Restriction map for
pFN21AB3524
(Circular) (Six-base) MAPSORT of: pFN21AB3524.seq Check: 7723 from: 1 to: 6085
pFN21AB3524 6085 bp
With 182 enzymes: SgfI *
October 20, 2009 10:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2461 3895 278
Size: 53 83 186 936 925 1434 2468
Fragments arranged by size:
2468 1434 936 925 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2314 3428 1937
Size: 377 1114 4594
Fragments arranged by size:
4594 1114 377
Acc65I G'GTAC_C
Cuts at: 3406 3406
Size: 6085
AclI AA'CG_TT
Cuts at: 1563 2754 4212 4585 1563
Size: 1191 1458 373 3063
Fragments arranged by size:
3063 1458 1191 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2822 2948 3111 4148 5532 5800 1632
Size: 1190 126 163 1037 1384 268 1917
Fragments arranged by size:
1917 1384 1190 1037 268 163 126
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5888
AflIII A'CryG_T
Cuts at: 1203 4984 5961 1203
Size: 3781 977 1327
Fragments arranged by size:
3781 1327 977
AgeI A'CCGG_T
Cuts at: 4916 4916
Size: 6085
AhdI GACnn_n'nnGTC
Cuts at: 4814 4814
Size: 6085
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6085
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6085
AlwNI CAG_nnn'CTG
Cuts at: 2519 2840 3828 5400 2519
Size: 321 988 1572 3204
Fragments arranged by size:
3204 1572 988 321
ApaLI G'TGCA_C
Cuts at: 4142 5298 4142
Size: 1156 4929
ApoI r'AATT_y
Cuts at: 1493 2387 2399 2721 2867 3394 3620 3772 5881
Size: 894 12 322 146 527 226 152 2109
Cuts at: 5881 1493
Size: 1697
Fragments arranged by size:
2109 1697 894 527 322 226 152 146
12
AseI AT'TA_AT
Cuts at: 160 1032 4639 160
Size: 872 3607 1606
Fragments arranged by size:
3607 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6085
AvaI C'yCGr_G
Cuts at: 1870 1943 2084 3410 1870
Size: 73 141 1326 4545
Fragments arranged by size:
4545 1326 141 73
BamHI G'GATC_C
Cuts at: 2710 3415 3782 2710
Size: 705 367 5013
Fragments arranged by size:
5013 705 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3406 4861 618
Size: 325 217 627 1619 1455 1842
Fragments arranged by size:
1842 1619 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2085 2850 3404 1391
Size: 694 765 554 4072
Fragments arranged by size:
4072 765 694 554
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3378 928
Size: 971 1479 3635
Fragments arranged by size:
3635 1479 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4276 4310 1903
Size: 34 2339 34 3678
Fragments arranged by size:
3678 2339 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3977 5187 3977
Size: 1210 4875
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6085
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4696 136
Size: 107 122 71 631 605 3024 1525
Fragments arranged by size:
3024 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6080 6080
Size: 6085
BlpI GC'TnA_GC
Cuts at: 3502 3502
Size: 6085
Bme1580I G_kGCm'C
Cuts at: 1790 4146 5302 1790
Size: 2356 1156 2573
Fragments arranged by size:
2573 2356 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6085
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3816 4774 442
Size: 416 53 83 399 24 2399 958 1753
Fragments arranged by size:
2399 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2942 1056
Size: 1886 4199
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2117 2706 4745 1239
Size: 878 589 2039 2579
Fragments arranged by size:
2579 2039 878 589
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3377 4210 5075 5373 3377
Size: 833 865 298 4089
Fragments arranged by size:
4089 865 833 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4748 882
Size: 617 3249 2219
Fragments arranged by size:
3249 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3194 3876 493
Size: 711 1990 682 2702
Fragments arranged by size:
2702 1990 711 682
BsaBI GATnn'nnATC
Cuts at: 2955 6079 2955
Size: 3124 2961
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2458 3892 4274 4909
Size: 53 83 186 936 925 1434 382 635
Cuts at: 4909 275
Size: 1451
Fragments arranged by size:
1451 1434 936 925 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4518 4916 5190 5337 5748 4518
Size: 398 274 147 411 4855
Fragments arranged by size:
4855 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2752 2782 1178
Size: 30 165 30 1349 30 4481
Fragments arranged by size:
4481 1349 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2585 2660 2663 2666 2669 2672 2675 2678
Size: 1399 75 3 3 3 3 3 3
Cuts at: 2678 2720 3120 1186
Size: 42 400 4151
Fragments arranged by size:
4151 1399 400 75 42 3 3 3
3 3 3
BseYI C'CCAG_C
Cuts at: 2120 2162 2204 5288 5784 2120
Size: 42 42 3084 496 2421
Fragments arranged by size:
3084 2421 496 42 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5512
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3535 4296 5324 1540
Size: 275 186 1534 761 1028 2301
Fragments arranged by size:
2301 1534 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4146 4231 5302 4146
Size: 85 1071 4929
Fragments arranged by size:
4929 1071 85
BsmI GAATG_Cn'
Cuts at: 3596 3689 3596
Size: 93 5992
BsmBI CGTCTCn'nnnn_
Cuts at: 2124 2124
Size: 6085
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2085 2850 3404 4146 4231 5302 1391
Size: 399 295 765 554 742 85 1071 2174
Fragments arranged by size:
2174 1071 765 742 554 399 295 85
BspEI T'CCGG_A
Cuts at: 5748 5748
Size: 6085
BspHI T'CATG_A
Cuts at: 2404 2548 3974 5704 2404
Size: 144 1426 1730 2785
Fragments arranged by size:
2785 1730 1426 144
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3440 844
Size: 2596 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2369 3972 1394
Size: 429 546 1603 3507
Fragments arranged by size:
3507 1603 546 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4580 4754 65
Size: 1183 3332 174 1396
Fragments arranged by size:
3332 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4729 4916 1700
Size: 110 2919 187 2869
Fragments arranged by size:
2919 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6085
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6085
BssSI C'ACGA_G
Cuts at: 4145 5157 4145
Size: 1012 5073
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6085
BstBI TT'CG_AA
Cuts at: 4889 4889
Size: 6085
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6085
BstYI r'GATC_y
Cuts at: 1972 2710 2836 3063 3415 3782 4167 4184 5636
Size: 738 126 227 352 367 385 17 1452
Cuts at: 5636 5722 5734 6080 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 738 385 367 352 346 227
126 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2393 2715 2393
Size: 322 5763
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4594
Fragments arranged by size:
4594 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5346
Fragments arranged by size:
5346 683 56
BtsI GCAGTG_nn'
Cuts at: 3601 4395 4415 3601
Size: 794 20 5271
Fragments arranged by size:
5271 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6085
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6050
DraI TTT'AAA
Cuts at: 2748 3389 2748
Size: 641 5444
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5092 809
Size: 507 3776 1802
Fragments arranged by size:
3776 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2370 3532
Size: 54 1425 50 275 18 177 363 1162
Cuts at: 3532 4421 6050 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1162 889 363 275 177 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3532 1537
Size: 275 1720 4090
Fragments arranged by size:
4090 1720 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2046 2487 2547 2637 2685 2697 4015 1353
Size: 693 441 60 90 48 12 1318 3423
Fragments arranged by size:
3423 1318 693 441 90 60 48 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4672 5046 5192 387
Size: 1064 138 3083 374 146 1280
Fragments arranged by size:
3083 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2117 2706 2822 2948 3111 4148 4745
Size: 393 485 589 116 126 163 1037 597
Cuts at: 4745 5532 5800 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1037 787 597 589 485 393 268
163 126 116
EcoRI G'AATT_C
Cuts at: 2721 3394 3772 2721
Size: 673 378 5034
Fragments arranged by size:
5034 673 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2846 2846
Size: 6085
FspI TGC'GCA
Cuts at: 4591 4591
Size: 6085
HaeII r_GCGC'y
Cuts at: 1678 5232 1678
Size: 3554 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2234 2266 2448 2480 4726
Size: 32 135 32 662 32 182 32 2246
Cuts at: 4726 4758 4800 4832 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2246 662 182 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2910 3429 3675 669
Size: 988 281 972 519 246 3079
Fragments arranged by size:
3079 988 972 519 281 246
HindIII A'AGCT_T
Cuts at: 748 2354 2828 3339 748
Size: 1606 474 511 3494
Fragments arranged by size:
3494 1606 511 474
HpaI GTT'AAC
Cuts at: 3675 3675
Size: 6085
KpnI G_GTAC'C
Cuts at: 3410 3410
Size: 6085
MfeI C'AATT_G
Cuts at: 3324 3684 3324
Size: 360 5725
MluI A'CGCG_T
Cuts at: 5961 5961
Size: 6085
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5199 5383 1305
Size: 3894 184 2007
Fragments arranged by size:
3894 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4263
Fragments arranged by size:
4263 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2589 4043 4402 4561 518
Size: 648 1423 1454 359 159 2042
Fragments arranged by size:
2042 1454 1423 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2117 2162 3501 4178 5326 5571 1606
Size: 511 45 1339 677 1148 245 2120
Fragments arranged by size:
2120 1339 1148 677 511 245 45
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4594
Fragments arranged by size:
4594 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6085
NheI G'CTAG_C
Cuts at: 1052 2938 1052
Size: 1886 4199
NotI GC'GGCC_GC
Cuts at: 3532 3532
Size: 6085
NspI r_CATG'y
Cuts at: 1132 1229 3443 4988 1132
Size: 97 2214 1545 2229
Fragments arranged by size:
2229 2214 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6085
PciI A'CATG_T
Cuts at: 4984 4984
Size: 6085
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6085
PmeI GTTT'AAAC
Cuts at: 3389 3389
Size: 6085
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4154 4186 5667 5699 4154
Size: 32 1481 32 4540
Fragments arranged by size:
4540 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6085
PsiI TTA'TAA
Cuts at: 3655 3655
Size: 6085
PstI C_TGCA'G
Cuts at: 830 3437 830
Size: 2607 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6085
SalI G'TCGA_C
Cuts at: 1936 3427 1936
Size: 1491 4594
SbfI CC_TGCA'GG
Cuts at: 3437 3437
Size: 6085
ScaI AGT'ACT
Cuts at: 4333 6045 4333
Size: 1712 4373
SfcI C'TryA_G
Cuts at: 826 1045 2487 2962 3433 4568 5249 5440 826
Size: 219 1442 475 471 1135 681 191 1471
Fragments arranged by size:
1471 1442 1135 681 475 471 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6085
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6085
SmaI CCC'GGG
Cuts at: 1872 3412 1872
Size: 1540 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3356 4189 5090 5352 820
Size: 197 926 1413 833 901 262 1553
Fragments arranged by size:
1553 1413 926 901 833 262 197
SnaBI TAC'GTA
Cuts at: 493 3194 3876 493
Size: 2701 682 2702
Fragments arranged by size:
2702 2701 682
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6085
SphI G_CATG'C
Cuts at: 1132 3443 1132
Size: 2311 3774
SspI AAT'ATT
Cuts at: 5 52 2891 4009 5
Size: 47 2839 1118 2081
Fragments arranged by size:
2839 2081 1118 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6085
StyI C'CwwG_G
Cuts at: 513 1065 2004 3102 3524 513
Size: 552 939 1098 422 3074
Fragments arranged by size:
3074 1098 939 552 422
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4112 4129 4282 4467 4912 325
Size: 1255 2532 17 153 185 445 1498
Fragments arranged by size:
2532 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4331 6043 96
Size: 274 80 33 51 1443 2354 1712 138
Fragments arranged by size:
2354 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2131 2163 2215 2247 6045 6077 2131
Size: 32 52 32 3798 32 2139
Fragments arranged by size:
3798 2139 52 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6085
XbaI T'CTAG_A
Cuts at: 3421 3421
Size: 6085
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6085
XmaI C'CCGG_G
Cuts at: 1870 3410 1870
Size: 1540 4545
XmnI GAAnn'nnTTC
Cuts at: 3096 4214 3096
Size: 1118 4967
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2459 3893 276
Size: 53 83 186 936 925 1434 2468
Fragments arranged by size:
2468 1434 936 925 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I DraIII
EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI