Restriction map for
pFN21AB3513
(Circular) (Six-base) MAPSORT of: pFN21AB3513.seq Check: 7686 from: 1 to: 7774
pFN21AB3513 7774 bp
With 182 enzymes: SgfI *
February 27, 2009 14:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4763 4763
Size: 7774
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5584 278
Size: 53 83 186 936 4048 2468
Fragments arranged by size:
4048 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2781 5117 1937
Size: 844 2336 4594
Fragments arranged by size:
4594 2336 844
Acc65I G'GTAC_C
Cuts at: 5095 5095
Size: 7774
AclI AA'CG_TT
Cuts at: 1563 5901 6274 1563
Size: 4338 373 3063
Fragments arranged by size:
4338 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2129 3231 3723 5837 7221 7489 1632
Size: 497 1102 492 2114 1384 268 1917
Fragments arranged by size:
2114 1917 1384 1102 497 492 268
AflII C'TTAA_G
Cuts at: 820 1017 3332 820
Size: 197 2315 5262
Fragments arranged by size:
5262 2315 197
AflIII A'CryG_T
Cuts at: 1203 3174 6673 7650 1203
Size: 1971 3499 977 1327
Fragments arranged by size:
3499 1971 1327 977
AgeI A'CCGG_T
Cuts at: 6605 6605
Size: 7774
AhdI GACnn_n'nnGTC
Cuts at: 6503 6503
Size: 7774
AleI CACnn'nnGTG
Cuts at: 1166 2894 4005 1166
Size: 1728 1111 4935
Fragments arranged by size:
4935 1728 1111
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2650 1184
Size: 1466 6308
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2862 2894 2862
Size: 32 7742
AlwNI CAG_nnn'CTG
Cuts at: 3592 4591 5517 7089 3592
Size: 999 926 1572 4277
Fragments arranged by size:
4277 1572 999 926
ApaLI G'TGCA_C
Cuts at: 5831 6987 5831
Size: 1156 6618
ApoI r'AATT_y
Cuts at: 1493 2598 3733 4828 5083 5309 5461 7570 1493
Size: 1105 1135 1095 255 226 152 2109 1697
Fragments arranged by size:
2109 1697 1135 1105 1095 255 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6328 160
Size: 872 5296 1606
Fragments arranged by size:
5296 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7774
AvaI C'yCGr_G
Cuts at: 1870 1943 4358 5099 1870
Size: 73 2415 741 4545
Fragments arranged by size:
4545 2415 741 73
AvrII C'CTAG_G
Cuts at: 3661 3661
Size: 7774
BamHI G'GATC_C
Cuts at: 3543 5104 5471 3543
Size: 1561 367 5846
Fragments arranged by size:
5846 1561 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2759 3530 5095 6550 618
Size: 325 217 627 972 771 1565 1455 1842
Fragments arranged by size:
1842 1565 1455 972 771 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3507 3768 5093 1391
Size: 2116 261 1325 4072
Fragments arranged by size:
4072 2116 1325 261
BbeI G_GCGC'C
Cuts at: 3534 3534
Size: 7774
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3936 4707 4828 928
Size: 971 2037 771 121 3874
Fragments arranged by size:
3874 2037 971 771 121
BbvCI CC'TCA_GC
Cuts at: 2502 4089 2502
Size: 1587 6187
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5965 5999 1903
Size: 34 4028 34 3678
Fragments arranged by size:
4028 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4956 5666 6876 4956
Size: 710 1210 5854
Fragments arranged by size:
5854 1210 710
BclI T'GATC_A
Cuts at: 1548 2131 4551 1548
Size: 583 2420 4771
Fragments arranged by size:
4771 2420 583
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5046 6385 136
Size: 107 122 71 631 605 3374 1339 1525
Fragments arranged by size:
3374 1525 1339 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4860 7769 4860
Size: 2909 4865
BlpI GC'TnA_GC
Cuts at: 5191 5191
Size: 7774
Bme1580I G_kGCm'C
Cuts at: 1790 4445 5835 6991 1790
Size: 2655 1390 1156 2573
Fragments arranged by size:
2655 2573 1390 1156
BmgBI CAC'GTC
Cuts at: 1318 2024 3114 1318
Size: 706 1090 5978
Fragments arranged by size:
5978 1090 706
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2796 3950 4418
Size: 416 53 83 399 24 1379 1154 468
Cuts at: 4418 4681 5505 6463 442
Size: 263 824 958 1753
Fragments arranged by size:
1753 1379 1154 958 824 468 416 399
263 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7774
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2673 2735 4155 4305 6434 1239
Size: 1434 62 1420 150 2129 2579
Fragments arranged by size:
2579 2129 1434 1420 150 62
Bpu10I CC'TnA_GC
Cuts at: 2502 4089 2502
Size: 1587 6187
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2200 2963 2978 5899 6764 7062 2200
Size: 763 15 2921 865 298 2912
Fragments arranged by size:
2921 2912 865 763 298 15
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2586 2691 3092 3852 3914 6437 882
Size: 617 1087 105 401 760 62 2523 2219
Fragments arranged by size:
2523 2219 1087 760 617 401 105 62
BsaAI yAC'GTr
Cuts at: 493 1204 2768 5565 493
Size: 711 1564 2797 2702
Fragments arranged by size:
2797 2702 1564 711
BsaBI GATnn'nnATC
Cuts at: 2194 7768 2194
Size: 5574 2200
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3531 3612 5038 5581
Size: 53 83 186 936 1998 81 1426 543
Cuts at: 5581 5963 6598 275
Size: 382 635 1451
Fragments arranged by size:
1998 1451 1426 936 635 543 382 186
83 81 53
BsaWI w'CCGG_w
Cuts at: 3156 6207 6605 6879 7026 7437 3156
Size: 3051 398 274 147 411 3493
Fragments arranged by size:
3493 3051 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2236 2266 2671 2701 3642
Size: 30 165 30 833 30 405 30 941
Cuts at: 3642 3672 4010 4040 1178
Size: 30 338 30 4912
Fragments arranged by size:
4912 941 833 405 338 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2604 2641 3560 3614 1186
Size: 1418 37 919 54 5346
Fragments arranged by size:
5346 1418 919 54 37
BseYI C'CCAG_C
Cuts at: 3637 5049 6977 7473 3637
Size: 1412 1928 496 3938
Fragments arranged by size:
3938 1928 1412 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2226 4811 1163
Size: 573 490 2585 4126
Fragments arranged by size:
4126 2585 573 490
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3798 5224 5985 7013 1540
Size: 275 186 1797 1426 761 1028 2301
Fragments arranged by size:
2301 1797 1426 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5835 5920 6991 5835
Size: 85 1071 6618
Fragments arranged by size:
6618 1071 85
BsmI GAATG_Cn'
Cuts at: 5285 5378 5285
Size: 93 7681
BsmBI CGTCTCn'nnnn_
Cuts at: 2711 2711
Size: 7774
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3507 3768 4445 5093 5835 5920 6991
Size: 399 1717 261 677 648 742 85 1071
Cuts at: 6991 1391
Size: 2174
Fragments arranged by size:
2174 1717 1071 742 677 648 399 261
85
BspEI T'CCGG_A
Cuts at: 7437 7437
Size: 7774
BspHI T'CATG_A
Cuts at: 4497 5663 7393 4497
Size: 1166 1730 4878
Fragments arranged by size:
4878 1730 1166
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3269 4763 5129 844
Size: 2425 1494 366 3489
Fragments arranged by size:
3489 2425 1494 366
BsrBI CCG'CTC
Cuts at: 1394 1823 4546 5661 1394
Size: 429 2723 1115 3507
Fragments arranged by size:
3507 2723 1115 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2727 2853 3922 3933 4329 6269 6443
Size: 1183 1479 126 1069 11 396 1940 174
Cuts at: 6443 65
Size: 1396
Fragments arranged by size:
1940 1479 1396 1183 1069 396 174 126
11
BsrFI r'CCGG_y
Cuts at: 1700 1810 6418 6605 1700
Size: 110 4608 187 2869
Fragments arranged by size:
4608 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7774
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7774
BssSI C'ACGA_G
Cuts at: 2202 5834 6846 2202
Size: 3632 1012 3130
Fragments arranged by size:
3632 3130 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3353 794
Size: 2559 5215
BstBI TT'CG_AA
Cuts at: 2521 6578 2521
Size: 4057 3717
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7774
BstXI CCAn_nnnn'nTGG
Cuts at: 3055 3895 4468 3055
Size: 840 573 6361
Fragments arranged by size:
6361 840 573
BstYI r'GATC_y
Cuts at: 1972 2214 2569 3543 4860 5104 5471 5856 5873
Size: 242 355 974 1317 244 367 385 17
Cuts at: 5873 7325 7411 7423 7769 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1317 974 385 367 355 346
244 242 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3574 3788 3810 3924 513
Size: 552 939 1570 214 22 114 4363
Fragments arranged by size:
4363 1570 939 552 214 114 22
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7035
Fragments arranged by size:
7035 683 56
BtsI GCAGTG_nn'
Cuts at: 3303 4012 4724 4799 5290 6084 6104 3303
Size: 709 712 75 491 794 20 4973
Fragments arranged by size:
4973 794 712 709 491 75 20
ClaI AT'CG_AT
Cuts at: 1554 3744 1554
Size: 2190 5584
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7739
DraI TTT'AAA
Cuts at: 5078 5078
Size: 7774
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3106 6781 809
Size: 507 1790 3675 1802
Fragments arranged by size:
3675 1802 1790 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3015 3795 5221
Size: 54 1425 50 275 18 1185 780 1426
Cuts at: 5221 6110 7739 8
Size: 889 1629 43
Fragments arranged by size:
1629 1426 1425 1185 889 780 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3795 5221 1537
Size: 275 1983 1426 4090
Fragments arranged by size:
4090 1983 1426 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2364 4496 4878 4995 5704 1353
Size: 1011 2132 382 117 709 3423
Fragments arranged by size:
3423 2132 1011 709 382 117
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6361 6735 6881 387
Size: 1064 138 4772 374 146 1280
Fragments arranged by size:
4772 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2129 2673 2735 3231 3723 4155 4305
Size: 393 497 544 62 496 492 432 150
Cuts at: 4305 5837 6434 7221 7489 1239
Size: 1532 597 787 268 1524
Fragments arranged by size:
1532 1524 787 597 544 497 496 492
432 393 268 150 62
EcoNI CCTnn'n_nnAGG
Cuts at: 3596 3596
Size: 7774
EcoO109I rG'GnC_Cy
Cuts at: 3643 4080 4588 3643
Size: 437 508 6829
Fragments arranged by size:
6829 508 437
EcoRI G'AATT_C
Cuts at: 2598 4828 5083 5461 2598
Size: 2230 255 378 4911
Fragments arranged by size:
4911 2230 378 255
FspI TGC'GCA
Cuts at: 6280 6280
Size: 7774
HaeII r_GCGC'y
Cuts at: 1678 3534 6921 1678
Size: 1856 3387 2531
Fragments arranged by size:
3387 2531 1856
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2636 2668 2671 2703 3202
Size: 32 135 32 1064 32 3 32 499
Cuts at: 3202 3234 3642 3674 3769 3801 4010 4042 4072
Size: 32 408 32 95 32 209 32 30
Cuts at: 4072 4104 6415 6447 6489 6521 1373
Size: 32 2311 32 42 32 2626
Fragments arranged by size:
2626 2311 1064 499 408 209 135 95
42 32 32 32 32 32 32 32
32 32 32 32 30 3
HincII GTy'rAC
Cuts at: 669 1657 1938 5118 5364 669
Size: 988 281 3180 246 3079
Fragments arranged by size:
3180 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7774
HpaI GTT'AAC
Cuts at: 5364 5364
Size: 7774
KasI G'GCGC_C
Cuts at: 3530 3530
Size: 7774
KpnI G_GTAC'C
Cuts at: 5099 5099
Size: 7774
MfeI C'AATT_G
Cuts at: 2746 4275 5373 2746
Size: 1529 1098 5147
Fragments arranged by size:
5147 1529 1098
MluI A'CGCG_T
Cuts at: 7650 7650
Size: 7774
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1999 6888 7072 1305
Size: 694 4889 184 2007
Fragments arranged by size:
4889 2007 694 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3017 10
Size: 54 1425 343 1185 4767
Fragments arranged by size:
4767 1425 1185 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2801 2894 3773 4005 4106 4448 5732
Size: 648 1635 93 879 232 101 342 1284
Cuts at: 5732 6091 6250 518
Size: 359 159 2042
Fragments arranged by size:
2042 1635 1284 879 648 359 342 232
159 101 93
MspA1I CmG'CkG
Cuts at: 1606 2045 2210 4036 4683 5190 5867 7015 7260
Size: 439 165 1826 647 507 677 1148 245
Cuts at: 7260 1606
Size: 2120
Fragments arranged by size:
2120 1826 1148 677 647 507 439 245
165
NarI GG'CG_CC
Cuts at: 3531 3531
Size: 7774
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6283
Fragments arranged by size:
6283 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7774
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7774
NotI GC'GGCC_GC
Cuts at: 5221 5221
Size: 7774
NspI r_CATG'y
Cuts at: 1132 1229 3178 5132 6677 1132
Size: 97 1949 1954 1545 2229
Fragments arranged by size:
2229 1954 1949 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3975 4591 1348
Size: 2627 616 4531
Fragments arranged by size:
4531 2627 616
PciI A'CATG_T
Cuts at: 3174 6673 3174
Size: 3499 4275
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4591 1108
Size: 3483 4291
PmeI GTTT'AAAC
Cuts at: 5078 5078
Size: 7774
PmlI CAC'GTG
Cuts at: 2768 2768
Size: 7774
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3133 3165 5843 5875 7356 7388 3133
Size: 32 2678 32 1481 32 3519
Fragments arranged by size:
3519 2678 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4080 4080
Size: 7774
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7774
PsiI TTA'TAA
Cuts at: 5344 5344
Size: 7774
PstI C_TGCA'G
Cuts at: 830 2044 2863 5126 830
Size: 1214 819 2263 3478
Fragments arranged by size:
3478 2263 1214 819
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7774
PvuII CAG'CTG
Cuts at: 2045 2210 4036 2045
Size: 165 1826 5783
Fragments arranged by size:
5783 1826 165
SalI G'TCGA_C
Cuts at: 1936 5116 1936
Size: 3180 4594
SbfI CC_TGCA'GG
Cuts at: 5126 5126
Size: 7774
ScaI AGT'ACT
Cuts at: 6022 7734 6022
Size: 1712 6062
SexAI A'CCwGG_T
Cuts at: 4961 4961
Size: 7774
SfcI C'TryA_G
Cuts at: 826 1045 2040 2364 2859 5122 6257 6938 7129
Size: 219 995 324 495 2263 1135 681 191
Cuts at: 7129 826
Size: 1471
Fragments arranged by size:
2263 1471 1135 995 681 495 324 219
191
SfoI GGC'GCC
Cuts at: 3532 3532
Size: 7774
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7774
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7774
SmaI CCC'GGG
Cuts at: 1872 5101 1872
Size: 3229 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2179 2942 2957 3332 5878 6779
Size: 197 926 236 763 15 375 2546 901
Cuts at: 6779 7041 820
Size: 262 1553
Fragments arranged by size:
2546 1553 926 901 763 375 262 236
197 15
SnaBI TAC'GTA
Cuts at: 493 5565 493
Size: 5072 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7774
SphI G_CATG'C
Cuts at: 1132 5132 1132
Size: 4000 3774
SspI AAT'ATT
Cuts at: 5 52 5698 5
Size: 47 5646 2081
Fragments arranged by size:
5646 2081 47
StuI AGG'CCT
Cuts at: 1517 3989 1517
Size: 2472 5302
StyI C'CwwG_G
Cuts at: 513 1065 2004 3102 3661 3984 5213 513
Size: 552 939 1098 559 323 1229 3074
Fragments arranged by size:
3074 1229 1098 939 559 552 323
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2390 2610 2900 4822 4916 5041 5801
Size: 1255 810 220 290 1922 94 125 760
Cuts at: 5801 5818 5971 6156 6601 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1922 1498 1255 810 760 445 290 220
185 153 125 94 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2946 3997 6020
Size: 274 80 33 51 1443 969 1051 2023
Cuts at: 6020 7732 96
Size: 1712 138
Fragments arranged by size:
2023 1712 1443 1051 969 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2236 2268 7734 7766 2236
Size: 32 5466 32 2244
Fragments arranged by size:
5466 2244 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2683 4805 5012 1316
Size: 1367 2122 207 4078
Fragments arranged by size:
4078 2122 1367 207
XbaI T'CTAG_A
Cuts at: 5110 5110
Size: 7774
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7774
XmaI C'CCGG_G
Cuts at: 1870 5099 1870
Size: 3229 4545
XmnI GAAnn'nnTTC
Cuts at: 3696 4634 5903 3696
Size: 938 1269 5567
Fragments arranged by size:
5567 1269 938
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5582 276
Size: 53 83 186 936 4048 2468
Fragments arranged by size:
4048 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI BaeI BfrBI BplI BsiWI BstZ17I
Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NaeI
NgoMIV NruI NsiI PacI PfoI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SfiI SrfI SwaI
XcmI