Restriction map for
pFN21AB3508
(Circular) (Six-base) MAPSORT of: pFN21AB3508.seq Check: 1566 from: 1 to: 5689
pFN21AB3508 5689 bp
With 182 enzymes: SgfI *
October 20, 2009 10:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3499 278
Size: 53 83 186 936 1963 2468
Fragments arranged by size:
2468 1963 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3032 1937
Size: 1095 4594
Acc65I G'GTAC_C
Cuts at: 3010 3010
Size: 5689
AclI AA'CG_TT
Cuts at: 1563 3816 4189 1563
Size: 2253 373 3063
Fragments arranged by size:
3063 2253 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2845 2896 2942 3752 5136 5404 1632
Size: 1213 51 46 810 1384 268 1917
Fragments arranged by size:
1917 1384 1213 810 268 51 46
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5492
AflIII A'CryG_T
Cuts at: 1203 2314 4588 5565 1203
Size: 1111 2274 977 1327
Fragments arranged by size:
2274 1327 1111 977
AgeI A'CCGG_T
Cuts at: 4520 4520
Size: 5689
AhdI GACnn_n'nnGTC
Cuts at: 4418 4418
Size: 5689
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5689
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5689
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2118 2150 2118
Size: 32 5657
AlwNI CAG_nnn'CTG
Cuts at: 2519 3432 5004 2519
Size: 913 1572 3204
Fragments arranged by size:
3204 1572 913
ApaI G_GGCC'C
Cuts at: 2367 2367
Size: 5689
ApaLI G'TGCA_C
Cuts at: 3746 4902 3746
Size: 1156 4533
ApoI r'AATT_y
Cuts at: 1493 2074 2998 3224 3376 5485 1493
Size: 581 924 226 152 2109 1697
Fragments arranged by size:
2109 1697 924 581 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4243 160
Size: 872 3211 1606
Fragments arranged by size:
3211 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5689
AvaI C'yCGr_G
Cuts at: 1870 1943 3014 1870
Size: 73 1071 4545
Fragments arranged by size:
4545 1071 73
BamHI G'GATC_C
Cuts at: 3019 3386 3019
Size: 367 5322
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3010 4465 618
Size: 325 217 627 1223 1455 1842
Fragments arranged by size:
1842 1455 1223 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2367 2950 3008 1391
Size: 976 583 58 4072
Fragments arranged by size:
4072 976 583 58
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2317 928
Size: 971 418 4300
Fragments arranged by size:
4300 971 418
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3880 3914 1903
Size: 34 1943 34 3678
Fragments arranged by size:
3678 1943 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2329 3581 4791 2329
Size: 1252 1210 3227
Fragments arranged by size:
3227 1252 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5689
BfrBI ATG'CAT
Cuts at: 2645 2797 2645
Size: 152 5537
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4300 136
Size: 107 122 71 631 605 2628 1525
Fragments arranged by size:
2628 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2835 5684 2835
Size: 2849 2840
BlpI GC'TnA_GC
Cuts at: 2813 3106 2813
Size: 293 5396
Bme1580I G_kGCm'C
Cuts at: 1790 2367 3750 4906 1790
Size: 577 1383 1156 2573
Fragments arranged by size:
2573 1383 1156 577
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5689
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3420 4378 442
Size: 416 53 83 399 24 2003 958 1753
Fragments arranged by size:
2003 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5689
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2379 2408 4349 1239
Size: 1140 29 1941 2579
Fragments arranged by size:
2579 1941 1140 29
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2969 3814 4679 4977 2969
Size: 845 865 298 3681
Fragments arranged by size:
3681 865 845 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2213 2681 4352 882
Size: 617 714 468 1671 2219
Fragments arranged by size:
2219 1671 714 617 468
BsaAI yAC'GTr
Cuts at: 493 1204 2317 3480 493
Size: 711 1113 1163 2702
Fragments arranged by size:
2702 1163 1113 711
BsaBI GATnn'nnATC
Cuts at: 2723 5683 2723
Size: 2960 2729
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3496 3878 4513 275
Size: 53 83 186 936 1963 382 635 1451
Fragments arranged by size:
1963 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4122 4520 4794 4941 5352 4122
Size: 398 274 147 411 4459
Fragments arranged by size:
4459 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5464
Fragments arranged by size:
5464 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2405 2432 2972 1186
Size: 1219 27 540 3903
Fragments arranged by size:
3903 1219 540 27
BseYI C'CCAG_C
Cuts at: 4892 5388 4892
Size: 496 5193
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2492 1163
Size: 573 756 4360
Fragments arranged by size:
4360 756 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3139 3900 4928 1540
Size: 275 186 1138 761 1028 2301
Fragments arranged by size:
2301 1138 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2343 2439 3750 3835 4906 2343
Size: 96 1311 85 1071 3126
Fragments arranged by size:
3126 1311 1071 96 85
BsmI GAATG_Cn'
Cuts at: 2604 2797 3200 3293 2604
Size: 193 403 93 5000
Fragments arranged by size:
5000 403 193 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2343 2367 2439 2950 3008 3750 3835
Size: 399 553 24 72 511 58 742 85
Cuts at: 3835 4906 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 553 511 399 85 72
58 24
BspEI T'CCGG_A
Cuts at: 5352 5352
Size: 5689
BspHI T'CATG_A
Cuts at: 3578 5308 3578
Size: 1730 3959
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3044 844
Size: 2200 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3576 1394
Size: 429 1753 3507
Fragments arranged by size:
3507 1753 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4184 4358 65
Size: 1183 2936 174 1396
Fragments arranged by size:
2936 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2382 4333 4520 1700
Size: 110 572 1951 187 2869
Fragments arranged by size:
2869 1951 572 187 110
BsrGI T'GTAC_A
Cuts at: 96 2298 96
Size: 2202 3487
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5689
BssSI C'ACGA_G
Cuts at: 3749 4761 3749
Size: 1012 4677
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2803 794
Size: 2009 3680
BstBI TT'CG_AA
Cuts at: 4493 4493
Size: 5689
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5689
BstYI r'GATC_y
Cuts at: 1972 2394 2835 3019 3386 3771 3788 5240 5326
Size: 422 441 184 367 385 17 1452 86
Cuts at: 5326 5338 5684 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 441 422 385 367 346 184
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4198
Fragments arranged by size:
4198 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4950
Fragments arranged by size:
4950 683 56
BtsI GCAGTG_nn'
Cuts at: 3205 3999 4019 3205
Size: 794 20 4875
Fragments arranged by size:
4875 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5689
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5654
DraI TTT'AAA
Cuts at: 2993 2993
Size: 5689
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4696 809
Size: 507 3380 1802
Fragments arranged by size:
3380 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3136 4025 5654
Size: 54 1425 50 275 18 1306 889 1629
Cuts at: 5654 8
Size: 43
Fragments arranged by size:
1629 1425 1306 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3136 1537
Size: 275 1324 4090
Fragments arranged by size:
4090 1324 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2262 3619 1353
Size: 909 1357 3423
Fragments arranged by size:
3423 1357 909
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4276 4650 4796 387
Size: 1064 138 2687 374 146 1280
Fragments arranged by size:
2687 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2379 2408 2845 2896 2942 3752 4349
Size: 393 747 29 437 51 46 810 597
Cuts at: 4349 5136 5404 1239
Size: 787 268 1524
Fragments arranged by size:
1524 810 787 747 597 437 393 268
51 46 29
EcoNI CCTnn'n_nnAGG
Cuts at: 2333 2333
Size: 5689
EcoO109I rG'GnC_Cy
Cuts at: 2363 2363
Size: 5689
EcoRI G'AATT_C
Cuts at: 2074 2998 3376 2074
Size: 924 378 4387
Fragments arranged by size:
4387 924 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2359 2359
Size: 5689
FspI TGC'GCA
Cuts at: 4195 4195
Size: 5689
HaeII r_GCGC'y
Cuts at: 1678 4836 1678
Size: 3158 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2601 2633 4330 4362 4404
Size: 32 135 32 1029 32 1697 32 42
Cuts at: 4404 4436 1373
Size: 32 2626
Fragments arranged by size:
2626 1697 1029 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2212 2350 3033 3279 669
Size: 988 281 274 138 683 246 3079
Fragments arranged by size:
3079 988 683 281 274 246 138
HindIII A'AGCT_T
Cuts at: 748 2128 2954 748
Size: 1380 826 3483
Fragments arranged by size:
3483 1380 826
HpaI GTT'AAC
Cuts at: 3279 3279
Size: 5689
KpnI G_GTAC'C
Cuts at: 3014 3014
Size: 5689
MfeI C'AATT_G
Cuts at: 3288 3288
Size: 5689
MluI A'CGCG_T
Cuts at: 5565 5565
Size: 5689
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2412 2422 2732 4803 4987 1305
Size: 1107 10 310 2071 184 2007
Fragments arranged by size:
2071 2007 1107 310 184 10
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3867
Fragments arranged by size:
3867 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3647 4006 4165 518
Size: 648 2481 359 159 2042
Fragments arranged by size:
2481 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2889 3105 3782 4930 5175 1606
Size: 1283 216 677 1148 245 2120
Fragments arranged by size:
2120 1283 1148 677 245 216
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4198
Fragments arranged by size:
4198 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5689
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5689
NotI GC'GGCC_GC
Cuts at: 3136 3136
Size: 5689
NsiI A_TGCA'T
Cuts at: 2647 2799 2647
Size: 152 5537
NspI r_CATG'y
Cuts at: 1132 1229 3047 4592 1132
Size: 97 1818 1545 2229
Fragments arranged by size:
2229 1818 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5689
PciI A'CATG_T
Cuts at: 4588 4588
Size: 5689
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2386 1108
Size: 1278 4411
PmeI GTTT'AAAC
Cuts at: 2993 2993
Size: 5689
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3758 3790 5271 5303 3758
Size: 32 1481 32 4144
Fragments arranged by size:
4144 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5689
PsiI TTA'TAA
Cuts at: 2561 3259 2561
Size: 698 4991
PspOMI G'GGCC_C
Cuts at: 2363 2363
Size: 5689
PstI C_TGCA'G
Cuts at: 830 3041 830
Size: 2211 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5689
SalI G'TCGA_C
Cuts at: 1936 3031 1936
Size: 1095 4594
SbfI CC_TGCA'GG
Cuts at: 3041 3041
Size: 5689
ScaI AGT'ACT
Cuts at: 3937 5649 3937
Size: 1712 3977
SfcI C'TryA_G
Cuts at: 826 1045 2654 3037 4172 4853 5044 826
Size: 219 1609 383 1135 681 191 1471
Fragments arranged by size:
1609 1471 1135 681 383 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5689
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5689
SmaI CCC'GGG
Cuts at: 1872 3016 1872
Size: 1144 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2984 3793 4694 4956 820
Size: 197 926 1041 809 901 262 1553
Fragments arranged by size:
1553 1041 926 901 809 262 197
SnaBI TAC'GTA
Cuts at: 493 3480 493
Size: 2987 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5689
SphI G_CATG'C
Cuts at: 1132 3047 1132
Size: 1915 3774
SspI AAT'ATT
Cuts at: 5 52 3613 5
Size: 47 3561 2081
Fragments arranged by size:
3561 2081 47
StuI AGG'CCT
Cuts at: 1517 2456 2866 1517
Size: 939 410 4340
Fragments arranged by size:
4340 939 410
StyI C'CwwG_G
Cuts at: 513 1065 2004 2425 3128 513
Size: 552 939 421 703 3074
Fragments arranged by size:
3074 939 703 552 421
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3716 3733 3886 4071 4516 325
Size: 1255 2136 17 153 185 445 1498
Fragments arranged by size:
2136 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2298 3935 5647
Size: 274 80 33 51 1443 321 1637 1712
Cuts at: 5647 96
Size: 138
Fragments arranged by size:
1712 1637 1443 321 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2070 2102 2272 2304 5649 5681 2070
Size: 32 170 32 3345 32 2078
Fragments arranged by size:
3345 2078 170 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5689
XbaI T'CTAG_A
Cuts at: 3025 3025
Size: 5689
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5689
XmaI C'CCGG_G
Cuts at: 1870 3014 1870
Size: 1144 4545
XmnI GAAnn'nnTTC
Cuts at: 3818 3818
Size: 5689
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3497 276
Size: 53 83 186 936 1963 2468
Fragments arranged by size:
2468 1963 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI