Restriction map for
pFN21AB3654
(Circular) (Six-base) MAPSORT of: pFN21AB3654.seq Check: 7820 from: 1 to: 7792
pFN21AB3654 7792 bp
With 182 enzymes: SgfI *
April 15, 2010 09:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5602 278
Size: 53 83 186 936 4066 2468
Fragments arranged by size:
4066 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5135 1937
Size: 3198 4594
Acc65I G'GTAC_C
Cuts at: 5113 5113
Size: 7792
AclI AA'CG_TT
Cuts at: 1563 5919 6292 1563
Size: 4356 373 3063
Fragments arranged by size:
4356 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2589 2670 3117 3587 4007 4427 4468 5855
Size: 957 81 447 470 420 420 41 1387
Cuts at: 5855 7239 7507 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1387 1384 957 470 447 420 420
268 81 41
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7595
AflIII A'CryG_T
Cuts at: 1203 6691 7668 1203
Size: 5488 977 1327
Fragments arranged by size:
5488 1327 977
AgeI A'CCGG_T
Cuts at: 6623 6623
Size: 7792
AhdI GACnn_n'nnGTC
Cuts at: 6521 6521
Size: 7792
AleI CACnn'nnGTG
Cuts at: 1166 3183 4359 1166
Size: 2017 1176 4599
Fragments arranged by size:
4599 2017 1176
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7792
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2443 2475 2443
Size: 32 7760
AlwNI CAG_nnn'CTG
Cuts at: 2248 2275 5535 7107 2248
Size: 27 3260 1572 2933
Fragments arranged by size:
3260 2933 1572 27
ApaI G_GGCC'C
Cuts at: 2711 2711
Size: 7792
ApaLI G'TGCA_C
Cuts at: 5849 7005 5849
Size: 1156 6636
ApoI r'AATT_y
Cuts at: 1493 2496 4434 4602 4834 4854 4985 5039 5101
Size: 1003 1938 168 232 20 131 54 62
Cuts at: 5101 5327 5479 7588 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1938 1697 1003 232 226 168 152
131 62 54 20
AseI AT'TA_AT
Cuts at: 160 1032 2612 6346 160
Size: 872 1580 3734 1606
Fragments arranged by size:
3734 1606 1580 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7792
AvaI C'yCGr_G
Cuts at: 1870 1943 5117 1870
Size: 73 3174 4545
Fragments arranged by size:
4545 3174 73
BamHI G'GATC_C
Cuts at: 2542 3342 4518 5122 5489 2542
Size: 800 1176 604 367 4845
Fragments arranged by size:
4845 1176 800 604 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3426 5113 6568 618
Size: 325 217 627 1639 1687 1455 1842
Fragments arranged by size:
1842 1687 1639 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2711 5111 1391
Size: 1320 2400 4072
Fragments arranged by size:
4072 2400 1320
BbeI G_GCGC'C
Cuts at: 3430 3430
Size: 7792
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3466 4054 4474 928
Size: 971 1567 588 420 4246
Fragments arranged by size:
4246 1567 971 588 420
BbvCI CC'TCA_GC
Cuts at: 2547 2547
Size: 7792
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5983 6017 1903
Size: 34 4046 34 3678
Fragments arranged by size:
4046 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5684 6894 5684
Size: 1210 6582
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7792
BfrBI ATG'CAT
Cuts at: 3250 3250
Size: 7792
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6403 136
Size: 107 122 71 631 605 4731 1525
Fragments arranged by size:
4731 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7787 7787
Size: 7792
BlpI GC'TnA_GC
Cuts at: 5209 5209
Size: 7792
Bme1580I G_kGCm'C
Cuts at: 1790 2446 2711 5853 7009 1790
Size: 656 265 3142 1156 2573
Fragments arranged by size:
3142 2573 1156 656 265
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7792
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2740 3360 4788
Size: 416 53 83 399 24 1323 620 1428
Cuts at: 4788 5523 6481 442
Size: 735 958 1753
Fragments arranged by size:
1753 1428 1323 958 735 620 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7792
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4049 5059 4049
Size: 1010 6782
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2270 2285 2411 2522 2765 4044 4716 6452
Size: 1031 15 126 111 243 1279 672 1736
Cuts at: 6452 1239
Size: 2579
Fragments arranged by size:
2579 1736 1279 1031 672 243 126 111
15
Bpu10I CC'TnA_GC
Cuts at: 2183 2192 2547 2183
Size: 9 355 7428
Fragments arranged by size:
7428 355 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5917 6782 7080 5917
Size: 865 298 6629
Fragments arranged by size:
6629 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2114 2212 2774 3436 6455 882
Size: 617 615 98 562 662 3019 2219
Fragments arranged by size:
3019 2219 662 617 615 562 98
BsaAI yAC'GTr
Cuts at: 493 1204 4572 5583 493
Size: 711 3368 1011 2702
Fragments arranged by size:
3368 2702 1011 711
BsaBI GATnn'nnATC
Cuts at: 7786 7786
Size: 7792
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3427 5599 5981 6616
Size: 53 83 186 936 1894 2172 382 635
Cuts at: 6616 275
Size: 1451
Fragments arranged by size:
2172 1894 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4274 6225 6623 6897 7044 7455 4274
Size: 1951 398 274 147 411 4611
Fragments arranged by size:
4611 1951 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2446 2476 2610 2640 1178
Size: 30 165 30 1043 30 134 30 6330
Fragments arranged by size:
6330 1043 165 134 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2098 2351 2372 2678 2813 3737 1186
Size: 912 253 21 306 135 924 5241
Fragments arranged by size:
5241 924 912 306 253 135 21
BseYI C'CCAG_C
Cuts at: 2637 6995 7491 2637
Size: 4358 496 2938
Fragments arranged by size:
4358 2938 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3569 3708 1163
Size: 573 1833 139 5247
Fragments arranged by size:
5247 1833 573 139
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5242 6003 7031 1540
Size: 275 186 3241 761 1028 2301
Fragments arranged by size:
3241 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5853 5938 7009 5853
Size: 85 1071 6636
Fragments arranged by size:
6636 1071 85
BsmI GAATG_Cn'
Cuts at: 2528 3490 4298 4776 5303 5396 2528
Size: 962 808 478 527 93 4924
Fragments arranged by size:
4924 962 808 527 478 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2446 2711 5111 5853 5938 7009 1391
Size: 399 656 265 2400 742 85 1071 2174
Fragments arranged by size:
2400 2174 1071 742 656 399 265 85
BspEI T'CCGG_A
Cuts at: 7455 7455
Size: 7792
BspHI T'CATG_A
Cuts at: 5681 7411 5681
Size: 1730 6062
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5147 844
Size: 4303 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4494 5679 1394
Size: 429 2671 1185 3507
Fragments arranged by size:
3507 2671 1185 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4471 6287 6461 65
Size: 1183 3223 1816 174 1396
Fragments arranged by size:
3223 1816 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2187 2208 6436 6623 1700
Size: 110 377 21 4228 187 2869
Fragments arranged by size:
4228 2869 377 187 110 21
BsrGI T'GTAC_A
Cuts at: 96 4255 4759 4927 96
Size: 4159 504 168 2961
Fragments arranged by size:
4159 2961 504 168
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7792
BssSI C'ACGA_G
Cuts at: 4441 5852 6864 4441
Size: 1411 1012 5369
Fragments arranged by size:
5369 1411 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7792
BstBI TT'CG_AA
Cuts at: 3733 6596 3733
Size: 2863 4929
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7792
BstXI CCAn_nnnn'nTGG
Cuts at: 2324 2324
Size: 7792
BstYI r'GATC_y
Cuts at: 1972 2244 2542 3342 4518 5122 5489 5874 5891
Size: 272 298 800 1176 604 367 385 17
Cuts at: 5891 7343 7429 7441 7787 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1176 800 604 385 367 346
298 272 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 7240
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7053
Fragments arranged by size:
7053 683 56
BtsI GCAGTG_nn'
Cuts at: 4303 5308 6102 6122 4303
Size: 1005 794 20 5973
Fragments arranged by size:
5973 1005 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7792
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4453 4488 529
Size: 35 3889 35 3833
Fragments arranged by size:
3889 3833 35 35
DraI TTT'AAA
Cuts at: 5096 5096
Size: 7792
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6799 809
Size: 507 5483 1802
Fragments arranged by size:
5483 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4843 5239 6128
Size: 54 1425 50 275 18 3013 396 889
Cuts at: 6128 7757 8
Size: 1629 43
Fragments arranged by size:
3013 1629 1425 889 396 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5239 1537
Size: 275 3427 4090
Fragments arranged by size:
4090 3427 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2586 2895 3566 3986 4303 5722 1353
Size: 1233 309 671 420 317 1419 3423
Fragments arranged by size:
3423 1419 1233 671 420 317 309
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6379 6753 6899 387
Size: 1064 138 4790 374 146 1280
Fragments arranged by size:
4790 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2270 2285 2411 2522 2589 2670 2765
Size: 393 638 15 126 111 67 81 95
Cuts at: 2765 3117 3587 4007 4044 4427 4468 4716 5855
Size: 352 470 420 37 383 41 248 1139
Cuts at: 5855 6452 7239 7507 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1139 787 638 597 470 420 393
383 352 268 248 126 111 95 81
67 41 37 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2054 2354 2408 2585 2054
Size: 300 54 177 7261
Fragments arranged by size:
7261 300 177 54
EcoO109I rG'GnC_Cy
Cuts at: 2047 2721 2884 2047
Size: 674 163 6955
Fragments arranged by size:
6955 674 163
EcoRI G'AATT_C
Cuts at: 4434 4602 4854 4985 5039 5101 5479 4434
Size: 168 252 131 54 62 378 6747
Fragments arranged by size:
6747 378 252 168 131 62 54
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2066 2996 3799 3844 2066
Size: 930 803 45 6014
Fragments arranged by size:
6014 930 803 45
FspI TGC'GCA
Cuts at: 6298 6298
Size: 7792
HaeII r_GCGC'y
Cuts at: 1678 3430 6939 1678
Size: 1752 3509 2531
Fragments arranged by size:
3509 2531 1752
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2654 2686 2792 2824 3631
Size: 32 135 32 1082 32 106 32 807
Cuts at: 3631 3643 3663 3675 4579 4611 6433 6465 6507
Size: 12 20 12 904 32 1822 32 42
Cuts at: 6507 6539 1373
Size: 32 2626
Fragments arranged by size:
2626 1822 1082 904 807 135 106 42
32 32 32 32 32 32 32 20
12 12
HincII GTy'rAC
Cuts at: 669 1657 1938 5136 5382 669
Size: 988 281 3198 246 3079
Fragments arranged by size:
3198 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2571 3555 748
Size: 1823 984 4985
Fragments arranged by size:
4985 1823 984
HpaI GTT'AAC
Cuts at: 5382 5382
Size: 7792
KasI G'GCGC_C
Cuts at: 3426 3426
Size: 7792
KpnI G_GTAC'C
Cuts at: 5117 5117
Size: 7792
MfeI C'AATT_G
Cuts at: 5391 5391
Size: 7792
MluI A'CGCG_T
Cuts at: 7668 7668
Size: 7792
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5025 6906 7090 1305
Size: 3720 1881 184 2007
Fragments arranged by size:
3720 2007 1881 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4845 10
Size: 54 1425 343 3013 2957
Fragments arranged by size:
3013 2957 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3183 4123 4359 4527 4947 5750 6109
Size: 648 2017 940 236 168 420 803 359
Cuts at: 6109 6268 518
Size: 159 2042
Fragments arranged by size:
2042 2017 940 803 648 420 359 236
168 159
MspA1I CmG'CkG
Cuts at: 1606 2492 5208 5885 7033 7278 1606
Size: 886 2716 677 1148 245 2120
Fragments arranged by size:
2716 2120 1148 886 677 245
NaeI GCC'GGC
Cuts at: 2189 2189
Size: 7792
NarI GG'CG_CC
Cuts at: 3427 3427
Size: 7792
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7240
NdeI CA'TA_TG
Cuts at: 387 4120 387
Size: 3733 4059
NgoMIV G'CCGG_C
Cuts at: 2187 2187
Size: 7792
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7792
NotI GC'GGCC_GC
Cuts at: 5239 5239
Size: 7792
NsiI A_TGCA'T
Cuts at: 3252 3252
Size: 7792
NspI r_CATG'y
Cuts at: 1132 1229 2703 5150 6695 1132
Size: 97 1474 2447 1545 2229
Fragments arranged by size:
2447 2229 1545 1474 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7792
PciI A'CATG_T
Cuts at: 6691 6691
Size: 7792
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2434 2650 1108
Size: 1326 216 6250
Fragments arranged by size:
6250 1326 216
PfoI T'CCnGG_A
Cuts at: 2247 2538 2247
Size: 291 7501
PmeI GTTT'AAAC
Cuts at: 5096 5096
Size: 7792
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5861 5893 7374 7406 5861
Size: 32 1481 32 6247
Fragments arranged by size:
6247 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2884 2884
Size: 7792
PshAI GACnn'nnGTC
Cuts at: 1613 4737 1613
Size: 3124 4668
PsiI TTA'TAA
Cuts at: 3114 3161 3282 3366 3450 3534 4458 4710 5362
Size: 47 121 84 84 84 924 252 652
Cuts at: 5362 3114
Size: 5544
Fragments arranged by size:
5544 924 652 252 121 84 84 84
47
PspOMI G'GGCC_C
Cuts at: 2707 2707
Size: 7792
PstI C_TGCA'G
Cuts at: 830 2367 5144 830
Size: 1537 2777 3478
Fragments arranged by size:
3478 2777 1537
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7792
PvuII CAG'CTG
Cuts at: 2492 2492
Size: 7792
SalI G'TCGA_C
Cuts at: 1936 5134 1936
Size: 3198 4594
SapI GCTCTTCn'nnn_
Cuts at: 3566 3986 4303 3566
Size: 420 317 7055
Fragments arranged by size:
7055 420 317
SbfI CC_TGCA'GG
Cuts at: 5144 5144
Size: 7792
ScaI AGT'ACT
Cuts at: 6040 7752 6040
Size: 1712 6080
SfcI C'TryA_G
Cuts at: 826 1045 2363 3394 5140 6275 6956 7147 826
Size: 219 1318 1031 1746 1135 681 191 1471
Fragments arranged by size:
1746 1471 1318 1135 1031 681 219 191
SfoI GGC'GCC
Cuts at: 3428 3428
Size: 7792
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7792
SgrAI Cr'CCGG_yG
Cuts at: 1700 2208 1700
Size: 508 7284
SmaI CCC'GGG
Cuts at: 1872 5119 1872
Size: 3247 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5896 6797 7059 820
Size: 197 926 3953 901 262 1553
Fragments arranged by size:
3953 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4572 5583 493
Size: 4079 1011 2702
Fragments arranged by size:
4079 2702 1011
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7792
SphI G_CATG'C
Cuts at: 1132 5150 1132
Size: 4018 3774
SspI AAT'ATT
Cuts at: 5 52 5716 5
Size: 47 5664 2081
Fragments arranged by size:
5664 2081 47
StuI AGG'CCT
Cuts at: 1517 3408 1517
Size: 1891 5901
StyI C'CwwG_G
Cuts at: 513 1065 2095 2235 5231 513
Size: 552 1030 140 2996 3074
Fragments arranged by size:
3074 2996 1030 552 140
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2804 4869 5819 5836 5989 6174 6619
Size: 1255 1224 2065 950 17 153 185 445
Cuts at: 6619 325
Size: 1498
Fragments arranged by size:
2065 1498 1255 1224 950 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2467 2727 4255
Size: 274 80 33 51 1443 490 260 1528
Cuts at: 4255 4759 4927 6038 7750 96
Size: 504 168 1111 1712 138
Fragments arranged by size:
1712 1528 1443 1111 504 490 274 260
168 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2610 2642 7752 7784 2610
Size: 32 5110 32 2618
Fragments arranged by size:
5110 2618 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7792
XbaI T'CTAG_A
Cuts at: 5128 5128
Size: 7792
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2162 2162
Size: 7792
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7792
XmaI C'CCGG_G
Cuts at: 1870 5117 1870
Size: 3247 4545
XmnI GAAnn'nnTTC
Cuts at: 3473 3557 3809 4229 4481 5921 3473
Size: 84 252 420 252 1440 5344
Fragments arranged by size:
5344 1440 420 252 252 84
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5600 276
Size: 53 83 186 936 4066 2468
Fragments arranged by size:
4066 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BsiWI BsmBI BstZ17I
Bsu36I DraIII EcoICRI EcoRV FseI FspAI NruI PacI
PmlI PspXI PsrI RsrII SacI SacII SanDI SexAI
SfiI SrfI SwaI