Restriction map for
pFN21AB3652
(Circular) (Six-base) MAPSORT of: pFN21AB3652.seq Check: 6025 from: 1 to: 7009
pFN21AB3652 7009 bp
With 182 enzymes: SgfI *
October 20, 2009 10:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4819 278
Size: 53 83 186 936 3283 2468
Fragments arranged by size:
3283 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2319 4352 1937
Size: 382 2033 4594
Fragments arranged by size:
4594 2033 382
Acc65I G'GTAC_C
Cuts at: 4330 4330
Size: 7009
AclI AA'CG_TT
Cuts at: 1563 5136 5509 1563
Size: 3573 373 3063
Fragments arranged by size:
3573 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3876 4029 4185 4301 5072 6456 6724 1632
Size: 2244 153 156 116 771 1384 268 1917
Fragments arranged by size:
2244 1917 1384 771 268 156 153 116
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6812
AflIII A'CryG_T
Cuts at: 1203 5908 6885 1203
Size: 4705 977 1327
Fragments arranged by size:
4705 1327 977
AgeI A'CCGG_T
Cuts at: 5840 5840
Size: 7009
AhdI GACnn_n'nnGTC
Cuts at: 5738 5738
Size: 7009
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7009
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2214 1184
Size: 1030 5979
AlwNI CAG_nnn'CTG
Cuts at: 2741 4752 6324 2741
Size: 2011 1572 3426
Fragments arranged by size:
3426 2011 1572
ApaI G_GGCC'C
Cuts at: 3779 3779
Size: 7009
ApaLI G'TGCA_C
Cuts at: 2717 5066 6222 2717
Size: 2349 1156 3504
Fragments arranged by size:
3504 2349 1156
ApoI r'AATT_y
Cuts at: 1493 3131 3625 4318 4544 4696 6805 1493
Size: 1638 494 693 226 152 2109 1697
Fragments arranged by size:
2109 1697 1638 693 494 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5563 160
Size: 872 4531 1606
Fragments arranged by size:
4531 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7009
AvaI C'yCGr_G
Cuts at: 1870 1943 4133 4334 1870
Size: 73 2190 201 4545
Fragments arranged by size:
4545 2190 201 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2292 2325 2292
Size: 33 6976
BamHI G'GATC_C
Cuts at: 4339 4706 4339
Size: 367 6642
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2128 4330 5785 618
Size: 325 217 627 341 2202 1455 1842
Fragments arranged by size:
2202 1842 1455 627 341 325 217
BanII G_rGCy'C
Cuts at: 1391 2916 3779 3854 4328 1391
Size: 1525 863 75 474 4072
Fragments arranged by size:
4072 1525 863 474 75
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3341 3416 3963 928
Size: 971 1442 75 547 3974
Fragments arranged by size:
3974 1442 971 547 75
BbvCI CC'TCA_GC
Cuts at: 4180 4180
Size: 7009
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5200 5234 1903
Size: 34 3263 34 3678
Fragments arranged by size:
3678 3263 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4901 6111 4901
Size: 1210 5799
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7009
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3725 4160 5620
Size: 107 122 71 631 605 2053 435 1460
Cuts at: 5620 136
Size: 1525
Fragments arranged by size:
2053 1525 1460 631 605 435 122 107
71
BglII A'GATC_T
Cuts at: 2473 7004 2473
Size: 4531 2478
BlpI GC'TnA_GC
Cuts at: 4426 4426
Size: 7009
Bme1580I G_kGCm'C
Cuts at: 1790 2721 3290 3779 5070 6226 1790
Size: 931 569 489 1291 1156 2573
Fragments arranged by size:
2573 1291 1156 931 569 489
BmgBI CAC'GTC
Cuts at: 1318 2704 3757 1318
Size: 1386 1053 4570
Fragments arranged by size:
4570 1386 1053
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4740 5698 442
Size: 416 53 83 399 24 3323 958 1753
Fragments arranged by size:
3323 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7009
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3833 3885 3971 4002 4212 5669 1239
Size: 2594 52 86 31 210 1457 2579
Fragments arranged by size:
2594 2579 1457 210 86 52 31
Bpu10I CC'TnA_GC
Cuts at: 4180 4180
Size: 7009
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2131 5134 5999 6297 2131
Size: 3003 865 298 2843
Fragments arranged by size:
3003 2843 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2493 2752 3081 4230 5672 882
Size: 617 994 259 329 1149 1442 2219
Fragments arranged by size:
2219 1442 1149 994 617 329 259
BsaAI yAC'GTr
Cuts at: 493 1204 3445 4800 493
Size: 711 2241 1355 2702
Fragments arranged by size:
2702 2241 1355 711
BsaBI GATnn'nnATC
Cuts at: 7003 7003
Size: 7009
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4816 5198 5833 275
Size: 53 83 186 936 3283 382 635 1451
Fragments arranged by size:
3283 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2901 3079 3473 5442 5840 6114 6261 6672 2901
Size: 178 394 1969 398 274 147 411 3238
Fragments arranged by size:
3238 1969 411 398 394 274 178 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2607 2637 2919 2949 3696
Size: 30 165 30 1204 30 282 30 747
Cuts at: 3696 3726 1178
Size: 30 4461
Fragments arranged by size:
4461 1204 747 282 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2954 3830 4094 4205 1186
Size: 1768 876 264 111 3990
Fragments arranged by size:
3990 1768 876 264 111
BseYI C'CCAG_C
Cuts at: 2323 2731 3932 4150 6212 6708 2323
Size: 408 1201 218 2062 496 2624
Fragments arranged by size:
2624 2062 1201 496 408 218
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2676 3027 1163
Size: 573 940 351 5145
Fragments arranged by size:
5145 940 573 351
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2083 4459 5220 6248 1540
Size: 275 186 82 2376 761 1028 2301
Fragments arranged by size:
2376 2301 1028 761 275 186 82
BsiHKAI G_wGCw'C
Cuts at: 2721 3770 5070 5155 6226 2721
Size: 1049 1300 85 1071 3504
Fragments arranged by size:
3504 1300 1071 1049 85
BsmI GAATG_Cn'
Cuts at: 2829 4520 4613 2829
Size: 1691 93 5225
Fragments arranged by size:
5225 1691 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2721 2916 3290 3770 3779 3854 4328
Size: 399 931 195 374 480 9 75 474
Cuts at: 4328 5070 5155 6226 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 931 742 480 474 399 374
195 85 75 9
BspEI T'CCGG_A
Cuts at: 2901 6672 2901
Size: 3771 3238
BspHI T'CATG_A
Cuts at: 2815 4898 6628 2815
Size: 2083 1730 3196
Fragments arranged by size:
3196 2083 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4364 844
Size: 3520 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4896 1394
Size: 429 3073 3507
Fragments arranged by size:
3507 3073 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2760 5504 5678 65
Size: 1183 1512 2744 174 1396
Fragments arranged by size:
2744 1512 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2660 5653 5840 1700
Size: 110 850 2993 187 2869
Fragments arranged by size:
2993 2869 850 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7009
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7009
BssSI C'ACGA_G
Cuts at: 2019 2915 5069 6081 2019
Size: 896 2154 1012 2947
Fragments arranged by size:
2947 2154 1012 896
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2687 2741 794
Size: 1893 54 5062
Fragments arranged by size:
5062 1893 54
BstBI TT'CG_AA
Cuts at: 2992 5813 2992
Size: 2821 4188
BstEII G'GTnAC_C
Cuts at: 1184 2032 1184
Size: 848 6161
BstXI CCAn_nnnn'nTGG
Cuts at: 3949 3949
Size: 7009
BstYI r'GATC_y
Cuts at: 1972 2399 2473 2982 3612 4339 4706 5091 5108
Size: 427 74 509 630 727 367 385 17
Cuts at: 5108 6560 6646 6658 7004 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 727 630 509 427 385 367
346 86 74 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2824 2908 3771 4203 513
Size: 552 1759 84 863 432 3319
Fragments arranged by size:
3319 1759 863 552 432 84
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3243 4233 487
Size: 683 56 2017 990 3263
Fragments arranged by size:
3263 2017 990 683 56
BtsI GCAGTG_nn'
Cuts at: 4525 5319 5339 4525
Size: 794 20 6195
Fragments arranged by size:
6195 794 20
ClaI AT'CG_AT
Cuts at: 1554 3190 1554
Size: 1636 5373
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6974
DraI TTT'AAA
Cuts at: 4313 4313
Size: 7009
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3026 6016 809
Size: 507 1710 2990 1802
Fragments arranged by size:
2990 1802 1710 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3000 3355 4456
Size: 54 1425 50 275 18 1170 355 1101
Cuts at: 4456 5345 6974 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1170 1101 889 355 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4456 1537
Size: 275 2644 4090
Fragments arranged by size:
4090 2644 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2165 2838 3852 4161 4280 4292 4939 1353
Size: 812 673 1014 309 119 12 647 3423
Fragments arranged by size:
3423 1014 812 673 647 309 119 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5596 5970 6116 387
Size: 1064 138 4007 374 146 1280
Fragments arranged by size:
4007 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3833 3876 3885 3971 4002 4029 4185
Size: 393 2201 43 9 86 31 27 156
Cuts at: 4185 4212 4301 5072 5669 6456 6724 1239
Size: 27 89 771 597 787 268 1524
Fragments arranged by size:
2201 1524 787 771 597 393 268 156
89 86 43 31 27 27 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2141 3698 2141
Size: 1557 5452
EcoO109I rG'GnC_Cy
Cuts at: 2876 3776 4114 2876
Size: 900 338 5771
Fragments arranged by size:
5771 900 338
EcoRI G'AATT_C
Cuts at: 4318 4696 4318
Size: 378 6631
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2217 2217
Size: 7009
FspI TGC'GCA
Cuts at: 5515 5515
Size: 7009
HaeII r_GCGC'y
Cuts at: 1678 2792 6156 1678
Size: 1114 3364 2531
Fragments arranged by size:
3364 2531 1114
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5650 5682 5724 5756 1373
Size: 32 135 32 4078 32 42 32 2626
Fragments arranged by size:
4078 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4353 4599 669
Size: 988 281 2415 246 3079
Fragments arranged by size:
3079 2415 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3369 748
Size: 2621 4388
HpaI GTT'AAC
Cuts at: 4599 4599
Size: 7009
KpnI G_GTAC'C
Cuts at: 4334 4334
Size: 7009
MfeI C'AATT_G
Cuts at: 4608 4608
Size: 7009
MluI A'CGCG_T
Cuts at: 6885 6885
Size: 7009
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2905 6123 6307 1305
Size: 1600 3218 184 2007
Fragments arranged by size:
3218 2007 1600 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3002 10
Size: 54 1425 343 1170 4017
Fragments arranged by size:
4017 1425 1170 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3149 4967 5326 5485 518
Size: 648 1983 1818 359 159 2042
Fragments arranged by size:
2042 1983 1818 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3328 4184 4205 4425 5102 6250 6495 1606
Size: 1722 856 21 220 677 1148 245 2120
Fragments arranged by size:
2120 1722 1148 856 677 245 220 21
NaeI GCC'GGC
Cuts at: 2662 2662
Size: 7009
NcoI C'CATG_G
Cuts at: 513 1065 2824 2908 513
Size: 552 1759 84 4614
Fragments arranged by size:
4614 1759 552 84
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7009
NgoMIV G'CCGG_C
Cuts at: 2660 2660
Size: 7009
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7009
NotI GC'GGCC_GC
Cuts at: 4456 4456
Size: 7009
NspI r_CATG'y
Cuts at: 1132 1229 4367 5912 1132
Size: 97 3138 1545 2229
Fragments arranged by size:
3138 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3071 1348
Size: 1723 5286
PciI A'CATG_T
Cuts at: 5908 5908
Size: 7009
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7009
PfoI T'CCnGG_A
Cuts at: 3594 3594
Size: 7009
PmeI GTTT'AAAC
Cuts at: 4313 4313
Size: 7009
PmlI CAC'GTG
Cuts at: 3445 3445
Size: 7009
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2529 2561 5078 5110 6591 6623 2529
Size: 32 2517 32 1481 32 2915
Fragments arranged by size:
2915 2517 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7009
PsiI TTA'TAA
Cuts at: 4579 4579
Size: 7009
PspOMI G'GGCC_C
Cuts at: 3775 3775
Size: 7009
PstI C_TGCA'G
Cuts at: 830 3462 4361 830
Size: 2632 899 3478
Fragments arranged by size:
3478 2632 899
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7009
PvuII CAG'CTG
Cuts at: 4184 4184
Size: 7009
SacII CC_GC'GG
Cuts at: 4206 4206
Size: 7009
SalI G'TCGA_C
Cuts at: 1936 4351 1936
Size: 2415 4594
SapI GCTCTTCn'nnn_
Cuts at: 4280 4280
Size: 7009
SbfI CC_TGCA'GG
Cuts at: 4361 4361
Size: 7009
ScaI AGT'ACT
Cuts at: 2108 5257 6969 2108
Size: 3149 1712 2148
Fragments arranged by size:
3149 2148 1712
SfcI C'TryA_G
Cuts at: 826 1045 3169 3458 4357 5492 6173 6364 826
Size: 219 2124 289 899 1135 681 191 1471
Fragments arranged by size:
2124 1471 1135 899 681 289 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3725 3725
Size: 7009
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7009
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7009
SmaI CCC'GGG
Cuts at: 1872 4336 1872
Size: 2464 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2110 5113 6014 6276 820
Size: 197 926 167 3003 901 262 1553
Fragments arranged by size:
3003 1553 926 901 262 197 167
SnaBI TAC'GTA
Cuts at: 493 4800 493
Size: 4307 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7009
SphI G_CATG'C
Cuts at: 1132 4367 1132
Size: 3235 3774
SspI AAT'ATT
Cuts at: 5 52 4933 5
Size: 47 4881 2081
Fragments arranged by size:
4881 2081 47
StuI AGG'CCT
Cuts at: 1517 2265 2945 1517
Size: 748 680 5581
Fragments arranged by size:
5581 748 680
StyI C'CwwG_G
Cuts at: 513 1065 2797 2824 2908 3723 4448 513
Size: 552 1732 27 84 815 725 3074
Fragments arranged by size:
3074 1732 815 725 552 84 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2097 2472 5036 5053 5206 5391 5836
Size: 1255 517 375 2564 17 153 185 445
Cuts at: 5836 325
Size: 1498
Fragments arranged by size:
2564 1498 1255 517 445 375 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2106 3048 5255
Size: 274 80 33 51 1443 129 942 2207
Cuts at: 5255 6967 96
Size: 1712 138
Fragments arranged by size:
2207 1712 1443 942 274 138 129 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2693 2725 3696 3728 4044 4076 6969 7001 2693
Size: 32 971 32 316 32 2893 32 2701
Fragments arranged by size:
2893 2701 971 316 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7009
XbaI T'CTAG_A
Cuts at: 4345 4345
Size: 7009
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2266 3668 2266
Size: 1402 5607
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7009
XmaI C'CCGG_G
Cuts at: 1870 4334 1870
Size: 2464 4545
XmnI GAAnn'nnTTC
Cuts at: 4259 5138 4259
Size: 879 6130
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4817 276
Size: 53 83 186 936 3283 2468
Fragments arranged by size:
3283 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SacII SalI SapI SbfI ScaI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI
FspAI KasI NarI NruI NsiI PacI PpuMI PspXI
PsrI RsrII SacI SanDI SexAI SfoI SrfI SwaI