Restriction map for
pFN21AB3634
(Circular) (Six-base) MAPSORT of: pFN21AB3634.seq Check: 2341 from: 1 to: 5704
pFN21AB3634 5704 bp
With 182 enzymes: SgfI *
October 20, 2009 10:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3514 278
Size: 53 83 186 936 1978 2468
Fragments arranged by size:
2468 1978 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3047 1937
Size: 1110 4594
Acc65I G'GTAC_C
Cuts at: 3025 3025
Size: 5704
AclI AA'CG_TT
Cuts at: 1563 3831 4204 1563
Size: 2268 373 3063
Fragments arranged by size:
3063 2268 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3767 5151 5419 1632
Size: 2135 1384 268 1917
Fragments arranged by size:
2135 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5507
AflIII A'CryG_T
Cuts at: 1203 4603 5580 1203
Size: 3400 977 1327
Fragments arranged by size:
3400 1327 977
AgeI A'CCGG_T
Cuts at: 4535 4535
Size: 5704
AhdI GACnn_n'nnGTC
Cuts at: 4433 4433
Size: 5704
AleI CACnn'nnGTG
Cuts at: 1166 2328 2435 1166
Size: 1162 107 4435
Fragments arranged by size:
4435 1162 107
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5704
AlwNI CAG_nnn'CTG
Cuts at: 3447 5019 3447
Size: 1572 4132
ApaLI G'TGCA_C
Cuts at: 3761 4917 3761
Size: 1156 4548
ApoI r'AATT_y
Cuts at: 1493 3013 3239 3391 5500 1493
Size: 1520 226 152 2109 1697
Fragments arranged by size:
2109 1697 1520 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2901 4258 160
Size: 872 1869 1357 1606
Fragments arranged by size:
1869 1606 1357 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5704
AvaI C'yCGr_G
Cuts at: 1870 1943 3029 1870
Size: 73 1086 4545
Fragments arranged by size:
4545 1086 73
BamHI G'GATC_C
Cuts at: 3034 3401 3034
Size: 367 5337
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2645 3025 4480 618
Size: 325 217 627 858 380 1455 1842
Fragments arranged by size:
1842 1455 858 627 380 325 217
BanII G_rGCy'C
Cuts at: 1391 3023 1391
Size: 1632 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4733
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3895 3929 1903
Size: 34 1958 34 3678
Fragments arranged by size:
3678 1958 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3596 4806 3596
Size: 1210 4494
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5704
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4315 136
Size: 107 122 71 631 605 2643 1525
Fragments arranged by size:
2643 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2200 5699 2200
Size: 3499 2205
BlpI GC'TnA_GC
Cuts at: 2082 2241 3121 2082
Size: 159 880 4665
Fragments arranged by size:
4665 880 159
Bme1580I G_kGCm'C
Cuts at: 1790 3765 4921 1790
Size: 1975 1156 2573
Fragments arranged by size:
2573 1975 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5704
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3435 4393 442
Size: 416 53 83 399 24 2018 958 1753
Fragments arranged by size:
2018 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5704
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2355 2868 4364 1239
Size: 1116 513 1496 2579
Fragments arranged by size:
2579 1496 1116 513
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2405 3829 4694 4992 2405
Size: 1424 865 298 3117
Fragments arranged by size:
3117 1424 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4367 882
Size: 617 2868 2219
Fragments arranged by size:
2868 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3495 493
Size: 711 2291 2702
Fragments arranged by size:
2702 2291 711
BsaBI GATnn'nnATC
Cuts at: 5698 5698
Size: 5704
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3511 3893 4528 275
Size: 53 83 186 936 1978 382 635 1451
Fragments arranged by size:
1978 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4137 4535 4809 4956 5367 4137
Size: 398 274 147 411 4474
Fragments arranged by size:
4474 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2139 2169 1178
Size: 30 165 30 736 30 4713
Fragments arranged by size:
4713 736 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5704
BseYI C'CCAG_C
Cuts at: 2867 4907 5403 2867
Size: 2040 496 3168
Fragments arranged by size:
3168 2040 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5131
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3154 3915 4943 1540
Size: 275 186 1153 761 1028 2301
Fragments arranged by size:
2301 1153 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3765 3850 4921 3765
Size: 85 1071 4548
Fragments arranged by size:
4548 1071 85
BsmI GAATG_Cn'
Cuts at: 3215 3308 3215
Size: 93 5611
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3023 3765 3850 4921 1391
Size: 399 1233 742 85 1071 2174
Fragments arranged by size:
2174 1233 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5367 5367
Size: 5704
BspHI T'CATG_A
Cuts at: 3593 5323 3593
Size: 1730 3974
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3059 844
Size: 2215 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2015 3591 1394
Size: 429 192 1576 3507
Fragments arranged by size:
3507 1576 429 192
BsrDI GCAATG_nn'
Cuts at: 65 1248 2048 2409 4199 4373 65
Size: 1183 800 361 1790 174 1396
Fragments arranged by size:
1790 1396 1183 800 361 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4348 4535 1700
Size: 110 2538 187 2869
Fragments arranged by size:
2869 2538 187 110
BsrGI T'GTAC_A
Cuts at: 96 2253 2468 96
Size: 2157 215 3332
Fragments arranged by size:
3332 2157 215
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5704
BssSI C'ACGA_G
Cuts at: 3764 4776 3764
Size: 1012 4692
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2459 2741 794
Size: 1665 282 3757
Fragments arranged by size:
3757 1665 282
BstBI TT'CG_AA
Cuts at: 4508 4508
Size: 5704
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5704
BstXI CCAn_nnnn'nTGG
Cuts at: 2656 2656
Size: 5704
BstYI r'GATC_y
Cuts at: 1972 2200 3034 3401 3786 3803 5255 5341 5353
Size: 228 834 367 385 17 1452 86 12
Cuts at: 5353 5699 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 834 385 367 346 228 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2509 513
Size: 552 1444 3708
Fragments arranged by size:
3708 1444 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4965
Fragments arranged by size:
4965 683 56
BtsI GCAGTG_nn'
Cuts at: 3220 4014 4034 3220
Size: 794 20 4890
Fragments arranged by size:
4890 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5704
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2125 2160 529
Size: 35 1561 35 4073
Fragments arranged by size:
4073 1561 35 35
DraI TTT'AAA
Cuts at: 2118 3008 2118
Size: 890 4814
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4711 809
Size: 507 3395 1802
Fragments arranged by size:
3395 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3151 4040 5669
Size: 54 1425 50 275 18 1321 889 1629
Cuts at: 5669 8
Size: 43
Fragments arranged by size:
1629 1425 1321 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3151 1537
Size: 275 1339 4090
Fragments arranged by size:
4090 1339 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2675 3634 1353
Size: 1322 959 3423
Fragments arranged by size:
3423 1322 959
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4291 4665 4811 387
Size: 1064 138 2702 374 146 1280
Fragments arranged by size:
2702 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2355 2868 3767 4364 5151 5419 1239
Size: 393 723 513 899 597 787 268 1524
Fragments arranged by size:
1524 899 787 723 597 513 393 268
EcoRI G'AATT_C
Cuts at: 3013 3391 3013
Size: 378 5326
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2282 2282
Size: 5704
FspI TGC'GCA
Cuts at: 4210 4210
Size: 5704
HaeII r_GCGC'y
Cuts at: 1678 4851 1678
Size: 3173 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4345 4377 4419 4451 1373
Size: 32 135 32 2773 32 42 32 2626
Fragments arranged by size:
2773 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3048 3294 669
Size: 988 281 1110 246 3079
Fragments arranged by size:
3079 1110 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5704
HpaI GTT'AAC
Cuts at: 3294 3294
Size: 5704
KpnI G_GTAC'C
Cuts at: 3029 3029
Size: 5704
MfeI C'AATT_G
Cuts at: 2889 3303 2889
Size: 414 5290
MluI A'CGCG_T
Cuts at: 5580 5580
Size: 5704
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2773 2818 2881 4818 5002 1305
Size: 1468 45 63 1937 184 2007
Fragments arranged by size:
2007 1937 1468 184 63 45
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3882
Fragments arranged by size:
3882 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2091 2328 2435 3662 4021 4180 518
Size: 648 925 237 107 1227 359 159 2042
Fragments arranged by size:
2042 1227 925 648 359 237 159 107
MspA1I CmG'CkG
Cuts at: 1606 3120 3797 4945 5190 1606
Size: 1514 677 1148 245 2120
Fragments arranged by size:
2120 1514 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5152
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5704
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5704
NotI GC'GGCC_GC
Cuts at: 3151 3151
Size: 5704
NspI r_CATG'y
Cuts at: 1132 1229 2090 2732 2788 3062 4607 1132
Size: 97 861 642 56 274 1545 2229
Fragments arranged by size:
2229 1545 861 642 274 97 56
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5704
PciI A'CATG_T
Cuts at: 4603 4603
Size: 5704
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5704
PmeI GTTT'AAAC
Cuts at: 3008 3008
Size: 5704
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3773 3805 5286 5318 3773
Size: 32 1481 32 4159
Fragments arranged by size:
4159 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5704
PsiI TTA'TAA
Cuts at: 3274 3274
Size: 5704
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2539 2571 2539
Size: 32 5672
PstI C_TGCA'G
Cuts at: 830 2134 2533 2737 3056 830
Size: 1304 399 204 319 3478
Fragments arranged by size:
3478 1304 399 319 204
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5704
SalI G'TCGA_C
Cuts at: 1936 3046 1936
Size: 1110 4594
SbfI CC_TGCA'GG
Cuts at: 3056 3056
Size: 5704
ScaI AGT'ACT
Cuts at: 2575 3952 5664 2575
Size: 1377 1712 2615
Fragments arranged by size:
2615 1712 1377
SfcI C'TryA_G
Cuts at: 826 1045 2130 2529 2733 3052 4187 4868 5059
Size: 219 1085 399 204 319 1135 681 191
Cuts at: 5059 826
Size: 1471
Fragments arranged by size:
1471 1135 1085 681 399 319 219 204
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5704
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5704
SmaI CCC'GGG
Cuts at: 1872 3031 1872
Size: 1159 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2384 3808 4709 4971 820
Size: 197 926 441 1424 901 262 1553
Fragments arranged by size:
1553 1424 926 901 441 262 197
SnaBI TAC'GTA
Cuts at: 493 3495 493
Size: 3002 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5704
SphI G_CATG'C
Cuts at: 1132 2090 3062 1132
Size: 958 972 3774
Fragments arranged by size:
3774 972 958
SspI AAT'ATT
Cuts at: 5 52 3628 5
Size: 47 3576 2081
Fragments arranged by size:
3576 2081 47
StuI AGG'CCT
Cuts at: 1517 2262 1517
Size: 745 4959
StyI C'CwwG_G
Cuts at: 513 1065 2143 3143 513
Size: 552 1078 1000 3074
Fragments arranged by size:
3074 1078 1000 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2122 3731 3748 3901 4086 4531 325
Size: 1255 542 1609 17 153 185 445 1498
Fragments arranged by size:
1609 1498 1255 542 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2253 2468 2573
Size: 274 80 33 51 1443 276 215 105
Cuts at: 2573 3950 5662 96
Size: 1377 1712 138
Fragments arranged by size:
1712 1443 1377 276 274 215 138 105
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2139 2171 5664 5696 2139
Size: 32 3493 32 2147
Fragments arranged by size:
3493 2147 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5704
XbaI T'CTAG_A
Cuts at: 3040 3040
Size: 5704
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2864 2864
Size: 5704
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5704
XmaI C'CCGG_G
Cuts at: 1870 3029 1870
Size: 1159 4545
XmnI GAAnn'nnTTC
Cuts at: 2309 3833 2309
Size: 1524 4180
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3512 276
Size: 53 83 186 936 1978 2468
Fragments arranged by size:
2468 1978 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PsrI PstI PvuI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI