Restriction map for
pFN21AB3509
(Circular) (Six-base) MAPSORT of: pFN21AB3509.seq Check: 8502 from: 1 to: 5803
pFN21AB3509 5803 bp
With 182 enzymes: SgfI *
October 20, 2009 10:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3613 278
Size: 53 83 186 936 2077 2468
Fragments arranged by size:
2468 2077 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3146 1937
Size: 1209 4594
Acc65I G'GTAC_C
Cuts at: 3124 3124
Size: 5803
AclI AA'CG_TT
Cuts at: 1563 3930 4303 1563
Size: 2367 373 3063
Fragments arranged by size:
3063 2367 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2232 3866 5250 5518 1632
Size: 600 1634 1384 268 1917
Fragments arranged by size:
1917 1634 1384 600 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5606
AflIII A'CryG_T
Cuts at: 1203 4702 5679 1203
Size: 3499 977 1327
Fragments arranged by size:
3499 1327 977
AgeI A'CCGG_T
Cuts at: 4634 4634
Size: 5803
AhdI GACnn_n'nnGTC
Cuts at: 2384 4532 2384
Size: 2148 3655
AleI CACnn'nnGTG
Cuts at: 1166 2594 1166
Size: 1428 4375
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5803
AlwNI CAG_nnn'CTG
Cuts at: 3546 5118 3546
Size: 1572 4231
ApaI G_GGCC'C
Cuts at: 2016 2016
Size: 5803
ApaLI G'TGCA_C
Cuts at: 2597 3860 5016 2597
Size: 1263 1156 3384
Fragments arranged by size:
3384 1263 1156
ApoI r'AATT_y
Cuts at: 1493 2139 2335 2681 2761 3112 3338 3490 5599
Size: 646 196 346 80 351 226 152 2109
Cuts at: 5599 1493
Size: 1697
Fragments arranged by size:
2109 1697 646 351 346 226 196 152
80
AseI AT'TA_AT
Cuts at: 160 1032 4357 160
Size: 872 3325 1606
Fragments arranged by size:
3325 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5803
AvaI C'yCGr_G
Cuts at: 1870 1943 3128 1870
Size: 73 1185 4545
Fragments arranged by size:
4545 1185 73
BamHI G'GATC_C
Cuts at: 3133 3500 3133
Size: 367 5436
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3124 4579 618
Size: 325 217 627 1337 1455 1842
Fragments arranged by size:
1842 1455 1337 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2016 3122 1391
Size: 625 1106 4072
Fragments arranged by size:
4072 1106 625
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2309 928
Size: 971 410 4422
Fragments arranged by size:
4422 971 410
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3994 4028 1903
Size: 34 2057 34 3678
Fragments arranged by size:
3678 2057 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3695 4905 3695
Size: 1210 4593
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5803
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4414 136
Size: 107 122 71 631 605 2742 1525
Fragments arranged by size:
2742 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5798 5798
Size: 5803
BlpI GC'TnA_GC
Cuts at: 3220 3220
Size: 5803
Bme1580I G_kGCm'C
Cuts at: 1790 2016 2601 3864 5020 1790
Size: 226 585 1263 1156 2573
Fragments arranged by size:
2573 1263 1156 585 226
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5803
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3534 4492 442
Size: 416 53 83 399 24 2117 958 1753
Fragments arranged by size:
2117 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5803
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2247 2868 4463 1239
Size: 1008 621 1595 2579
Fragments arranged by size:
2579 1595 1008 621
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3928 4793 5091 3928
Size: 865 298 4640
Fragments arranged by size:
4640 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2038 2816 4466 882
Size: 617 539 778 1650 2219
Fragments arranged by size:
2219 1650 778 617 539
BsaAI yAC'GTr
Cuts at: 493 1204 3594 493
Size: 711 2390 2702
Fragments arranged by size:
2702 2390 711
BsaBI GATnn'nnATC
Cuts at: 5797 5797
Size: 5803
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3610 3992 4627 275
Size: 53 83 186 936 2077 382 635 1451
Fragments arranged by size:
2077 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2577 4236 4634 4908 5055 5466 2577
Size: 1659 398 274 147 411 2914
Fragments arranged by size:
2914 1659 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5578
Fragments arranged by size:
5578 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2210 1186
Size: 1024 4779
BseYI C'CCAG_C
Cuts at: 2009 2044 5006 5502 2009
Size: 35 2962 496 2310
Fragments arranged by size:
2962 2310 496 35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2519 2814 1163
Size: 573 783 295 4152
Fragments arranged by size:
4152 783 573 295
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3253 4014 5042 1540
Size: 275 186 1252 761 1028 2301
Fragments arranged by size:
2301 1252 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2601 3864 3949 5020 2601
Size: 1263 85 1071 3384
Fragments arranged by size:
3384 1263 1071 85
BsmI GAATG_Cn'
Cuts at: 2051 3314 3407 2051
Size: 1263 93 4447
Fragments arranged by size:
4447 1263 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2016 2601 3122 3864 3949 5020 1391
Size: 399 226 585 521 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 585 521 399 226 85
BspEI T'CCGG_A
Cuts at: 5466 5466
Size: 5803
BspHI T'CATG_A
Cuts at: 3692 5422 3692
Size: 1730 4073
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3158 844
Size: 2314 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2200 3690 1394
Size: 429 377 1490 3507
Fragments arranged by size:
3507 1490 429 377
BsrDI GCAATG_nn'
Cuts at: 65 1248 2538 2889 3052 4298 4472 65
Size: 1183 1290 351 163 1246 174 1396
Fragments arranged by size:
1396 1290 1246 1183 351 174 163
BsrFI r'CCGG_y
Cuts at: 1700 1810 2318 4447 4634 1700
Size: 110 508 2129 187 2869
Fragments arranged by size:
2869 2129 508 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5803
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5803
BssSI C'ACGA_G
Cuts at: 3863 4875 3863
Size: 1012 4791
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5803
BstBI TT'CG_AA
Cuts at: 4607 4607
Size: 5803
BstEII G'GTnAC_C
Cuts at: 1184 2071 2321 1184
Size: 887 250 4666
Fragments arranged by size:
4666 887 250
BstYI r'GATC_y
Cuts at: 1972 2022 2728 3133 3500 3885 3902 5354 5440
Size: 50 706 405 367 385 17 1452 86
Cuts at: 5440 5452 5798 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 706 405 385 367 346 86
50 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2265 513
Size: 552 939 261 4051
Fragments arranged by size:
4051 939 552 261
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5064
Fragments arranged by size:
5064 683 56
BtsI GCAGTG_nn'
Cuts at: 2208 3319 4113 4133 2208
Size: 1111 794 20 3878
Fragments arranged by size:
3878 1111 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5803
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5768
DraI TTT'AAA
Cuts at: 3107 3107
Size: 5803
DraIII CAC_nnn'GTG
Cuts at: 2828 2828
Size: 5803
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4810 809
Size: 507 3494 1802
Fragments arranged by size:
3494 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3250 4139 5768
Size: 54 1425 50 275 18 1420 889 1629
Cuts at: 5768 8
Size: 43
Fragments arranged by size:
1629 1425 1420 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3250 1537
Size: 275 1438 4090
Fragments arranged by size:
4090 1438 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3733 1353
Size: 2380 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4390 4764 4910 387
Size: 1064 138 2801 374 146 1280
Fragments arranged by size:
2801 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2232 2247 2868 3866 4463 5250 5518
Size: 393 600 15 621 998 597 787 268
Cuts at: 5518 1239
Size: 1524
Fragments arranged by size:
1524 998 787 621 600 597 393 268
15
EcoO109I rG'GnC_Cy
Cuts at: 2222 2294 2222
Size: 72 5731
EcoRI G'AATT_C
Cuts at: 3112 3490 3112
Size: 378 5425
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2302 2302
Size: 5803
FspI TGC'GCA
Cuts at: 2348 4309 2348
Size: 1961 3842
HaeII r_GCGC'y
Cuts at: 1678 4950 1678
Size: 3272 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2370 2402 2794 2826 4444
Size: 32 135 32 798 32 392 32 1618
Cuts at: 4444 4476 4518 4550 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1618 798 392 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3147 3393 669
Size: 988 281 1209 246 3079
Fragments arranged by size:
3079 1209 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5803
HpaI GTT'AAC
Cuts at: 3393 3393
Size: 5803
KpnI G_GTAC'C
Cuts at: 3128 3128
Size: 5803
MfeI C'AATT_G
Cuts at: 3402 3402
Size: 5803
MluI A'CGCG_T
Cuts at: 5679 5679
Size: 5803
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2401 4917 5101 1305
Size: 1096 2516 184 2007
Fragments arranged by size:
2516 2007 1096 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3981
Fragments arranged by size:
3981 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2594 2618 3761 4120 4279 518
Size: 648 1428 24 1143 359 159 2042
Fragments arranged by size:
2042 1428 1143 648 359 159 24
MspA1I CmG'CkG
Cuts at: 1606 2147 3219 3896 5044 5289 1606
Size: 541 1072 677 1148 245 2120
Fragments arranged by size:
2120 1148 1072 677 541 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2265 513
Size: 552 939 261 4051
Fragments arranged by size:
4051 939 552 261
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5803
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5803
NotI GC'GGCC_GC
Cuts at: 3250 3250
Size: 5803
NspI r_CATG'y
Cuts at: 1132 1229 2480 3161 4706 1132
Size: 97 1251 681 1545 2229
Fragments arranged by size:
2229 1545 1251 681 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5803
PciI A'CATG_T
Cuts at: 4702 4702
Size: 5803
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2123 1108
Size: 1015 4788
PfoI T'CCnGG_A
Cuts at: 2116 2116
Size: 5803
PmeI GTTT'AAAC
Cuts at: 3107 3107
Size: 5803
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3872 3904 5385 5417 3872
Size: 32 1481 32 4258
Fragments arranged by size:
4258 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2222 2222
Size: 5803
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5803
PsiI TTA'TAA
Cuts at: 3373 3373
Size: 5803
PspOMI G'GGCC_C
Cuts at: 2012 2012
Size: 5803
PstI C_TGCA'G
Cuts at: 830 2152 3155 830
Size: 1322 1003 3478
Fragments arranged by size:
3478 1322 1003
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5803
PvuII CAG'CTG
Cuts at: 2147 2147
Size: 5803
SalI G'TCGA_C
Cuts at: 1936 3145 1936
Size: 1209 4594
SbfI CC_TGCA'GG
Cuts at: 3155 3155
Size: 5803
ScaI AGT'ACT
Cuts at: 4051 5763 4051
Size: 1712 4091
SfcI C'TryA_G
Cuts at: 826 1045 2148 3151 4286 4967 5158 826
Size: 219 1103 1003 1135 681 191 1471
Fragments arranged by size:
1471 1135 1103 1003 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5803
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5803
SmaI CCC'GGG
Cuts at: 1872 3130 1872
Size: 1258 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3907 4808 5070 820
Size: 197 926 1964 901 262 1553
Fragments arranged by size:
1964 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3594 493
Size: 3101 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5803
SphI G_CATG'C
Cuts at: 1132 3161 1132
Size: 2029 3774
SspI AAT'ATT
Cuts at: 5 52 2428 3727 5
Size: 47 2376 1299 2081
Fragments arranged by size:
2376 2081 1299 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5803
StyI C'CwwG_G
Cuts at: 513 1065 2004 2265 3242 513
Size: 552 939 261 977 3074
Fragments arranged by size:
3074 977 939 552 261
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2340 3830 3847 4000 4185 4630 325
Size: 1255 760 1490 17 153 185 445 1498
Fragments arranged by size:
1498 1490 1255 760 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4049 5761 96
Size: 274 80 33 51 1443 2072 1712 138
Fragments arranged by size:
2072 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5763 5795 5763
Size: 32 5771
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5803
XbaI T'CTAG_A
Cuts at: 3139 3139
Size: 5803
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5803
XmaI C'CCGG_G
Cuts at: 1870 3128 1870
Size: 1258 4545
XmnI GAAnn'nnTTC
Cuts at: 3932 3932
Size: 5803
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3611 276
Size: 53 83 186 936 2077 2468
Fragments arranged by size:
2468 2077 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI