Restriction map for
pFN21AB3497
(Circular) (Six-base) MAPSORT of: pFN21AB3497.seq Check: 4334 from: 1 to: 9016
pFN21AB3497 9016 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5371 5371
Size: 9016
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6826 278
Size: 53 83 186 936 5290 2468
Fragments arranged by size:
5290 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3173 6359 1937
Size: 1236 3186 4594
Fragments arranged by size:
4594 3186 1236
Acc65I G'GTAC_C
Cuts at: 2419 6337 2419
Size: 3918 5098
AclI AA'CG_TT
Cuts at: 1563 5143 7143 7516 1563
Size: 3580 2000 373 3063
Fragments arranged by size:
3580 3063 2000 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2148 2649 3222 3515 3711 4005 5106 7079
Size: 516 501 573 293 196 294 1101 1973
Cuts at: 7079 8463 8731 1632
Size: 1384 268 1917
Fragments arranged by size:
1973 1917 1384 1101 573 516 501 294
293 268 196
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8819
AflIII A'CryG_T
Cuts at: 1203 3492 7915 8892 1203
Size: 2289 4423 977 1327
Fragments arranged by size:
4423 2289 1327 977
AgeI A'CCGG_T
Cuts at: 7847 7847
Size: 9016
AhdI GACnn_n'nnGTC
Cuts at: 7745 7745
Size: 9016
AleI CACnn'nnGTG
Cuts at: 1166 3372 5704 1166
Size: 2206 2332 4478
Fragments arranged by size:
4478 2332 2206
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3390 4844 4954 5857 1184
Size: 2206 1454 110 903 4343
Fragments arranged by size:
4343 2206 1454 903 110
AlwNI CAG_nnn'CTG
Cuts at: 4028 6759 8331 4028
Size: 2731 1572 4713
Fragments arranged by size:
4713 2731 1572
ApaI G_GGCC'C
Cuts at: 6184 6184
Size: 9016
ApaLI G'TGCA_C
Cuts at: 7073 8229 7073
Size: 1156 7860
ApoI r'AATT_y
Cuts at: 1493 2349 2373 3342 3554 4319 4346 5510 6140
Size: 856 24 969 212 765 27 1164 630
Cuts at: 6140 6325 6551 6703 8812 1493
Size: 185 226 152 2109 1697
Fragments arranged by size:
2109 1697 1164 969 856 765 630 226
212 185 152 27 24
AseI AT'TA_AT
Cuts at: 160 1032 5537 7570 160
Size: 872 4505 2033 1606
Fragments arranged by size:
4505 2033 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9016
AvaI C'yCGr_G
Cuts at: 1870 1943 2919 3150 6341 1870
Size: 73 976 231 3191 4545
Fragments arranged by size:
4545 3191 976 231 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2975 3008 2975
Size: 33 8983
BamHI G'GATC_C
Cuts at: 6346 6713 6346
Size: 367 8649
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2419 2915 3860 6337 7792
Size: 325 217 627 632 496 945 2477 1455
Cuts at: 7792 618
Size: 1842
Fragments arranged by size:
2477 1842 1455 945 632 627 496 325
217
BanII G_rGCy'C
Cuts at: 1391 5578 6184 6335 1391
Size: 4187 606 151 4072
Fragments arranged by size:
4187 4072 606 151
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2450 2505 3848 928
Size: 971 551 55 1343 6096
Fragments arranged by size:
6096 1343 971 551 55
BbvCI CC'TCA_GC
Cuts at: 3027 4389 3027
Size: 1362 7654
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7207 7241 1903
Size: 34 5270 34 3678
Fragments arranged by size:
5270 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2232 2289 4534 6908 8118 2232
Size: 57 2245 2374 1210 3130
Fragments arranged by size:
3130 2374 2245 1210 57
BclI T'GATC_A
Cuts at: 1548 1548
Size: 9016
BfrBI ATG'CAT
Cuts at: 5962 5962
Size: 9016
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7627 136
Size: 107 122 71 631 605 5955 1525
Fragments arranged by size:
5955 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2197 2624 3272 4935 9011 2197
Size: 427 648 1663 4076 2202
Fragments arranged by size:
4076 2202 1663 648 427
BlpI GC'TnA_GC
Cuts at: 6433 6433
Size: 9016
Bme1580I G_kGCm'C
Cuts at: 1790 2920 4793 5390 6184 7077 8233 1790
Size: 1130 1873 597 794 893 1156 2573
Fragments arranged by size:
2573 1873 1156 1130 893 794 597
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9016
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2744 4254 5041
Size: 416 53 83 399 24 1327 1510 787
Cuts at: 5041 6747 7705 442
Size: 1706 958 1753
Fragments arranged by size:
1753 1706 1510 1327 958 787 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9016
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3337 3337
Size: 9016
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3483 4205 4509 5415 6280 7676 1239
Size: 2244 722 304 906 865 1396 2579
Fragments arranged by size:
2579 2244 1396 906 865 722 304
Bpu10I CC'TnA_GC
Cuts at: 3027 4389 4817 3027
Size: 1362 428 7226
Fragments arranged by size:
7226 1362 428
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2906 3316 5188 7141 8006 8304 2906
Size: 410 1872 1953 865 298 3618
Fragments arranged by size:
3618 1953 1872 865 410 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2520 7679 882
Size: 617 1021 5159 2219
Fragments arranged by size:
5159 2219 1021 617
BsaAI yAC'GTr
Cuts at: 493 1204 6807 493
Size: 711 5603 2702
Fragments arranged by size:
5603 2702 711
BsaBI GATnn'nnATC
Cuts at: 4201 9010 4201
Size: 4809 4207
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6823 7205 7840 275
Size: 53 83 186 936 5290 382 635 1451
Fragments arranged by size:
5290 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 7449 7847 8121 8268 8679 7449
Size: 398 274 147 411 7786
Fragments arranged by size:
7786 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 5988 6018 6077 6107 6291
Size: 30 165 30 4585 30 59 30 184
Cuts at: 6291 6321 1178
Size: 30 3873
Fragments arranged by size:
4585 3873 184 165 59 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2400 3313 1186
Size: 1214 913 6889
Fragments arranged by size:
6889 1214 913
BseYI C'CCAG_C
Cuts at: 2182 2880 4792 5389 5472 8219 8715 2182
Size: 698 1912 597 83 2747 496 2483
Fragments arranged by size:
2747 2483 1912 698 597 496 83
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2328 4073 5279 6133 6141 6180 1163
Size: 573 592 1745 1206 854 8 39 3999
Fragments arranged by size:
3999 1745 1206 854 592 573 39 8
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4509 6466 7227 8255 1540
Size: 275 186 2508 1957 761 1028 2301
Fragments arranged by size:
2508 2301 1957 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2559 3103 7077 7162 8233 2559
Size: 544 3974 85 1071 3342
Fragments arranged by size:
3974 3342 1071 544 85
BsmI GAATG_Cn'
Cuts at: 3541 4817 5375 5558 6527 6620 3541
Size: 1276 558 183 969 93 5937
Fragments arranged by size:
5937 1276 969 558 183 93
BsmBI CGTCTCn'nnnn_
Cuts at: 5923 5923
Size: 9016
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2559 2920 3103 4793 5390 5578 6184
Size: 399 769 361 183 1690 597 188 606
Cuts at: 6184 6335 7077 7162 8233 1391
Size: 151 742 85 1071 2174
Fragments arranged by size:
2174 1690 1071 769 742 606 597 399
361 188 183 151 85
BspEI T'CCGG_A
Cuts at: 8679 8679
Size: 9016
BspHI T'CATG_A
Cuts at: 2971 4327 5020 5667 6169 6905 8635 2971
Size: 1356 693 647 502 736 1730 3352
Fragments arranged by size:
3352 1730 1356 736 693 647 502
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3802 3979 3993 5371 6371 844
Size: 2958 177 14 1378 1000 3489
Fragments arranged by size:
3489 2958 1378 1000 177 14
BsrBI CCG'CTC
Cuts at: 1394 1823 5464 6903 1394
Size: 429 3641 1439 3507
Fragments arranged by size:
3641 3507 1439 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2121 3117 4500 7511 7685 65
Size: 1183 873 996 1383 3011 174 1396
Fragments arranged by size:
3011 1396 1383 1183 996 873 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5639 7660 7847 1700
Size: 110 3829 2021 187 2869
Fragments arranged by size:
3829 2869 2021 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9016
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9016
BssSI C'ACGA_G
Cuts at: 5579 7076 8088 5579
Size: 1497 1012 6507
Fragments arranged by size:
6507 1497 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5909 794
Size: 5115 3901
BstBI TT'CG_AA
Cuts at: 7820 7820
Size: 9016
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9016
BstXI CCAn_nnnn'nTGG
Cuts at: 6258 6258
Size: 9016
BstYI r'GATC_y
Cuts at: 1972 2197 2624 3272 4935 5262 6346 6713 7098
Size: 225 427 648 1663 327 1084 367 385
Cuts at: 7098 7115 8567 8653 8665 9011 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1663 1452 1084 648 427 385 367
346 327 225 86 17 12
BstZ17I GTA'TAC
Cuts at: 3174 3174
Size: 9016
Bsu36I CC'TnA_GG
Cuts at: 3828 5480 3828
Size: 1652 7364
BtgI C'CryG_G
Cuts at: 513 1065 2004 4059 6092 6234 513
Size: 552 939 2055 2033 142 3295
Fragments arranged by size:
3295 2055 2033 939 552 142
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 6120 487
Size: 683 56 4894 3383
Fragments arranged by size:
4894 3383 683 56
BtsI GCAGTG_nn'
Cuts at: 4353 4393 5851 6124 6532 7326 7346 4353
Size: 40 1458 273 408 794 20 6023
Fragments arranged by size:
6023 1458 794 408 273 40 20
ClaI AT'CG_AT
Cuts at: 1554 3561 1554
Size: 2007 7009
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3763 3798 529
Size: 35 3199 35 5747
Fragments arranged by size:
5747 3199 35 35
DraI TTT'AAA
Cuts at: 2535 6320 2535
Size: 3785 5231
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8023 809
Size: 507 6707 1802
Fragments arranged by size:
6707 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2137 4506 4925
Size: 54 1425 50 275 18 307 2369 419
Cuts at: 4925 5864 6463 7352 8981 8
Size: 939 599 889 1629 43
Fragments arranged by size:
2369 1629 1425 939 889 599 419 307
275 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4506 6463 1537
Size: 275 2694 1957 4090
Fragments arranged by size:
4090 2694 1957 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2625 3198 3375 3381 3534 4075 5889 5921
Size: 1272 573 177 6 153 541 1814 32
Cuts at: 5921 6946 1353
Size: 1025 3423
Fragments arranged by size:
3423 1814 1272 1025 573 541 177 153
32 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2068 7603 7977 8123 387
Size: 1064 138 479 5535 374 146 1280
Fragments arranged by size:
5535 1280 1064 479 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2148 2649 3222 3483 3515 3711 4005
Size: 393 516 501 573 261 32 196 294
Cuts at: 4005 4205 4509 5106 5415 6280 7079 7676 8463
Size: 200 304 597 309 865 799 597 787
Cuts at: 8463 8731 1239
Size: 268 1524
Fragments arranged by size:
1524 865 799 787 597 597 573 516
501 393 309 304 294 268 261 200
196 32
EcoO109I rG'GnC_Cy
Cuts at: 4972 5476 6180 6181 4972
Size: 504 704 1 7807
Fragments arranged by size:
7807 704 504 1
EcoRI G'AATT_C
Cuts at: 6140 6325 6703 6140
Size: 185 378 8453
Fragments arranged by size:
8453 378 185
EcoRV GAT'ATC
Cuts at: 4426 4426
Size: 9016
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3647 3647
Size: 9016
FspI TGC'GCA
Cuts at: 7522 7522
Size: 9016
HaeII r_GCGC'y
Cuts at: 1678 8163 1678
Size: 6485 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2624 2656 2690 2722 3197
Size: 32 135 32 1052 32 34 32 475
Cuts at: 3197 3229 4040 4072 5550 5582 5922 5954 5988
Size: 32 811 32 1478 32 340 32 34
Cuts at: 5988 6020 7657 7689 7731 7763 1373
Size: 32 1637 32 42 32 2626
Fragments arranged by size:
2626 1637 1478 1052 811 475 340 135
42 34 34 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2698 5610 5775 5886 6360 6606
Size: 988 281 760 2912 165 111 474 246
Cuts at: 6606 669
Size: 3079
Fragments arranged by size:
3079 2912 988 760 474 281 246 165
111
HindIII A'AGCT_T
Cuts at: 748 5077 748
Size: 4329 4687
HpaI GTT'AAC
Cuts at: 5610 5775 6606 5610
Size: 165 831 8020
Fragments arranged by size:
8020 831 165
KpnI G_GTAC'C
Cuts at: 2423 6341 2423
Size: 3918 5098
MfeI C'AATT_G
Cuts at: 6615 6615
Size: 9016
MluI A'CGCG_T
Cuts at: 8892 8892
Size: 9016
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2801 8130 8314 1305
Size: 1496 5329 184 2007
Fragments arranged by size:
5329 2007 1496 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2139 4927 10
Size: 54 1425 343 307 2788 4099
Fragments arranged by size:
4099 2788 1425 343 307 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3372 3455 4091 4229 4826 5704 6974
Size: 648 2206 83 636 138 597 878 1270
Cuts at: 6974 7333 7492 518
Size: 359 159 2042
Fragments arranged by size:
2206 2042 1270 878 648 636 597 359
159 138 83
MspA1I CmG'CkG
Cuts at: 1606 4383 5295 5312 5393 6125 6432 7109 8257
Size: 2777 912 17 81 732 307 677 1148
Cuts at: 8257 8502 1606
Size: 245 2120
Fragments arranged by size:
2777 2120 1148 912 732 677 307 245
81 17
NaeI GCC'GGC
Cuts at: 5641 5641
Size: 9016
NcoI C'CATG_G
Cuts at: 513 1065 2004 4059 6234 513
Size: 552 939 2055 2175 3295
Fragments arranged by size:
3295 2175 2055 939 552
NdeI CA'TA_TG
Cuts at: 387 2149 4657 6073 387
Size: 1762 2508 1416 3330
Fragments arranged by size:
3330 2508 1762 1416
NgoMIV G'CCGG_C
Cuts at: 5639 5639
Size: 9016
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9016
NotI GC'GGCC_GC
Cuts at: 6463 6463
Size: 9016
NsiI A_TGCA'T
Cuts at: 5964 5964
Size: 9016
NspI r_CATG'y
Cuts at: 1132 1229 3496 4519 5880 6374 7919 1132
Size: 97 2267 1023 1361 494 1545 2229
Fragments arranged by size:
2267 2229 1545 1361 1023 494 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 9016
PciI A'CATG_T
Cuts at: 3492 7915 3492
Size: 4423 4593
PflMI CCAn_nnn'nTGG
Cuts at: 1108 6074 1108
Size: 4966 4050
PfoI T'CCnGG_A
Cuts at: 5148 5148
Size: 9016
PmeI GTTT'AAAC
Cuts at: 6320 6320
Size: 9016
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7085 7117 8598 8630 7085
Size: 32 1481 32 7471
Fragments arranged by size:
7471 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9016
PsiI TTA'TAA
Cuts at: 3635 5787 6586 3635
Size: 2152 799 6065
Fragments arranged by size:
6065 2152 799
PspOMI G'GGCC_C
Cuts at: 6180 6180
Size: 9016
PstI C_TGCA'G
Cuts at: 830 3990 4033 5764 5846 6368 830
Size: 3160 43 1731 82 522 3478
Fragments arranged by size:
3478 3160 1731 522 82 43
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9016
PvuII CAG'CTG
Cuts at: 5312 5393 6125 5312
Size: 81 732 8203
Fragments arranged by size:
8203 732 81
SalI G'TCGA_C
Cuts at: 1936 6358 1936
Size: 4422 4594
SapI GCTCTTCn'nnn_
Cuts at: 2625 3381 3534 2625
Size: 756 153 8107
Fragments arranged by size:
8107 756 153
SbfI CC_TGCA'GG
Cuts at: 3990 5764 6368 3990
Size: 1774 604 6638
Fragments arranged by size:
6638 1774 604
ScaI AGT'ACT
Cuts at: 2733 3337 7264 8976 2733
Size: 604 3927 1712 2773
Fragments arranged by size:
3927 2773 1712 604
SfcI C'TryA_G
Cuts at: 826 1045 3540 3708 3834 3986 4029 4543 5760
Size: 219 2495 168 126 152 43 514 1217
Cuts at: 5760 5842 6364 7499 8180 8371 826
Size: 82 522 1135 681 191 1471
Fragments arranged by size:
2495 1471 1217 1135 681 522 514 219
191 168 152 126 82 43
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9016
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9016
SmaI CCC'GGG
Cuts at: 1872 6343 1872
Size: 4471 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2885 3150 3331 5203 7120 8021
Size: 197 926 942 265 181 1872 1917 901
Cuts at: 8021 8283 820
Size: 262 1553
Fragments arranged by size:
1917 1872 1553 942 926 901 265 262
197 181
SnaBI TAC'GTA
Cuts at: 493 6807 493
Size: 6314 2702
SpeI A'CTAG_T
Cuts at: 152 3932 5096 152
Size: 3780 1164 4072
Fragments arranged by size:
4072 3780 1164
SphI G_CATG'C
Cuts at: 1132 6374 1132
Size: 5242 3774
SspI AAT'ATT
Cuts at: 5 52 6940 5
Size: 47 6888 2081
Fragments arranged by size:
6888 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 9016
StyI C'CwwG_G
Cuts at: 513 1065 2004 2717 2832 2849 4059 5619 6234
Size: 552 939 713 115 17 1210 1560 615
Cuts at: 6234 6455 513
Size: 221 3074
Fragments arranged by size:
3074 1560 1210 939 713 615 552 221
115 17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4269 7043 7060 7213 7398 7843 325
Size: 1255 2689 2774 17 153 185 445 1498
Fragments arranged by size:
2774 2689 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2542 2731 3335
Size: 274 80 33 51 1443 565 189 604
Cuts at: 3335 7262 8974 96
Size: 3927 1712 138
Fragments arranged by size:
3927 1712 1443 604 565 274 189 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2634 2666 4950 4982 5327 5359 6077 6109 8976
Size: 32 2284 32 345 32 718 32 2867
Cuts at: 8976 9008 2634
Size: 32 2642
Fragments arranged by size:
2867 2642 2284 718 345 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9016
XbaI T'CTAG_A
Cuts at: 3968 6352 3968
Size: 2384 6632
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2961 4839 6078 2961
Size: 1878 1239 5899
Fragments arranged by size:
5899 1878 1239
XhoI C'TCGA_G
Cuts at: 1943 3150 1943
Size: 1207 7809
XmaI C'CCGG_G
Cuts at: 1870 6341 1870
Size: 4471 4545
XmnI GAAnn'nnTTC
Cuts at: 2199 3754 3822 5656 7145 2199
Size: 1555 68 1834 1489 4070
Fragments arranged by size:
4070 1834 1555 1489 68
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6824 276
Size: 53 83 186 936 5290 2468
Fragments arranged by size:
5290 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbeI BsiWI DraIII EcoICRI
EcoNI FseI FspAI KasI NarI NruI PacI PmlI
PpuMI PspXI PsrI RsrII SacI SacII SanDI SexAI
SfiI SfoI SrfI SwaI