Restriction map for
pFN21AB3680
(Circular) (Six-base) MAPSORT of: pFN21AB3680.seq Check: 6104 from: 1 to: 7558
pFN21AB3680 7558 bp
With 182 enzymes: SgfI *
February 27, 2009 14:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5368 278
Size: 53 83 186 936 3832 2468
Fragments arranged by size:
3832 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4901 1937
Size: 2964 4594
Acc65I G'GTAC_C
Cuts at: 4879 4879
Size: 7558
AclI AA'CG_TT
Cuts at: 1563 5685 6058 1563
Size: 4122 373 3063
Fragments arranged by size:
4122 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2493 3537 4219 4731 5621 7005 7273 1632
Size: 861 1044 682 512 890 1384 268 1917
Fragments arranged by size:
1917 1384 1044 890 861 682 512 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7361
AflIII A'CryG_T
Cuts at: 1203 6457 7434 1203
Size: 5254 977 1327
Fragments arranged by size:
5254 1327 977
AgeI A'CCGG_T
Cuts at: 6389 6389
Size: 7558
AhdI GACnn_n'nnGTC
Cuts at: 6287 6287
Size: 7558
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7558
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2861 1184
Size: 1677 5881
AlwNI CAG_nnn'CTG
Cuts at: 2159 2498 2720 3586 5301 6873 2159
Size: 339 222 866 1715 1572 2844
Fragments arranged by size:
2844 1715 1572 866 339 222
ApaLI G'TGCA_C
Cuts at: 5615 6771 5615
Size: 1156 6402
ApoI r'AATT_y
Cuts at: 1493 4353 4535 4867 5093 5245 7354 1493
Size: 2860 182 332 226 152 2109 1697
Fragments arranged by size:
2860 2109 1697 332 226 182 152
AseI AT'TA_AT
Cuts at: 160 1032 6112 160
Size: 872 5080 1606
Fragments arranged by size:
5080 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7558
AvaI C'yCGr_G
Cuts at: 1870 1943 2654 4883 1870
Size: 73 711 2229 4545
Fragments arranged by size:
4545 2229 711 73
BamHI G'GATC_C
Cuts at: 3262 4888 5255 3262
Size: 1626 367 5565
Fragments arranged by size:
5565 1626 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4879 6334 618
Size: 325 217 627 3092 1455 1842
Fragments arranged by size:
3092 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2826 3735 4775 4877 1391
Size: 1435 909 1040 102 4072
Fragments arranged by size:
4072 1435 1040 909 102
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3953 4408 4703 928
Size: 971 2054 455 295 3783
Fragments arranged by size:
3783 2054 971 455 295
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5749 5783 1903
Size: 34 3812 34 3678
Fragments arranged by size:
3812 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5450 6660 5450
Size: 1210 6348
BclI T'GATC_A
Cuts at: 1548 3309 3508 1548
Size: 1761 199 5598
Fragments arranged by size:
5598 1761 199
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6169 136
Size: 107 122 71 631 605 4497 1525
Fragments arranged by size:
4497 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3406 7553 3406
Size: 4147 3411
BlpI GC'TnA_GC
Cuts at: 2036 2310 4975 2036
Size: 274 2665 4619
Fragments arranged by size:
4619 2665 274
Bme1580I G_kGCm'C
Cuts at: 1790 5619 6775 1790
Size: 3829 1156 2573
Fragments arranged by size:
3829 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7558
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2197 2669 2741
Size: 416 53 83 399 24 780 472 72
Cuts at: 2741 3729 3875 5289 6247 442
Size: 988 146 1414 958 1753
Fragments arranged by size:
1753 1414 988 958 780 472 416 399
146 83 72 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7558
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2656 2656
Size: 7558
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2154 2697 2945 3084 3201 3822 3903 6218
Size: 915 543 248 139 117 621 81 2315
Cuts at: 6218 1239
Size: 2579
Fragments arranged by size:
2579 2315 915 621 543 248 139 117
81
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5683 6548 6846 5683
Size: 865 298 6395
Fragments arranged by size:
6395 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2216 6221 882
Size: 617 717 4005 2219
Fragments arranged by size:
4005 2219 717 617
BsaAI yAC'GTr
Cuts at: 493 1204 5349 493
Size: 711 4145 2702
Fragments arranged by size:
4145 2702 711
BsaBI GATnn'nnATC
Cuts at: 3510 4602 7552 3510
Size: 1092 2950 3516
Fragments arranged by size:
3516 2950 1092
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4819 5365 5747 6382
Size: 53 83 186 936 3286 546 382 635
Cuts at: 6382 275
Size: 1451
Fragments arranged by size:
3286 1451 936 635 546 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5991 6389 6663 6810 7221 5991
Size: 398 274 147 411 6328
Fragments arranged by size:
6328 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2253 2283 2695 2725 3876
Size: 30 165 30 850 30 412 30 1151
Cuts at: 3876 3906 1178
Size: 30 4830
Fragments arranged by size:
4830 1151 850 412 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2480 4130 4166 1186
Size: 1294 1650 36 4578
Fragments arranged by size:
4578 1650 1294 36
BseYI C'CCAG_C
Cuts at: 3380 4286 6761 7257 3380
Size: 906 2475 496 3681
Fragments arranged by size:
3681 2475 906 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2706 3630 3759 4487 1163
Size: 573 970 924 129 728 4234
Fragments arranged by size:
4234 970 924 728 573 129
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5008 5769 6797 1540
Size: 275 186 3007 761 1028 2301
Fragments arranged by size:
3007 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2826 3016 5619 5704 6775 2826
Size: 190 2603 85 1071 3609
Fragments arranged by size:
3609 2603 1071 190 85
BsmI GAATG_Cn'
Cuts at: 4573 5069 5162 4573
Size: 496 93 6969
Fragments arranged by size:
6969 496 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2826 3016 3735 4775 4877 5619 5704
Size: 399 1036 190 719 1040 102 742 85
Cuts at: 5704 6775 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 1040 1036 742 719 399 190
102 85
BspEI T'CCGG_A
Cuts at: 7221 7221
Size: 7558
BspHI T'CATG_A
Cuts at: 2097 3153 5447 7177 2097
Size: 1056 2294 1730 2478
Fragments arranged by size:
2478 2294 1730 1056
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3766 4913 844
Size: 2922 1147 3489
Fragments arranged by size:
3489 2922 1147
BsrBI CCG'CTC
Cuts at: 1394 1823 3622 5445 1394
Size: 429 1799 1823 3507
Fragments arranged by size:
3507 1823 1799 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2436 2437 3480 6053 6227 65
Size: 1183 1188 1 1043 2573 174 1396
Fragments arranged by size:
2573 1396 1188 1183 1043 174 1
BsrFI r'CCGG_y
Cuts at: 1700 1810 6202 6389 1700
Size: 110 4392 187 2869
Fragments arranged by size:
4392 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4578 96
Size: 4482 3076
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7558
BssSI C'ACGA_G
Cuts at: 5618 6630 5618
Size: 1012 6546
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3011 3905 794
Size: 2217 894 4447
Fragments arranged by size:
4447 2217 894
BstBI TT'CG_AA
Cuts at: 6362 6362
Size: 7558
BstEII G'GTnAC_C
Cuts at: 1184 2541 2647 1184
Size: 1357 106 6095
Fragments arranged by size:
6095 1357 106
BstXI CCAn_nnnn'nTGG
Cuts at: 2554 3823 2554
Size: 1269 6289
BstYI r'GATC_y
Cuts at: 1972 3262 3406 4006 4644 4888 5255 5640 5657
Size: 1290 144 600 638 244 367 385 17
Cuts at: 5657 7109 7195 7207 7553 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1290 638 600 385 367 346
244 144 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4158 4158
Size: 7558
BtgI C'CryG_G
Cuts at: 513 1065 2004 2057 3246 3285 513
Size: 552 939 53 1189 39 4786
Fragments arranged by size:
4786 1189 939 552 53 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3936 487
Size: 683 56 2710 4109
Fragments arranged by size:
4109 2710 683 56
BtsI GCAGTG_nn'
Cuts at: 3301 3543 4253 4620 5074 5868 5888 3301
Size: 242 710 367 454 794 20 4971
Fragments arranged by size:
4971 794 710 454 367 242 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7558
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3049 3084 529
Size: 35 2485 35 5003
Fragments arranged by size:
5003 2485 35 35
DraI TTT'AAA
Cuts at: 4862 4862
Size: 7558
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4598 6565 809
Size: 507 3282 1967 1802
Fragments arranged by size:
3282 1967 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2161 2896 4140
Size: 54 1425 50 275 18 331 735 1244
Cuts at: 4140 4659 5005 5894 7523 8
Size: 519 346 889 1629 43
Fragments arranged by size:
1629 1425 1244 889 735 519 346 331
275 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5005 1537
Size: 275 3193 4090
Fragments arranged by size:
4090 3193 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2457 2625 3324 3444 4158 5488 1353
Size: 1104 168 699 120 714 1330 3423
Fragments arranged by size:
3423 1330 1104 714 699 168 120
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6145 6519 6665 387
Size: 1064 138 4556 374 146 1280
Fragments arranged by size:
4556 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2824 2824
Size: 7558
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2154 2493 2697 2945 3084 3201 3537
Size: 393 522 339 204 248 139 117 336
Cuts at: 3537 3822 3903 4219 4731 5621 6218 7005 7273
Size: 285 81 316 512 890 597 787 268
Cuts at: 7273 1239
Size: 1524
Fragments arranged by size:
1524 890 787 597 522 512 393 339
336 316 285 268 248 204 139 117
81
EcoNI CCTnn'n_nnAGG
Cuts at: 2924 2924
Size: 7558
EcoO109I rG'GnC_Cy
Cuts at: 2938 2938
Size: 7558
EcoRI G'AATT_C
Cuts at: 4867 5245 4867
Size: 378 7180
FspI TGC'GCA
Cuts at: 6064 6064
Size: 7558
HaeII r_GCGC'y
Cuts at: 1678 6705 1678
Size: 5027 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2045 2077 2165 2197 2564
Size: 32 135 32 473 32 88 32 367
Cuts at: 2564 2596 3119 3151 3566 3598 6199 6231 6273
Size: 32 523 32 415 32 2601 32 42
Cuts at: 6273 6305 1373
Size: 32 2626
Fragments arranged by size:
2626 2601 523 473 415 367 135 88
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3227 4488 4902 5148 669
Size: 988 281 1289 1261 414 246 3079
Fragments arranged by size:
3079 1289 1261 988 414 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7558
HpaI GTT'AAC
Cuts at: 5148 5148
Size: 7558
KpnI G_GTAC'C
Cuts at: 4883 4883
Size: 7558
MfeI C'AATT_G
Cuts at: 5157 5157
Size: 7558
MluI A'CGCG_T
Cuts at: 7434 7434
Size: 7558
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2642 3346 3451 6672 6856 1305
Size: 1337 704 105 3221 184 2007
Fragments arranged by size:
3221 2007 1337 704 184 105
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2898 4661 10
Size: 54 1425 343 1066 1763 2907
Fragments arranged by size:
2907 1763 1425 1066 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2552 3152 3821 4404 4613 5516 5875
Size: 648 1386 600 669 583 209 903 359
Cuts at: 5875 6034 518
Size: 159 2042
Fragments arranged by size:
2042 1386 903 669 648 600 583 359
209 159
MspA1I CmG'CkG
Cuts at: 1606 3881 4532 4824 4974 5651 6799 7044 1606
Size: 2275 651 292 150 677 1148 245 2120
Fragments arranged by size:
2275 2120 1148 677 651 292 245 150
NcoI C'CATG_G
Cuts at: 513 1065 2004 2057 3246 3285 513
Size: 552 939 53 1189 39 4786
Fragments arranged by size:
4786 1189 939 552 53 39
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7558
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7558
NotI GC'GGCC_GC
Cuts at: 5005 5005
Size: 7558
NspI r_CATG'y
Cuts at: 1132 1229 3973 4916 6461 1132
Size: 97 2744 943 1545 2229
Fragments arranged by size:
2744 2229 1545 943 97
PasI CC'CwG_GG
Cuts at: 1348 3597 1348
Size: 2249 5309
PciI A'CATG_T
Cuts at: 6457 6457
Size: 7558
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3986 1108
Size: 2878 4680
PfoI T'CCnGG_A
Cuts at: 2922 2922
Size: 7558
PmeI GTTT'AAAC
Cuts at: 4862 4862
Size: 7558
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5627 5659 7140 7172 5627
Size: 32 1481 32 6013
Fragments arranged by size:
6013 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2938 2938
Size: 7558
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7558
PsiI TTA'TAA
Cuts at: 5128 5128
Size: 7558
PstI C_TGCA'G
Cuts at: 830 3763 3880 4260 4393 4425 4910 830
Size: 2933 117 380 133 32 485 3478
Fragments arranged by size:
3478 2933 485 380 133 117 32
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7558
PvuII CAG'CTG
Cuts at: 3881 4532 3881
Size: 651 6907
SacI G_AGCT'C
Cuts at: 2826 2826
Size: 7558
SalI G'TCGA_C
Cuts at: 1936 4900 1936
Size: 2964 4594
SapI GCTCTTCn'nnn_
Cuts at: 3444 3444
Size: 7558
SbfI CC_TGCA'GG
Cuts at: 4910 4910
Size: 7558
ScaI AGT'ACT
Cuts at: 2207 3874 5806 7518 2207
Size: 1667 1932 1712 2247
Fragments arranged by size:
2247 1932 1712 1667
SfcI C'TryA_G
Cuts at: 826 1045 2490 2568 3123 3759 3876 4256 4318
Size: 219 1445 78 555 636 117 380 62
Cuts at: 4318 4389 4421 4906 6041 6722 6913 826
Size: 71 32 485 1135 681 191 1471
Fragments arranged by size:
1471 1445 1135 681 636 555 485 380
219 191 117 78 71 62 32
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7558
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7558
SmaI CCC'GGG
Cuts at: 1872 2656 4885 1872
Size: 784 2229 4545
Fragments arranged by size:
4545 2229 784
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5662 6563 6825 820
Size: 197 926 3719 901 262 1553
Fragments arranged by size:
3719 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5349 493
Size: 4856 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7558
SphI G_CATG'C
Cuts at: 1132 4916 1132
Size: 3784 3774
SspI AAT'ATT
Cuts at: 5 52 5482 5
Size: 47 5430 2081
Fragments arranged by size:
5430 2081 47
StuI AGG'CCT
Cuts at: 1517 4474 1517
Size: 2957 4601
StyI C'CwwG_G
Cuts at: 513 1065 2004 2057 3246 3285 4997 513
Size: 552 939 53 1189 39 1712 3074
Fragments arranged by size:
3074 1712 1189 939 552 53 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2992 5585 5602 5755 5940 6385 325
Size: 1255 1412 2593 17 153 185 445 1498
Fragments arranged by size:
2593 1498 1412 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2205 2909 3872
Size: 274 80 33 51 1443 228 704 963
Cuts at: 3872 4578 5804 7516 96
Size: 706 1226 1712 138
Fragments arranged by size:
1712 1443 1226 963 706 704 274 228
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2236 2253 2268 2285 2671 2695 2703 2727 7518
Size: 17 15 17 386 24 8 24 4791
Cuts at: 7518 7550 2236
Size: 32 2244
Fragments arranged by size:
4791 2244 386 32 24 24 17 17
15 8
Tth111I GACn'n_nGTC
Cuts at: 1316 4760 1316
Size: 3444 4114
XbaI T'CTAG_A
Cuts at: 4894 4894
Size: 7558
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3253 3253
Size: 7558
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7558
XmaI C'CCGG_G
Cuts at: 1870 2654 4883 1870
Size: 784 2229 4545
Fragments arranged by size:
4545 2229 784
XmnI GAAnn'nnTTC
Cuts at: 2388 4338 5687 2388
Size: 1950 1349 4259
Fragments arranged by size:
4259 1950 1349
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5366 276
Size: 53 83 186 936 3832 2468
Fragments arranged by size:
3832 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII
SanDI SexAI SfiI SfoI SrfI SwaI