Restriction map for
pFN21AB3614
(Circular) (Six-base) MAPSORT of: pFN21AB3614.seq Check: 5404 from: 1 to: 5959
pFN21AB3614 5959 bp
With 182 enzymes: SgfI *
October 20, 2009 10:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3769 278
Size: 53 83 186 936 2233 2468
Fragments arranged by size:
2468 2233 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3302 1937
Size: 1365 4594
Acc65I G'GTAC_C
Cuts at: 3280 3280
Size: 5959
AclI AA'CG_TT
Cuts at: 1563 4086 4459 1563
Size: 2523 373 3063
Fragments arranged by size:
3063 2523 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4022 5406 5674 1632
Size: 2390 1384 268 1917
Fragments arranged by size:
2390 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5762
AflIII A'CryG_T
Cuts at: 1203 3183 4858 5835 1203
Size: 1980 1675 977 1327
Fragments arranged by size:
1980 1675 1327 977
AgeI A'CCGG_T
Cuts at: 4790 4790
Size: 5959
AhdI GACnn_n'nnGTC
Cuts at: 4688 4688
Size: 5959
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5959
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5959
AlwNI CAG_nnn'CTG
Cuts at: 2303 3702 5274 2303
Size: 1399 1572 2988
Fragments arranged by size:
2988 1572 1399
ApaLI G'TGCA_C
Cuts at: 4016 5172 4016
Size: 1156 4803
ApoI r'AATT_y
Cuts at: 1493 2607 2770 3079 3268 3494 3646 5755 1493
Size: 1114 163 309 189 226 152 2109 1697
Fragments arranged by size:
2109 1697 1114 309 226 189 163 152
AseI AT'TA_AT
Cuts at: 160 1032 4513 160
Size: 872 3481 1606
Fragments arranged by size:
3481 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5959
AvaI C'yCGr_G
Cuts at: 1870 1943 3284 1870
Size: 73 1341 4545
Fragments arranged by size:
4545 1341 73
AvrII C'CTAG_G
Cuts at: 2678 2678
Size: 5959
BamHI G'GATC_C
Cuts at: 3289 3656 3289
Size: 367 5592
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3280 4735 618
Size: 325 217 627 1493 1455 1842
Fragments arranged by size:
1842 1493 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3097 3278 1391
Size: 1706 181 4072
Fragments arranged by size:
4072 1706 181
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4988
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4150 4184 1903
Size: 34 2213 34 3678
Fragments arranged by size:
3678 2213 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3851 5061 3851
Size: 1210 4749
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5959
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4570 136
Size: 107 122 71 631 605 2898 1525
Fragments arranged by size:
2898 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5954 5954
Size: 5959
BlpI GC'TnA_GC
Cuts at: 3376 3376
Size: 5959
Bme1580I G_kGCm'C
Cuts at: 1790 4020 5176 1790
Size: 2230 1156 2573
Fragments arranged by size:
2573 2230 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5959
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3690 4648 442
Size: 416 53 83 399 24 2273 958 1753
Fragments arranged by size:
2273 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5959
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2745 3111 4619 1239
Size: 1506 366 1508 2579
Fragments arranged by size:
2579 1508 1506 366
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2094 4084 4949 5247 2094
Size: 1990 865 298 2806
Fragments arranged by size:
2806 1990 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4622 882
Size: 617 3123 2219
Fragments arranged by size:
3123 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3750 493
Size: 711 2546 2702
Fragments arranged by size:
2702 2546 711
BsaBI GATnn'nnATC
Cuts at: 5953 5953
Size: 5959
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2506 3766 4148 4783
Size: 53 83 186 936 973 1260 382 635
Cuts at: 4783 275
Size: 1451
Fragments arranged by size:
1451 1260 973 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2036 2715 4392 4790 5064 5211 5622 2036
Size: 679 1677 398 274 147 411 2373
Fragments arranged by size:
2373 1677 679 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3039 3069 1178
Size: 30 165 30 1636 30 4068
Fragments arranged by size:
4068 1636 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5959
BseYI C'CCAG_C
Cuts at: 5162 5658 5162
Size: 496 5463
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5386
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3409 4170 5198 1540
Size: 275 186 1408 761 1028 2301
Fragments arranged by size:
2301 1408 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4020 4105 5176 4020
Size: 85 1071 4803
Fragments arranged by size:
4803 1071 85
BsmI GAATG_Cn'
Cuts at: 2652 3470 3563 2652
Size: 818 93 5048
Fragments arranged by size:
5048 818 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3097 3278 4020 4105 5176 1391
Size: 399 1307 181 742 85 1071 2174
Fragments arranged by size:
2174 1307 1071 742 399 181 85
BspEI T'CCGG_A
Cuts at: 5622 5622
Size: 5959
BspHI T'CATG_A
Cuts at: 2670 3848 5578 2670
Size: 1178 1730 3051
Fragments arranged by size:
3051 1730 1178
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3314 844
Size: 2470 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3846 1394
Size: 429 2023 3507
Fragments arranged by size:
3507 2023 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4454 4628 65
Size: 1183 3206 174 1396
Fragments arranged by size:
3206 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4603 4790 1700
Size: 110 2793 187 2869
Fragments arranged by size:
2869 2793 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5959
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5959
BssSI C'ACGA_G
Cuts at: 4019 5031 4019
Size: 1012 4947
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5959
BstBI TT'CG_AA
Cuts at: 4763 4763
Size: 5959
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5959
BstYI r'GATC_y
Cuts at: 1972 2588 3138 3289 3656 4041 4058 5510 5596
Size: 616 550 151 367 385 17 1452 86
Cuts at: 5596 5608 5954 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 616 550 385 367 346 151
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2501 513
Size: 552 1436 3971
Fragments arranged by size:
3971 1436 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5220
Fragments arranged by size:
5220 683 56
BtsI GCAGTG_nn'
Cuts at: 3475 4269 4289 3475
Size: 794 20 5145
Fragments arranged by size:
5145 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5959
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5924
DraI TTT'AAA
Cuts at: 3211 3263 3211
Size: 52 5907
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4966 809
Size: 507 3650 1802
Fragments arranged by size:
3650 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3406 4295 5924
Size: 54 1425 50 275 18 1576 889 1629
Cuts at: 5924 8
Size: 43
Fragments arranged by size:
1629 1576 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3406 1537
Size: 275 1594 4090
Fragments arranged by size:
4090 1594 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2060 2201 3102 3889 1353
Size: 707 141 901 787 3423
Fragments arranged by size:
3423 901 787 707 141
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4546 4920 5066 387
Size: 1064 138 2957 374 146 1280
Fragments arranged by size:
2957 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2745 3111 4022 4619 5406 5674 1239
Size: 393 1113 366 911 597 787 268 1524
Fragments arranged by size:
1524 1113 911 787 597 393 366 268
EcoRI G'AATT_C
Cuts at: 3268 3646 3268
Size: 378 5581
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2215 2215
Size: 5959
FspI TGC'GCA
Cuts at: 4465 4465
Size: 5959
HaeII r_GCGC'y
Cuts at: 1678 5106 1678
Size: 3428 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 1999 2031 2580 2612 4600
Size: 32 135 32 427 32 549 32 1988
Cuts at: 4600 4632 4674 4706 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1988 549 427 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2437 3303 3549 669
Size: 988 281 499 866 246 3079
Fragments arranged by size:
3079 988 866 499 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5959
HpaI GTT'AAC
Cuts at: 3549 3549
Size: 5959
KpnI G_GTAC'C
Cuts at: 3284 3284
Size: 5959
MfeI C'AATT_G
Cuts at: 2886 3558 2886
Size: 672 5287
MluI A'CGCG_T
Cuts at: 5835 5835
Size: 5959
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2517 2800 5073 5257 1305
Size: 1212 283 2273 184 2007
Fragments arranged by size:
2273 2007 1212 283 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4137
Fragments arranged by size:
4137 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3917 4276 4435 518
Size: 648 2751 359 159 2042
Fragments arranged by size:
2751 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2488 3375 4052 5200 5445 1606
Size: 882 887 677 1148 245 2120
Fragments arranged by size:
2120 1148 887 882 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2501 513
Size: 552 1436 3971
Fragments arranged by size:
3971 1436 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5959
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5959
NotI GC'GGCC_GC
Cuts at: 3406 3406
Size: 5959
NspI r_CATG'y
Cuts at: 1132 1229 2947 3187 3317 4862 1132
Size: 97 1718 240 130 1545 2229
Fragments arranged by size:
2229 1718 1545 240 130 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5959
PciI A'CATG_T
Cuts at: 3183 4858 3183
Size: 1675 4284
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5959
PmeI GTTT'AAAC
Cuts at: 3263 3263
Size: 5959
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4028 4060 5541 5573 4028
Size: 32 1481 32 4414
Fragments arranged by size:
4414 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5959
PsiI TTA'TAA
Cuts at: 3529 3529
Size: 5959
PstI C_TGCA'G
Cuts at: 830 3311 830
Size: 2481 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5959
PvuII CAG'CTG
Cuts at: 2488 2488
Size: 5959
SalI G'TCGA_C
Cuts at: 1936 3301 1936
Size: 1365 4594
SapI GCTCTTCn'nnn_
Cuts at: 2060 2201 2060
Size: 141 5818
SbfI CC_TGCA'GG
Cuts at: 3311 3311
Size: 5959
ScaI AGT'ACT
Cuts at: 4207 5919 4207
Size: 1712 4247
SfcI C'TryA_G
Cuts at: 826 1045 2592 3307 4442 5123 5314 826
Size: 219 1547 715 1135 681 191 1471
Fragments arranged by size:
1547 1471 1135 715 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5959
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5959
SmaI CCC'GGG
Cuts at: 1872 3286 1872
Size: 1414 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2109 4063 4964 5226 820
Size: 197 926 166 1954 901 262 1553
Fragments arranged by size:
1954 1553 926 901 262 197 166
SnaBI TAC'GTA
Cuts at: 493 3750 493
Size: 3257 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5959
SphI G_CATG'C
Cuts at: 1132 3317 1132
Size: 2185 3774
SspI AAT'ATT
Cuts at: 5 52 3883 5
Size: 47 3831 2081
Fragments arranged by size:
3831 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5959
StyI C'CwwG_G
Cuts at: 513 1065 2501 2678 3012 3398 513
Size: 552 1436 177 334 386 3074
Fragments arranged by size:
3074 1436 552 386 334 177
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2234 2992 3986 4003 4156 4341 4786
Size: 1255 654 758 994 17 153 185 445
Cuts at: 4786 325
Size: 1498
Fragments arranged by size:
1498 1255 994 758 654 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2523 4205 5917
Size: 274 80 33 51 1443 546 1682 1712
Cuts at: 5917 96
Size: 138
Fragments arranged by size:
1712 1682 1443 546 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2916 2948 3039 3071 5919 5951 2916
Size: 32 91 32 2848 32 2924
Fragments arranged by size:
2924 2848 91 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5959
XbaI T'CTAG_A
Cuts at: 2050 3295 2050
Size: 1245 4714
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5959
XmaI C'CCGG_G
Cuts at: 1870 3284 1870
Size: 1414 4545
XmnI GAAnn'nnTTC
Cuts at: 4088 4088
Size: 5959
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3767 276
Size: 53 83 186 936 2233 2468
Fragments arranged by size:
2468 2233 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SexAI SfiI SfoI SrfI SwaI XcmI