Restriction map for
pFN21AB3640
(Circular) (Six-base) MAPSORT of: pFN21AB3640.seq Check: 4679 from: 1 to: 5962
pFN21AB3640 5962 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3772 278
Size: 53 83 186 936 2236 2468
Fragments arranged by size:
2468 2236 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3305 1937
Size: 1368 4594
Acc65I G'GTAC_C
Cuts at: 3283 3283
Size: 5962
AclI AA'CG_TT
Cuts at: 1563 4089 4462 1563
Size: 2526 373 3063
Fragments arranged by size:
3063 2526 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4025 5409 5677 1632
Size: 2393 1384 268 1917
Fragments arranged by size:
2393 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5765
AflIII A'CryG_T
Cuts at: 1203 4861 5838 1203
Size: 3658 977 1327
Fragments arranged by size:
3658 1327 977
AgeI A'CCGG_T
Cuts at: 4793 4793
Size: 5962
AhdI GACnn_n'nnGTC
Cuts at: 4691 4691
Size: 5962
AleI CACnn'nnGTG
Cuts at: 1166 2966 1166
Size: 1800 4162
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5962
AlwNI CAG_nnn'CTG
Cuts at: 3705 5277 3705
Size: 1572 4390
ApaLI G'TGCA_C
Cuts at: 4019 5175 4019
Size: 1156 4806
ApoI r'AATT_y
Cuts at: 1493 2880 3271 3497 3649 5758 1493
Size: 1387 391 226 152 2109 1697
Fragments arranged by size:
2109 1697 1387 391 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4516 160
Size: 872 3484 1606
Fragments arranged by size:
3484 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5962
AvaI C'yCGr_G
Cuts at: 1870 1943 2421 3287 1870
Size: 73 478 866 4545
Fragments arranged by size:
4545 866 478 73
AvrII C'CTAG_G
Cuts at: 2686 2686
Size: 5962
BamHI G'GATC_C
Cuts at: 2012 3292 3659 2012
Size: 1280 367 4315
Fragments arranged by size:
4315 1280 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2156 3283 4738 618
Size: 325 217 627 369 1127 1455 1842
Fragments arranged by size:
1842 1455 1127 627 369 325 217
BanII G_rGCy'C
Cuts at: 1391 2602 3281 1391
Size: 1211 679 4072
Fragments arranged by size:
4072 1211 679
BbeI G_GCGC'C
Cuts at: 2160 2160
Size: 5962
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4991
BbvCI CC'TCA_GC
Cuts at: 2665 2665
Size: 5962
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4153 4187 1903
Size: 34 2216 34 3678
Fragments arranged by size:
3678 2216 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3854 5064 3854
Size: 1210 4752
BclI T'GATC_A
Cuts at: 1548 2779 1548
Size: 1231 4731
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4573 136
Size: 107 122 71 631 605 2901 1525
Fragments arranged by size:
2901 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2741 2944 5957 2741
Size: 203 3013 2746
Fragments arranged by size:
3013 2746 203
BlpI GC'TnA_GC
Cuts at: 3379 3379
Size: 5962
Bme1580I G_kGCm'C
Cuts at: 1790 2422 4023 5179 1790
Size: 632 1601 1156 2573
Fragments arranged by size:
2573 1601 1156 632
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5962
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3693 4651 442
Size: 416 53 83 399 24 2276 958 1753
Fragments arranged by size:
2276 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5962
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2072 2586 2949 4622 1239
Size: 833 514 363 1673 2579
Fragments arranged by size:
2579 1673 833 514 363
Bpu10I CC'TnA_GC
Cuts at: 2665 2665
Size: 5962
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2147 3026 4087 4952 5250 2147
Size: 879 1061 865 298 2859
Fragments arranged by size:
2859 1061 879 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2832 4625 882
Size: 617 1333 1793 2219
Fragments arranged by size:
2219 1793 1333 617
BsaAI yAC'GTr
Cuts at: 493 1204 2489 3753 493
Size: 711 1285 1264 2702
Fragments arranged by size:
2702 1285 1264 711
BsaBI GATnn'nnATC
Cuts at: 3000 5956 3000
Size: 2956 3006
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2157 3769 4151 4786
Size: 53 83 186 936 624 1612 382 635
Cuts at: 4786 275
Size: 1451
Fragments arranged by size:
1612 1451 936 635 624 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4395 4793 5067 5214 5625 4395
Size: 398 274 147 411 4732
Fragments arranged by size:
4732 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5737
Fragments arranged by size:
5737 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2092 3065 1186
Size: 906 973 4083
Fragments arranged by size:
4083 973 906
BseYI C'CCAG_C
Cuts at: 5165 5661 5165
Size: 496 5466
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2526 2923 1163
Size: 573 790 397 4202
Fragments arranged by size:
4202 790 573 397
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3412 4173 5201 1540
Size: 275 186 1411 761 1028 2301
Fragments arranged by size:
2301 1411 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4023 4108 5179 4023
Size: 85 1071 4806
Fragments arranged by size:
4806 1071 85
BsmI GAATG_Cn'
Cuts at: 2815 2919 3473 3566 2815
Size: 104 554 93 5211
Fragments arranged by size:
5211 554 104 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2422 2602 3281 4023 4108 5179 1391
Size: 399 632 180 679 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 679 632 399 180 85
BspEI T'CCGG_A
Cuts at: 5625 5625
Size: 5962
BspHI T'CATG_A
Cuts at: 2455 2860 3851 5581 2455
Size: 405 991 1730 2836
Fragments arranged by size:
2836 1730 991 405
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2065 3317 844
Size: 1221 1252 3489
Fragments arranged by size:
3489 1252 1221
BsrBI CCG'CTC
Cuts at: 1394 1823 3849 1394
Size: 429 2026 3507
Fragments arranged by size:
3507 2026 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4457 4631 65
Size: 1183 3209 174 1396
Fragments arranged by size:
3209 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4606 4793 1700
Size: 110 2796 187 2869
Fragments arranged by size:
2869 2796 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5962
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5962
BssSI C'ACGA_G
Cuts at: 4022 5034 4022
Size: 1012 4950
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2173 794
Size: 1379 4583
BstBI TT'CG_AA
Cuts at: 4766 4766
Size: 5962
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5962
BstYI r'GATC_y
Cuts at: 1972 2012 2741 2944 3292 3659 4044 4061 5513
Size: 40 729 203 348 367 385 17 1452
Cuts at: 5513 5599 5611 5957 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 729 385 367 348 346 203
86 40 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4471
Fragments arranged by size:
4471 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5223
Fragments arranged by size:
5223 683 56
BtsI GCAGTG_nn'
Cuts at: 2808 3478 4272 4292 2808
Size: 670 794 20 4478
Fragments arranged by size:
4478 794 670 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5962
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5927
DraI TTT'AAA
Cuts at: 2524 3266 2524
Size: 742 5220
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4969 809
Size: 507 3653 1802
Fragments arranged by size:
3653 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3409 4298 5927
Size: 54 1425 50 275 18 1579 889 1629
Cuts at: 5927 8
Size: 43
Fragments arranged by size:
1629 1579 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3409 1537
Size: 275 1597 4090
Fragments arranged by size:
4090 1597 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3009 3892 1353
Size: 1656 883 3423
Fragments arranged by size:
3423 1656 883
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2095 4549 4923 5069 387
Size: 1064 138 506 2454 374 146 1280
Fragments arranged by size:
2454 1280 1064 506 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2072 2586 2949 4025 4622 5409 5677
Size: 393 440 514 363 1076 597 787 268
Cuts at: 5677 1239
Size: 1524
Fragments arranged by size:
1524 1076 787 597 514 440 393 363
268
EcoNI CCTnn'n_nnAGG
Cuts at: 3108 3232 3108
Size: 124 5838
EcoO109I rG'GnC_Cy
Cuts at: 2437 2437
Size: 5962
EcoRI G'AATT_C
Cuts at: 2880 3271 3649 2880
Size: 391 378 5193
Fragments arranged by size:
5193 391 378
EcoRV GAT'ATC
Cuts at: 3242 3242
Size: 5962
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2188 2188
Size: 5962
FspI TGC'GCA
Cuts at: 3159 4468 3159
Size: 1309 4653
HaeII r_GCGC'y
Cuts at: 1678 2160 2243 5109 1678
Size: 482 83 2866 2531
Fragments arranged by size:
2866 2531 482 83
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2829 2861 3102 3134 3226
Size: 32 135 32 1257 32 241 32 92
Cuts at: 3226 3258 4603 4635 4677 4709 1373
Size: 32 1345 32 42 32 2626
Fragments arranged by size:
2626 1345 1257 241 135 92 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3306 3552 669
Size: 988 281 1368 246 3079
Fragments arranged by size:
3079 1368 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5962
HpaI GTT'AAC
Cuts at: 3552 3552
Size: 5962
KasI G'GCGC_C
Cuts at: 2156 2156
Size: 5962
KpnI G_GTAC'C
Cuts at: 3287 3287
Size: 5962
MfeI C'AATT_G
Cuts at: 3561 3561
Size: 5962
MluI A'CGCG_T
Cuts at: 5838 5838
Size: 5962
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5076 5260 1305
Size: 3771 184 2007
Fragments arranged by size:
3771 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4140
Fragments arranged by size:
4140 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2402 2966 3920 4279 4438 518
Size: 648 1236 564 954 359 159 2042
Fragments arranged by size:
2042 1236 954 648 564 359 159
MspA1I CmG'CkG
Cuts at: 1606 2669 3220 3378 4055 5203 5448 1606
Size: 1063 551 158 677 1148 245 2120
Fragments arranged by size:
2120 1148 1063 677 551 245 158
NarI GG'CG_CC
Cuts at: 2157 2157
Size: 5962
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4471
Fragments arranged by size:
4471 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5962
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5962
NotI GC'GGCC_GC
Cuts at: 3409 3409
Size: 5962
NspI r_CATG'y
Cuts at: 1132 1229 2857 3320 4865 1132
Size: 97 1628 463 1545 2229
Fragments arranged by size:
2229 1628 1545 463 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5962
PciI A'CATG_T
Cuts at: 4861 4861
Size: 5962
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5962
PfoI T'CCnGG_A
Cuts at: 2343 2343
Size: 5962
PmeI GTTT'AAAC
Cuts at: 3266 3266
Size: 5962
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4031 4063 5544 5576 4031
Size: 32 1481 32 4417
Fragments arranged by size:
4417 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5962
PsiI TTA'TAA
Cuts at: 3532 3532
Size: 5962
PstI C_TGCA'G
Cuts at: 830 2646 3314 830
Size: 1816 668 3478
Fragments arranged by size:
3478 1816 668
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5962
PvuII CAG'CTG
Cuts at: 2669 2669
Size: 5962
SalI G'TCGA_C
Cuts at: 1936 3304 1936
Size: 1368 4594
SbfI CC_TGCA'GG
Cuts at: 3314 3314
Size: 5962
ScaI AGT'ACT
Cuts at: 4210 5922 4210
Size: 1712 4250
SfcI C'TryA_G
Cuts at: 826 1045 2642 3310 4445 5126 5317 826
Size: 219 1597 668 1135 681 191 1471
Fragments arranged by size:
1597 1471 1135 681 668 219 191
SfoI GGC'GCC
Cuts at: 2158 2158
Size: 5962
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5962
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5962
SmaI CCC'GGG
Cuts at: 1872 3289 1872
Size: 1417 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2162 3005 4066 4967 5229 820
Size: 197 926 219 843 1061 901 262 1553
Fragments arranged by size:
1553 1061 926 901 843 262 219 197
SnaBI TAC'GTA
Cuts at: 493 3753 493
Size: 3260 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5962
SphI G_CATG'C
Cuts at: 1132 2857 3320 1132
Size: 1725 463 3774
Fragments arranged by size:
3774 1725 463
SspI AAT'ATT
Cuts at: 5 52 3886 5
Size: 47 3834 2081
Fragments arranged by size:
3834 2081 47
StuI AGG'CCT
Cuts at: 1517 2057 1517
Size: 540 5422
StyI C'CwwG_G
Cuts at: 513 1065 2004 2686 3401 513
Size: 552 939 682 715 3074
Fragments arranged by size:
3074 939 715 682 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3989 4006 4159 4344 4789 325
Size: 1255 2409 17 153 185 445 1498
Fragments arranged by size:
2409 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4208 5920 96
Size: 274 80 33 51 1443 2231 1712 138
Fragments arranged by size:
2231 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5922 5954 5922
Size: 32 5930
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5962
XbaI T'CTAG_A
Cuts at: 3298 3298
Size: 5962
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2800 2800
Size: 5962
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5962
XmaI C'CCGG_G
Cuts at: 1870 3287 1870
Size: 1417 4545
XmnI GAAnn'nnTTC
Cuts at: 4091 4091
Size: 5962
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3770 276
Size: 53 83 186 936 2236 2468
Fragments arranged by size:
2468 2236 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI FseI
FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SrfI SwaI