Restriction map for
pFN21AB3486
(Circular) (Six-base) MAPSORT of: pFN21AB3486.seq Check: 6517 from: 1 to: 5809
pFN21AB3486 5809 bp
With 182 enzymes: SgfI *
October 20, 2009 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2626 2626
Size: 5809
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3000 3619 278
Size: 53 83 186 936 1464 619 2468
Fragments arranged by size:
2468 1464 936 619 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3152 1937
Size: 1215 4594
Acc65I G'GTAC_C
Cuts at: 3130 3130
Size: 5809
AclI AA'CG_TT
Cuts at: 1563 3936 4309 1563
Size: 2373 373 3063
Fragments arranged by size:
3063 2373 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2042 2477 2498 3872 5256 5524 1632
Size: 410 435 21 1374 1384 268 1917
Fragments arranged by size:
1917 1384 1374 435 410 268 21
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5612
AflIII A'CryG_T
Cuts at: 1203 4708 5685 1203
Size: 3505 977 1327
Fragments arranged by size:
3505 1327 977
AgeI A'CCGG_T
Cuts at: 4640 4640
Size: 5809
AhdI GACnn_n'nnGTC
Cuts at: 3055 4538 3055
Size: 1483 4326
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5809
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5809
AlwNI CAG_nnn'CTG
Cuts at: 2440 2563 2701 3552 5124 2440
Size: 123 138 851 1572 3125
Fragments arranged by size:
3125 1572 851 138 123
ApaLI G'TGCA_C
Cuts at: 3866 5022 3866
Size: 1156 4653
ApoI r'AATT_y
Cuts at: 1493 3118 3344 3496 5605 1493
Size: 1625 226 152 2109 1697
Fragments arranged by size:
2109 1697 1625 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4363 160
Size: 872 3331 1606
Fragments arranged by size:
3331 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5809
AvaI C'yCGr_G
Cuts at: 1870 1943 2781 2960 3134 1870
Size: 73 838 179 174 4545
Fragments arranged by size:
4545 838 179 174 73
BamHI G'GATC_C
Cuts at: 3139 3506 3139
Size: 367 5442
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3130 4585 618
Size: 325 217 627 1343 1455 1842
Fragments arranged by size:
1842 1455 1343 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2790 2961 3128 1391
Size: 1399 171 167 4072
Fragments arranged by size:
4072 1399 171 167
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4838
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2844 2878 4000 4034 1903
Size: 34 907 34 1122 34 3678
Fragments arranged by size:
3678 1122 907 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3701 4911 3701
Size: 1210 4599
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5809
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2198 3060 4420
Size: 107 122 71 631 605 526 862 1360
Cuts at: 4420 136
Size: 1525
Fragments arranged by size:
1525 1360 862 631 605 526 122 107
71
BglII A'GATC_T
Cuts at: 5804 5804
Size: 5809
BlpI GC'TnA_GC
Cuts at: 2202 3074 3226 2202
Size: 872 152 4785
Fragments arranged by size:
4785 872 152
Bme1580I G_kGCm'C
Cuts at: 1790 3099 3870 5026 1790
Size: 1309 771 1156 2573
Fragments arranged by size:
2573 1309 1156 771
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5809
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3540 4498 442
Size: 416 53 83 399 24 2123 958 1753
Fragments arranged by size:
2123 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5809
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2417 2507 2585 2639 4469 1239
Size: 1178 90 78 54 1830 2579
Fragments arranged by size:
2579 1830 1178 90 78 54
Bpu10I CC'TnA_GC
Cuts at: 2757 2808 2757
Size: 51 5758
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2708 3934 4799 5097 2708
Size: 1226 865 298 3420
Fragments arranged by size:
3420 1226 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4472 882
Size: 617 2973 2219
Fragments arranged by size:
2973 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3082 3600 493
Size: 711 1878 518 2702
Fragments arranged by size:
2702 1878 711 518
BsaBI GATnn'nnATC
Cuts at: 5803 5803
Size: 5809
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2997 3616 3998 4633
Size: 53 83 186 936 1464 619 382 635
Cuts at: 4633 275
Size: 1451
Fragments arranged by size:
1464 1451 936 635 619 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2892 4242 4640 4914 5061 5472 2892
Size: 1350 398 274 147 411 3229
Fragments arranged by size:
3229 1350 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5584
Fragments arranged by size:
5584 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2561 2591 1186
Size: 1375 30 4404
Fragments arranged by size:
4404 1375 30
BseYI C'CCAG_C
Cuts at: 2188 2314 2977 5012 5508 2188
Size: 126 663 2035 496 2489
Fragments arranged by size:
2489 2035 663 496 126
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2714 2818 1163
Size: 573 978 104 4154
Fragments arranged by size:
4154 978 573 104
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3259 4020 5048 1540
Size: 275 186 1258 761 1028 2301
Fragments arranged by size:
2301 1258 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3870 3955 5026 3870
Size: 85 1071 4653
Fragments arranged by size:
4653 1071 85
BsmI GAATG_Cn'
Cuts at: 3320 3413 3320
Size: 93 5716
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2790 2961 3099 3128 3870 3955 5026
Size: 399 1000 171 138 29 742 85 1071
Cuts at: 5026 1391
Size: 2174
Fragments arranged by size:
2174 1071 1000 742 399 171 138 85
29
BspEI T'CCGG_A
Cuts at: 2892 5472 2892
Size: 2580 3229
BspHI T'CATG_A
Cuts at: 3698 5428 3698
Size: 1730 4079
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2184 2626 3097 3164 844
Size: 1340 442 471 67 3489
Fragments arranged by size:
3489 1340 471 442 67
BsrBI CCG'CTC
Cuts at: 1394 1823 3073 3696 1394
Size: 429 1250 623 3507
Fragments arranged by size:
3507 1250 623 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2342 4304 4478 65
Size: 1183 1094 1962 174 1396
Fragments arranged by size:
1962 1396 1183 1094 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2354 2682 4453 4640 1700
Size: 110 544 328 1771 187 2869
Fragments arranged by size:
2869 1771 544 328 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5809
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5809
BssSI C'ACGA_G
Cuts at: 3869 4881 3869
Size: 1012 4797
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2692 794
Size: 1898 3911
BstBI TT'CG_AA
Cuts at: 4613 4613
Size: 5809
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5809
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2590 2011
Size: 579 5230
BstYI r'GATC_y
Cuts at: 1972 2232 2463 2535 2697 3139 3506 3891 3908
Size: 260 231 72 162 442 367 385 17
Cuts at: 3908 5360 5446 5458 5804 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 442 385 367 346 260 231
162 86 72 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2302 513
Size: 552 1237 4020
Fragments arranged by size:
4020 1237 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5070
Fragments arranged by size:
5070 683 56
BtsI GCAGTG_nn'
Cuts at: 3325 4119 4139 3325
Size: 794 20 4995
Fragments arranged by size:
4995 794 20
ClaI AT'CG_AT
Cuts at: 1554 3021 1554
Size: 1467 4342
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5774
DraI TTT'AAA
Cuts at: 3113 3113
Size: 5809
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2405 4816 809
Size: 507 1089 2411 1802
Fragments arranged by size:
2411 1802 1089 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2157 3052 3256
Size: 54 1425 50 275 18 327 895 204
Cuts at: 3256 4145 5774 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 895 889 327 275 204 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3256 1537
Size: 275 1444 4090
Fragments arranged by size:
4090 1444 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3739 1353
Size: 2386 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2288 2729 4396 4770 4916 387
Size: 1064 138 699 441 1667 374 146 1280
Fragments arranged by size:
1667 1280 1064 699 441 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2042 2417 2477 2498 2507 2585 2639
Size: 393 410 375 60 21 9 78 54
Cuts at: 2639 3872 4469 5256 5524 1239
Size: 1233 597 787 268 1524
Fragments arranged by size:
1524 1233 787 597 410 393 375 268
78 60 54 21 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2378 3104 2378
Size: 726 5083
EcoRI G'AATT_C
Cuts at: 3118 3496 3118
Size: 378 5431
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2434 2434
Size: 5809
FspI TGC'GCA
Cuts at: 4315 4315
Size: 5809
HaeII r_GCGC'y
Cuts at: 1678 2374 2564 2945 4956 1678
Size: 696 190 381 2011 2531
Fragments arranged by size:
2531 2011 696 381 190
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3041 3073 4450 4482 4524
Size: 32 135 32 1469 32 1377 32 42
Cuts at: 4524 4556 1373
Size: 32 2626
Fragments arranged by size:
2626 1469 1377 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3153 3399 669
Size: 988 281 1215 246 3079
Fragments arranged by size:
3079 1215 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2742 748
Size: 1994 3815
HpaI GTT'AAC
Cuts at: 3399 3399
Size: 5809
KpnI G_GTAC'C
Cuts at: 3134 3134
Size: 5809
MfeI C'AATT_G
Cuts at: 3408 3408
Size: 5809
MluI A'CGCG_T
Cuts at: 5685 5685
Size: 5809
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2047 2250 2352 2841 2904 4923 5107 1305
Size: 742 203 102 489 63 2019 184 2007
Fragments arranged by size:
2019 2007 742 489 203 184 102 63
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2159 10
Size: 54 1425 343 327 3660
Fragments arranged by size:
3660 1425 343 327 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3767 4126 4285 518
Size: 648 843 1758 359 159 2042
Fragments arranged by size:
2042 1758 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2050 2671 3225 3902 5050 5295 1606
Size: 444 621 554 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 621 554 444 245
NaeI GCC'GGC
Cuts at: 2356 2356
Size: 5809
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5257
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5809
NgoMIV G'CCGG_C
Cuts at: 2354 2354
Size: 5809
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5809
NotI GC'GGCC_GC
Cuts at: 3256 3256
Size: 5809
NspI r_CATG'y
Cuts at: 1132 1229 2608 2914 3046 3167 4712 1132
Size: 97 1379 306 132 121 1545 2229
Fragments arranged by size:
2229 1545 1379 306 132 121 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5809
PciI A'CATG_T
Cuts at: 4708 4708
Size: 5809
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2563 1108
Size: 1455 4354
PfoI T'CCnGG_A
Cuts at: 2616 2700 2616
Size: 84 5725
PmeI GTTT'AAAC
Cuts at: 3113 3113
Size: 5809
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3878 3910 5391 5423 3878
Size: 32 1481 32 4264
Fragments arranged by size:
4264 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5809
PsiI TTA'TAA
Cuts at: 3379 3379
Size: 5809
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2243 2275 2243
Size: 32 5777
PstI C_TGCA'G
Cuts at: 830 2181 3161 830
Size: 1351 980 3478
Fragments arranged by size:
3478 1351 980
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5809
PvuII CAG'CTG
Cuts at: 2050 2671 2050
Size: 621 5188
SalI G'TCGA_C
Cuts at: 1936 3151 1936
Size: 1215 4594
SbfI CC_TGCA'GG
Cuts at: 3161 3161
Size: 5809
ScaI AGT'ACT
Cuts at: 4057 5769 4057
Size: 1712 4097
SfcI C'TryA_G
Cuts at: 826 1045 2177 3157 4292 4973 5164 826
Size: 219 1132 980 1135 681 191 1471
Fragments arranged by size:
1471 1135 1132 980 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5809
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5809
SmaI CCC'GGG
Cuts at: 1872 2783 3136 1872
Size: 911 353 4545
Fragments arranged by size:
4545 911 353
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2723 3913 4814 5076 820
Size: 197 926 780 1190 901 262 1553
Fragments arranged by size:
1553 1190 926 901 780 262 197
SnaBI TAC'GTA
Cuts at: 493 3600 493
Size: 3107 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5809
SphI G_CATG'C
Cuts at: 1132 2608 2914 3046 3167 1132
Size: 1476 306 132 121 3774
Fragments arranged by size:
3774 1476 306 132 121
SspI AAT'ATT
Cuts at: 5 52 3733 5
Size: 47 3681 2081
Fragments arranged by size:
3681 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5809
StyI C'CwwG_G
Cuts at: 513 1065 2583 2797 3248 513
Size: 552 1518 214 451 3074
Fragments arranged by size:
3074 1518 552 451 214
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3836 3853 4006 4191 4636 325
Size: 1255 2256 17 153 185 445 1498
Fragments arranged by size:
2256 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4055 5767 96
Size: 274 80 33 51 1443 2078 1712 138
Fragments arranged by size:
2078 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5769 5801 5769
Size: 32 5777
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5809
XbaI T'CTAG_A
Cuts at: 3145 3145
Size: 5809
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2371 2371
Size: 5809
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5809
XmaI C'CCGG_G
Cuts at: 1870 2781 3134 1870
Size: 911 353 4545
Fragments arranged by size:
4545 911 353
XmnI GAAnn'nnTTC
Cuts at: 2030 3938 2030
Size: 1908 3901
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2998 3617 276
Size: 53 83 186 936 1464 619 2468
Fragments arranged by size:
2468 1464 936 619 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoO109I EcoRV FseI FspAI KasI NarI NruI NsiI
PacI PmlI PpuMI PspOMI PspXI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI