Restriction map for
pFN21AB3671
(Circular) (Six-base) MAPSORT of: pFN21AB3671.seq Check: 7278 from: 1 to: 5833
pFN21AB3671 5833 bp
With 182 enzymes: SgfI *
October 20, 2009 10:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3643 278
Size: 53 83 186 936 2107 2468
Fragments arranged by size:
2468 2107 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3176 1937
Size: 1239 4594
Acc65I G'GTAC_C
Cuts at: 3154 3154
Size: 5833
AclI AA'CG_TT
Cuts at: 1563 2312 3960 4333 1563
Size: 749 1648 373 3063
Fragments arranged by size:
3063 1648 749 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2250 2372 3896 5280 5548 1632
Size: 618 122 1524 1384 268 1917
Fragments arranged by size:
1917 1524 1384 618 268 122
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5636
AflIII A'CryG_T
Cuts at: 1203 2085 4732 5709 1203
Size: 882 2647 977 1327
Fragments arranged by size:
2647 1327 977 882
AgeI A'CCGG_T
Cuts at: 4664 4664
Size: 5833
AhdI GACnn_n'nnGTC
Cuts at: 4562 4562
Size: 5833
AleI CACnn'nnGTG
Cuts at: 1166 2231 1166
Size: 1065 4768
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3043 1184
Size: 1859 3974
AlwNI CAG_nnn'CTG
Cuts at: 2176 2257 2653 3051 3576 5148 2176
Size: 81 396 398 525 1572 2861
Fragments arranged by size:
2861 1572 525 398 396 81
ApaLI G'TGCA_C
Cuts at: 2948 3890 5046 2948
Size: 942 1156 3735
Fragments arranged by size:
3735 1156 942
ApoI r'AATT_y
Cuts at: 1493 2816 3142 3368 3520 5629 1493
Size: 1323 326 226 152 2109 1697
Fragments arranged by size:
2109 1697 1323 326 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4387 160
Size: 872 3355 1606
Fragments arranged by size:
3355 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5833
AvaI C'yCGr_G
Cuts at: 1870 1943 3158 1870
Size: 73 1215 4545
Fragments arranged by size:
4545 1215 73
AvrII C'CTAG_G
Cuts at: 2134 2134
Size: 5833
BamHI G'GATC_C
Cuts at: 3163 3530 3163
Size: 367 5466
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2981 3154 4609 618
Size: 325 217 627 1194 173 1455 1842
Fragments arranged by size:
1842 1455 1194 627 325 217 173
BanII G_rGCy'C
Cuts at: 1391 2721 3152 1391
Size: 1330 431 4072
Fragments arranged by size:
4072 1330 431
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2202 2321 928
Size: 971 303 119 4440
Fragments arranged by size:
4440 971 303 119
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4024 4058 1903
Size: 34 2087 34 3678
Fragments arranged by size:
3678 2087 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3725 4935 3725
Size: 1210 4623
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5833
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4444 136
Size: 107 122 71 631 605 2772 1525
Fragments arranged by size:
2772 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5828 5828
Size: 5833
BlpI GC'TnA_GC
Cuts at: 2346 2681 2720 3250 2346
Size: 335 39 530 4929
Fragments arranged by size:
4929 530 335 39
Bme1580I G_kGCm'C
Cuts at: 1790 2952 3894 5050 1790
Size: 1162 942 1156 2573
Fragments arranged by size:
2573 1162 1156 942
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5833
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3564 4522 442
Size: 416 53 83 399 24 2147 958 1753
Fragments arranged by size:
2147 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5833
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2675 4493 1239
Size: 1436 1818 2579
Fragments arranged by size:
2579 1818 1436
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3958 4823 5121 3958
Size: 865 298 4670
Fragments arranged by size:
4670 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2416 2919 4496 882
Size: 617 917 503 1577 2219
Fragments arranged by size:
2219 1577 917 617 503
BsaAI yAC'GTr
Cuts at: 493 1204 2629 3624 493
Size: 711 1425 995 2702
Fragments arranged by size:
2702 1425 995 711
BsaBI GATnn'nnATC
Cuts at: 5827 5827
Size: 5833
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3640 4022 4657 275
Size: 53 83 186 936 2107 382 635 1451
Fragments arranged by size:
2107 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4266 4664 4938 5085 5496 4266
Size: 398 274 147 411 4603
Fragments arranged by size:
4603 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2597 2627 1178
Size: 30 165 30 1194 30 4384
Fragments arranged by size:
4384 1194 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2101 2588 1186
Size: 915 487 4431
Fragments arranged by size:
4431 915 487
BseYI C'CCAG_C
Cuts at: 5036 5532 5036
Size: 496 5337
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2609 1163
Size: 573 873 4387
Fragments arranged by size:
4387 873 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3283 4044 5072 1540
Size: 275 186 1282 761 1028 2301
Fragments arranged by size:
2301 1282 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2721 2952 3894 3979 5050 2721
Size: 231 942 85 1071 3504
Fragments arranged by size:
3504 1071 942 231 85
BsmI GAATG_Cn'
Cuts at: 3102 3344 3437 3102
Size: 242 93 5498
Fragments arranged by size:
5498 242 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2721 2952 3152 3894 3979 5050 1391
Size: 399 931 231 200 742 85 1071 2174
Fragments arranged by size:
2174 1071 931 742 399 231 200 85
BspEI T'CCGG_A
Cuts at: 5496 5496
Size: 5833
BspHI T'CATG_A
Cuts at: 3722 5452 3722
Size: 1730 4103
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2265 3108 3188 844
Size: 1421 843 80 3489
Fragments arranged by size:
3489 1421 843 80
BsrBI CCG'CTC
Cuts at: 1394 1823 3720 1394
Size: 429 1897 3507
Fragments arranged by size:
3507 1897 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2916 4328 4502 65
Size: 1183 1668 1412 174 1396
Fragments arranged by size:
1668 1412 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4477 4664 1700
Size: 110 2667 187 2869
Fragments arranged by size:
2869 2667 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5833
BssHII G'CGCG_C
Cuts at: 1925 2641 2643 1925
Size: 716 2 5115
Fragments arranged by size:
5115 716 2
BssSI C'ACGA_G
Cuts at: 3893 4905 3893
Size: 1012 4821
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5833
BstBI TT'CG_AA
Cuts at: 4637 4637
Size: 5833
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5833
BstYI r'GATC_y
Cuts at: 1972 3163 3530 3915 3932 5384 5470 5482 5828
Size: 1191 367 385 17 1452 86 12 346
Cuts at: 5828 1972
Size: 1977
Fragments arranged by size:
1977 1452 1191 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2124 513
Size: 552 939 120 4222
Fragments arranged by size:
4222 939 552 120
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3111 487
Size: 683 56 1885 3209
Fragments arranged by size:
3209 1885 683 56
BtsI GCAGTG_nn'
Cuts at: 3349 4143 4163 3349
Size: 794 20 5019
Fragments arranged by size:
5019 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5833
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5798
DraI TTT'AAA
Cuts at: 2052 2184 2560 2821 3137 2052
Size: 132 376 261 316 4748
Fragments arranged by size:
4748 376 316 261 132
DraIII CAC_nnn'GTG
Cuts at: 2086 2086
Size: 5833
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4840 809
Size: 507 3524 1802
Fragments arranged by size:
3524 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2121 2268 3280
Size: 54 1425 50 275 18 291 147 1012
Cuts at: 3280 4169 5798 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1012 889 291 275 147 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3280 1537
Size: 275 1468 4090
Fragments arranged by size:
4090 1468 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2376 2433 2780 3763 1353
Size: 1023 57 347 983 3423
Fragments arranged by size:
3423 1023 983 347 57
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4420 4794 4940 387
Size: 1064 138 2831 374 146 1280
Fragments arranged by size:
2831 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2719 2719
Size: 5833
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2250 2372 2675 3896 4493 5280 5548
Size: 393 618 122 303 1221 597 787 268
Cuts at: 5548 1239
Size: 1524
Fragments arranged by size:
1524 1221 787 618 597 393 303 268
122
EcoNI CCTnn'n_nnAGG
Cuts at: 2668 2668
Size: 5833
EcoRI G'AATT_C
Cuts at: 3142 3520 3142
Size: 378 5455
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2049 2760 2825 2917 2049
Size: 711 65 92 4965
Fragments arranged by size:
4965 711 92 65
FspI TGC'GCA
Cuts at: 4339 4339
Size: 5833
HaeII r_GCGC'y
Cuts at: 1678 4980 1678
Size: 3302 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2047 2079 2584 2616 2831
Size: 32 135 32 475 32 505 32 215
Cuts at: 2831 2863 4474 4506 4548 4580 1373
Size: 32 1611 32 42 32 2626
Fragments arranged by size:
2626 1611 505 475 215 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3177 3423 669
Size: 988 281 1239 246 3079
Fragments arranged by size:
3079 1239 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5833
HpaI GTT'AAC
Cuts at: 3423 3423
Size: 5833
KpnI G_GTAC'C
Cuts at: 3158 3158
Size: 5833
MfeI C'AATT_G
Cuts at: 2460 3432 2460
Size: 972 4861
MluI A'CGCG_T
Cuts at: 5709 5709
Size: 5833
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2908 4947 5131 1305
Size: 1603 2039 184 2007
Fragments arranged by size:
2039 2007 1603 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2123 10
Size: 54 1425 343 291 3720
Fragments arranged by size:
3720 1425 343 291 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2231 3791 4150 4309 518
Size: 648 1065 1560 359 159 2042
Fragments arranged by size:
2042 1560 1065 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2650 2918 2993 3249 3926 5074 5319 1606
Size: 1044 268 75 256 677 1148 245 2120
Fragments arranged by size:
2120 1148 1044 677 268 256 245 75
NcoI C'CATG_G
Cuts at: 513 1065 2004 2124 513
Size: 552 939 120 4222
Fragments arranged by size:
4222 939 552 120
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5833
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5833
NotI GC'GGCC_GC
Cuts at: 3280 3280
Size: 5833
NspI r_CATG'y
Cuts at: 1132 1229 3191 4736 1132
Size: 97 1962 1545 2229
Fragments arranged by size:
2229 1962 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5833
PciI A'CATG_T
Cuts at: 4732 4732
Size: 5833
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5833
PfoI T'CCnGG_A
Cuts at: 2387 2387
Size: 5833
PmeI GTTT'AAAC
Cuts at: 3137 3137
Size: 5833
PmlI CAC'GTG
Cuts at: 2629 2629
Size: 5833
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3902 3934 5415 5447 3902
Size: 32 1481 32 4288
Fragments arranged by size:
4288 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2931 1613
Size: 1318 4515
PsiI TTA'TAA
Cuts at: 3403 3403
Size: 5833
PstI C_TGCA'G
Cuts at: 830 2380 3185 830
Size: 1550 805 3478
Fragments arranged by size:
3478 1550 805
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5833
PvuII CAG'CTG
Cuts at: 2650 2650
Size: 5833
SacI G_AGCT'C
Cuts at: 2721 2721
Size: 5833
SalI G'TCGA_C
Cuts at: 1936 3175 1936
Size: 1239 4594
SbfI CC_TGCA'GG
Cuts at: 3185 3185
Size: 5833
ScaI AGT'ACT
Cuts at: 2791 4081 5793 2791
Size: 1290 1712 2831
Fragments arranged by size:
2831 1712 1290
SfcI C'TryA_G
Cuts at: 826 1045 2376 3181 4316 4997 5188 826
Size: 219 1331 805 1135 681 191 1471
Fragments arranged by size:
1471 1331 1135 805 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5833
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5833
SmaI CCC'GGG
Cuts at: 1872 3160 1872
Size: 1288 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3937 4838 5100 820
Size: 197 926 1994 901 262 1553
Fragments arranged by size:
1994 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3624 493
Size: 3131 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5833
SphI G_CATG'C
Cuts at: 1132 3191 1132
Size: 2059 3774
SspI AAT'ATT
Cuts at: 5 52 3757 5
Size: 47 3705 2081
Fragments arranged by size:
3705 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5833
StyI C'CwwG_G
Cuts at: 513 1065 2004 2124 2134 3272 513
Size: 552 939 120 10 1138 3074
Fragments arranged by size:
3074 1138 939 552 120 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2438 3860 3877 4030 4215 4660 325
Size: 1255 858 1422 17 153 185 445 1498
Fragments arranged by size:
1498 1422 1255 858 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2789 4079 5791
Size: 274 80 33 51 1443 812 1290 1712
Cuts at: 5791 96
Size: 138
Fragments arranged by size:
1712 1443 1290 812 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5793 5825 5793
Size: 32 5801
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5833
XbaI T'CTAG_A
Cuts at: 3169 3169
Size: 5833
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5833
XmaI C'CCGG_G
Cuts at: 1870 3158 1870
Size: 1288 4545
XmnI GAAnn'nnTTC
Cuts at: 2199 3962 2199
Size: 1763 4070
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3641 276
Size: 53 83 186 936 2107 2468
Fragments arranged by size:
2468 2107 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PpuMI PspOMI PspXI PsrI RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI