Restriction map for
pFN21AB3626
(Circular) (Six-base) MAPSORT of: pFN21AB3626.seq Check: 79 from: 1 to: 8167
pFN21AB3626 8167 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3452 3454 4705 3452
Size: 2 1251 6914
Fragments arranged by size:
6914 1251 2
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5196 5977 278
Size: 53 83 186 936 3660 781 2468
Fragments arranged by size:
3660 2468 936 781 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3705 5510 1937
Size: 1768 1805 4594
Fragments arranged by size:
4594 1805 1768
Acc65I G'GTAC_C
Cuts at: 5488 5488
Size: 8167
AclI AA'CG_TT
Cuts at: 1563 2931 6294 6667 1563
Size: 1368 3363 373 3063
Fragments arranged by size:
3363 3063 1368 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2822 3047 3206 3861 4614 4754 6230 7614
Size: 1190 225 159 655 753 140 1476 1384
Cuts at: 7614 7882 1632
Size: 268 1917
Fragments arranged by size:
1917 1476 1384 1190 753 655 268 225
159 140
AfeI AGC'GCT
Cuts at: 4622 4622
Size: 8167
AflII C'TTAA_G
Cuts at: 820 1017 4789 4895 820
Size: 197 3772 106 4092
Fragments arranged by size:
4092 3772 197 106
AflIII A'CryG_T
Cuts at: 1203 7066 8043 1203
Size: 5863 977 1327
Fragments arranged by size:
5863 1327 977
AgeI A'CCGG_T
Cuts at: 6998 6998
Size: 8167
AhdI GACnn_n'nnGTC
Cuts at: 2305 5197 6896 2305
Size: 2892 1699 3576
Fragments arranged by size:
3576 2892 1699
AleI CACnn'nnGTG
Cuts at: 1166 3281 1166
Size: 2115 6052
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2221 1184
Size: 1037 7130
AlwNI CAG_nnn'CTG
Cuts at: 3595 4592 5910 7482 3595
Size: 997 1318 1572 4280
Fragments arranged by size:
4280 1572 1318 997
ApaLI G'TGCA_C
Cuts at: 6224 7380 6224
Size: 1156 7011
ApoI r'AATT_y
Cuts at: 1493 2753 4076 4874 5174 5402 5476 5702 5854
Size: 1260 1323 798 300 228 74 226 152
Cuts at: 5854 7963 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1323 1260 798 300 228 226
152 74
AseI AT'TA_AT
Cuts at: 160 1032 4539 6721 160
Size: 872 3507 2182 1606
Fragments arranged by size:
3507 2182 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8167
AvaI C'yCGr_G
Cuts at: 1870 1943 3648 5492 1870
Size: 73 1705 1844 4545
Fragments arranged by size:
4545 1844 1705 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2456 2489 2456
Size: 33 8134
BamHI G'GATC_C
Cuts at: 3735 4635 5497 5864 3735
Size: 900 862 367 6038
Fragments arranged by size:
6038 900 862 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3473 5488 6943 618
Size: 325 217 627 1686 2015 1455 1842
Fragments arranged by size:
2015 1842 1686 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2069 2145 4677 5486 1391
Size: 678 76 2532 809 4072
Fragments arranged by size:
4072 2532 809 678 76
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2081 4848 928
Size: 971 182 2767 4247
Fragments arranged by size:
4247 2767 971 182
BbvCI CC'TCA_GC
Cuts at: 3398 3398
Size: 8167
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 6358 6392 1903
Size: 34 50 34 4337 34 3678
Fragments arranged by size:
4337 3678 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6059 7269 6059
Size: 1210 6957
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8167
BfrBI ATG'CAT
Cuts at: 4260 4260
Size: 8167
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2149 6778 136
Size: 107 122 71 631 605 477 4629 1525
Fragments arranged by size:
4629 1525 631 605 477 122 107 71
BglII A'GATC_T
Cuts at: 4785 8162 4785
Size: 3377 4790
BlpI GC'TnA_GC
Cuts at: 5584 5584
Size: 8167
Bme1580I G_kGCm'C
Cuts at: 1790 3478 4108 5354 6228 7384 1790
Size: 1688 630 1246 874 1156 2573
Fragments arranged by size:
2573 1688 1246 1156 874 630
BmgBI CAC'GTC
Cuts at: 1318 3283 3985 1318
Size: 1965 702 5500
Fragments arranged by size:
5500 1965 702
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2967 5898 6856
Size: 416 53 83 399 24 1550 2931 958
Cuts at: 6856 442
Size: 1753
Fragments arranged by size:
2931 1753 1550 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8167
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2111 2489 5136 6827 1239
Size: 872 378 2647 1691 2579
Fragments arranged by size:
2647 2579 1691 872 378
Bpu10I CC'TnA_GC
Cuts at: 2100 2484 3398 4109 2100
Size: 384 914 711 6158
Fragments arranged by size:
6158 914 711 384
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3566 3725 6292 7157 7455 3566
Size: 159 2567 865 298 4278
Fragments arranged by size:
4278 2567 865 298 159
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2520 3558 3943 6830 882
Size: 617 1021 1038 385 2887 2219
Fragments arranged by size:
2887 2219 1038 1021 617 385
BsaAI yAC'GTr
Cuts at: 493 1204 3394 5958 493
Size: 711 2190 2564 2702
Fragments arranged by size:
2702 2564 2190 711
BsaBI GATnn'nnATC
Cuts at: 3261 8161 3261
Size: 4900 3267
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2386 3969 5193 5974
Size: 53 83 186 936 853 1583 1224 781
Cuts at: 5974 6356 6991 275
Size: 382 635 1451
Fragments arranged by size:
1583 1451 1224 936 853 781 635 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 2325 4044 6600 6998 7272 7419 7830 2325
Size: 1719 2556 398 274 147 411 2662
Fragments arranged by size:
2662 2556 1719 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2462 2492 3197 3227 3374
Size: 30 165 30 1059 30 705 30 147
Cuts at: 3374 3404 1178
Size: 30 5941
Fragments arranged by size:
5941 1059 705 165 147 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2077 2208 3080 3218 3395 4154 1186
Size: 891 131 872 138 177 759 5199
Fragments arranged by size:
5199 891 872 759 177 138 131
BseYI C'CCAG_C
Cuts at: 2152 3554 3881 5397 7370 7866 2152
Size: 1402 327 1516 1973 496 2453
Fragments arranged by size:
2453 1973 1516 1402 496 327
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2181 3259 3394 3488 3515 3530 3776
Size: 573 445 1078 135 94 27 15 246
Cuts at: 3776 4453 4819 1163
Size: 677 366 4511
Fragments arranged by size:
4511 1078 677 573 445 366 246 135
94 27 15
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3733 5617 6378 7406 1540
Size: 275 186 1732 1884 761 1028 2301
Fragments arranged by size:
2301 1884 1732 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2069 3768 4677 6228 6313 7384 2069
Size: 1699 909 1551 85 1071 2852
Fragments arranged by size:
2852 1699 1551 1071 909 85
BsmI GAATG_Cn'
Cuts at: 4262 5678 5771 4262
Size: 1416 93 6658
Fragments arranged by size:
6658 1416 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2069 2145 3478 3768 4108 4677 5354
Size: 399 279 76 1333 290 340 569 677
Cuts at: 5354 5486 6228 6313 7384 1391
Size: 132 742 85 1071 2174
Fragments arranged by size:
2174 1333 1071 742 677 569 399 340
290 279 132 85 76
BspEI T'CCGG_A
Cuts at: 7830 7830
Size: 8167
BspHI T'CATG_A
Cuts at: 3432 3570 4473 4527 4804 6056 7786 3432
Size: 138 903 54 277 1252 1730 3813
Fragments arranged by size:
3813 1730 1252 903 277 138 54
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3452 3454 3672 4671 4705 5522 844
Size: 2608 2 218 999 34 817 3489
Fragments arranged by size:
3489 2608 999 817 218 34 2
BsrBI CCG'CTC
Cuts at: 1394 1823 6054 1394
Size: 429 4231 3507
Fragments arranged by size:
4231 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2041 6662 6836 65
Size: 1183 793 4621 174 1396
Fragments arranged by size:
4621 1396 1183 793 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4428 6811 6998 1700
Size: 110 2618 2383 187 2869
Fragments arranged by size:
2869 2618 2383 187 110
BsrGI T'GTAC_A
Cuts at: 96 3338 96
Size: 3242 4925
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8167
BssSI C'ACGA_G
Cuts at: 3200 3781 6227 7239 3200
Size: 581 2446 1012 4128
Fragments arranged by size:
4128 2446 1012 581
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3595 5224 794
Size: 2801 1629 3737
Fragments arranged by size:
3737 2801 1629
BstBI TT'CG_AA
Cuts at: 2915 6971 2915
Size: 4056 4111
BstEII G'GTnAC_C
Cuts at: 1184 4751 1184
Size: 3567 4600
BstXI CCAn_nnnn'nTGG
Cuts at: 2467 3667 4096 2467
Size: 1200 429 6538
Fragments arranged by size:
6538 1200 429
BstYI r'GATC_y
Cuts at: 1972 3078 3591 3735 3748 4030 4377 4635 4785
Size: 1106 513 144 13 282 347 258 150
Cuts at: 4785 5497 5864 6249 6266 7718 7804 7816 8162
Size: 712 367 385 17 1452 86 12 346
Cuts at: 8162 1972
Size: 1977
Fragments arranged by size:
1977 1452 1106 712 513 385 367 347
346 282 258 150 144 86 17 13
12
BtgI C'CryG_G
Cuts at: 513 1065 2040 4070 4138 4274 5256 5290 513
Size: 552 975 2030 68 136 982 34 3390
Fragments arranged by size:
3390 2030 982 975 552 136 68 34
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4068 487
Size: 683 56 2842 4586
Fragments arranged by size:
4586 2842 683 56
BtsI GCAGTG_nn'
Cuts at: 2164 5683 6477 6497 2164
Size: 3519 794 20 3834
Fragments arranged by size:
3834 3519 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8167
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4578 4594 4613 4629 529
Size: 35 4014 16 19 16 4067
Fragments arranged by size:
4067 4014 35 19 16 16
DraI TTT'AAA
Cuts at: 5463 5471 5463
Size: 8 8159
DraIII CAC_nnn'GTG
Cuts at: 3955 5053 3955
Size: 1098 7069
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7174 809
Size: 507 5858 1802
Fragments arranged by size:
5858 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2589 4098 5293
Size: 54 1425 50 275 18 759 1509 1195
Cuts at: 5293 5614 6503 8132 8
Size: 321 889 1629 43
Fragments arranged by size:
1629 1509 1425 1195 889 759 321 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5614 1537
Size: 275 3802 4090
Fragments arranged by size:
4090 3802 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3081 3199 3837 3999 4040 4545 6097 1353
Size: 1728 118 638 162 41 505 1552 3423
Fragments arranged by size:
3423 1728 1552 638 505 162 118 41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6754 7128 7274 387
Size: 1064 138 5165 374 146 1280
Fragments arranged by size:
5165 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2067 4675 2067
Size: 2608 5559
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2111 2489 2822 3047 3206 3861 4614
Size: 393 479 378 333 225 159 655 753
Cuts at: 4614 4754 5136 6230 6827 7614 7882 1239
Size: 140 382 1094 597 787 268 1524
Fragments arranged by size:
1524 1094 787 753 655 597 479 393
382 378 333 268 225 159 140
EcoNI CCTnn'n_nnAGG
Cuts at: 2103 3743 2103
Size: 1640 6527
EcoO109I rG'GnC_Cy
Cuts at: 2095 3804 4022 2095
Size: 1709 218 6240
Fragments arranged by size:
6240 1709 218
EcoRI G'AATT_C
Cuts at: 5402 5476 5854 5402
Size: 74 378 7715
Fragments arranged by size:
7715 378 74
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4354 4354
Size: 8167
FspI TGC'GCA
Cuts at: 3599 6673 3599
Size: 3074 5093
HaeII r_GCGC'y
Cuts at: 1678 4624 7314 1678
Size: 2946 2690 2531
Fragments arranged by size:
2946 2690 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2110 2142 2291 2323 2447
Size: 32 135 32 538 32 149 32 124
Cuts at: 2447 2479 2947 2979 3197 3229 3715 3747 5183
Size: 32 468 32 218 32 486 32 1436
Cuts at: 5183 5215 6808 6840 6882 6914 1373
Size: 32 1593 32 42 32 2626
Fragments arranged by size:
2626 1593 1436 538 486 468 218 149
135 124 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5511 5757 669
Size: 988 281 3573 246 3079
Fragments arranged by size:
3573 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4544 5425 748
Size: 3796 881 3490
Fragments arranged by size:
3796 3490 881
HpaI GTT'AAC
Cuts at: 5757 5757
Size: 8167
KpnI G_GTAC'C
Cuts at: 5492 5492
Size: 8167
MfeI C'AATT_G
Cuts at: 2347 5085 5766 2347
Size: 2738 681 4748
Fragments arranged by size:
4748 2738 681
MluI A'CGCG_T
Cuts at: 8043 8043
Size: 8167
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2112 3684 5431 7281 7465 1305
Size: 807 1572 1747 1850 184 2007
Fragments arranged by size:
2007 1850 1747 1572 807 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2591 4100 5295 10
Size: 54 1425 343 759 1509 1195 2882
Fragments arranged by size:
2882 1509 1425 1195 759 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2203 2714 2847 2916 3281 3431 4143
Size: 648 1037 511 133 69 365 150 712
Cuts at: 4143 4238 4450 6125 6484 6643 518
Size: 95 212 1675 359 159 2042
Fragments arranged by size:
2042 1675 1037 712 648 511 365 359
212 159 150 133 95 69
MspA1I CmG'CkG
Cuts at: 1606 3721 4096 4482 5583 6260 7408 7653 1606
Size: 2115 375 386 1101 677 1148 245 2120
Fragments arranged by size:
2120 2115 1148 1101 677 386 375 245
NcoI C'CATG_G
Cuts at: 513 1065 2040 4070 4138 4274 5256 5290 513
Size: 552 975 2030 68 136 982 34 3390
Fragments arranged by size:
3390 2030 982 975 552 136 68 34
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8167
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8167
NotI GC'GGCC_GC
Cuts at: 5614 5614
Size: 8167
NsiI A_TGCA'T
Cuts at: 4262 4262
Size: 8167
NspI r_CATG'y
Cuts at: 1132 1229 2208 5525 7070 1132
Size: 97 979 3317 1545 2229
Fragments arranged by size:
3317 2229 1545 979 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8167
PciI A'CATG_T
Cuts at: 7066 7066
Size: 8167
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2265 4071 4353 5291 1108
Size: 1157 1806 282 938 3984
Fragments arranged by size:
3984 1806 1157 938 282
PfoI T'CCnGG_A
Cuts at: 3081 3081
Size: 8167
PmeI GTTT'AAAC
Cuts at: 5471 5471
Size: 8167
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3174 3206 6236 6268 7749 7781 3174
Size: 32 3030 32 1481 32 3560
Fragments arranged by size:
3560 3030 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3804 4022 3804
Size: 218 7949
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8167
PsiI TTA'TAA
Cuts at: 5737 5737
Size: 8167
PstI C_TGCA'G
Cuts at: 830 4682 5519 830
Size: 3852 837 3478
Fragments arranged by size:
3852 3478 837
PvuI CG_AT'CG
Cuts at: 2001 3733 2001
Size: 1732 6435
PvuII CAG'CTG
Cuts at: 3721 4096 3721
Size: 375 7792
RsrII CG'GwC_CG
Cuts at: 3541 3541
Size: 8167
SacI G_AGCT'C
Cuts at: 2069 4677 2069
Size: 2608 5559
SalI G'TCGA_C
Cuts at: 1936 5509 1936
Size: 3573 4594
SanDI GG'GwC_CC
Cuts at: 3804 3804
Size: 8167
SapI GCTCTTCn'nnn_
Cuts at: 4545 4545
Size: 8167
SbfI CC_TGCA'GG
Cuts at: 5519 5519
Size: 8167
ScaI AGT'ACT
Cuts at: 6415 8127 6415
Size: 1712 6455
SexAI A'CCwGG_T
Cuts at: 4131 4131
Size: 8167
SfcI C'TryA_G
Cuts at: 826 1045 3523 4678 5515 6650 7331 7522 826
Size: 219 2478 1155 837 1135 681 191 1471
Fragments arranged by size:
2478 1471 1155 1135 837 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8167
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8167
SmaI CCC'GGG
Cuts at: 1872 5494 1872
Size: 3622 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3581 3740 4789 4895 6271 7172
Size: 197 926 1638 159 1049 106 1376 901
Cuts at: 7172 7434 820
Size: 262 1553
Fragments arranged by size:
1638 1553 1376 1049 926 901 262 197
159 106
SnaBI TAC'GTA
Cuts at: 493 5958 493
Size: 5465 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8167
SphI G_CATG'C
Cuts at: 1132 2208 5525 1132
Size: 1076 3317 3774
Fragments arranged by size:
3774 3317 1076
SspI AAT'ATT
Cuts at: 5 52 2859 6091 5
Size: 47 2807 3232 2081
Fragments arranged by size:
3232 2807 2081 47
StuI AGG'CCT
Cuts at: 1517 3209 4510 1517
Size: 1692 1301 5174
Fragments arranged by size:
5174 1692 1301
StyI C'CwwG_G
Cuts at: 513 1065 2040 2640 2823 3769 4070 4138 4274
Size: 552 975 600 183 946 301 68 136
Cuts at: 4274 5256 5290 5606 513
Size: 982 34 316 3074
Fragments arranged by size:
3074 982 975 946 600 552 316 301
183 136 68 34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2552 6194 6211 6364 6549 6994 325
Size: 1255 972 3642 17 153 185 445 1498
Fragments arranged by size:
3642 1498 1255 972 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2166 3338 4151
Size: 274 80 33 51 1443 189 1172 813
Cuts at: 4151 4821 6413 8125 96
Size: 670 1592 1712 138
Fragments arranged by size:
1712 1592 1443 1172 813 670 274 189
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 8127 8159 8127
Size: 32 8135
Tth111I GACn'n_nGTC
Cuts at: 1316 4133 1316
Size: 2817 5350
XbaI T'CTAG_A
Cuts at: 5503 5503
Size: 8167
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3667 5057 3667
Size: 1390 6777
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8167
XmaI C'CCGG_G
Cuts at: 1870 5492 1870
Size: 3622 4545
XmnI GAAnn'nnTTC
Cuts at: 3485 4905 6296 3485
Size: 1420 1391 5356
Fragments arranged by size:
5356 1420 1391
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5194 5975 276
Size: 53 83 186 936 3660 781 2468
Fragments arranged by size:
3660 2468 936 781 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BbeI BplI BsiWI BsmBI
BstZ17I Bsu36I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PmlI PspOMI PspXI PsrI SacII
SfiI SfoI SrfI SwaI