'CTG
Cuts at: 2955 2955
Size: 5830
SacI G_AGCT'C
Cuts at: 2097 2151 2097
Size: 54 5776
SalI G'TCGA_C
Cuts at: 1936 3172 1936
Size: 1236 4594
SapI GCTCTTCn'nnn_
Cuts at: 2414 2414
Size: 5830
SbfI CC_TGCA'GG
Cuts at: 2418 3182 2418
Size: 764 5066
ScaI AGT'ACT
Cuts at: 4078 5790 4078
Size: 1712 4118
SfcI C'TryA_G
Cuts at: 826 1045 2414 2497 2597 2855 2974 3178 4313
Size: 219 1369 83 100 258 119 204 1135
Cuts at: 4313 4994 5185 826
Size: 681 191 1471
Fragments arranged by size:
1471 1369 1135 681 258 219 204 191
119 100 83
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5830
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5830
SmaI CCC'GGG
Cuts at: 1872 2027 3157 1872
Size: 155 1130 4545
Fragments arranged by size:
4545 1130 155
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2654 3934 4835 5097 820
Size: 197 926 711 1280 901 262 1553
Fragments arranged by size:
1553 1280 926 901 711 262 197
SnaBI TAC'GTA
Cuts at: 493 3621 493
Size: 3128 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5830
SphI G_CATG'C
Cuts at: 1132 3188 1132
Size: 2056 3774
SspI AAT'ATT
Cuts at: 5 52 3754 5
Size: 47 3702 2081
Fragments arranged by size:
3702 2081 47
StuI AGG'CCT
Cuts at: 1517 2615 1517
Size: 1098 4732
StyI C'CwwG_G
Cuts at: 513 1065 2004 3269 513
Size: 552 939 1265 3074
Fragments arranged by size:
3074 1265 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3857 3874 4027 4212 4657 325
Size: 1255 2277 17 153 185 445 1498
Fragments arranged by size:
2277 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2292 2427 3015
Size: 274 80 33 51 1443 315 135 588
Cuts at: 3015 4076 5788 96
Size: 1061 1712 138
Fragments arranged by size:
1712 1443 1061 588 315 274 138 135
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2633 2665 5790 5822 2633
Size: 32 3125 32 2641
Fragments arranged by size:
3125 2641 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5830
XbaI T'CTAG_A
Cuts at: 3166 3166
Size: 5830
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2224 2224
Size: 5830
XhoI C'TCGA_G
Cuts at: 1943 2654 1943
Size: 711 5119
XmaI C'CCGG_G
Cuts at: 1870 2025 3155 1870
Size: 155 1130 4545
Fragments arranged by size:
4545 1130 155
XmnI GAAnn'nnTTC
Cuts at: 2834 3053 3959 2834
Size: 219 906 4705
Fragments arranged by size:
4705 906 219
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3638 276
Size: 53 83 186 936 2104 2468
Fragments arranged by size:
2468 2104 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PspXI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoO109I EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PmlI PpuMI PspOMI PsrI RsrII SacII SanDI SexAI
SfiI SfoI SrfI SwaI