Restriction map for
pFN21AB3690
(Circular) (Six-base) MAPSORT of: pFN21AB3690.seq Check: 1641 from: 1 to: 5977
pFN21AB3690 5977 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3787 278
Size: 53 83 186 936 2251 2468
Fragments arranged by size:
2468 2251 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2940 3320 1937
Size: 1003 380 4594
Fragments arranged by size:
4594 1003 380
Acc65I G'GTAC_C
Cuts at: 3298 3298
Size: 5977
AclI AA'CG_TT
Cuts at: 1563 2077 4104 4477 1563
Size: 514 2027 373 3063
Fragments arranged by size:
3063 2027 514 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2519 2744 3117 4040 5424 5692 1632
Size: 887 225 373 923 1384 268 1917
Fragments arranged by size:
1917 1384 923 887 373 268 225
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5780
AflIII A'CryG_T
Cuts at: 1203 4876 5853 1203
Size: 3673 977 1327
Fragments arranged by size:
3673 1327 977
AgeI A'CCGG_T
Cuts at: 4808 4808
Size: 5977
AhdI GACnn_n'nnGTC
Cuts at: 2629 4706 2629
Size: 2077 3900
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5977
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5977
AlwNI CAG_nnn'CTG
Cuts at: 2089 2855 3217 3720 5292 2089
Size: 766 362 503 1572 2774
Fragments arranged by size:
2774 1572 766 503 362
ApaI G_GGCC'C
Cuts at: 2113 2113
Size: 5977
ApaLI G'TGCA_C
Cuts at: 2533 4034 5190 2533
Size: 1501 1156 3320
Fragments arranged by size:
3320 1501 1156
ApoI r'AATT_y
Cuts at: 1493 2303 2813 3012 3286 3512 3664 5773 1493
Size: 810 510 199 274 226 152 2109 1697
Fragments arranged by size:
2109 1697 810 510 274 226 199 152
AseI AT'TA_AT
Cuts at: 160 1032 2333 4531 160
Size: 872 1301 2198 1606
Fragments arranged by size:
2198 1606 1301 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5977
AvaI C'yCGr_G
Cuts at: 1870 1943 2680 3302 1870
Size: 73 737 622 4545
Fragments arranged by size:
4545 737 622 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2783 2816 2783
Size: 33 5944
BamHI G'GATC_C
Cuts at: 3307 3674 3307
Size: 367 5610
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2581 3298 4753 618
Size: 325 217 627 794 717 1455 1842
Fragments arranged by size:
1842 1455 794 717 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2113 3296 1391
Size: 722 1183 4072
Fragments arranged by size:
4072 1183 722
BbeI G_GCGC'C
Cuts at: 2585 2585
Size: 5977
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3165 928
Size: 971 1266 3740
Fragments arranged by size:
3740 1266 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4168 4202 1903
Size: 34 2231 34 3678
Fragments arranged by size:
3678 2231 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2811 3869 5079 2811
Size: 1058 1210 3709
Fragments arranged by size:
3709 1210 1058
BclI T'GATC_A
Cuts at: 1548 2698 1548
Size: 1150 4827
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4588 136
Size: 107 122 71 631 605 2916 1525
Fragments arranged by size:
2916 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2890 5972 2890
Size: 3082 2895
BlpI GC'TnA_GC
Cuts at: 3394 3394
Size: 5977
Bme1580I G_kGCm'C
Cuts at: 1790 2113 2537 4038 5194 1790
Size: 323 424 1501 1156 2573
Fragments arranged by size:
2573 1501 1156 424 323
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5977
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3019 3708 4666
Size: 416 53 83 399 24 1602 689 958
Cuts at: 4666 442
Size: 1753
Fragments arranged by size:
1753 1602 958 689 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5977
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4637 1239
Size: 3398 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4102 4967 5265 4102
Size: 865 298 4814
Fragments arranged by size:
4814 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4640 882
Size: 617 3141 2219
Fragments arranged by size:
3141 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2560 3768 493
Size: 711 1356 1208 2702
Fragments arranged by size:
2702 1356 1208 711
BsaBI GATnn'nnATC
Cuts at: 5971 5971
Size: 5977
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2582 3784 4166 4801
Size: 53 83 186 936 1049 1202 382 635
Cuts at: 4801 275
Size: 1451
Fragments arranged by size:
1451 1202 1049 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4410 4808 5082 5229 5640 4410
Size: 398 274 147 411 4747
Fragments arranged by size:
4747 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5752
Fragments arranged by size:
5752 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5977
BseYI C'CCAG_C
Cuts at: 2725 5180 5676 2725
Size: 2455 496 3026
Fragments arranged by size:
3026 2455 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2476 1163
Size: 573 740 4664
Fragments arranged by size:
4664 740 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3427 4188 5216 1540
Size: 275 186 1426 761 1028 2301
Fragments arranged by size:
2301 1426 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2537 4038 4123 5194 2537
Size: 1501 85 1071 3320
Fragments arranged by size:
3320 1501 1071 85
BsmI GAATG_Cn'
Cuts at: 2431 3488 3581 2431
Size: 1057 93 4827
Fragments arranged by size:
4827 1057 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2113 2537 3296 4038 4123 5194 1391
Size: 399 323 424 759 742 85 1071 2174
Fragments arranged by size:
2174 1071 759 742 424 399 323 85
BspEI T'CCGG_A
Cuts at: 5640 5640
Size: 5977
BspHI T'CATG_A
Cuts at: 2377 2544 2748 3866 5596 2377
Size: 167 204 1118 1730 2758
Fragments arranged by size:
2758 1730 1118 204 167
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3332 844
Size: 2488 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3864 1394
Size: 429 2041 3507
Fragments arranged by size:
3507 2041 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4472 4646 65
Size: 1183 3224 174 1396
Fragments arranged by size:
3224 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4621 4808 1700
Size: 110 2811 187 2869
Fragments arranged by size:
2869 2811 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5977
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5977
BssSI C'ACGA_G
Cuts at: 4037 5049 4037
Size: 1012 4965
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5977
BstBI TT'CG_AA
Cuts at: 4781 4781
Size: 5977
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5977
BstYI r'GATC_y
Cuts at: 1972 2502 2890 2931 3307 3674 4059 4076 5528
Size: 530 388 41 376 367 385 17 1452
Cuts at: 5528 5614 5626 5972 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 530 388 385 376 367 346
86 41 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5425
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5238
Fragments arranged by size:
5238 683 56
BtsI GCAGTG_nn'
Cuts at: 3493 4287 4307 3493
Size: 794 20 5163
Fragments arranged by size:
5163 794 20
ClaI AT'CG_AT
Cuts at: 1554 3179 1554
Size: 1625 4352
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5942
DraI TTT'AAA
Cuts at: 3281 3281
Size: 5977
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4984 809
Size: 507 3668 1802
Fragments arranged by size:
3668 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3424 4313 5942
Size: 54 1425 50 275 18 1594 889 1629
Cuts at: 5942 8
Size: 43
Fragments arranged by size:
1629 1594 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3424 1537
Size: 275 1612 4090
Fragments arranged by size:
4090 1612 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2680 3907 1353
Size: 1327 1227 3423
Fragments arranged by size:
3423 1327 1227
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4564 4938 5084 387
Size: 1064 138 2975 374 146 1280
Fragments arranged by size:
2975 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2519 2744 3117 4040 4637 5424 5692
Size: 393 887 225 373 923 597 787 268
Cuts at: 5692 1239
Size: 1524
Fragments arranged by size:
1524 923 887 787 597 393 373 268
225
EcoO109I rG'GnC_Cy
Cuts at: 2109 3257 2109
Size: 1148 4829
EcoRI G'AATT_C
Cuts at: 3286 3664 3286
Size: 378 5599
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2199 2199
Size: 5977
FspI TGC'GCA
Cuts at: 4483 4483
Size: 5977
HaeII r_GCGC'y
Cuts at: 1678 2585 5124 1678
Size: 907 2539 2531
Fragments arranged by size:
2539 2531 907
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2200 2232 2615 2647 2882
Size: 32 135 32 628 32 383 32 235
Cuts at: 2882 2914 3179 3211 4618 4650 4692 4724 1373
Size: 32 265 32 1407 32 42 32 2626
Fragments arranged by size:
2626 1407 628 383 265 235 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3321 3567 669
Size: 988 281 1383 246 3079
Fragments arranged by size:
3079 1383 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5977
HpaI GTT'AAC
Cuts at: 3567 3567
Size: 5977
KasI G'GCGC_C
Cuts at: 2581 2581
Size: 5977
KpnI G_GTAC'C
Cuts at: 3302 3302
Size: 5977
MfeI C'AATT_G
Cuts at: 2097 3048 3576 2097
Size: 951 528 4498
Fragments arranged by size:
4498 951 528
MluI A'CGCG_T
Cuts at: 5853 5853
Size: 5977
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3009 5091 5275 1305
Size: 1704 2082 184 2007
Fragments arranged by size:
2082 2007 1704 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4155
Fragments arranged by size:
4155 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2477 3935 4294 4453 518
Size: 648 1311 1458 359 159 2042
Fragments arranged by size:
2042 1458 1311 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2089 2438 2725 3393 4070 5218 5463 1606
Size: 483 349 287 668 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 668 483 349 287 245
NarI GG'CG_CC
Cuts at: 2582 2582
Size: 5977
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5425
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5977
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5977
NotI GC'GGCC_GC
Cuts at: 3424 3424
Size: 5977
NspI r_CATG'y
Cuts at: 1132 1229 3047 3335 4880 1132
Size: 97 1818 288 1545 2229
Fragments arranged by size:
2229 1818 1545 288 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5977
PciI A'CATG_T
Cuts at: 4876 4876
Size: 5977
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5977
PfoI T'CCnGG_A
Cuts at: 2803 2803
Size: 5977
PmeI GTTT'AAAC
Cuts at: 3281 3281
Size: 5977
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2214 2246 4046 4078 5559 5591 2214
Size: 32 1800 32 1481 32 2600
Fragments arranged by size:
2600 1800 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3257 3257
Size: 5977
PshAI GACnn'nnGTC
Cuts at: 1613 3237 1613
Size: 1624 4353
PsiI TTA'TAA
Cuts at: 3547 3547
Size: 5977
PspOMI G'GGCC_C
Cuts at: 2109 2109
Size: 5977
PstI C_TGCA'G
Cuts at: 830 3329 830
Size: 2499 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5977
PvuII CAG'CTG
Cuts at: 2089 2438 2725 2089
Size: 349 287 5341
Fragments arranged by size:
5341 349 287
SalI G'TCGA_C
Cuts at: 1936 3319 1936
Size: 1383 4594
SanDI GG'GwC_CC
Cuts at: 3257 3257
Size: 5977
SbfI CC_TGCA'GG
Cuts at: 3329 3329
Size: 5977
ScaI AGT'ACT
Cuts at: 4225 5937 4225
Size: 1712 4265
SfcI C'TryA_G
Cuts at: 826 1045 2937 3325 4460 5141 5332 826
Size: 219 1892 388 1135 681 191 1471
Fragments arranged by size:
1892 1471 1135 681 388 219 191
SfoI GGC'GCC
Cuts at: 2583 2583
Size: 5977
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5977
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5977
SmaI CCC'GGG
Cuts at: 1872 2682 3304 1872
Size: 810 622 4545
Fragments arranged by size:
4545 810 622
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4081 4982 5244 820
Size: 197 926 2138 901 262 1553
Fragments arranged by size:
2138 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3768 493
Size: 3275 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5977
SphI G_CATG'C
Cuts at: 1132 3335 1132
Size: 2203 3774
SspI AAT'ATT
Cuts at: 5 52 3901 5
Size: 47 3849 2081
Fragments arranged by size:
3849 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5977
StyI C'CwwG_G
Cuts at: 513 1065 2113 3416 513
Size: 552 1048 1303 3074
Fragments arranged by size:
3074 1303 1048 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2973 4004 4021 4174 4359 4804 325
Size: 1255 1393 1031 17 153 185 445 1498
Fragments arranged by size:
1498 1393 1255 1031 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4223 5935 96
Size: 274 80 33 51 1443 2246 1712 138
Fragments arranged by size:
2246 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2110 2142 5937 5969 2110
Size: 32 3795 32 2118
Fragments arranged by size:
3795 2118 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5977
XbaI T'CTAG_A
Cuts at: 2296 3313 2296
Size: 1017 4960
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2411 2411
Size: 5977
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5977
XmaI C'CCGG_G
Cuts at: 1870 2680 3302 1870
Size: 810 622 4545
Fragments arranged by size:
4545 810 622
XmnI GAAnn'nnTTC
Cuts at: 2304 2826 4106 2304
Size: 522 1280 4175
Fragments arranged by size:
4175 1280 522
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3785 276
Size: 53 83 186 936 2251 2468
Fragments arranged by size:
2468 2251 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI
PacI PmlI PspXI PsrI RsrII SacI SacII SapI
SexAI SfiI SrfI SwaI