Restriction map for
pFN21AB3518
(Circular) (Six-base) MAPSORT of: pFN21AB3518.seq Check: 5326 from: 1 to: 6985
pFN21AB3518 6985 bp
With 182 enzymes: SgfI *
October 20, 2009 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4082 4082
Size: 6985
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3183 4795 278
Size: 53 83 186 936 1647 1612 2468
Fragments arranged by size:
2468 1647 1612 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4328 1937
Size: 2391 4594
Acc65I G'GTAC_C
Cuts at: 4306 4306
Size: 6985
AclI AA'CG_TT
Cuts at: 1563 5112 5485 1563
Size: 3549 373 3063
Fragments arranged by size:
3549 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2288 2399 2429 3664 5048 6432 6700 1632
Size: 656 111 30 1235 1384 1384 268 1917
Fragments arranged by size:
1917 1384 1384 1235 656 268 111 30
AfeI AGC'GCT
Cuts at: 2771 2771
Size: 6985
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6788
AflIII A'CryG_T
Cuts at: 1203 5884 6861 1203
Size: 4681 977 1327
Fragments arranged by size:
4681 1327 977
AgeI A'CCGG_T
Cuts at: 5816 5816
Size: 6985
AhdI GACnn_n'nnGTC
Cuts at: 2587 5714 2587
Size: 3127 3858
AleI CACnn'nnGTG
Cuts at: 1166 3809 1166
Size: 2643 4342
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3580 4090 1184
Size: 2396 510 4079
Fragments arranged by size:
4079 2396 510
AlwNI CAG_nnn'CTG
Cuts at: 2548 2681 3700 4728 6300 2548
Size: 133 1019 1028 1572 3233
Fragments arranged by size:
3233 1572 1028 1019 133
ApaI G_GGCC'C
Cuts at: 2081 2132 2172 2183 2662 3503 2081
Size: 51 40 11 479 841 5563
Fragments arranged by size:
5563 841 479 51 40 11
ApaLI G'TGCA_C
Cuts at: 3013 4040 5042 6198 3013
Size: 1027 1002 1156 3800
Fragments arranged by size:
3800 1156 1027 1002
ApoI r'AATT_y
Cuts at: 1493 4294 4520 4672 6781 1493
Size: 2801 226 152 2109 1697
Fragments arranged by size:
2801 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5539 160
Size: 872 4507 1606
Fragments arranged by size:
4507 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6985
AvaI C'yCGr_G
Cuts at: 1870 1943 2120 2165 3918 4310 1870
Size: 73 177 45 1753 392 4545
Fragments arranged by size:
4545 1753 392 177 73 45
BamHI G'GATC_C
Cuts at: 2205 3601 4315 4682 2205
Size: 1396 714 367 4508
Fragments arranged by size:
4508 1396 714 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2902 3722 4306 5761 618
Size: 325 217 627 1115 820 584 1455 1842
Fragments arranged by size:
1842 1455 1115 820 627 584 325 217
BanII G_rGCy'C
Cuts at: 1391 2081 2132 2172 2183 2190 2631 2662 2671
Size: 690 51 40 11 7 441 31 9
Cuts at: 2671 2691 2975 2998 3032 3493 3503 4304 1391
Size: 20 284 23 34 461 10 801 4072
Fragments arranged by size:
4072 801 690 461 441 284 51 40
34 31 23 20 11 10 9 7
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2832 2922 2937 3175 3308 4270 928
Size: 971 933 90 15 238 133 962 3643
Fragments arranged by size:
3643 971 962 933 238 133 90 15
BbvCI CC'TCA_GC
Cuts at: 3263 3593 3263
Size: 330 6655
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5176 5210 1903
Size: 34 3239 34 3678
Fragments arranged by size:
3678 3239 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4877 6087 4877
Size: 1210 5775
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6985
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2666 5596 136
Size: 107 122 71 631 605 994 2930 1525
Fragments arranged by size:
2930 1525 994 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3235 6980 3235
Size: 3745 3240
BlpI GC'TnA_GC
Cuts at: 3632 4402 3632
Size: 770 6215
Bme1580I G_kGCm'C
Cuts at: 1790 2081 2132 2172 2183 2662 2907 3017 3503
Size: 291 51 40 11 479 245 110 486
Cuts at: 3503 3901 4044 5046 6202 1790
Size: 398 143 1002 1156 2573
Fragments arranged by size:
2573 1156 1002 486 479 398 291 245
143 110 51 40 11
BmgBI CAC'GTC
Cuts at: 1318 3306 3640 1318
Size: 1988 334 4663
Fragments arranged by size:
4663 1988 334
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2100 3497 4716
Size: 416 53 83 399 24 683 1397 1219
Cuts at: 4716 5674 442
Size: 958 1753
Fragments arranged by size:
1753 1397 1219 958 683 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6985
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2516 2645 2982 3665 3932 4162 5645 1239
Size: 1277 129 337 683 267 230 1483 2579
Fragments arranged by size:
2579 1483 1277 683 337 267 230 129
Bpu10I CC'TnA_GC
Cuts at: 2068 2192 2289 2974 3263 3593 2068
Size: 124 97 685 289 330 5460
Fragments arranged by size:
5460 685 330 289 124 97
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3395 5110 5975 6273 3395
Size: 1715 865 298 4107
Fragments arranged by size:
4107 1715 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2110 5648 882
Size: 617 611 3538 2219
Fragments arranged by size:
3538 2219 617 611
BsaAI yAC'GTr
Cuts at: 493 1204 4776 493
Size: 711 3572 2702
Fragments arranged by size:
3572 2702 711
BsaBI GATnn'nnATC
Cuts at: 6979 6979
Size: 6985
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3180 3660 4792 5174
Size: 53 83 186 936 1647 480 1132 382
Cuts at: 5174 5809 275
Size: 635 1451
Fragments arranged by size:
1647 1451 1132 936 635 480 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5418 5816 6090 6237 6648 5418
Size: 398 274 147 411 5755
Fragments arranged by size:
5755 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6760
Fragments arranged by size:
6760 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2027 2038 2741 3163 3893 4214 1186
Size: 841 11 703 422 730 321 3957
Fragments arranged by size:
3957 841 730 703 422 321 11
BseYI C'CCAG_C
Cuts at: 2259 2611 2788 3138 3693 3743 4229 6188 6684
Size: 352 177 350 555 50 486 1959 496
Cuts at: 6684 2259
Size: 2560
Fragments arranged by size:
2560 1959 555 496 486 352 350 177
50
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2195 2879 3754 4187 1163
Size: 573 459 684 875 433 3961
Fragments arranged by size:
3961 875 684 573 459 433
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2025 2034 4435 5196 6224 1540
Size: 275 186 24 9 2401 761 1028 2301
Fragments arranged by size:
2401 2301 1028 761 275 186 24 9
BsiHKAI G_wGCw'C
Cuts at: 2631 3017 3795 4044 5046 5131 6202 2631
Size: 386 778 249 1002 85 1071 3414
Fragments arranged by size:
3414 1071 1002 778 386 249 85
BsmI GAATG_Cn'
Cuts at: 3069 3345 3457 4100 4496 4589 3069
Size: 276 112 643 396 93 5465
Fragments arranged by size:
5465 643 396 276 112 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4142 4142
Size: 6985
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2081 2132 2172 2183 2190 2631 2662
Size: 399 291 51 40 11 7 441 31
Cuts at: 2662 2671 2691 2907 2975 2998 3017 3032 3493
Size: 9 20 216 68 23 19 15 461
Cuts at: 3493 3503 3795 3901 4044 4304 5046 5131 6202
Size: 10 292 106 143 260 742 85 1071
Cuts at: 6202 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 461 441 399 292 291
260 216 143 106 85 68 51 40
31 23 20 19 15 11 10 9
7
BspEI T'CCGG_A
Cuts at: 6648 6648
Size: 6985
BspHI T'CATG_A
Cuts at: 4874 6604 4874
Size: 1730 5255
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2126 3090 3432 4082 4340 844
Size: 1282 964 342 650 258 3489
Fragments arranged by size:
3489 1282 964 650 342 258
BsrBI CCG'CTC
Cuts at: 1394 1823 2021 2062 2619 3858 4872 1394
Size: 429 198 41 557 1239 1014 3507
Fragments arranged by size:
3507 1239 1014 557 429 198 41
BsrDI GCAATG_nn'
Cuts at: 65 1248 4242 5480 5654 65
Size: 1183 2994 1238 174 1396
Fragments arranged by size:
2994 1396 1238 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4036 5629 5816 1700
Size: 110 2226 1593 187 2869
Fragments arranged by size:
2869 2226 1593 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6985
BssHII G'CGCG_C
Cuts at: 1925 2234 2272 1925
Size: 309 38 6638
Fragments arranged by size:
6638 309 38
BssSI C'ACGA_G
Cuts at: 2225 4196 5045 6057 2225
Size: 1971 849 1012 3153
Fragments arranged by size:
3153 1971 1012 849
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6985
BstBI TT'CG_AA
Cuts at: 2241 5789 2241
Size: 3548 3437
BstEII G'GTnAC_C
Cuts at: 1184 2488 1184
Size: 1304 5681
BstXI CCAn_nnnn'nTGG
Cuts at: 2899 3811 2899
Size: 912 6073
BstYI r'GATC_y
Cuts at: 1972 2205 2796 3235 3601 4315 4682 5067 5084
Size: 233 591 439 366 714 367 385 17
Cuts at: 5084 6536 6622 6634 6980 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 714 591 439 385 367 366
346 233 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2028 2172 2478 3652 4047 4083 513
Size: 552 963 144 306 1174 395 36 3415
Fragments arranged by size:
3415 1174 963 552 395 306 144 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6246
Fragments arranged by size:
6246 683 56
BtsI GCAGTG_nn'
Cuts at: 2646 3901 4501 5295 5315 2646
Size: 1255 600 794 20 4316
Fragments arranged by size:
4316 1255 794 600 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6985
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6950
DraI TTT'AAA
Cuts at: 4289 4289
Size: 6985
DraIII CAC_nnn'GTG
Cuts at: 3844 3844
Size: 6985
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5992 809
Size: 507 4676 1802
Fragments arranged by size:
4676 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2022 3146 3441
Size: 54 1425 50 275 18 192 1124 295
Cuts at: 3441 4269 4432 5321 6950 8
Size: 828 163 889 1629 43
Fragments arranged by size:
1629 1425 1124 889 828 295 275 192
163 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2022 4432 1537
Size: 275 210 2410 4090
Fragments arranged by size:
4090 2410 275 210
EarI CTCTTCn'nnn_
Cuts at: 1353 2547 4915 1353
Size: 1194 2368 3423
Fragments arranged by size:
3423 2368 1194
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2098 2169 2368 5572 5946 6092
Size: 1064 138 509 71 199 3204 374 146
Cuts at: 6092 387
Size: 1280
Fragments arranged by size:
3204 1280 1064 509 374 199 146 138
71
EcoICRI GAG'CTC
Cuts at: 2629 2629
Size: 6985
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2288 2399 2429 2516 2645 2982 3664
Size: 393 656 111 30 87 129 337 682
Cuts at: 3664 3665 3932 4162 5048 5645 6432 6700 1239
Size: 1 267 230 886 597 787 268 1524
Fragments arranged by size:
1524 886 787 682 656 597 393 337
268 267 230 129 111 87 30 1
EcoNI CCTnn'n_nnAGG
Cuts at: 2429 2552 3428 4271 2429
Size: 123 876 843 5143
Fragments arranged by size:
5143 876 843 123
EcoO109I rG'GnC_Cy
Cuts at: 2078 2658 2659 2678 3499 3670 3739 3799 4237
Size: 580 1 19 821 171 69 60 438
Cuts at: 4237 2078
Size: 4826
Fragments arranged by size:
4826 821 580 438 171 69 60 19
1
EcoRI G'AATT_C
Cuts at: 4294 4672 4294
Size: 378 6607
FspI TGC'GCA
Cuts at: 5491 5491
Size: 6985
HaeII r_GCGC'y
Cuts at: 1678 2773 3461 3700 4260 6132 1678
Size: 1095 688 239 560 1872 2531
Fragments arranged by size:
2531 1872 1095 688 560 239
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2573 2605 5626 5658 5700
Size: 32 135 32 1001 32 3021 32 42
Cuts at: 5700 5732 1373
Size: 32 2626
Fragments arranged by size:
3021 2626 1001 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4329 4575 669
Size: 988 281 2391 246 3079
Fragments arranged by size:
3079 2391 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6985
HpaI GTT'AAC
Cuts at: 4575 4575
Size: 6985
KpnI G_GTAC'C
Cuts at: 4310 4310
Size: 6985
MfeI C'AATT_G
Cuts at: 4584 4584
Size: 6985
MluI A'CGCG_T
Cuts at: 6861 6861
Size: 6985
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2563 3557 3959 6099 6283 1305
Size: 1258 994 402 2140 184 2007
Fragments arranged by size:
2140 2007 1258 994 402 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4271 10
Size: 54 1425 343 2439 2724
Fragments arranged by size:
2724 2439 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3809 4943 5302 5461 518
Size: 648 2643 1134 359 159 2042
Fragments arranged by size:
2643 2042 1134 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2030 2259 3267 3930 4401 5078 6226 6471
Size: 424 229 1008 663 471 677 1148 245
Cuts at: 6471 1606
Size: 2120
Fragments arranged by size:
2120 1148 1008 677 663 471 424 245
229
NcoI C'CATG_G
Cuts at: 513 1065 4047 513
Size: 552 2982 3451
Fragments arranged by size:
3451 2982 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6985
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6985
NotI GC'GGCC_GC
Cuts at: 2022 4432 2022
Size: 2410 4575
NspI r_CATG'y
Cuts at: 1132 1229 2587 3482 4343 5888 1132
Size: 97 1358 895 861 1545 2229
Fragments arranged by size:
2229 1545 1358 895 861 97
PasI CC'CwG_GG
Cuts at: 1348 2664 1348
Size: 1316 5669
PciI A'CATG_T
Cuts at: 5884 5884
Size: 6985
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3040 3643 3700 3844 1108
Size: 1932 603 57 144 4249
Fragments arranged by size:
4249 1932 603 144 57
PfoI T'CCnGG_A
Cuts at: 3642 4014 3642
Size: 372 6613
PmeI GTTT'AAAC
Cuts at: 4289 4289
Size: 6985
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5054 5086 6567 6599 5054
Size: 32 1481 32 5440
Fragments arranged by size:
5440 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3739 3799 3739
Size: 60 6925
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6985
PsiI TTA'TAA
Cuts at: 4555 4555
Size: 6985
PspOMI G'GGCC_C
Cuts at: 2077 2128 2168 2179 2658 3499 2077
Size: 51 40 11 479 841 5563
Fragments arranged by size:
5563 841 479 51 40 11
PstI C_TGCA'G
Cuts at: 830 2686 2811 3429 4337 830
Size: 1856 125 618 908 3478
Fragments arranged by size:
3478 1856 908 618 125
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6985
PvuII CAG'CTG
Cuts at: 3267 3267
Size: 6985
SacI G_AGCT'C
Cuts at: 2631 2631
Size: 6985
SacII CC_GC'GG
Cuts at: 2031 2031
Size: 6985
SalI G'TCGA_C
Cuts at: 1936 4327 1936
Size: 2391 4594
SanDI GG'GwC_CC
Cuts at: 3739 3799 3739
Size: 60 6925
SbfI CC_TGCA'GG
Cuts at: 2686 4337 2686
Size: 1651 5334
ScaI AGT'ACT
Cuts at: 5233 6945 5233
Size: 1712 5273
SexAI A'CCwGG_T
Cuts at: 2343 2343
Size: 6985
SfcI C'TryA_G
Cuts at: 826 1045 2682 2807 3425 3544 4333 5468 6149
Size: 219 1637 125 618 119 789 1135 681
Cuts at: 6149 6340 826
Size: 191 1471
Fragments arranged by size:
1637 1471 1135 789 681 618 219 191
125 119
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6985
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6985
SmaI CCC'GGG
Cuts at: 1872 2122 2167 3920 4312 1872
Size: 250 45 1753 392 4545
Fragments arranged by size:
4545 1753 392 250 45
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3410 5089 5990 6252 820
Size: 197 926 1467 1679 901 262 1553
Fragments arranged by size:
1679 1553 1467 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4776 493
Size: 4283 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6985
SphI G_CATG'C
Cuts at: 1132 4343 1132
Size: 3211 3774
SspI AAT'ATT
Cuts at: 5 52 4909 5
Size: 47 4857 2081
Fragments arranged by size:
4857 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6985
StyI C'CwwG_G
Cuts at: 513 1065 2892 3064 4047 4272 4424 513
Size: 552 1827 172 983 225 152 3074
Fragments arranged by size:
3074 1827 983 552 225 172 152
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2476 5012 5029 5182 5367 5812 325
Size: 1255 896 2536 17 153 185 445 1498
Fragments arranged by size:
2536 1498 1255 896 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5231 6943 96
Size: 274 80 33 51 1443 3254 1712 138
Fragments arranged by size:
3254 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3650 3682 6945 6977 3650
Size: 32 3263 32 3658
Fragments arranged by size:
3658 3263 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3869 4067 1316
Size: 2553 198 4234
Fragments arranged by size:
4234 2553 198
XbaI T'CTAG_A
Cuts at: 4321 4321
Size: 6985
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2476 2476
Size: 6985
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6985
XmaI C'CCGG_G
Cuts at: 1870 2120 2165 3918 4310 1870
Size: 250 45 1753 392 4545
Fragments arranged by size:
4545 1753 392 250 45
XmnI GAAnn'nnTTC
Cuts at: 5114 5114
Size: 6985
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3181 4793 276
Size: 53 83 186 936 1647 1612 2468
Fragments arranged by size:
2468 1647 1612 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SacII SalI SanDI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI
BstZ17I Bsu36I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI
RsrII SapI SfiI SfoI SrfI SwaI