Restriction map for
pFN21AB3630
(Circular) (Six-base) MAPSORT of: pFN21AB3630.seq Check: 8203 from: 1 to: 5284
pFN21AB3630 5284 bp
With 182 enzymes: SgfI *
October 20, 2009 10:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2149 2149
Size: 5284
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3094 278
Size: 53 83 186 936 1558 2468
Fragments arranged by size:
2468 1558 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2103 2627 1937
Size: 166 524 4594
Fragments arranged by size:
4594 524 166
Acc65I G'GTAC_C
Cuts at: 2605 2605
Size: 5284
AclI AA'CG_TT
Cuts at: 1563 3411 3784 1563
Size: 1848 373 3063
Fragments arranged by size:
3063 1848 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2062 3347 4731 4999 1632
Size: 430 1285 1384 268 1917
Fragments arranged by size:
1917 1384 1285 430 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5087
AflIII A'CryG_T
Cuts at: 1203 4183 5160 1203
Size: 2980 977 1327
Fragments arranged by size:
2980 1327 977
AgeI A'CCGG_T
Cuts at: 4115 4115
Size: 5284
AhdI GACnn_n'nnGTC
Cuts at: 4013 4013
Size: 5284
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5284
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5284
AlwNI CAG_nnn'CTG
Cuts at: 3027 4599 3027
Size: 1572 3712
ApaLI G'TGCA_C
Cuts at: 3341 4497 3341
Size: 1156 4128
ApoI r'AATT_y
Cuts at: 1493 2593 2819 2971 5080 1493
Size: 1100 226 152 2109 1697
Fragments arranged by size:
2109 1697 1100 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3838 160
Size: 872 2806 1606
Fragments arranged by size:
2806 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5284
AvaI C'yCGr_G
Cuts at: 1870 1943 2529 2609 1870
Size: 73 586 80 4545
Fragments arranged by size:
4545 586 80 73
BamHI G'GATC_C
Cuts at: 2614 2981 2614
Size: 367 4917
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2045 2605 4060 618
Size: 325 217 627 258 560 1455 1842
Fragments arranged by size:
1842 1455 627 560 325 258 217
BanII G_rGCy'C
Cuts at: 1391 2418 2603 1391
Size: 1027 185 4072
Fragments arranged by size:
4072 1027 185
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2063 2068 2202 928
Size: 971 164 5 134 4010
Fragments arranged by size:
4010 971 164 134 5
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3475 3509 1903
Size: 34 1538 34 3678
Fragments arranged by size:
3678 1538 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3176 4386 3176
Size: 1210 4074
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5284
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2531 3895 136
Size: 107 122 71 631 605 859 1364 1525
Fragments arranged by size:
1525 1364 859 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5279 5279
Size: 5284
BlpI GC'TnA_GC
Cuts at: 2482 2701 2482
Size: 219 5065
Bme1580I G_kGCm'C
Cuts at: 1790 2337 3345 4501 1790
Size: 547 1008 1156 2573
Fragments arranged by size:
2573 1156 1008 547
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5284
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3015 3973 442
Size: 416 53 83 399 24 1598 958 1753
Fragments arranged by size:
1753 1598 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5284
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2525 3944 1239
Size: 1286 1419 2579
Fragments arranged by size:
2579 1419 1286
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2495 3409 4274 4572 2495
Size: 914 865 298 3207
Fragments arranged by size:
3207 914 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3947 882
Size: 617 2448 2219
Fragments arranged by size:
2448 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3075 493
Size: 711 1871 2702
Fragments arranged by size:
2702 1871 711
BsaBI GATnn'nnATC
Cuts at: 5278 5278
Size: 5284
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3091 3473 4108 275
Size: 53 83 186 936 1558 382 635 1451
Fragments arranged by size:
1558 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2095 2451 3717 4115 4389 4536 4947 2095
Size: 356 1266 398 274 147 411 2432
Fragments arranged by size:
2432 1266 411 398 356 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2557 2587 1178
Size: 30 165 30 1154 30 3875
Fragments arranged by size:
3875 1154 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2057 1186
Size: 871 4413
BseYI C'CCAG_C
Cuts at: 4487 4983 4487
Size: 496 4788
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4711
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2734 3495 4523 1540
Size: 275 186 733 761 1028 2301
Fragments arranged by size:
2301 1028 761 733 275 186
BsiHKAI G_wGCw'C
Cuts at: 2382 2483 3345 3430 4501 2382
Size: 101 862 85 1071 3165
Fragments arranged by size:
3165 1071 862 101 85
BsmI GAATG_Cn'
Cuts at: 2795 2888 2795
Size: 93 5191
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2337 2382 2418 2483 2603 3345 3430
Size: 399 547 45 36 65 120 742 85
Cuts at: 3430 4501 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 547 399 120 85 65
45 36
BspEI T'CCGG_A
Cuts at: 2095 4947 2095
Size: 2852 2432
BspHI T'CATG_A
Cuts at: 3173 4903 3173
Size: 1730 3554
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2069 2149 2639 844
Size: 1225 80 490 3489
Fragments arranged by size:
3489 1225 490 80
BsrBI CCG'CTC
Cuts at: 1394 1823 3171 1394
Size: 429 1348 3507
Fragments arranged by size:
3507 1348 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3779 3953 65
Size: 1183 2531 174 1396
Fragments arranged by size:
2531 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3928 4115 1700
Size: 110 2118 187 2869
Fragments arranged by size:
2869 2118 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5284
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5284
BssSI C'ACGA_G
Cuts at: 3344 4356 3344
Size: 1012 4272
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5284
BstBI TT'CG_AA
Cuts at: 4088 4088
Size: 5284
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5284
BstXI CCAn_nnnn'nTGG
Cuts at: 2344 2344
Size: 5284
BstYI r'GATC_y
Cuts at: 1972 2614 2981 3366 3383 4835 4921 4933 5279
Size: 642 367 385 17 1452 86 12 346
Cuts at: 5279 1972
Size: 1977
Fragments arranged by size:
1977 1452 642 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2366 2366
Size: 5284
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3793
Fragments arranged by size:
3793 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4545
Fragments arranged by size:
4545 683 56
BtsI GCAGTG_nn'
Cuts at: 2800 3594 3614 2800
Size: 794 20 4470
Fragments arranged by size:
4470 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5284
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5249
DraI TTT'AAA
Cuts at: 2588 2588
Size: 5284
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2237 4291 809
Size: 507 921 2054 1802
Fragments arranged by size:
2054 1802 921 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2397 2523 2731
Size: 54 1425 50 275 18 567 126 208
Cuts at: 2731 3620 5249 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 567 275 208 126 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2731 1537
Size: 275 919 4090
Fragments arranged by size:
4090 919 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3214 1353
Size: 1861 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3871 4245 4391 387
Size: 1064 138 2282 374 146 1280
Fragments arranged by size:
2282 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2062 2525 3347 3944 4731 4999 1239
Size: 393 430 463 822 597 787 268 1524
Fragments arranged by size:
1524 822 787 597 463 430 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2362 2362
Size: 5284
EcoRI G'AATT_C
Cuts at: 2593 2971 2593
Size: 378 4906
FspI TGC'GCA
Cuts at: 3790 3790
Size: 5284
HaeII r_GCGC'y
Cuts at: 1678 4431 1678
Size: 2753 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2512 2544 2557 2589 3925
Size: 32 135 32 940 32 13 32 1336
Cuts at: 3925 3957 3999 4031 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1336 940 135 42 32 32 32
32 32 32 13
HincII GTy'rAC
Cuts at: 669 1657 1938 2628 2874 669
Size: 988 281 690 246 3079
Fragments arranged by size:
3079 988 690 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5284
HpaI GTT'AAC
Cuts at: 2874 2874
Size: 5284
KpnI G_GTAC'C
Cuts at: 2609 2609
Size: 5284
MfeI C'AATT_G
Cuts at: 2883 2883
Size: 5284
MluI A'CGCG_T
Cuts at: 5160 5160
Size: 5284
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2543 4398 4582 1305
Size: 1238 1855 184 2007
Fragments arranged by size:
2007 1855 1238 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2399 2525 10
Size: 54 1425 343 567 126 2769
Fragments arranged by size:
2769 1425 567 343 126 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2144 2246 2345 3242 3601 3760 518
Size: 648 978 102 99 897 359 159 2042
Fragments arranged by size:
2042 978 897 648 359 159 102 99
MspA1I CmG'CkG
Cuts at: 1606 2700 3377 4525 4770 1606
Size: 1094 677 1148 245 2120
Fragments arranged by size:
2120 1148 1094 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3793
Fragments arranged by size:
3793 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5284
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5284
NotI GC'GGCC_GC
Cuts at: 2731 2731
Size: 5284
NspI r_CATG'y
Cuts at: 1132 1229 2642 4187 1132
Size: 97 1413 1545 2229
Fragments arranged by size:
2229 1545 1413 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5284
PciI A'CATG_T
Cuts at: 4183 4183
Size: 5284
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5284
PmeI GTTT'AAAC
Cuts at: 2588 2588
Size: 5284
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3353 3385 4866 4898 3353
Size: 32 1481 32 3739
Fragments arranged by size:
3739 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2362 2362
Size: 5284
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5284
PsiI TTA'TAA
Cuts at: 2854 2854
Size: 5284
PstI C_TGCA'G
Cuts at: 830 2636 830
Size: 1806 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5284
SalI G'TCGA_C
Cuts at: 1936 2626 1936
Size: 690 4594
SbfI CC_TGCA'GG
Cuts at: 2636 2636
Size: 5284
ScaI AGT'ACT
Cuts at: 3532 5244 3532
Size: 1712 3572
SfcI C'TryA_G
Cuts at: 826 1045 2632 3767 4448 4639 826
Size: 219 1587 1135 681 191 1471
Fragments arranged by size:
1587 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5284
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5284
SmaI CCC'GGG
Cuts at: 1872 2611 1872
Size: 739 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2474 3388 4289 4551 820
Size: 197 926 531 914 901 262 1553
Fragments arranged by size:
1553 926 914 901 531 262 197
SnaBI TAC'GTA
Cuts at: 493 3075 493
Size: 2582 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5284
SphI G_CATG'C
Cuts at: 1132 2642 1132
Size: 1510 3774
SspI AAT'ATT
Cuts at: 5 52 3208 5
Size: 47 3156 2081
Fragments arranged by size:
3156 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5284
StyI C'CwwG_G
Cuts at: 513 1065 2004 2493 2723 513
Size: 552 939 489 230 3074
Fragments arranged by size:
3074 939 552 489 230
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2412 3311 3328 3481 3666 4111 325
Size: 1255 832 899 17 153 185 445 1498
Fragments arranged by size:
1498 1255 899 832 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3530 5242 96
Size: 274 80 33 51 1443 1553 1712 138
Fragments arranged by size:
1712 1553 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5244 5276 5244
Size: 32 5252
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5284
XbaI T'CTAG_A
Cuts at: 2620 2620
Size: 5284
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2344 2392 2344
Size: 48 5236
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5284
XmaI C'CCGG_G
Cuts at: 1870 2609 1870
Size: 739 4545
XmnI GAAnn'nnTTC
Cuts at: 2286 2318 3413 2286
Size: 32 1095 4157
Fragments arranged by size:
4157 1095 32
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3092 276
Size: 53 83 186 936 1558 2468
Fragments arranged by size:
2468 1558 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI