Restriction map for
pFN21AB3523
(Circular) (Six-base) MAPSORT of: pFN21AB3523.seq Check: 646 from: 1 to: 7279
pFN21AB3523 7279 bp
With 182 enzymes: SgfI *
February 27, 2009 14:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2518 2518
Size: 7279
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5089 278
Size: 53 83 186 936 3553 2468
Fragments arranged by size:
3553 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2211 4035 4622 1937
Size: 274 1824 587 4594
Fragments arranged by size:
4594 1824 587 274
Acc65I G'GTAC_C
Cuts at: 4600 4600
Size: 7279
AclI AA'CG_TT
Cuts at: 1563 5406 5779 1563
Size: 3843 373 3063
Fragments arranged by size:
3843 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2530 2876 3022 3574 4267 4329 5342 6726
Size: 898 346 146 552 693 62 1013 1384
Cuts at: 6726 6994 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1013 898 693 552 346 268
146 62
AfeI AGC'GCT
Cuts at: 4236 4236
Size: 7279
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7082
AflIII A'CryG_T
Cuts at: 1203 2148 2775 6178 7155 1203
Size: 945 627 3403 977 1327
Fragments arranged by size:
3403 1327 977 945 627
AgeI A'CCGG_T
Cuts at: 6110 6110
Size: 7279
AhdI GACnn_n'nnGTC
Cuts at: 4382 6008 4382
Size: 1626 5653
AleI CACnn'nnGTG
Cuts at: 1166 3131 1166
Size: 1965 5314
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2867 3324 3668 3839 1184
Size: 1683 457 344 171 4624
Fragments arranged by size:
4624 1683 457 344 171
AlwNI CAG_nnn'CTG
Cuts at: 2267 3671 5022 6594 2267
Size: 1404 1351 1572 2952
Fragments arranged by size:
2952 1572 1404 1351
ApaI G_GGCC'C
Cuts at: 3580 3619 3580
Size: 39 7240
ApaLI G'TGCA_C
Cuts at: 5336 6492 5336
Size: 1156 6123
ApoI r'AATT_y
Cuts at: 1493 3284 4588 4814 4966 7075 1493
Size: 1791 1304 226 152 2109 1697
Fragments arranged by size:
2109 1791 1697 1304 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5833 160
Size: 872 4801 1606
Fragments arranged by size:
4801 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7279
AvaI C'yCGr_G
Cuts at: 1870 1943 2400 4215 4604 1870
Size: 73 457 1815 389 4545
Fragments arranged by size:
4545 1815 457 389 73
BamHI G'GATC_C
Cuts at: 4609 4976 4609
Size: 367 6912
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2235 2750 3208 3583 3751
Size: 325 217 627 448 515 458 375 168
Cuts at: 3751 4436 4600 6055 618
Size: 685 164 1455 1842
Fragments arranged by size:
1842 1455 685 627 515 458 448 375
325 217 168 164
BanII G_rGCy'C
Cuts at: 1391 3365 3580 3619 3795 3885 4148 4598 1391
Size: 1974 215 39 176 90 263 450 4072
Fragments arranged by size:
4072 1974 450 263 215 176 90 39
BbeI G_GCGC'C
Cuts at: 2239 2239
Size: 7279
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2873 3921 3927 928
Size: 971 974 1048 6 4280
Fragments arranged by size:
4280 1048 974 971 6
BbvCI CC'TCA_GC
Cuts at: 4059 4059
Size: 7279
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4286 4320 5470 5504 1903
Size: 34 2349 34 1150 34 3678
Fragments arranged by size:
3678 2349 1150 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5171 6381 5171
Size: 1210 6069
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7279
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2296 3614 3656
Size: 107 122 71 631 605 624 1318 42
Cuts at: 3656 5890 136
Size: 2234 1525
Fragments arranged by size:
2234 1525 1318 631 624 605 122 107
71 42
BglII A'GATC_T
Cuts at: 3084 7274 3084
Size: 4190 3089
BlpI GC'TnA_GC
Cuts at: 2823 4339 4696 2823
Size: 1516 357 5406
Fragments arranged by size:
5406 1516 357
Bme1580I G_kGCm'C
Cuts at: 1790 2753 3480 3580 3588 3619 3756 3818 4439
Size: 963 727 100 8 31 137 62 621
Cuts at: 4439 5340 6496 1790
Size: 901 1156 2573
Fragments arranged by size:
2573 1156 963 901 727 621 137 100
62 31 8
BmgBI CAC'GTC
Cuts at: 1318 2776 1318
Size: 1458 5821
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2420 3757 3799
Size: 416 53 83 399 24 1003 1337 42
Cuts at: 3799 4084 5010 5968 442
Size: 285 926 958 1753
Fragments arranged by size:
1753 1337 1003 958 926 416 399 285
83 53 42 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7279
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4354 4354
Size: 7279
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2088 2289 2411 3098 3533 3623 3661 5939
Size: 849 201 122 687 435 90 38 2278
Cuts at: 5939 1239
Size: 2579
Fragments arranged by size:
2579 2278 849 687 435 201 122 90
38
Bpu10I CC'TnA_GC
Cuts at: 2330 3621 4059 2330
Size: 1291 438 5550
Fragments arranged by size:
5550 1291 438
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2537 2939 5404 6269 6567 2537
Size: 402 2465 865 298 3249
Fragments arranged by size:
3249 2465 865 402 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2064 2265 5942 882
Size: 617 565 201 3677 2219
Fragments arranged by size:
3677 2219 617 565 201
BsaAI yAC'GTr
Cuts at: 493 1204 5070 493
Size: 711 3866 2702
Fragments arranged by size:
3866 2702 711
BsaBI GATnn'nnATC
Cuts at: 7273 7273
Size: 7279
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2236 4017 5086 5468
Size: 53 83 186 936 703 1781 1069 382
Cuts at: 5468 6103 275
Size: 635 1451
Fragments arranged by size:
1781 1451 1069 936 703 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2988 3184 3874 5712 6110 6384 6531 6942 2988
Size: 196 690 1838 398 274 147 411 3325
Fragments arranged by size:
3325 1838 690 411 398 274 196 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2061 2091 2468 2498 2760
Size: 30 165 30 658 30 377 30 262
Cuts at: 2760 2790 3908 3938 1178
Size: 30 1118 30 4519
Fragments arranged by size:
4519 1118 658 377 262 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2036 2261 2351 3518 3536 3560 3803 3851
Size: 850 225 90 1167 18 24 243 48
Cuts at: 3851 3854 3937 1186
Size: 3 83 4528
Fragments arranged by size:
4528 1167 850 243 225 90 83 48
24 18 3
BseYI C'CCAG_C
Cuts at: 2746 2813 3142 3444 6482 6978 2746
Size: 67 329 302 3038 496 3047
Fragments arranged by size:
3047 3038 496 329 302 67
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2472 2522 1163
Size: 573 736 50 5920
Fragments arranged by size:
5920 736 573 50
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2475 4729 5490 6518 1540
Size: 275 186 474 2254 761 1028 2301
Fragments arranged by size:
2301 2254 1028 761 474 275 186
BsiHKAI G_wGCw'C
Cuts at: 2603 2712 2829 3036 3365 3647 3795 5340 5425
Size: 109 117 207 329 282 148 1545 85
Cuts at: 5425 6496 2603
Size: 1071 3386
Fragments arranged by size:
3386 1545 1071 329 282 207 148 117
109 85
BsmI GAATG_Cn'
Cuts at: 3100 4790 4883 3100
Size: 1690 93 5496
Fragments arranged by size:
5496 1690 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2603 2712 2753 2829 3036 3365 3480
Size: 399 813 109 41 76 207 329 115
Cuts at: 3480 3580 3588 3619 3647 3756 3795 3818 3885
Size: 100 8 31 28 109 39 23 67
Cuts at: 3885 4148 4439 4598 5340 5425 6496 1391
Size: 263 291 159 742 85 1071 2174
Fragments arranged by size:
2174 1071 813 742 399 329 291 263
207 159 115 109 109 100 85 76
67 41 39 31 28 23 8
BspEI T'CCGG_A
Cuts at: 2988 3874 6942 2988
Size: 886 3068 3325
Fragments arranged by size:
3325 3068 886
BspHI T'CATG_A
Cuts at: 5168 6898 5168
Size: 1730 5549
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2518 3616 4634 844
Size: 1674 1098 1018 3489
Fragments arranged by size:
3489 1674 1098 1018
BsrBI CCG'CTC
Cuts at: 1394 1823 3205 4338 5166 1394
Size: 429 1382 1133 828 3507
Fragments arranged by size:
3507 1382 1133 828 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3237 3852 5774 5948 65
Size: 1183 1989 615 1922 174 1396
Fragments arranged by size:
1989 1922 1396 1183 615 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2198 3297 5923 6110 1700
Size: 110 388 1099 2626 187 2869
Fragments arranged by size:
2869 2626 1099 388 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7279
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7279
BssSI C'ACGA_G
Cuts at: 5339 6351 5339
Size: 1012 6267
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2500 794
Size: 1706 5573
BstBI TT'CG_AA
Cuts at: 6083 6083
Size: 7279
BstEII G'GTnAC_C
Cuts at: 1184 2443 1184
Size: 1259 6020
BstXI CCAn_nnnn'nTGG
Cuts at: 2299 2455 2915 3763 4543 2299
Size: 156 460 848 780 5035
Fragments arranged by size:
5035 848 780 460 156
BstYI r'GATC_y
Cuts at: 1972 3084 4278 4609 4976 5361 5378 6830 6916
Size: 1112 1194 331 367 385 17 1452 86
Cuts at: 6916 6928 7274 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1194 1112 385 367 346 331
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2061 4176 2061
Size: 2115 5164
BtgI C'CryG_G
Cuts at: 513 1065 2004 3148 3480 4203 4317 513
Size: 552 939 1144 332 723 114 3475
Fragments arranged by size:
3475 1144 939 723 552 332 114
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3210 3942 487
Size: 683 56 1984 732 3824
Fragments arranged by size:
3824 1984 732 683 56
BtsI GCAGTG_nn'
Cuts at: 2460 2689 3557 4795 5589 5609 2460
Size: 229 868 1238 794 20 4130
Fragments arranged by size:
4130 1238 868 794 229 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7279
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3670 3705 529
Size: 35 3106 35 4103
Fragments arranged by size:
4103 3106 35 35
DraI TTT'AAA
Cuts at: 4583 4583
Size: 7279
DraIII CAC_nnn'GTG
Cuts at: 2704 2704
Size: 7279
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3710 6286 809
Size: 507 2394 2576 1802
Fragments arranged by size:
2576 2394 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2200 2472 3135
Size: 54 1425 50 275 18 370 272 663
Cuts at: 3135 3420 4572 4726 5615 7244 8
Size: 285 1152 154 889 1629 43
Fragments arranged by size:
1629 1425 1152 889 663 370 285 275
272 154 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2472 4726 1537
Size: 275 660 2254 4090
Fragments arranged by size:
4090 2254 660 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3041 3537 5209 1353
Size: 1688 496 1672 3423
Fragments arranged by size:
3423 1688 1672 496
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 2242 2334 5866 6240 6386
Size: 1064 138 435 218 92 3532 374 146
Cuts at: 6386 387
Size: 1280
Fragments arranged by size:
3532 1280 1064 435 374 218 146 138
92
EcoICRI GAG'CTC
Cuts at: 3363 3793 3363
Size: 430 6849
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2088 2289 2411 2530 2876 3022 3098
Size: 393 456 201 122 119 346 146 76
Cuts at: 3098 3533 3574 3623 3661 4267 4329 5342 5939
Size: 435 41 49 38 606 62 1013 597
Cuts at: 5939 6726 6994 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1013 787 606 597 456 435 393
346 268 201 146 122 119 76 62
49 41 38
EcoNI CCTnn'n_nnAGG
Cuts at: 2259 2333 2259
Size: 74 7205
EcoO109I rG'GnC_Cy
Cuts at: 2056 2128 2251 2461 3259 3615 3616 4376 4540
Size: 72 123 210 798 356 1 760 164
Cuts at: 4540 2056
Size: 4795
Fragments arranged by size:
4795 798 760 356 210 164 123 72
1
EcoRI G'AATT_C
Cuts at: 3284 4588 4966 3284
Size: 1304 378 5597
Fragments arranged by size:
5597 1304 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2560 3524 3751 3911 2560
Size: 964 227 160 5928
Fragments arranged by size:
5928 964 227 160
FspI TGC'GCA
Cuts at: 5785 5785
Size: 7279
HaeII r_GCGC'y
Cuts at: 1678 2030 2239 4238 6426 1678
Size: 352 209 1999 2188 2531
Fragments arranged by size:
2531 2188 1999 352 209
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2061 2093 2468 2500 2765
Size: 32 135 32 489 32 375 32 265
Cuts at: 2765 2797 2816 2848 3908 3940 4270 4302 4340
Size: 32 19 32 1060 32 330 32 38
Cuts at: 4340 4368 4372 4400 4479 4511 5920 5952 5994
Size: 28 4 28 79 32 1409 32 42
Cuts at: 5994 6026 1373
Size: 32 2626
Fragments arranged by size:
2626 1409 1060 489 375 330 265 135
79 42 38 32 32 32 32 32
32 32 32 32 32 32 28 28
19 4
HincII GTy'rAC
Cuts at: 669 1657 1938 3454 4623 4869 669
Size: 988 281 1516 1169 246 3079
Fragments arranged by size:
3079 1516 1169 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7279
HpaI GTT'AAC
Cuts at: 4869 4869
Size: 7279
KasI G'GCGC_C
Cuts at: 2235 2235
Size: 7279
KpnI G_GTAC'C
Cuts at: 4604 4604
Size: 7279
MfeI C'AATT_G
Cuts at: 4878 4878
Size: 7279
MluI A'CGCG_T
Cuts at: 7155 7155
Size: 7279
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2541 2871 3977 6393 6577 1305
Size: 1236 330 1106 2416 184 2007
Fragments arranged by size:
2416 2007 1236 1106 330 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3137 3422 4574 10
Size: 54 1425 343 1305 285 1152 2715
Fragments arranged by size:
2715 1425 1305 1152 343 285 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2141 2849 3131 3779 4092 5237 5596
Size: 648 975 708 282 648 313 1145 359
Cuts at: 5596 5755 518
Size: 159 2042
Fragments arranged by size:
2042 1145 975 708 648 648 359 313
282 159
MspA1I CmG'CkG
Cuts at: 1606 2911 3006 3469 3991 4053 4343 4695 5372
Size: 1305 95 463 522 62 290 352 677
Cuts at: 5372 6520 6765 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1305 1148 677 522 463 352 290
245 95 62
NarI GG'CG_CC
Cuts at: 2236 2236
Size: 7279
NcoI C'CATG_G
Cuts at: 513 1065 2004 3148 3480 4203 4317 513
Size: 552 939 1144 332 723 114 3475
Fragments arranged by size:
3475 1144 939 723 552 332 114
NdeI CA'TA_TG
Cuts at: 387 2785 387
Size: 2398 4881
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7279
NotI GC'GGCC_GC
Cuts at: 4726 4726
Size: 7279
NspI r_CATG'y
Cuts at: 1132 1229 2543 2854 4637 6182 1132
Size: 97 1314 311 1783 1545 2229
Fragments arranged by size:
2229 1783 1545 1314 311 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7279
PciI A'CATG_T
Cuts at: 6178 6178
Size: 7279
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2704 3994 4074 4570 1108
Size: 1596 1290 80 496 3817
Fragments arranged by size:
3817 1596 1290 496 80
PfoI T'CCnGG_A
Cuts at: 2805 2934 4386 4396 2805
Size: 129 1452 10 5688
Fragments arranged by size:
5688 1452 129 10
PmeI GTTT'AAAC
Cuts at: 4583 4583
Size: 7279
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5348 5380 6861 6893 5348
Size: 32 1481 32 5734
Fragments arranged by size:
5734 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2056 2251 4376 4540 2056
Size: 195 2125 164 4795
Fragments arranged by size:
4795 2125 195 164
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7279
PsiI TTA'TAA
Cuts at: 4849 4849
Size: 7279
PspOMI G'GGCC_C
Cuts at: 3576 3615 3576
Size: 39 7240
PstI C_TGCA'G
Cuts at: 830 3657 3828 4631 830
Size: 2827 171 803 3478
Fragments arranged by size:
3478 2827 803 171
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7279
PvuII CAG'CTG
Cuts at: 3006 3469 3991 4343 3006
Size: 463 522 352 5942
Fragments arranged by size:
5942 522 463 352
SacI G_AGCT'C
Cuts at: 3365 3795 3365
Size: 430 6849
SalI G'TCGA_C
Cuts at: 1936 4621 1936
Size: 2685 4594
SanDI GG'GwC_CC
Cuts at: 2056 2056
Size: 7279
SapI GCTCTTCn'nnn_
Cuts at: 3041 3041
Size: 7279
SbfI CC_TGCA'GG
Cuts at: 3657 4631 3657
Size: 974 6305
ScaI AGT'ACT
Cuts at: 5527 7239 5527
Size: 1712 5567
SexAI A'CCwGG_T
Cuts at: 3708 3708
Size: 7279
SfcI C'TryA_G
Cuts at: 826 1045 2259 3653 3824 4627 5762 6443 6634
Size: 219 1214 1394 171 803 1135 681 191
Cuts at: 6634 826
Size: 1471
Fragments arranged by size:
1471 1394 1214 1135 803 681 219 191
171
SfiI GGCCn_nnn'nGGCC
Cuts at: 3614 3656 3614
Size: 42 7237
SfoI GGC'GCC
Cuts at: 2237 2237
Size: 7279
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7279
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7279
SmaI CCC'GGG
Cuts at: 1872 2402 4217 4606 1872
Size: 530 1815 389 4545
Fragments arranged by size:
4545 1815 530 389
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2552 2954 5383 6284 6546 820
Size: 197 926 609 402 2429 901 262 1553
Fragments arranged by size:
2429 1553 926 901 609 402 262 197
SnaBI TAC'GTA
Cuts at: 493 5070 493
Size: 4577 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7279
SphI G_CATG'C
Cuts at: 1132 2543 4637 1132
Size: 1411 2094 3774
Fragments arranged by size:
3774 2094 1411
SspI AAT'ATT
Cuts at: 5 52 5203 5
Size: 47 5151 2081
Fragments arranged by size:
5151 2081 47
StuI AGG'CCT
Cuts at: 1517 3608 4226 1517
Size: 2091 618 4570
Fragments arranged by size:
4570 2091 618
StyI C'CwwG_G
Cuts at: 513 1065 2004 3148 3480 3895 4203 4317 4536
Size: 552 939 1144 332 415 308 114 219
Cuts at: 4536 4718 513
Size: 182 3074
Fragments arranged by size:
3074 1144 939 552 415 332 308 219
182 114
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3143 3458 3739 4480 5306 5323 5476
Size: 1255 1563 315 281 741 826 17 153
Cuts at: 5476 5661 6106 325
Size: 185 445 1498
Fragments arranged by size:
1563 1498 1255 826 741 445 315 281
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2778 3438 5525
Size: 274 80 33 51 1443 801 660 2087
Cuts at: 5525 7237 96
Size: 1712 138
Fragments arranged by size:
2087 1712 1443 801 660 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2760 2792 7239 7271 2760
Size: 32 4447 32 2768
Fragments arranged by size:
4447 2768 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2075 2429 2801 1316
Size: 759 354 372 5794
Fragments arranged by size:
5794 759 372 354
XbaI T'CTAG_A
Cuts at: 4615 4615
Size: 7279
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4201 4201
Size: 7279
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7279
XmaI C'CCGG_G
Cuts at: 1870 2400 4215 4604 1870
Size: 530 1815 389 4545
Fragments arranged by size:
4545 1815 530 389
XmnI GAAnn'nnTTC
Cuts at: 3086 3570 5408 3086
Size: 484 1838 4957
Fragments arranged by size:
4957 1838 484
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5087 276
Size: 53 83 186 936 3553 2468
Fragments arranged by size:
3553 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I
EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI
PmlI PspXI PsrI RsrII SacII SrfI SwaI