Restriction map for
pFN21AB3608
(Circular) (Six-base) MAPSORT of: pFN21AB3608.seq Check: 5383 from: 1 to: 5335
pFN21AB3608 5335 bp
With 182 enzymes: SgfI *
August 16, 2010 14:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3145 278
Size: 53 83 186 936 1609 2468
Fragments arranged by size:
2468 1609 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2678 1937
Size: 741 4594
Acc65I G'GTAC_C
Cuts at: 2656 2656
Size: 5335
AclI AA'CG_TT
Cuts at: 1563 3462 3835 1563
Size: 1899 373 3063
Fragments arranged by size:
3063 1899 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2636 3398 4782 5050 1632
Size: 1004 762 1384 268 1917
Fragments arranged by size:
1917 1384 1004 762 268
AfeI AGC'GCT
Cuts at: 2297 2297
Size: 5335
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5138
AflIII A'CryG_T
Cuts at: 1203 4234 5211 1203
Size: 3031 977 1327
Fragments arranged by size:
3031 1327 977
AgeI A'CCGG_T
Cuts at: 4166 4166
Size: 5335
AhdI GACnn_n'nnGTC
Cuts at: 4064 4064
Size: 5335
AleI CACnn'nnGTG
Cuts at: 1166 2216 2345 1166
Size: 1050 129 4156
Fragments arranged by size:
4156 1050 129
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5335
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2146 2178 2146
Size: 32 5303
AlwNI CAG_nnn'CTG
Cuts at: 3078 4650 3078
Size: 1572 3763
ApaLI G'TGCA_C
Cuts at: 3392 4548 3392
Size: 1156 4179
ApoI r'AATT_y
Cuts at: 1493 2585 2644 2870 3022 5131 1493
Size: 1092 59 226 152 2109 1697
Fragments arranged by size:
2109 1697 1092 226 152 59
AseI AT'TA_AT
Cuts at: 160 1032 3889 160
Size: 872 2857 1606
Fragments arranged by size:
2857 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5335
AvaI C'yCGr_G
Cuts at: 1870 1943 2488 2529 2660 1870
Size: 73 545 41 131 4545
Fragments arranged by size:
4545 545 131 73 41
BamHI G'GATC_C
Cuts at: 2665 3032 2665
Size: 367 4968
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2235 2656 4111 618
Size: 325 217 627 448 421 1455 1842
Fragments arranged by size:
1842 1455 627 448 421 325 217
BanII G_rGCy'C
Cuts at: 1391 2654 1391
Size: 1263 4072
BbeI G_GCGC'C
Cuts at: 2239 2239
Size: 5335
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2436 928
Size: 971 537 3827
Fragments arranged by size:
3827 971 537
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3526 3560 1903
Size: 34 50 34 1505 34 3678
Fragments arranged by size:
3678 1505 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3227 4437 3227
Size: 1210 4125
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5335
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3946 136
Size: 107 122 71 631 605 2274 1525
Fragments arranged by size:
2274 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2226 5330 2226
Size: 3104 2231
BlpI GC'TnA_GC
Cuts at: 2178 2752 2178
Size: 574 4761
Bme1580I G_kGCm'C
Cuts at: 1790 3396 4552 1790
Size: 1606 1156 2573
Fragments arranged by size:
2573 1606 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5335
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3066 4024 442
Size: 416 53 83 399 24 1649 958 1753
Fragments arranged by size:
1753 1649 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5335
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2157 2157
Size: 5335
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2139 2150 3995 1239
Size: 900 11 1845 2579
Fragments arranged by size:
2579 1845 900 11
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3460 4325 4623 3460
Size: 865 298 4172
Fragments arranged by size:
4172 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2055 2615 3998 882
Size: 617 556 560 1383 2219
Fragments arranged by size:
2219 1383 617 560 556
BsaAI yAC'GTr
Cuts at: 493 1204 3126 493
Size: 711 1922 2702
Fragments arranged by size:
2702 1922 711
BsaBI GATnn'nnATC
Cuts at: 5329 5329
Size: 5335
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2236 3142 3524 4159
Size: 53 83 186 936 703 906 382 635
Cuts at: 4159 275
Size: 1451
Fragments arranged by size:
1451 936 906 703 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2580 3768 4166 4440 4587 4998 2580
Size: 1188 398 274 147 411 2917
Fragments arranged by size:
2917 1188 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2146 2176 1178
Size: 30 165 30 743 30 4337
Fragments arranged by size:
4337 743 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5335
BseYI C'CCAG_C
Cuts at: 4538 5034 4538
Size: 496 4839
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2039 2312 2348 2369 2451 1163
Size: 573 303 273 36 21 82 4047
Fragments arranged by size:
4047 573 303 273 82 36 21
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2785 3546 4574 1540
Size: 275 186 784 761 1028 2301
Fragments arranged by size:
2301 1028 784 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3396 3481 4552 3396
Size: 85 1071 4179
Fragments arranged by size:
4179 1071 85
BsmI GAATG_Cn'
Cuts at: 2846 2939 2846
Size: 93 5242
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2654 3396 3481 4552 1391
Size: 399 864 742 85 1071 2174
Fragments arranged by size:
2174 1071 864 742 399 85
BspEI T'CCGG_A
Cuts at: 4998 4998
Size: 5335
BspHI T'CATG_A
Cuts at: 3224 4954 3224
Size: 1730 3605
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2690 844
Size: 1846 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2028 2109 3222 1394
Size: 429 205 81 1113 3507
Fragments arranged by size:
3507 1113 429 205 81
BsrDI GCAATG_nn'
Cuts at: 65 1248 2172 3830 4004 65
Size: 1183 924 1658 174 1396
Fragments arranged by size:
1658 1396 1183 924 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2182 2559 3979 4166 1700
Size: 110 372 377 1420 187 2869
Fragments arranged by size:
2869 1420 377 372 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5335
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5335
BssSI C'ACGA_G
Cuts at: 2142 3395 4407 2142
Size: 1253 1012 3070
Fragments arranged by size:
3070 1253 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5335
BstBI TT'CG_AA
Cuts at: 4139 4139
Size: 5335
BstEII G'GTnAC_C
Cuts at: 1184 2533 1184
Size: 1349 3986
BstXI CCAn_nnnn'nTGG
Cuts at: 2266 2266
Size: 5335
BstYI r'GATC_y
Cuts at: 1972 2226 2665 3032 3417 3434 4886 4972 4984
Size: 254 439 367 385 17 1452 86 12
Cuts at: 4984 5330 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 439 385 367 346 254 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2415 513
Size: 552 1350 3433
Fragments arranged by size:
3433 1350 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4596
Fragments arranged by size:
4596 683 56
BtsI GCAGTG_nn'
Cuts at: 2336 2851 3645 3665 2336
Size: 515 794 20 4006
Fragments arranged by size:
4006 794 515 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5335
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5300
DraI TTT'AAA
Cuts at: 2639 2639
Size: 5335
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4342 809
Size: 507 3026 1802
Fragments arranged by size:
3026 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2184 2273 2782
Size: 54 1425 50 275 18 354 89 509
Cuts at: 2782 3671 5300 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 509 354 275 89 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2782 1537
Size: 275 970 4090
Fragments arranged by size:
4090 970 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3265 1353
Size: 1912 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2546 3922 4296 4442 387
Size: 1064 138 957 1376 374 146 1280
Fragments arranged by size:
1376 1280 1064 957 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2139 2150 2636 3398 3995 4782 5050
Size: 393 507 11 486 762 597 787 268
Cuts at: 5050 1239
Size: 1524
Fragments arranged by size:
1524 787 762 597 507 486 393 268
11
EcoRI G'AATT_C
Cuts at: 2644 3022 2644
Size: 378 4957
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2419 2419
Size: 5335
FspI TGC'GCA
Cuts at: 3841 3841
Size: 5335
HaeII r_GCGC'y
Cuts at: 1678 2239 2299 4482 1678
Size: 561 60 2183 2531
Fragments arranged by size:
2531 2183 561 60
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3976 4008 4050 4082 1373
Size: 32 135 32 2404 32 42 32 2626
Fragments arranged by size:
2626 2404 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2679 2925 669
Size: 988 281 741 246 3079
Fragments arranged by size:
3079 988 741 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5335
HpaI GTT'AAC
Cuts at: 2925 2925
Size: 5335
KasI G'GCGC_C
Cuts at: 2235 2235
Size: 5335
KpnI G_GTAC'C
Cuts at: 2660 2660
Size: 5335
MfeI C'AATT_G
Cuts at: 2934 2934
Size: 5335
MluI A'CGCG_T
Cuts at: 5211 5211
Size: 5335
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2068 2070 4449 4633 1305
Size: 763 2 2379 184 2007
Fragments arranged by size:
2379 2007 763 184 2
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3513
Fragments arranged by size:
3513 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2216 2345 2446 2598 3293 3652 3811
Size: 648 1050 129 101 152 695 359 159
Cuts at: 3811 518
Size: 2042
Fragments arranged by size:
2042 1050 695 648 359 159 152 129
101
MspA1I CmG'CkG
Cuts at: 1606 2353 2465 2751 3428 4576 4821 1606
Size: 747 112 286 677 1148 245 2120
Fragments arranged by size:
2120 1148 747 677 286 245 112
NaeI GCC'GGC
Cuts at: 2184 2561 2184
Size: 377 4958
NarI GG'CG_CC
Cuts at: 2236 2236
Size: 5335
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4783
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5335
NgoMIV G'CCGG_C
Cuts at: 2182 2559 2182
Size: 377 4958
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5335
NotI GC'GGCC_GC
Cuts at: 2782 2782
Size: 5335
NspI r_CATG'y
Cuts at: 1132 1229 2445 2693 4238 1132
Size: 97 1216 248 1545 2229
Fragments arranged by size:
2229 1545 1216 248 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5335
PciI A'CATG_T
Cuts at: 4234 4234
Size: 5335
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5335
PmeI GTTT'AAAC
Cuts at: 2639 2639
Size: 5335
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2146 2178 3404 3436 4917 4949 2146
Size: 32 1226 32 1481 32 2532
Fragments arranged by size:
2532 1481 1226 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5335
PsiI TTA'TAA
Cuts at: 2905 2905
Size: 5335
PstI C_TGCA'G
Cuts at: 830 2687 830
Size: 1857 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5335
PvuII CAG'CTG
Cuts at: 2353 2465 2353
Size: 112 5223
SalI G'TCGA_C
Cuts at: 1936 2677 1936
Size: 741 4594
SbfI CC_TGCA'GG
Cuts at: 2687 2687
Size: 5335
ScaI AGT'ACT
Cuts at: 3583 5295 3583
Size: 1712 3623
SfcI C'TryA_G
Cuts at: 826 1045 2683 3818 4499 4690 826
Size: 219 1638 1135 681 191 1471
Fragments arranged by size:
1638 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2237 2237
Size: 5335
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5335
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5335
SmaI CCC'GGG
Cuts at: 1872 2490 2662 1872
Size: 618 172 4545
Fragments arranged by size:
4545 618 172
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3439 4340 4602 820
Size: 197 926 1496 901 262 1553
Fragments arranged by size:
1553 1496 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3126 493
Size: 2633 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5335
SphI G_CATG'C
Cuts at: 1132 2445 2693 1132
Size: 1313 248 3774
Fragments arranged by size:
3774 1313 248
SspI AAT'ATT
Cuts at: 5 52 3259 5
Size: 47 3207 2081
Fragments arranged by size:
3207 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5335
StyI C'CwwG_G
Cuts at: 513 1065 2568 2626 2774 513
Size: 552 1503 58 148 3074
Fragments arranged by size:
3074 1503 552 148 58
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2357 3362 3379 3532 3717 4162 325
Size: 1255 777 1005 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1005 777 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3581 5293 96
Size: 274 80 33 51 1443 1604 1712 138
Fragments arranged by size:
1712 1604 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5295 5327 5295
Size: 32 5303
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5335
XbaI T'CTAG_A
Cuts at: 2671 2671
Size: 5335
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2422 2422
Size: 5335
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5335
XmaI C'CCGG_G
Cuts at: 1870 2488 2660 1870
Size: 618 172 4545
Fragments arranged by size:
4545 618 172
XmnI GAAnn'nnTTC
Cuts at: 3464 3464
Size: 5335
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3143 276
Size: 53 83 186 936 1609 2468
Fragments arranged by size:
2468 1609 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I
EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SrfI SwaI