Restriction map for
pFN21AB3606
(Circular) (Six-base) MAPSORT of: pFN21AB3606.seq Check: 9008 from: 1 to: 5299
pFN21AB3606 5299 bp
With 182 enzymes: SgfI *
October 20, 2009 10:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3109 278
Size: 53 83 186 936 1573 2468
Fragments arranged by size:
2468 1573 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2642 1937
Size: 705 4594
Acc65I G'GTAC_C
Cuts at: 2620 2620
Size: 5299
AclI AA'CG_TT
Cuts at: 1563 3426 3799 1563
Size: 1863 373 3063
Fragments arranged by size:
3063 1863 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3362 4746 5014 1632
Size: 1730 1384 268 1917
Fragments arranged by size:
1917 1730 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5102
AflIII A'CryG_T
Cuts at: 1203 4198 5175 1203
Size: 2995 977 1327
Fragments arranged by size:
2995 1327 977
AgeI A'CCGG_T
Cuts at: 4130 4130
Size: 5299
AhdI GACnn_n'nnGTC
Cuts at: 4028 4028
Size: 5299
AleI CACnn'nnGTG
Cuts at: 1166 2060 2150 1166
Size: 894 90 4315
Fragments arranged by size:
4315 894 90
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5299
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2228 2260 2228
Size: 32 5267
AlwNI CAG_nnn'CTG
Cuts at: 2042 3042 4614 2042
Size: 1000 1572 2727
Fragments arranged by size:
2727 1572 1000
ApaI G_GGCC'C
Cuts at: 2297 2297
Size: 5299
ApaLI G'TGCA_C
Cuts at: 3356 4512 3356
Size: 1156 4143
ApoI r'AATT_y
Cuts at: 1493 2608 2834 2986 5095 1493
Size: 1115 226 152 2109 1697
Fragments arranged by size:
2109 1697 1115 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3853 160
Size: 872 2821 1606
Fragments arranged by size:
2821 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5299
AvaI C'yCGr_G
Cuts at: 1870 1943 2624 1870
Size: 73 681 4545
Fragments arranged by size:
4545 681 73
BamHI G'GATC_C
Cuts at: 2629 2996 2629
Size: 367 4932
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2072 2620 4075 618
Size: 325 217 627 285 548 1455 1842
Fragments arranged by size:
1842 1455 627 548 325 285 217
BanII G_rGCy'C
Cuts at: 1391 2297 2618 1391
Size: 906 321 4072
Fragments arranged by size:
4072 906 321
BbeI G_GCGC'C
Cuts at: 2076 2076
Size: 5299
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4328
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3490 3524 1903
Size: 34 1553 34 3678
Fragments arranged by size:
3678 1553 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3191 4401 3191
Size: 1210 4089
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5299
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3910 136
Size: 107 122 71 631 605 2238 1525
Fragments arranged by size:
2238 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5294 5294
Size: 5299
BlpI GC'TnA_GC
Cuts at: 2716 2716
Size: 5299
Bme1580I G_kGCm'C
Cuts at: 1790 2159 2297 3360 4516 1790
Size: 369 138 1063 1156 2573
Fragments arranged by size:
2573 1156 1063 369 138
BmgBI CAC'GTC
Cuts at: 1318 2323 1318
Size: 1005 4294
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3030 3988 442
Size: 416 53 83 399 24 1613 958 1753
Fragments arranged by size:
1753 1613 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5299
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3959 1239
Size: 2720 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3424 4289 4587 3424
Size: 865 298 4136
Fragments arranged by size:
4136 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3962 882
Size: 617 2463 2219
Fragments arranged by size:
2463 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2116 3090 493
Size: 711 912 974 2702
Fragments arranged by size:
2702 974 912 711
BsaBI GATnn'nnATC
Cuts at: 5293 5293
Size: 5299
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2073 2226 3106 3488
Size: 53 83 186 936 540 153 880 382
Cuts at: 3488 4123 275
Size: 635 1451
Fragments arranged by size:
1451 936 880 635 540 382 186 153
83 53
BsaWI w'CCGG_w
Cuts at: 2012 3732 4130 4404 4551 4962 2012
Size: 1720 398 274 147 411 2349
Fragments arranged by size:
2349 1720 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5074
Fragments arranged by size:
5074 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5299
BseYI C'CCAG_C
Cuts at: 2206 2221 4502 4998 2206
Size: 15 2281 496 2507
Fragments arranged by size:
2507 2281 496 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2208 1163
Size: 573 472 4254
Fragments arranged by size:
4254 573 472
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2749 3510 4538 1540
Size: 275 186 748 761 1028 2301
Fragments arranged by size:
2301 1028 761 748 275 186
BsiHKAI G_wGCw'C
Cuts at: 3360 3445 4516 3360
Size: 85 1071 4143
Fragments arranged by size:
4143 1071 85
BsmI GAATG_Cn'
Cuts at: 2810 2903 2810
Size: 93 5206
BsmBI CGTCTCn'nnnn_
Cuts at: 2233 2233
Size: 5299
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2159 2297 2618 3360 3445 4516 1391
Size: 399 369 138 321 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 369 321 138 85
BspEI T'CCGG_A
Cuts at: 4962 4962
Size: 5299
BspHI T'CATG_A
Cuts at: 3188 4918 3188
Size: 1730 3569
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2654 844
Size: 1810 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3186 1394
Size: 429 1363 3507
Fragments arranged by size:
3507 1363 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2373 3794 3968 65
Size: 1183 1125 1421 174 1396
Fragments arranged by size:
1421 1396 1183 1125 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3943 4130 1700
Size: 110 2133 187 2869
Fragments arranged by size:
2869 2133 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5299
BssHII G'CGCG_C
Cuts at: 1925 2021 1925
Size: 96 5203
BssSI C'ACGA_G
Cuts at: 3359 4371 3359
Size: 1012 4287
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5299
BstBI TT'CG_AA
Cuts at: 4103 4103
Size: 5299
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5299
BstXI CCAn_nnnn'nTGG
Cuts at: 2062 2062
Size: 5299
BstYI r'GATC_y
Cuts at: 1972 2629 2996 3381 3398 4850 4936 4948 5294
Size: 657 367 385 17 1452 86 12 346
Cuts at: 5294 1972
Size: 1977
Fragments arranged by size:
1977 1452 657 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2457 513
Size: 552 939 453 3355
Fragments arranged by size:
3355 939 552 453
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2155 487
Size: 683 56 929 3631
Fragments arranged by size:
3631 929 683 56
BtsI GCAGTG_nn'
Cuts at: 2548 2815 3609 3629 2548
Size: 267 794 20 4218
Fragments arranged by size:
4218 794 267 20
ClaI AT'CG_AT
Cuts at: 1554 2400 1554
Size: 846 4453
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2240 2275 529
Size: 35 1676 35 3553
Fragments arranged by size:
3553 1676 35 35
DraI TTT'AAA
Cuts at: 2603 2603
Size: 5299
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4306 809
Size: 507 2990 1802
Fragments arranged by size:
2990 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2746 3635 5264
Size: 54 1425 50 275 18 916 889 1629
Cuts at: 5264 8
Size: 43
Fragments arranged by size:
1629 1425 916 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2746 1537
Size: 275 934 4090
Fragments arranged by size:
4090 934 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3229 1353
Size: 1876 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3886 4260 4406 387
Size: 1064 138 2297 374 146 1280
Fragments arranged by size:
2297 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3362 3959 4746 5014 1239
Size: 393 1730 597 787 268 1524
Fragments arranged by size:
1730 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2294 2386 2294
Size: 92 5207
EcoRI G'AATT_C
Cuts at: 2608 2986 2608
Size: 378 4921
FspI TGC'GCA
Cuts at: 3805 3805
Size: 5299
HaeII r_GCGC'y
Cuts at: 1678 2076 4446 1678
Size: 398 2370 2531
Fragments arranged by size:
2531 2370 398
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3940 3972 4014 4046 1373
Size: 32 135 32 2368 32 42 32 2626
Fragments arranged by size:
2626 2368 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2643 2889 669
Size: 988 281 705 246 3079
Fragments arranged by size:
3079 988 705 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5299
HpaI GTT'AAC
Cuts at: 2889 2889
Size: 5299
KasI G'GCGC_C
Cuts at: 2072 2072
Size: 5299
KpnI G_GTAC'C
Cuts at: 2624 2624
Size: 5299
MfeI C'AATT_G
Cuts at: 2898 2898
Size: 5299
MluI A'CGCG_T
Cuts at: 5175 5175
Size: 5299
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2076 4413 4597 1305
Size: 771 2337 184 2007
Fragments arranged by size:
2337 2007 771 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3477
Fragments arranged by size:
3477 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2060 2150 3257 3616 3775 518
Size: 648 894 90 1107 359 159 2042
Fragments arranged by size:
2042 1107 894 648 359 159 90
MspA1I CmG'CkG
Cuts at: 1606 2060 2525 2715 3392 4540 4785 1606
Size: 454 465 190 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 465 454 245 190
NarI GG'CG_CC
Cuts at: 2073 2073
Size: 5299
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3808
Fragments arranged by size:
3808 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5299
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5299
NotI GC'GGCC_GC
Cuts at: 2746 2746
Size: 5299
NspI r_CATG'y
Cuts at: 1132 1229 2657 4202 1132
Size: 97 1428 1545 2229
Fragments arranged by size:
2229 1545 1428 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5299
PciI A'CATG_T
Cuts at: 4198 4198
Size: 5299
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5299
PmeI GTTT'AAAC
Cuts at: 2603 2603
Size: 5299
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3368 3400 4881 4913 3368
Size: 32 1481 32 3754
Fragments arranged by size:
3754 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2335 1613
Size: 722 4577
PsiI TTA'TAA
Cuts at: 2869 2869
Size: 5299
PspOMI G'GGCC_C
Cuts at: 2293 2293
Size: 5299
PstI C_TGCA'G
Cuts at: 830 2651 830
Size: 1821 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5299
SalI G'TCGA_C
Cuts at: 1936 2641 1936
Size: 705 4594
SbfI CC_TGCA'GG
Cuts at: 2651 2651
Size: 5299
ScaI AGT'ACT
Cuts at: 3547 5259 3547
Size: 1712 3587
SfcI C'TryA_G
Cuts at: 826 1045 2380 2647 3782 4463 4654 826
Size: 219 1335 267 1135 681 191 1471
Fragments arranged by size:
1471 1335 1135 681 267 219 191
SfoI GGC'GCC
Cuts at: 2074 2074
Size: 5299
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5299
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5299
SmaI CCC'GGG
Cuts at: 1872 2626 1872
Size: 754 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3403 4304 4566 820
Size: 197 926 1460 901 262 1553
Fragments arranged by size:
1553 1460 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3090 493
Size: 2597 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5299
SphI G_CATG'C
Cuts at: 1132 2657 1132
Size: 1525 3774
SspI AAT'ATT
Cuts at: 5 52 2582 3223 5
Size: 47 2530 641 2081
Fragments arranged by size:
2530 2081 641 47
StuI AGG'CCT
Cuts at: 1517 2486 1517
Size: 969 4330
StyI C'CwwG_G
Cuts at: 513 1065 2004 2738 513
Size: 552 939 734 3074
Fragments arranged by size:
3074 939 734 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2264 3326 3343 3496 3681 4126 325
Size: 1255 684 1062 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1062 684 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2493 3545 5257
Size: 274 80 33 51 1443 516 1052 1712
Cuts at: 5257 96
Size: 138
Fragments arranged by size:
1712 1443 1052 516 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2442 2474 5259 5291 2442
Size: 32 2785 32 2450
Fragments arranged by size:
2785 2450 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5299
XbaI T'CTAG_A
Cuts at: 2635 2635
Size: 5299
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5299
XmaI C'CCGG_G
Cuts at: 1870 2624 1870
Size: 754 4545
XmnI GAAnn'nnTTC
Cuts at: 3428 3428
Size: 5299
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3107 276
Size: 53 83 186 936 1573 2468
Fragments arranged by size:
2468 1573 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI