Restriction map for
pFN21AB3489
(Circular) (Six-base) MAPSORT of: pFN21AB3489.seq Check: 6018 from: 1 to: 5701
pFN21AB3489 5701 bp
With 182 enzymes: SgfI *
October 20, 2009 10:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2872 2872
Size: 5701
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3511 278
Size: 53 83 186 936 1975 2468
Fragments arranged by size:
2468 1975 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2631 3044 1937
Size: 694 413 4594
Fragments arranged by size:
4594 694 413
Acc65I G'GTAC_C
Cuts at: 3022 3022
Size: 5701
AclI AA'CG_TT
Cuts at: 1563 3828 4201 1563
Size: 2265 373 3063
Fragments arranged by size:
3063 2265 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3764 5148 5416 1632
Size: 2132 1384 268 1917
Fragments arranged by size:
2132 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5504
AflIII A'CryG_T
Cuts at: 1203 4600 5577 1203
Size: 3397 977 1327
Fragments arranged by size:
3397 1327 977
AgeI A'CCGG_T
Cuts at: 4532 4532
Size: 5701
AhdI GACnn_n'nnGTC
Cuts at: 4430 4430
Size: 5701
AleI CACnn'nnGTG
Cuts at: 1166 2732 1166
Size: 1566 4135
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5701
AlwNI CAG_nnn'CTG
Cuts at: 3444 5016 3444
Size: 1572 4129
ApaLI G'TGCA_C
Cuts at: 3758 4914 3758
Size: 1156 4545
ApoI r'AATT_y
Cuts at: 1493 2390 2788 3010 3236 3388 5497 1493
Size: 897 398 222 226 152 2109 1697
Fragments arranged by size:
2109 1697 897 398 226 222 152
AseI AT'TA_AT
Cuts at: 160 1032 2247 4255 160
Size: 872 1215 2008 1606
Fragments arranged by size:
2008 1606 1215 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5701
AvaI C'yCGr_G
Cuts at: 1870 1943 2096 3026 1870
Size: 73 153 930 4545
Fragments arranged by size:
4545 930 153 73
BamHI G'GATC_C
Cuts at: 3031 3398 3031
Size: 367 5334
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3022 4477 618
Size: 325 217 627 1235 1455 1842
Fragments arranged by size:
1842 1455 1235 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2877 3020 1391
Size: 1486 143 4072
Fragments arranged by size:
4072 1486 143
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4730
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2134 2168 3892 3926 1903
Size: 34 50 34 113 34 1724 34 3678
Fragments arranged by size:
3678 1724 113 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3593 4803 3593
Size: 1210 4491
BclI T'GATC_A
Cuts at: 1548 2472 1548
Size: 924 4777
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2499 4312 136
Size: 107 122 71 631 605 827 1813 1525
Fragments arranged by size:
1813 1525 827 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2398 5696 2398
Size: 3298 2403
BlpI GC'TnA_GC
Cuts at: 3118 3118
Size: 5701
Bme1580I G_kGCm'C
Cuts at: 1790 3762 4918 1790
Size: 1972 1156 2573
Fragments arranged by size:
2573 1972 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5701
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2348 3432 4390
Size: 416 53 83 399 24 931 1084 958
Cuts at: 4390 442
Size: 1753
Fragments arranged by size:
1753 1084 958 931 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5701
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2835 2861 4361 1239
Size: 1596 26 1500 2579
Fragments arranged by size:
2579 1596 1500 26
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2274 3826 4691 4989 2274
Size: 1552 865 298 2986
Fragments arranged by size:
2986 1552 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2717 4364 882
Size: 617 1218 1647 2219
Fragments arranged by size:
2219 1647 1218 617
BsaAI yAC'GTr
Cuts at: 493 1204 3492 493
Size: 711 2288 2702
Fragments arranged by size:
2702 2288 711
BsaBI GATnn'nnATC
Cuts at: 5695 5695
Size: 5701
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3508 3890 4525 275
Size: 53 83 186 936 1975 382 635 1451
Fragments arranged by size:
1975 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4134 4532 4806 4953 5364 4134
Size: 398 274 147 411 4471
Fragments arranged by size:
4471 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5476
Fragments arranged by size:
5476 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5701
BseYI C'CCAG_C
Cuts at: 2459 4904 5400 2459
Size: 2445 496 2760
Fragments arranged by size:
2760 2445 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2388 1163
Size: 573 652 4476
Fragments arranged by size:
4476 652 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3151 3912 4940 1540
Size: 275 186 1150 761 1028 2301
Fragments arranged by size:
2301 1150 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3762 3847 4918 3762
Size: 85 1071 4545
Fragments arranged by size:
4545 1071 85
BsmI GAATG_Cn'
Cuts at: 3212 3305 3212
Size: 93 5608
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2877 3020 3762 3847 4918 1391
Size: 399 1087 143 742 85 1071 2174
Fragments arranged by size:
2174 1087 1071 742 399 143 85
BspEI T'CCGG_A
Cuts at: 5364 5364
Size: 5701
BspHI T'CATG_A
Cuts at: 3590 5320 3590
Size: 1730 3971
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2872 3056 844
Size: 2028 184 3489
Fragments arranged by size:
3489 2028 184
BsrBI CCG'CTC
Cuts at: 1394 1823 2068 3588 1394
Size: 429 245 1520 3507
Fragments arranged by size:
3507 1520 429 245
BsrDI GCAATG_nn'
Cuts at: 65 1248 2145 4196 4370 65
Size: 1183 897 2051 174 1396
Fragments arranged by size:
2051 1396 1183 897 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4345 4532 1700
Size: 110 2535 187 2869
Fragments arranged by size:
2869 2535 187 110
BsrGI T'GTAC_A
Cuts at: 96 2052 96
Size: 1956 3745
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5701
BssSI C'ACGA_G
Cuts at: 2030 3761 4773 2030
Size: 1731 1012 2958
Fragments arranged by size:
2958 1731 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5701
BstBI TT'CG_AA
Cuts at: 4505 4505
Size: 5701
BstEII G'GTnAC_C
Cuts at: 1184 2887 1184
Size: 1703 3998
BstYI r'GATC_y
Cuts at: 1972 2398 3031 3398 3783 3800 5252 5338 5350
Size: 426 633 367 385 17 1452 86 12
Cuts at: 5350 5696 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 633 426 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2892 513
Size: 552 1827 3322
Fragments arranged by size:
3322 1827 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4962
Fragments arranged by size:
4962 683 56
BtsI GCAGTG_nn'
Cuts at: 2385 3217 4011 4031 2385
Size: 832 794 20 4055
Fragments arranged by size:
4055 832 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5701
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5666
DraI TTT'AAA
Cuts at: 3005 3005
Size: 5701
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4708 809
Size: 507 3392 1802
Fragments arranged by size:
3392 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2754 3148 4037
Size: 54 1425 50 275 18 924 394 889
Cuts at: 4037 5666 8
Size: 1629 43
Fragments arranged by size:
1629 1425 924 889 394 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3148 1537
Size: 275 1336 4090
Fragments arranged by size:
4090 1336 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3631 1353
Size: 2278 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4288 4662 4808 387
Size: 1064 138 2699 374 146 1280
Fragments arranged by size:
2699 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2835 2861 3764 4361 5148 5416 1239
Size: 393 1203 26 903 597 787 268 1524
Fragments arranged by size:
1524 1203 903 787 597 393 268 26
EcoNI CCTnn'n_nnAGG
Cuts at: 2419 2419
Size: 5701
EcoRI G'AATT_C
Cuts at: 3010 3388 3010
Size: 378 5323
FspI TGC'GCA
Cuts at: 4207 4207
Size: 5701
HaeII r_GCGC'y
Cuts at: 1678 2494 4848 1678
Size: 816 2354 2531
Fragments arranged by size:
2531 2354 816
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2714 2746 4342 4374 4416
Size: 32 135 32 1142 32 1596 32 42
Cuts at: 4416 4448 1373
Size: 32 2626
Fragments arranged by size:
2626 1596 1142 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3045 3291 669
Size: 988 281 1107 246 3079
Fragments arranged by size:
3079 1107 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2598 748
Size: 1850 3851
HpaI GTT'AAC
Cuts at: 3291 3291
Size: 5701
KpnI G_GTAC'C
Cuts at: 3026 3026
Size: 5701
MfeI C'AATT_G
Cuts at: 3300 3300
Size: 5701
MluI A'CGCG_T
Cuts at: 5577 5577
Size: 5701
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4815 4999 1305
Size: 3510 184 2007
Fragments arranged by size:
3510 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2756 10
Size: 54 1425 343 924 2955
Fragments arranged by size:
2955 1425 924 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2480 2732 2734 3659 4018 4177 518
Size: 648 1314 252 2 925 359 159 2042
Fragments arranged by size:
2042 1314 925 648 359 252 159 2
MspA1I CmG'CkG
Cuts at: 1606 3117 3794 4942 5187 1606
Size: 1511 677 1148 245 2120
Fragments arranged by size:
2120 1511 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2892 513
Size: 552 1827 3322
Fragments arranged by size:
3322 1827 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5701
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5701
NotI GC'GGCC_GC
Cuts at: 3148 3148
Size: 5701
NspI r_CATG'y
Cuts at: 1132 1229 2108 2356 2824 3059 4604 1132
Size: 97 879 248 468 235 1545 2229
Fragments arranged by size:
2229 1545 879 468 248 235 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5701
PciI A'CATG_T
Cuts at: 4600 4600
Size: 5701
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2884 1108
Size: 1776 3925
PmeI GTTT'AAAC
Cuts at: 3005 3005
Size: 5701
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3770 3802 5283 5315 3770
Size: 32 1481 32 4156
Fragments arranged by size:
4156 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5701
PsiI TTA'TAA
Cuts at: 2180 3271 2180
Size: 1091 4610
PstI C_TGCA'G
Cuts at: 830 2217 3053 830
Size: 1387 836 3478
Fragments arranged by size:
3478 1387 836
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5701
SalI G'TCGA_C
Cuts at: 1936 3043 1936
Size: 1107 4594
SbfI CC_TGCA'GG
Cuts at: 3053 3053
Size: 5701
ScaI AGT'ACT
Cuts at: 3949 5661 3949
Size: 1712 3989
SfcI C'TryA_G
Cuts at: 826 1045 2213 3049 4184 4865 5056 826
Size: 219 1168 836 1135 681 191 1471
Fragments arranged by size:
1471 1168 1135 836 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5701
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5701
SmaI CCC'GGG
Cuts at: 1872 2098 3028 1872
Size: 226 930 4545
Fragments arranged by size:
4545 930 226
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2289 3805 4706 4968 820
Size: 197 926 346 1516 901 262 1553
Fragments arranged by size:
1553 1516 926 901 346 262 197
SnaBI TAC'GTA
Cuts at: 493 3492 493
Size: 2999 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5701
SphI G_CATG'C
Cuts at: 1132 3059 1132
Size: 1927 3774
SspI AAT'ATT
Cuts at: 5 52 3625 5
Size: 47 3573 2081
Fragments arranged by size:
3573 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5701
StyI C'CwwG_G
Cuts at: 513 1065 2803 2892 2926 3140 513
Size: 552 1738 89 34 214 3074
Fragments arranged by size:
3074 1738 552 214 89 34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3728 3745 3898 4083 4528 325
Size: 1255 2148 17 153 185 445 1498
Fragments arranged by size:
2148 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2052 3947 5659
Size: 274 80 33 51 1443 75 1895 1712
Cuts at: 5659 96
Size: 138
Fragments arranged by size:
1895 1712 1443 274 138 80 75 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2364 2396 5661 5693 2364
Size: 32 3265 32 2372
Fragments arranged by size:
3265 2372 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5701
XbaI T'CTAG_A
Cuts at: 3037 3037
Size: 5701
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2587 2011
Size: 576 5125
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5701
XmaI C'CCGG_G
Cuts at: 1870 2096 3026 1870
Size: 226 930 4545
Fragments arranged by size:
4545 930 226
XmnI GAAnn'nnTTC
Cuts at: 3830 3830
Size: 5701
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3509 276
Size: 53 83 186 936 1975 2468
Fragments arranged by size:
2468 1975 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI