Restriction map for
pFN21AB3499
(Circular) (Six-base) MAPSORT of: pFN21AB3499.seq Check: 8964 from: 1 to: 7921
pFN21AB3499 7921 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3222 3612 3683 3836 3222
Size: 390 71 153 7307
Fragments arranged by size:
7307 390 153 71
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2021 5731 278
Size: 53 83 186 936 485 3710 2468
Fragments arranged by size:
3710 2468 936 485 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2367 2439 2832 5264 1937
Size: 430 72 393 2432 4594
Fragments arranged by size:
4594 2432 430 393 72
Acc65I G'GTAC_C
Cuts at: 2914 5242 2914
Size: 2328 5593
AclI AA'CG_TT
Cuts at: 1563 6048 6421 1563
Size: 4485 373 3063
Fragments arranged by size:
4485 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4361 4916 5984 7368 7636 1632
Size: 2729 555 1068 1384 268 1917
Fragments arranged by size:
2729 1917 1384 1068 555 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7724
AflIII A'CryG_T
Cuts at: 1203 3186 4360 6820 7797 1203
Size: 1983 1174 2460 977 1327
Fragments arranged by size:
2460 1983 1327 1174 977
AgeI A'CCGG_T
Cuts at: 6752 6752
Size: 7921
AhdI GACnn_n'nnGTC
Cuts at: 4321 6650 4321
Size: 2329 5592
AleI CACnn'nnGTG
Cuts at: 1166 2879 4265 1166
Size: 1713 1386 4822
Fragments arranged by size:
4822 1713 1386
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2168 2258 3059 1184
Size: 984 90 801 6046
Fragments arranged by size:
6046 984 801 90
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4100 4132 4100
Size: 32 7889
AlwNI CAG_nnn'CTG
Cuts at: 3926 4597 5664 7236 3926
Size: 671 1067 1572 4611
Fragments arranged by size:
4611 1572 1067 671
ApaI G_GGCC'C
Cuts at: 2689 2980 3155 4540 2689
Size: 291 175 1385 6070
Fragments arranged by size:
6070 1385 291 175
ApaLI G'TGCA_C
Cuts at: 2731 2738 5978 7134 2731
Size: 7 3240 1156 3518
Fragments arranged by size:
3518 3240 1156 7
ApoI r'AATT_y
Cuts at: 1493 4157 4205 5196 5230 5456 5608 7717 1493
Size: 2664 48 991 34 226 152 2109 1697
Fragments arranged by size:
2664 2109 1697 991 226 152 48 34
AseI AT'TA_AT
Cuts at: 160 1032 6475 160
Size: 872 5443 1606
Fragments arranged by size:
5443 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7921
AvaI C'yCGr_G
Cuts at: 1870 1943 2098 2490 2963 3388 3801 4300 4692
Size: 73 155 392 473 425 413 499 392
Cuts at: 4692 5151 5246 1870
Size: 459 95 4545
Fragments arranged by size:
4545 499 473 459 425 413 392 392
155 95 73
AvrII C'CTAG_G
Cuts at: 4843 4843
Size: 7921
BamHI G'GATC_C
Cuts at: 3393 3883 4144 5251 5618 3393
Size: 490 261 1107 367 5696
Fragments arranged by size:
5696 1107 490 367 261
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2024 2191 2552 2770 2809
Size: 325 217 627 237 167 361 218 39
Cuts at: 2809 2914 3341 3824 4076 5242 6697 618
Size: 105 427 483 252 1166 1455 1842
Fragments arranged by size:
1842 1455 1166 627 483 427 361 325
252 237 218 217 167 105 39
BanII G_rGCy'C
Cuts at: 1391 2499 2689 2980 3155 3253 3611 3801 3870
Size: 1108 190 291 175 98 358 190 69
Cuts at: 3870 4540 5158 5240 1391
Size: 670 618 82 4072
Fragments arranged by size:
4072 1108 670 618 358 291 190 190
175 98 82 69
BbeI G_GCGC'C
Cuts at: 2813 2813
Size: 7921
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2371 2890 3879 4904 928
Size: 971 472 519 989 1025 3945
Fragments arranged by size:
3945 1025 989 971 519 472
BbvCI CC'TCA_GC
Cuts at: 3373 4015 4653 3373
Size: 642 638 6641
Fragments arranged by size:
6641 642 638
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3898 3932 4291 4325 6112 6146 1903
Size: 34 1961 34 359 34 1787 34 3678
Fragments arranged by size:
3678 1961 1787 359 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5813 7023 5813
Size: 1210 6711
BclI T'GATC_A
Cuts at: 1548 4777 1548
Size: 3229 4692
BfrBI ATG'CAT
Cuts at: 5207 5207
Size: 7921
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3539 3705 3765
Size: 107 122 71 631 605 1867 166 60
Cuts at: 3765 4395 6532 136
Size: 630 2137 1525
Fragments arranged by size:
2137 1867 1525 631 630 605 166 122
107 71 60
BglII A'GATC_T
Cuts at: 4575 7916 4575
Size: 3341 4580
BlpI GC'TnA_GC
Cuts at: 5338 5338
Size: 7921
Bme1580I G_kGCm'C
Cuts at: 1790 2196 2434 2597 2689 2735 2742 2775 2980
Size: 406 238 163 92 46 7 33 205
Cuts at: 2980 3155 4081 4540 5982 7138 1790
Size: 175 926 459 1442 1156 2573
Fragments arranged by size:
2573 1442 1156 926 459 406 238 205
175 163 92 46 33 7
BmgBI CAC'GTC
Cuts at: 1318 2911 1318
Size: 1593 6328
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2122 3213 5652
Size: 416 53 83 399 24 705 1091 2439
Cuts at: 5652 6610 442
Size: 958 1753
Fragments arranged by size:
2439 1753 1091 958 705 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7921
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2821 3403 4312 2821
Size: 582 909 6430
Fragments arranged by size:
6430 909 582
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2645 2687 3602 4160 4492 5039 6581 1239
Size: 1406 42 915 558 332 547 1542 2579
Fragments arranged by size:
2579 1542 1406 915 558 547 332 42
Bpu10I CC'TnA_GC
Cuts at: 3373 4015 4653 4875 3373
Size: 642 638 222 6419
Fragments arranged by size:
6419 642 638 222
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2357 2468 6046 6911 7209 2357
Size: 111 3578 865 298 3069
Fragments arranged by size:
3578 3069 865 298 111
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3008 3719 4120 4308 6584 882
Size: 617 1509 711 401 188 2276 2219
Fragments arranged by size:
2276 2219 1509 711 617 401 188
BsaAI yAC'GTr
Cuts at: 493 1204 2229 4720 5712 493
Size: 711 1025 2491 992 2702
Fragments arranged by size:
2702 2491 1025 992 711
BsaBI GATnn'nnATC
Cuts at: 2264 7915 2264
Size: 5651 2270
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2018 2178 2810 2829
Size: 53 83 186 936 485 160 632 19
Cuts at: 2829 3165 5063 5728 6110 6745 275
Size: 336 1898 665 382 635 1451
Fragments arranged by size:
1898 1451 936 665 635 632 485 382
336 186 160 83 53 19
BsaWI w'CCGG_w
Cuts at: 2021 2298 3081 4032 4073 4908 6354 6752 7026
Size: 277 783 951 41 835 1446 398 274
Cuts at: 7026 7173 7584 2021
Size: 147 411 2358
Fragments arranged by size:
2358 1446 951 835 783 411 398 277
274 147 41
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2108 2138 2300 2330 3150
Size: 30 165 30 705 30 162 30 820
Cuts at: 3150 3180 3218 3248 3664 3694 3839 3869 4100
Size: 30 38 30 416 30 145 30 231
Cuts at: 4100 4130 4496 4526 4720 4750 1178
Size: 30 366 30 194 30 4349
Fragments arranged by size:
4349 820 705 416 366 231 194 165
162 145 38 30 30 30 30 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2201 2276 2771 3551 3605 3860 3863 4003
Size: 1015 75 495 780 54 255 3 140
Cuts at: 4003 4057 4394 4397 4741 4952 5053 5123 5126
Size: 54 337 3 344 211 101 70 3
Cuts at: 5126 1186
Size: 3981
Fragments arranged by size:
3981 1015 780 495 344 337 255 211
140 101 75 70 54 54 3 3
3
BseYI C'CCAG_C
Cuts at: 2195 2682 2979 3498 3683 7124 7620 2195
Size: 487 297 519 185 3441 496 2496
Fragments arranged by size:
3441 2496 519 496 487 297 185
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2603 3194 3582 1163
Size: 573 867 591 388 5502
Fragments arranged by size:
5502 867 591 573 388
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3054 5371 6132 7160 1540
Size: 275 186 1053 2317 761 1028 2301
Fragments arranged by size:
2317 2301 1053 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2499 2678 2735 2742 5982 6067 7138 2499
Size: 179 57 7 3240 85 1071 3282
Fragments arranged by size:
3282 3240 1071 179 85 57 7
BsmI GAATG_Cn'
Cuts at: 2529 3331 5432 5525 2529
Size: 802 2101 93 4925
Fragments arranged by size:
4925 2101 802 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3568 3952 3568
Size: 384 7537
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2196 2434 2499 2597 2678 2689 2735
Size: 399 406 238 65 98 81 11 46
Cuts at: 2735 2742 2775 2980 3155 3253 3611 3801 3870
Size: 7 33 205 175 98 358 190 69
Cuts at: 3870 4081 4540 5158 5240 5982 6067 7138 1391
Size: 211 459 618 82 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 618 459 406 399 358
238 211 205 190 175 98 98 85
82 81 69 65 46 33 11 7
BspEI T'CCGG_A
Cuts at: 4032 7584 4032
Size: 3552 4369
BspHI T'CATG_A
Cuts at: 5810 7540 5810
Size: 1730 6191
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2926 3222 3612 3683 3704 3836 4326 4336
Size: 2082 296 390 71 21 132 490 10
Cuts at: 4336 5276 844
Size: 940 3489
Fragments arranged by size:
3489 2082 940 490 390 296 132 71
21 10
BsrBI CCG'CTC
Cuts at: 1394 1823 3161 4402 4534 4879 5808 1394
Size: 429 1338 1241 132 345 929 3507
Fragments arranged by size:
3507 1338 1241 929 429 345 132
BsrDI GCAATG_nn'
Cuts at: 65 1248 4967 6416 6590 65
Size: 1183 3719 1449 174 1396
Fragments arranged by size:
3719 1449 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2141 2279 2744 3199 4083 4901 4990
Size: 110 331 138 465 455 884 818 89
Cuts at: 4990 6565 6752 1700
Size: 1575 187 2869
Fragments arranged by size:
2869 1575 884 818 465 455 331 187
138 110 89
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7921
BssHII G'CGCG_C
Cuts at: 1925 2748 2750 1925
Size: 823 2 7096
Fragments arranged by size:
7096 823 2
BssSI C'ACGA_G
Cuts at: 3785 5981 6993 3785
Size: 2196 1012 4713
Fragments arranged by size:
4713 2196 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3978 794
Size: 3184 4737
BstBI TT'CG_AA
Cuts at: 6725 6725
Size: 7921
BstEII G'GTnAC_C
Cuts at: 1184 2782 3367 1184
Size: 1598 585 5738
Fragments arranged by size:
5738 1598 585
BstXI CCAn_nnnn'nTGG
Cuts at: 2848 3118 4516 4752 2848
Size: 270 1398 236 6017
Fragments arranged by size:
6017 1398 270 236
BstYI r'GATC_y
Cuts at: 1972 2259 3393 3883 4134 4144 4575 5251 5618
Size: 287 1134 490 251 10 431 676 367
Cuts at: 5618 6003 6020 7472 7558 7570 7916 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1134 676 490 431 385 367
346 287 251 86 17 12 10
BstZ17I GTA'TAC
Cuts at: 2440 2440
Size: 7921
Bsu36I CC'TnA_GG
Cuts at: 2184 3750 3843 2184
Size: 1566 93 6262
Fragments arranged by size:
6262 1566 93
BtgI C'CryG_G
Cuts at: 513 1065 2004 2028 2344 2823 2847 2877 4101
Size: 552 939 24 316 479 24 30 1224
Cuts at: 4101 4119 4263 4545 513
Size: 18 144 282 3889
Fragments arranged by size:
3889 1224 939 552 479 316 282 144
30 24 24 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3729 487
Size: 683 56 2503 4679
Fragments arranged by size:
4679 2503 683 56
BtsI GCAGTG_nn'
Cuts at: 3709 3976 4495 5437 6231 6251 3709
Size: 267 519 942 794 20 5379
Fragments arranged by size:
5379 942 794 519 267 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7921
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5155 5190 529
Size: 35 4591 35 3260
Fragments arranged by size:
4591 3260 35 35
DraI TTT'AAA
Cuts at: 5225 5225
Size: 7921
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6928 809
Size: 507 5612 1802
Fragments arranged by size:
5612 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2420 2724 2883
Size: 54 1425 50 275 18 590 304 159
Cuts at: 2883 3462 3531 3706 4269 4599 4903 5368 6257
Size: 579 69 175 563 330 304 465 889
Cuts at: 6257 7886 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 590 579 563 465 330
304 304 275 175 159 69 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5368 1537
Size: 275 3556 4090
Fragments arranged by size:
4090 3556 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3938 4075 4701 4718 4889 5851 1353
Size: 2585 137 626 17 171 962 3423
Fragments arranged by size:
3423 2585 962 626 171 137 17
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2192 2558 4675 6508 6882 7028
Size: 1064 138 603 366 2117 1833 374 146
Cuts at: 7028 387
Size: 1280
Fragments arranged by size:
2117 1833 1280 1064 603 374 366 146
138
EcoICRI GAG'CTC
Cuts at: 2497 2497
Size: 7921
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2645 2687 3602 4160 4361 4492 4916
Size: 393 1013 42 915 558 201 131 424
Cuts at: 4916 5039 5984 6581 7368 7636 1239
Size: 123 945 597 787 268 1524
Fragments arranged by size:
1524 1013 945 915 787 597 558 424
393 268 201 131 123 42
EcoNI CCTnn'n_nnAGG
Cuts at: 2402 2922 4033 4343 5105 2402
Size: 520 1111 310 762 5218
Fragments arranged by size:
5218 1111 762 520 310
EcoO109I rG'GnC_Cy
Cuts at: 2517 2686 3151 3418 4380 4472 4537 2517
Size: 169 465 267 962 92 65 5901
Fragments arranged by size:
5901 962 465 267 169 92 65
EcoRI G'AATT_C
Cuts at: 4157 5230 5608 4157
Size: 1073 378 6470
Fragments arranged by size:
6470 1073 378
FspI TGC'GCA
Cuts at: 6427 6427
Size: 7921
HaeII r_GCGC'y
Cuts at: 1678 2813 2847 7068 1678
Size: 1135 34 4221 2531
Fragments arranged by size:
4221 2531 1135 34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2300 2332 3150 3182 4307
Size: 32 135 32 728 32 818 32 1125
Cuts at: 4307 4339 4544 4576 4579 4611 4720 4752 4793
Size: 32 205 32 3 32 109 32 41
Cuts at: 4793 4825 5087 5119 6562 6594 6636 6668 1373
Size: 32 262 32 1443 32 42 32 2626
Fragments arranged by size:
2626 1443 1125 818 728 262 205 135
109 42 41 32 32 32 32 32
32 32 32 32 32 32 32 3
HincII GTy'rAC
Cuts at: 669 1657 1938 2833 4351 5265 5511 669
Size: 988 281 895 1518 914 246 3079
Fragments arranged by size:
3079 1518 988 914 895 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7921
HpaI GTT'AAC
Cuts at: 5511 5511
Size: 7921
KasI G'GCGC_C
Cuts at: 2809 2809
Size: 7921
KpnI G_GTAC'C
Cuts at: 2918 5246 2918
Size: 2328 5593
MfeI C'AATT_G
Cuts at: 4761 5520 4761
Size: 759 7162
MluI A'CGCG_T
Cuts at: 7797 7797
Size: 7921
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4718 7035 7219 1305
Size: 3413 2317 184 2007
Fragments arranged by size:
3413 2317 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2422 2885 3464 10
Size: 54 1425 343 590 463 579 4467
Fragments arranged by size:
4467 1425 590 579 463 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2606 2879 3458 3714 4265 4359 4527
Size: 648 1440 273 579 256 551 94 168
Cuts at: 4527 5879 6238 6397 518
Size: 1352 359 159 2042
Fragments arranged by size:
2042 1440 1352 648 579 551 359 273
256 168 159 94
MspA1I CmG'CkG
Cuts at: 1606 2030 2065 2346 2649 2704 2825 3118 3502
Size: 424 35 281 303 55 121 293 384
Cuts at: 3502 3926 4019 5122 5337 6014 7162 7407 1606
Size: 424 93 1103 215 677 1148 245 2120
Fragments arranged by size:
2120 1148 1103 677 424 424 384 303
293 281 245 215 121 93 55 35
NaeI GCC'GGC
Cuts at: 2143 2746 4085 2143
Size: 603 1339 5979
Fragments arranged by size:
5979 1339 603
NarI GG'CG_CC
Cuts at: 2810 2810
Size: 7921
NcoI C'CATG_G
Cuts at: 513 1065 2004 4101 4545 513
Size: 552 939 2097 444 3889
Fragments arranged by size:
3889 2097 939 552 444
NdeI CA'TA_TG
Cuts at: 387 3526 387
Size: 3139 4782
NgoMIV G'CCGG_C
Cuts at: 2141 2744 4083 2141
Size: 603 1339 5979
Fragments arranged by size:
5979 1339 603
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7921
NotI GC'GGCC_GC
Cuts at: 5368 5368
Size: 7921
NsiI A_TGCA'T
Cuts at: 5209 5209
Size: 7921
NspI r_CATG'y
Cuts at: 1132 1229 4364 5279 6824 1132
Size: 97 3135 915 1545 2229
Fragments arranged by size:
3135 2229 1545 915 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7921
PciI A'CATG_T
Cuts at: 4360 6820 4360
Size: 2460 5461
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4912 1108
Size: 3804 4117
PfoI T'CCnGG_A
Cuts at: 2268 2622 3879 4137 2268
Size: 354 1257 258 6052
Fragments arranged by size:
6052 1257 354 258
PmeI GTTT'AAAC
Cuts at: 5225 5225
Size: 7921
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4100 4132 5990 6022 7503 7535 4100
Size: 32 1858 32 1481 32 4486
Fragments arranged by size:
4486 1858 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3020 1613
Size: 1407 6514
PsiI TTA'TAA
Cuts at: 5491 5491
Size: 7921
PspOMI G'GGCC_C
Cuts at: 2685 2976 3151 4536 2685
Size: 291 175 1385 6070
Fragments arranged by size:
6070 1385 291 175
PspXI vC'TCGA_Gb
Cuts at: 5151 5151
Size: 7921
PstI C_TGCA'G
Cuts at: 830 2477 3353 4388 4934 5273 830
Size: 1647 876 1035 546 339 3478
Fragments arranged by size:
3478 1647 1035 876 546 339
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7921
PvuII CAG'CTG
Cuts at: 3118 3502 4019 3118
Size: 384 517 7020
Fragments arranged by size:
7020 517 384
SacI G_AGCT'C
Cuts at: 2499 2499
Size: 7921
SacII CC_GC'GG
Cuts at: 2031 2347 2826 2031
Size: 316 479 7126
Fragments arranged by size:
7126 479 316
SalI G'TCGA_C
Cuts at: 1936 2831 5263 1936
Size: 895 2432 4594
Fragments arranged by size:
4594 2432 895
SapI GCTCTTCn'nnn_
Cuts at: 4889 4889
Size: 7921
SbfI CC_TGCA'GG
Cuts at: 5273 5273
Size: 7921
ScaI AGT'ACT
Cuts at: 6169 7881 6169
Size: 1712 6209
SexAI A'CCwGG_T
Cuts at: 4110 4110
Size: 7921
SfcI C'TryA_G
Cuts at: 826 1045 2473 2614 3064 3349 4384 4930 5269
Size: 219 1428 141 450 285 1035 546 339
Cuts at: 5269 6404 7085 7276 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1428 1135 1035 681 546 450 339
285 219 191 141
SfoI GGC'GCC
Cuts at: 2811 2811
Size: 7921
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7921
SgrAI Cr'CCGG_yG
Cuts at: 1700 2744 1700
Size: 1044 6877
SmaI CCC'GGG
Cuts at: 1872 2492 2965 3803 5248 1872
Size: 620 473 838 1445 4545
Fragments arranged by size:
4545 1445 838 620 473
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2098 2372 2483 5151 6025 6926
Size: 197 926 155 274 111 2668 874 901
Cuts at: 6926 7188 820
Size: 262 1553
Fragments arranged by size:
2668 1553 926 901 874 274 262 197
155 111
SnaBI TAC'GTA
Cuts at: 493 5712 493
Size: 5219 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7921
SphI G_CATG'C
Cuts at: 1132 5279 1132
Size: 4147 3774
SspI AAT'ATT
Cuts at: 5 52 5845 5
Size: 47 5793 2081
Fragments arranged by size:
5793 2081 47
StuI AGG'CCT
Cuts at: 1517 4049 1517
Size: 2532 5389
StyI C'CwwG_G
Cuts at: 513 1065 2004 3414 4101 4545 4843 5360 513
Size: 552 939 1410 687 444 298 517 3074
Fragments arranged by size:
3074 1410 939 687 552 517 444 298
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2404 3010 4021 4371 5948 5965 6118
Size: 1255 824 606 1011 350 1577 17 153
Cuts at: 6118 6303 6748 325
Size: 185 445 1498
Fragments arranged by size:
1577 1498 1255 1011 824 606 445 350
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3321 6167 7879
Size: 274 80 33 51 1443 1344 2846 1712
Cuts at: 7879 96
Size: 138
Fragments arranged by size:
2846 1712 1443 1344 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2449 2481 3265 3297 3664 3696 3839 3871 3875
Size: 32 784 32 367 32 143 32 4
Cuts at: 3875 3907 4136 4168 7881 7913 2449
Size: 32 229 32 3713 32 2457
Fragments arranged by size:
3713 2457 784 367 229 143 32 32
32 32 32 32 32 4
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7921
XbaI T'CTAG_A
Cuts at: 5257 5257
Size: 7921
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2203 2605 2848 3302 4642 2203
Size: 402 243 454 1340 5482
Fragments arranged by size:
5482 1340 454 402 243
XhoI C'TCGA_G
Cuts at: 1943 2098 5151 1943
Size: 155 3053 4713
Fragments arranged by size:
4713 3053 155
XmaI C'CCGG_G
Cuts at: 1870 2490 2963 3801 5246 1870
Size: 620 473 838 1445 4545
Fragments arranged by size:
4545 1445 838 620 473
XmnI GAAnn'nnTTC
Cuts at: 3857 6050 3857
Size: 2193 5728
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2019 5729 276
Size: 53 83 186 936 485 3710 2468
Fragments arranged by size:
3710 2468 936 485 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AscI BaeI BsiWI DraIII EcoRV FalI FseI
FspAI NruI PacI PmlI PpuMI PsrI RsrII SanDI
SfiI SrfI SwaI