Restriction map for
pFN21AB3492
(Circular) (Six-base) MAPSORT of: pFN21AB3492.seq Check: 1828 from: 1 to: 5896
pFN21AB3492 5896 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3706 278
Size: 53 83 186 936 2170 2468
Fragments arranged by size:
2468 2170 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2440 3239 1937
Size: 503 799 4594
Fragments arranged by size:
4594 799 503
Acc65I G'GTAC_C
Cuts at: 3217 3217
Size: 5896
AclI AA'CG_TT
Cuts at: 1563 4023 4396 1563
Size: 2460 373 3063
Fragments arranged by size:
3063 2460 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2742 3959 5343 5611 1632
Size: 1110 1217 1384 268 1917
Fragments arranged by size:
1917 1384 1217 1110 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5699
AflIII A'CryG_T
Cuts at: 1203 2179 4795 5772 1203
Size: 976 2616 977 1327
Fragments arranged by size:
2616 1327 977 976
AgeI A'CCGG_T
Cuts at: 4727 4727
Size: 5896
AhdI GACnn_n'nnGTC
Cuts at: 4625 4625
Size: 5896
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5896
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2281 3166 1184
Size: 1097 885 3914
Fragments arranged by size:
3914 1097 885
AlwNI CAG_nnn'CTG
Cuts at: 2024 2101 2810 3639 5211 2024
Size: 77 709 829 1572 2709
Fragments arranged by size:
2709 1572 829 709 77
ApaLI G'TGCA_C
Cuts at: 3953 5109 3953
Size: 1156 4740
ApoI r'AATT_y
Cuts at: 1493 2238 3087 3205 3431 3583 5692 1493
Size: 745 849 118 226 152 2109 1697
Fragments arranged by size:
2109 1697 849 745 226 152 118
AseI AT'TA_AT
Cuts at: 160 1032 4450 160
Size: 872 3418 1606
Fragments arranged by size:
3418 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5896
AvaI C'yCGr_G
Cuts at: 1870 1943 3221 1870
Size: 73 1278 4545
Fragments arranged by size:
4545 1278 73
BamHI G'GATC_C
Cuts at: 3226 3593 3226
Size: 367 5529
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2072 3217 4672 618
Size: 325 217 627 285 1145 1455 1842
Fragments arranged by size:
1842 1455 1145 627 325 285 217
BanII G_rGCy'C
Cuts at: 1391 2108 3215 1391
Size: 717 1107 4072
Fragments arranged by size:
4072 1107 717
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4925
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4087 4121 1903
Size: 34 2150 34 3678
Fragments arranged by size:
3678 2150 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3788 4998 3788
Size: 1210 4686
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5896
BfrBI ATG'CAT
Cuts at: 2465 2465
Size: 5896
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2144 4507 136
Size: 107 122 71 631 605 472 2363 1525
Fragments arranged by size:
2363 1525 631 605 472 122 107 71
BglII A'GATC_T
Cuts at: 5891 5891
Size: 5896
BlpI GC'TnA_GC
Cuts at: 3313 3313
Size: 5896
Bme1580I G_kGCm'C
Cuts at: 1790 3957 5113 1790
Size: 2167 1156 2573
Fragments arranged by size:
2573 2167 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5896
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3627 4585 442
Size: 416 53 83 399 24 2210 958 1753
Fragments arranged by size:
2210 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5896
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2394 2394
Size: 5896
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2320 3134 4556 1239
Size: 1081 814 1422 2579
Fragments arranged by size:
2579 1422 1081 814
Bpu10I CC'TnA_GC
Cuts at: 2077 2077
Size: 5896
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2416 4021 4886 5184 2416
Size: 1605 865 298 3128
Fragments arranged by size:
3128 1605 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4559 882
Size: 617 3060 2219
Fragments arranged by size:
3060 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3687 493
Size: 711 2483 2702
Fragments arranged by size:
2702 2483 711
BsaBI GATnn'nnATC
Cuts at: 5890 5890
Size: 5896
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3703 4085 4720 275
Size: 53 83 186 936 2170 382 635 1451
Fragments arranged by size:
2170 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2149 4329 4727 5001 5148 5559 2149
Size: 2180 398 274 147 411 2486
Fragments arranged by size:
2486 2180 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2090 2120 2131 2161 1178
Size: 30 165 30 687 30 11 30 4913
Fragments arranged by size:
4913 687 165 30 30 30 30 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2370 1186
Size: 1184 4712
BseYI C'CCAG_C
Cuts at: 2746 5099 5595 2746
Size: 2353 496 3047
Fragments arranged by size:
3047 2353 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2253 1163
Size: 573 517 4806
Fragments arranged by size:
4806 573 517
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3346 4107 5135 1540
Size: 275 186 1345 761 1028 2301
Fragments arranged by size:
2301 1345 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3957 4042 5113 3957
Size: 85 1071 4740
Fragments arranged by size:
4740 1071 85
BsmI GAATG_Cn'
Cuts at: 2014 2581 3407 3500 2014
Size: 567 826 93 4410
Fragments arranged by size:
4410 826 567 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2041 2041
Size: 5896
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2108 3215 3957 4042 5113 1391
Size: 399 318 1107 742 85 1071 2174
Fragments arranged by size:
2174 1107 1071 742 399 318 85
BspEI T'CCGG_A
Cuts at: 2149 5559 2149
Size: 3410 2486
BspHI T'CATG_A
Cuts at: 3785 5515 3785
Size: 1730 4166
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2918 3251 844
Size: 2074 333 3489
Fragments arranged by size:
3489 2074 333
BsrBI CCG'CTC
Cuts at: 1394 1823 3783 1394
Size: 429 1960 3507
Fragments arranged by size:
3507 1960 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3183 4391 4565 65
Size: 1183 1935 1208 174 1396
Fragments arranged by size:
1935 1396 1208 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4540 4727 1700
Size: 110 2730 187 2869
Fragments arranged by size:
2869 2730 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5896
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5896
BssSI C'ACGA_G
Cuts at: 3956 4968 3956
Size: 1012 4884
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5896
BstBI TT'CG_AA
Cuts at: 4700 4700
Size: 5896
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5896
BstXI CCAn_nnnn'nTGG
Cuts at: 2344 3068 2344
Size: 724 5172
BstYI r'GATC_y
Cuts at: 1972 2020 3226 3593 3978 3995 5447 5533 5545
Size: 48 1206 367 385 17 1452 86 12
Cuts at: 5545 5891 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1206 385 367 346 86 48
17 12
BstZ17I GTA'TAC
Cuts at: 2441 2441
Size: 5896
BtgI C'CryG_G
Cuts at: 513 1065 2674 513
Size: 552 1609 3735
Fragments arranged by size:
3735 1609 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5157
Fragments arranged by size:
5157 683 56
BtsI GCAGTG_nn'
Cuts at: 3412 4206 4226 3412
Size: 794 20 5082
Fragments arranged by size:
5082 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5896
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5861
DraI TTT'AAA
Cuts at: 3200 3200
Size: 5896
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4903 809
Size: 507 3587 1802
Fragments arranged by size:
3587 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2590 3343 4232
Size: 54 1425 50 275 18 760 753 889
Cuts at: 4232 5861 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 760 753 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3343 1537
Size: 275 1531 4090
Fragments arranged by size:
4090 1531 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2126 2629 3826 1353
Size: 773 503 1197 3423
Fragments arranged by size:
3423 1197 773 503
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4483 4857 5003 387
Size: 1064 138 2894 374 146 1280
Fragments arranged by size:
2894 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2320 2742 3134 3959 4556 5343 5611
Size: 393 688 422 392 825 597 787 268
Cuts at: 5611 1239
Size: 1524
Fragments arranged by size:
1524 825 787 688 597 422 393 392
268
EcoO109I rG'GnC_Cy
Cuts at: 2098 2098
Size: 5896
EcoRI G'AATT_C
Cuts at: 2238 3087 3205 3583 2238
Size: 849 118 378 4551
Fragments arranged by size:
4551 849 378 118
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2731 2731
Size: 5896
FspI TGC'GCA
Cuts at: 4402 4402
Size: 5896
HaeII r_GCGC'y
Cuts at: 1678 5043 1678
Size: 3365 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2090 2122 4537 4569 4611
Size: 32 135 32 518 32 2415 32 42
Cuts at: 4611 4643 1373
Size: 32 2626
Fragments arranged by size:
2626 2415 518 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2628 2950 3240 3486 669
Size: 988 281 690 322 290 246 3079
Fragments arranged by size:
3079 988 690 322 290 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5896
HpaI GTT'AAC
Cuts at: 3486 3486
Size: 5896
KpnI G_GTAC'C
Cuts at: 3221 3221
Size: 5896
MfeI C'AATT_G
Cuts at: 3495 3495
Size: 5896
MluI A'CGCG_T
Cuts at: 5772 5772
Size: 5896
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5010 5194 1305
Size: 3705 184 2007
Fragments arranged by size:
3705 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2592 10
Size: 54 1425 343 760 3314
Fragments arranged by size:
3314 1425 760 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3854 4213 4372 518
Size: 648 2688 359 159 2042
Fragments arranged by size:
2688 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2495 2783 2981 3312 3989 5137 5382 1606
Size: 889 288 198 331 677 1148 245 2120
Fragments arranged by size:
2120 1148 889 677 331 288 245 198
NcoI C'CATG_G
Cuts at: 513 1065 2674 513
Size: 552 1609 3735
Fragments arranged by size:
3735 1609 552
NdeI CA'TA_TG
Cuts at: 387 2481 387
Size: 2094 3802
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5896
NotI GC'GGCC_GC
Cuts at: 3343 3343
Size: 5896
NsiI A_TGCA'T
Cuts at: 2467 2467
Size: 5896
NspI r_CATG'y
Cuts at: 1132 1229 2183 3254 4799 1132
Size: 97 954 1071 1545 2229
Fragments arranged by size:
2229 1545 1071 954 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5896
PciI A'CATG_T
Cuts at: 2179 4795 2179
Size: 2616 3280
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2101 2514 1108
Size: 993 413 4490
Fragments arranged by size:
4490 993 413
PfoI T'CCnGG_A
Cuts at: 2094 2094
Size: 5896
PmeI GTTT'AAAC
Cuts at: 3200 3200
Size: 5896
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3965 3997 5478 5510 3965
Size: 32 1481 32 4351
Fragments arranged by size:
4351 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2098 2098
Size: 5896
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5896
PsiI TTA'TAA
Cuts at: 3466 3466
Size: 5896
PstI C_TGCA'G
Cuts at: 830 2782 3248 830
Size: 1952 466 3478
Fragments arranged by size:
3478 1952 466
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5896
PvuII CAG'CTG
Cuts at: 2495 2783 2981 2495
Size: 288 198 5410
Fragments arranged by size:
5410 288 198
SalI G'TCGA_C
Cuts at: 1936 3238 1936
Size: 1302 4594
SbfI CC_TGCA'GG
Cuts at: 3248 3248
Size: 5896
ScaI AGT'ACT
Cuts at: 4144 5856 4144
Size: 1712 4184
SfcI C'TryA_G
Cuts at: 826 1045 2778 3048 3244 4379 5060 5251 826
Size: 219 1733 270 196 1135 681 191 1471
Fragments arranged by size:
1733 1471 1135 681 270 219 196 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5896
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5896
SmaI CCC'GGG
Cuts at: 1872 3223 1872
Size: 1351 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2395 4000 4901 5163 820
Size: 197 926 452 1605 901 262 1553
Fragments arranged by size:
1605 1553 926 901 452 262 197
SnaBI TAC'GTA
Cuts at: 493 3687 493
Size: 3194 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5896
SphI G_CATG'C
Cuts at: 1132 3254 1132
Size: 2122 3774
SspI AAT'ATT
Cuts at: 5 52 3034 3820 5
Size: 47 2982 786 2081
Fragments arranged by size:
2982 2081 786 47
StuI AGG'CCT
Cuts at: 1517 2289 1517
Size: 772 5124
StyI C'CwwG_G
Cuts at: 513 1065 2674 3054 3335 513
Size: 552 1609 380 281 3074
Fragments arranged by size:
3074 1609 552 380 281
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2219 2852 3923 3940 4093 4278 4723
Size: 1255 639 633 1071 17 153 185 445
Cuts at: 4723 325
Size: 1498
Fragments arranged by size:
1498 1255 1071 639 633 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4142 5854 96
Size: 274 80 33 51 1443 2165 1712 138
Fragments arranged by size:
2165 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2131 2163 5856 5888 2131
Size: 32 3693 32 2139
Fragments arranged by size:
3693 2139 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5896
XbaI T'CTAG_A
Cuts at: 3232 3232
Size: 5896
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2276 2276
Size: 5896
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5896
XmaI C'CCGG_G
Cuts at: 1870 3221 1870
Size: 1351 4545
XmnI GAAnn'nnTTC
Cuts at: 4025 4025
Size: 5896
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3704 276
Size: 53 83 186 936 2170 2468
Fragments arranged by size:
2468 2170 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BsiWI Bsu36I DraIII EcoICRI EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI