Restriction map for
pFN21AB3580
(Circular) (Six-base) MAPSORT of: pFN21AB3580.seq Check: 4834 from: 1 to: 6319
pFN21AB3580 6319 bp
With 182 enzymes: SgfI *
October 20, 2009 10:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4129 278
Size: 53 83 186 936 2593 2468
Fragments arranged by size:
2593 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3662 1937
Size: 1725 4594
Acc65I G'GTAC_C
Cuts at: 3640 3640
Size: 6319
AclI AA'CG_TT
Cuts at: 1563 4446 4819 1563
Size: 2883 373 3063
Fragments arranged by size:
3063 2883 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2454 2468 4382 5766 6034 1632
Size: 822 14 1914 1384 268 1917
Fragments arranged by size:
1917 1914 1384 822 268 14
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6122
AflIII A'CryG_T
Cuts at: 1203 5218 6195 1203
Size: 4015 977 1327
Fragments arranged by size:
4015 1327 977
AgeI A'CCGG_T
Cuts at: 5150 5150
Size: 6319
AhdI GACnn_n'nnGTC
Cuts at: 5048 5048
Size: 6319
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6319
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6319
AlwNI CAG_nnn'CTG
Cuts at: 2513 4062 5634 2513
Size: 1549 1572 3198
Fragments arranged by size:
3198 1572 1549
ApaI G_GGCC'C
Cuts at: 2836 2836
Size: 6319
ApaLI G'TGCA_C
Cuts at: 4376 5532 4376
Size: 1156 5163
ApoI r'AATT_y
Cuts at: 1493 2318 2476 2747 2868 3182 3628 3854 4006
Size: 825 158 271 121 314 446 226 152
Cuts at: 4006 6115 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 825 446 314 271 226 158
152 121
AseI AT'TA_AT
Cuts at: 160 1032 2220 4873 160
Size: 872 1188 2653 1606
Fragments arranged by size:
2653 1606 1188 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6319
AvaI C'yCGr_G
Cuts at: 1870 1943 3644 1870
Size: 73 1701 4545
Fragments arranged by size:
4545 1701 73
BamHI G'GATC_C
Cuts at: 3649 4016 3649
Size: 367 5952
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2236 3640 5095 618
Size: 325 217 627 449 1404 1455 1842
Fragments arranged by size:
1842 1455 1404 627 449 325 217
BanII G_rGCy'C
Cuts at: 1391 2581 2836 3638 1391
Size: 1190 255 802 4072
Fragments arranged by size:
4072 1190 802 255
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3537 928
Size: 971 1638 3710
Fragments arranged by size:
3710 1638 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4510 4544 1903
Size: 34 2573 34 3678
Fragments arranged by size:
3678 2573 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4211 5421 4211
Size: 1210 5109
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6319
BfrBI ATG'CAT
Cuts at: 2923 2923
Size: 6319
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2831 4930 136
Size: 107 122 71 631 605 1159 2099 1525
Fragments arranged by size:
2099 1525 1159 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3477 6314 3477
Size: 2837 3482
BlpI GC'TnA_GC
Cuts at: 2537 3016 3736 2537
Size: 479 720 5120
Fragments arranged by size:
5120 720 479
Bme1580I G_kGCm'C
Cuts at: 1790 2520 2836 3118 4380 5536 1790
Size: 730 316 282 1262 1156 2573
Fragments arranged by size:
2573 1262 1156 730 316 282
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6319
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3278 4050 5008
Size: 416 53 83 399 24 1861 772 958
Cuts at: 5008 442
Size: 1753
Fragments arranged by size:
1861 1753 958 772 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6319
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2616 2727 3252 4979 1239
Size: 1377 111 525 1727 2579
Fragments arranged by size:
2579 1727 1377 525 111
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4444 5309 5607 4444
Size: 865 298 5156
Fragments arranged by size:
5156 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4982 882
Size: 617 3483 2219
Fragments arranged by size:
3483 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4110 493
Size: 711 2906 2702
Fragments arranged by size:
2906 2702 711
BsaBI GATnn'nnATC
Cuts at: 6313 6313
Size: 6319
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4126 4508 5143 275
Size: 53 83 186 936 2593 382 635 1451
Fragments arranged by size:
2593 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4752 5150 5424 5571 5982 4752
Size: 398 274 147 411 5089
Fragments arranged by size:
5089 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6094
Fragments arranged by size:
6094 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2098 3287 1186
Size: 912 1189 4218
Fragments arranged by size:
4218 1189 912
BseYI C'CCAG_C
Cuts at: 2829 3151 5522 6018 2829
Size: 322 2371 496 3130
Fragments arranged by size:
3130 2371 496 322
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2632 1163
Size: 573 896 4850
Fragments arranged by size:
4850 896 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3769 4530 5558 1540
Size: 275 186 1768 761 1028 2301
Fragments arranged by size:
2301 1768 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4380 4465 5536 4380
Size: 85 1071 5163
Fragments arranged by size:
5163 1071 85
BsmI GAATG_Cn'
Cuts at: 2701 3830 3923 2701
Size: 1129 93 5097
Fragments arranged by size:
5097 1129 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2520 2581 2836 3118 3638 4380 4465
Size: 399 730 61 255 282 520 742 85
Cuts at: 4465 5536 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 730 520 399 282 255
85 61
BspEI T'CCGG_A
Cuts at: 5982 5982
Size: 6319
BspHI T'CATG_A
Cuts at: 2206 3463 4208 5938 2206
Size: 1257 745 1730 2587
Fragments arranged by size:
2587 1730 1257 745
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3674 844
Size: 2830 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3286 4206 1394
Size: 429 1463 920 3507
Fragments arranged by size:
3507 1463 920 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2589 3540 4814 4988 65
Size: 1183 1341 951 1274 174 1396
Fragments arranged by size:
1396 1341 1274 1183 951 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4963 5150 1700
Size: 110 3153 187 2869
Fragments arranged by size:
3153 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2426 3322 96
Size: 2330 896 3093
Fragments arranged by size:
3093 2330 896
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6319
BssSI C'ACGA_G
Cuts at: 4379 5391 4379
Size: 1012 5307
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6319
BstBI TT'CG_AA
Cuts at: 5123 5123
Size: 6319
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6319
BstXI CCAn_nnnn'nTGG
Cuts at: 2398 2398
Size: 6319
BstYI r'GATC_y
Cuts at: 1972 3120 3477 3649 4016 4401 4418 5870 5956
Size: 1148 357 172 367 385 17 1452 86
Cuts at: 5956 5968 6314 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1148 385 367 357 346 172
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3356 3356
Size: 6319
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5767
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5580
Fragments arranged by size:
5580 683 56
BtsI GCAGTG_nn'
Cuts at: 2429 3835 4629 4649 2429
Size: 1406 794 20 4099
Fragments arranged by size:
4099 1406 794 20
ClaI AT'CG_AT
Cuts at: 1554 2883 1554
Size: 1329 4990
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6284
DraI TTT'AAA
Cuts at: 3623 3623
Size: 6319
DraIII CAC_nnn'GTG
Cuts at: 2243 2243
Size: 6319
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5326 809
Size: 507 4010 1802
Fragments arranged by size:
4010 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2388 3766 4655
Size: 54 1425 50 275 18 558 1378 889
Cuts at: 4655 6284 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1378 889 558 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3766 1537
Size: 275 1954 4090
Fragments arranged by size:
4090 1954 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2121 2487 4249 1353
Size: 768 366 1762 3423
Fragments arranged by size:
3423 1762 768 366
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4906 5280 5426 387
Size: 1064 138 3317 374 146 1280
Fragments arranged by size:
3317 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2454 2468 2616 2727 3252 4382 4979
Size: 393 822 14 148 111 525 1130 597
Cuts at: 4979 5766 6034 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1130 822 787 597 525 393 268
148 111 14
EcoNI CCTnn'n_nnAGG
Cuts at: 3603 3603
Size: 6319
EcoO109I rG'GnC_Cy
Cuts at: 2833 2833
Size: 6319
EcoRI G'AATT_C
Cuts at: 3628 4006 3628
Size: 378 5941
EcoRV GAT'ATC
Cuts at: 3090 3090
Size: 6319
FspI TGC'GCA
Cuts at: 2078 4825 2078
Size: 2747 3572
HaeII r_GCGC'y
Cuts at: 1678 5466 1678
Size: 3788 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4960 4992 5034 5066 1373
Size: 32 135 32 3388 32 42 32 2626
Fragments arranged by size:
3388 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3556 3663 3909 669
Size: 988 281 1618 107 246 3079
Fragments arranged by size:
3079 1618 988 281 246 107
HindIII A'AGCT_T
Cuts at: 748 3426 748
Size: 2678 3641
HpaI GTT'AAC
Cuts at: 3909 3909
Size: 6319
KpnI G_GTAC'C
Cuts at: 3644 3644
Size: 6319
MfeI C'AATT_G
Cuts at: 3918 3918
Size: 6319
MluI A'CGCG_T
Cuts at: 6195 6195
Size: 6319
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3062 3607 5433 5617 1305
Size: 1757 545 1826 184 2007
Fragments arranged by size:
2007 1826 1757 545 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2390 10
Size: 54 1425 343 558 3939
Fragments arranged by size:
3939 1425 558 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2231 3305 4277 4636 4795 518
Size: 648 1065 1074 972 359 159 2042
Fragments arranged by size:
2042 1074 1065 972 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3104 3242 3735 4412 5560 5805 1606
Size: 1498 138 493 677 1148 245 2120
Fragments arranged by size:
2120 1498 1148 677 493 245 138
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5767
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6319
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6319
NotI GC'GGCC_GC
Cuts at: 3766 3766
Size: 6319
NsiI A_TGCA'T
Cuts at: 2925 2925
Size: 6319
NspI r_CATG'y
Cuts at: 1132 1229 3081 3677 5222 1132
Size: 97 1852 596 1545 2229
Fragments arranged by size:
2229 1852 1545 596 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6319
PciI A'CATG_T
Cuts at: 5218 5218
Size: 6319
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6319
PfoI T'CCnGG_A
Cuts at: 2559 2559
Size: 6319
PmeI GTTT'AAAC
Cuts at: 3623 3623
Size: 6319
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4388 4420 5901 5933 4388
Size: 32 1481 32 4774
Fragments arranged by size:
4774 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6319
PsiI TTA'TAA
Cuts at: 2934 3889 2934
Size: 955 5364
PspOMI G'GGCC_C
Cuts at: 2832 2832
Size: 6319
PstI C_TGCA'G
Cuts at: 830 2106 2436 3027 3671 830
Size: 1276 330 591 644 3478
Fragments arranged by size:
3478 1276 644 591 330
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6319
PvuII CAG'CTG
Cuts at: 3104 3242 3104
Size: 138 6181
SalI G'TCGA_C
Cuts at: 1936 3661 1936
Size: 1725 4594
SapI GCTCTTCn'nnn_
Cuts at: 2121 2121
Size: 6319
SbfI CC_TGCA'GG
Cuts at: 3671 3671
Size: 6319
ScaI AGT'ACT
Cuts at: 4567 6279 4567
Size: 1712 4607
SexAI A'CCwGG_T
Cuts at: 2239 2239
Size: 6319
SfcI C'TryA_G
Cuts at: 826 1045 2102 2157 2432 2722 3023 3667 4802
Size: 219 1057 55 275 290 301 644 1135
Cuts at: 4802 5483 5674 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1057 681 644 301 290 275
219 191 55
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6319
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6319
SmaI CCC'GGG
Cuts at: 1872 3646 1872
Size: 1774 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4423 5324 5586 820
Size: 197 926 2480 901 262 1553
Fragments arranged by size:
2480 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4110 493
Size: 3617 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6319
SphI G_CATG'C
Cuts at: 1132 3677 1132
Size: 2545 3774
SspI AAT'ATT
Cuts at: 5 52 2464 3097 4243 5
Size: 47 2412 633 1146 2081
Fragments arranged by size:
2412 2081 1146 633 47
StuI AGG'CCT
Cuts at: 1517 3491 1517
Size: 1974 4345
StyI C'CwwG_G
Cuts at: 513 1065 3758 513
Size: 552 2693 3074
Fragments arranged by size:
3074 2693 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3536 4346 4363 4516 4701 5146 325
Size: 1255 1956 810 17 153 185 445 1498
Fragments arranged by size:
1956 1498 1255 810 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2426 2812 3322
Size: 274 80 33 51 1443 449 386 510
Cuts at: 3322 3573 4565 6277 96
Size: 251 992 1712 138
Fragments arranged by size:
1712 1443 992 510 449 386 274 251
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6279 6311 6279
Size: 32 6287
Tth111I GACn'n_nGTC
Cuts at: 1316 2621 1316
Size: 1305 5014
XbaI T'CTAG_A
Cuts at: 3655 3655
Size: 6319
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6319
XmaI C'CCGG_G
Cuts at: 1870 3644 1870
Size: 1774 4545
XmnI GAAnn'nnTTC
Cuts at: 4448 4448
Size: 6319
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4127 276
Size: 53 83 186 936 2593 2468
Fragments arranged by size:
2593 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstZ17I EcoICRI FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PpuMI PspXI PsrI RsrII SacI SacII SanDI SfiI
SfoI SrfI SwaI XcmI