Restriction map for
pFN21AB3483
(Circular) (Six-base) MAPSORT of: pFN21AB3483.seq Check: 8375 from: 1 to: 5464
pFN21AB3483 5464 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3274 278
Size: 53 83 186 936 1738 2468
Fragments arranged by size:
2468 1738 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2807 1937
Size: 870 4594
Acc65I G'GTAC_C
Cuts at: 2785 2785
Size: 5464
AclI AA'CG_TT
Cuts at: 1563 3591 3964 1563
Size: 2028 373 3063
Fragments arranged by size:
3063 2028 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3527 4911 5179 1632
Size: 1895 1384 268 1917
Fragments arranged by size:
1917 1895 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5267
AflIII A'CryG_T
Cuts at: 1203 4363 5340 1203
Size: 3160 977 1327
Fragments arranged by size:
3160 1327 977
AgeI A'CCGG_T
Cuts at: 4295 4295
Size: 5464
AhdI GACnn_n'nnGTC
Cuts at: 4193 4193
Size: 5464
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5464
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5464
AlwNI CAG_nnn'CTG
Cuts at: 2357 3207 4779 2357
Size: 850 1572 3042
Fragments arranged by size:
3042 1572 850
ApaLI G'TGCA_C
Cuts at: 2531 3521 4677 2531
Size: 990 1156 3318
Fragments arranged by size:
3318 1156 990
ApoI r'AATT_y
Cuts at: 1493 2773 2999 3151 5260 1493
Size: 1280 226 152 2109 1697
Fragments arranged by size:
2109 1697 1280 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4018 160
Size: 872 2986 1606
Fragments arranged by size:
2986 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5464
AvaI C'yCGr_G
Cuts at: 1870 1943 2119 2789 1870
Size: 73 176 670 4545
Fragments arranged by size:
4545 670 176 73
BamHI G'GATC_C
Cuts at: 2794 3161 2794
Size: 367 5097
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2285 2785 4240 618
Size: 325 217 627 498 500 1455 1842
Fragments arranged by size:
1842 1455 627 500 498 325 217
BanII G_rGCy'C
Cuts at: 1391 2783 1391
Size: 1392 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4493
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3655 3689 1903
Size: 34 1718 34 3678
Fragments arranged by size:
3678 1718 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3356 4566 3356
Size: 1210 4254
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5464
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2139 2382 4075
Size: 107 122 71 631 605 467 243 1693
Cuts at: 4075 136
Size: 1525
Fragments arranged by size:
1693 1525 631 605 467 243 122 107
71
BglII A'GATC_T
Cuts at: 5459 5459
Size: 5464
BlpI GC'TnA_GC
Cuts at: 2881 2881
Size: 5464
Bme1580I G_kGCm'C
Cuts at: 1790 2535 3525 4681 1790
Size: 745 990 1156 2573
Fragments arranged by size:
2573 1156 990 745
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5464
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2112 2647 3195
Size: 416 53 83 399 24 695 535 548
Cuts at: 3195 4153 442
Size: 958 1753
Fragments arranged by size:
1753 958 695 548 535 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5464
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2685 2685
Size: 5464
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2109 2157 4124 1239
Size: 870 48 1967 2579
Fragments arranged by size:
2579 1967 870 48
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3589 4454 4752 3589
Size: 865 298 4301
Fragments arranged by size:
4301 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2597 4127 882
Size: 617 1098 1530 2219
Fragments arranged by size:
2219 1530 1098 617
BsaAI yAC'GTr
Cuts at: 493 1204 3255 493
Size: 711 2051 2702
Fragments arranged by size:
2702 2051 711
BsaBI GATnn'nnATC
Cuts at: 2645 5458 2645
Size: 2813 2651
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3271 3653 4288 275
Size: 53 83 186 936 1738 382 635 1451
Fragments arranged by size:
1738 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3897 4295 4569 4716 5127 3897
Size: 398 274 147 411 4234
Fragments arranged by size:
4234 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5239
Fragments arranged by size:
5239 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2701 1186
Size: 1515 3949
BseYI C'CCAG_C
Cuts at: 2172 2196 2220 2244 2268 2357 2392 4667 5163
Size: 24 24 24 24 89 35 2275 496
Cuts at: 5163 2172
Size: 2473
Fragments arranged by size:
2473 2275 496 89 35 24 24 24
24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4891
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2914 3675 4703 1540
Size: 275 186 913 761 1028 2301
Fragments arranged by size:
2301 1028 913 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2535 3525 3610 4681 2535
Size: 990 85 1071 3318
Fragments arranged by size:
3318 1071 990 85
BsmI GAATG_Cn'
Cuts at: 2975 3068 2975
Size: 93 5371
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2535 2783 3525 3610 4681 1391
Size: 399 745 248 742 85 1071 2174
Fragments arranged by size:
2174 1071 745 742 399 248 85
BspEI T'CCGG_A
Cuts at: 5127 5127
Size: 5464
BspHI T'CATG_A
Cuts at: 3353 5083 3353
Size: 1730 3734
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2819 844
Size: 1975 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3351 1394
Size: 429 1528 3507
Fragments arranged by size:
3507 1528 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3959 4133 65
Size: 1183 2711 174 1396
Fragments arranged by size:
2711 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4108 4295 1700
Size: 110 2298 187 2869
Fragments arranged by size:
2869 2298 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5464
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5464
BssSI C'ACGA_G
Cuts at: 3524 4536 3524
Size: 1012 4452
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5464
BstBI TT'CG_AA
Cuts at: 4268 4268
Size: 5464
BstEII G'GTnAC_C
Cuts at: 1184 2569 1184
Size: 1385 4079
BstXI CCAn_nnnn'nTGG
Cuts at: 2240 2240
Size: 5464
BstYI r'GATC_y
Cuts at: 1972 2794 3161 3546 3563 5015 5101 5113 5459
Size: 822 367 385 17 1452 86 12 346
Cuts at: 5459 1972
Size: 1977
Fragments arranged by size:
1977 1452 822 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2157 2157
Size: 5464
BtgI C'CryG_G
Cuts at: 513 1065 2004 2233 2499 513
Size: 552 939 229 266 3478
Fragments arranged by size:
3478 939 552 266 229
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4725
Fragments arranged by size:
4725 683 56
BtsI GCAGTG_nn'
Cuts at: 2370 2437 2980 3774 3794 2370
Size: 67 543 794 20 4040
Fragments arranged by size:
4040 794 543 67 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5464
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5429
DraI TTT'AAA
Cuts at: 2768 2768
Size: 5464
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4471 809
Size: 507 3155 1802
Fragments arranged by size:
3155 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2043 2911 3800
Size: 54 1425 50 275 18 213 868 889
Cuts at: 3800 5429 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 868 275 213 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2911 1537
Size: 275 1099 4090
Fragments arranged by size:
4090 1099 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2711 3394 1353
Size: 1358 683 3423
Fragments arranged by size:
3423 1358 683
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4051 4425 4571 387
Size: 1064 138 2462 374 146 1280
Fragments arranged by size:
2462 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2109 2157 3527 4124 4911 5179 1239
Size: 393 477 48 1370 597 787 268 1524
Fragments arranged by size:
1524 1370 787 597 477 393 268 48
EcoO109I rG'GnC_Cy
Cuts at: 2374 2374
Size: 5464
EcoRI G'AATT_C
Cuts at: 2773 3151 2773
Size: 378 5086
FspI TGC'GCA
Cuts at: 3970 3970
Size: 5464
HaeII r_GCGC'y
Cuts at: 1678 2080 4611 1678
Size: 402 2531 2531
Fragments arranged by size:
2531 2531 402
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2651 2683 4105 4137 4179
Size: 32 135 32 1079 32 1422 32 42
Cuts at: 4179 4211 1373
Size: 32 2626
Fragments arranged by size:
2626 1422 1079 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2808 3054 669
Size: 988 281 870 246 3079
Fragments arranged by size:
3079 988 870 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5464
HpaI GTT'AAC
Cuts at: 3054 3054
Size: 5464
KpnI G_GTAC'C
Cuts at: 2789 2789
Size: 5464
MfeI C'AATT_G
Cuts at: 3063 3063
Size: 5464
MluI A'CGCG_T
Cuts at: 5340 5340
Size: 5464
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4578 4762 1305
Size: 3273 184 2007
Fragments arranged by size:
3273 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2045 10
Size: 54 1425 343 213 3429
Fragments arranged by size:
3429 1425 343 213 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2190 2214 2238 2262 3422 3781 3940
Size: 648 1024 24 24 24 1160 359 159
Cuts at: 3940 518
Size: 2042
Fragments arranged by size:
2042 1160 1024 648 359 159 24 24
24
MspA1I CmG'CkG
Cuts at: 1606 2357 2880 3557 4705 4950 1606
Size: 751 523 677 1148 245 2120
Fragments arranged by size:
2120 1148 751 677 523 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2233 2499 513
Size: 552 939 229 266 3478
Fragments arranged by size:
3478 939 552 266 229
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5464
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5464
NotI GC'GGCC_GC
Cuts at: 2911 2911
Size: 5464
NspI r_CATG'y
Cuts at: 1132 1229 2392 2467 2822 4367 1132
Size: 97 1163 75 355 1545 2229
Fragments arranged by size:
2229 1545 1163 355 97 75
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5464
PciI A'CATG_T
Cuts at: 4363 4363
Size: 5464
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5464
PmeI GTTT'AAAC
Cuts at: 2768 2768
Size: 5464
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3533 3565 5046 5078 3533
Size: 32 1481 32 3919
Fragments arranged by size:
3919 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5464
PsiI TTA'TAA
Cuts at: 3034 3034
Size: 5464
PstI C_TGCA'G
Cuts at: 830 2353 2816 830
Size: 1523 463 3478
Fragments arranged by size:
3478 1523 463
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5464
PvuII CAG'CTG
Cuts at: 2357 2357
Size: 5464
SalI G'TCGA_C
Cuts at: 1936 2806 1936
Size: 870 4594
SbfI CC_TGCA'GG
Cuts at: 2816 2816
Size: 5464
ScaI AGT'ACT
Cuts at: 3712 5424 3712
Size: 1712 3752
SfcI C'TryA_G
Cuts at: 826 1045 2349 2491 2812 3947 4628 4819 826
Size: 219 1304 142 321 1135 681 191 1471
Fragments arranged by size:
1471 1304 1135 681 321 219 191 142
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5464
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5464
SmaI CCC'GGG
Cuts at: 1872 2121 2791 1872
Size: 249 670 4545
Fragments arranged by size:
4545 670 249
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3568 4469 4731 820
Size: 197 926 1625 901 262 1553
Fragments arranged by size:
1625 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3255 493
Size: 2762 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5464
SphI G_CATG'C
Cuts at: 1132 2822 1132
Size: 1690 3774
SspI AAT'ATT
Cuts at: 5 52 3388 5
Size: 47 3336 2081
Fragments arranged by size:
3336 2081 47
StuI AGG'CCT
Cuts at: 1517 2675 1517
Size: 1158 4306
StyI C'CwwG_G
Cuts at: 513 1065 2004 2014 2233 2377 2499 2583 2903
Size: 552 939 10 219 144 122 84 320
Cuts at: 2903 513
Size: 3074
Fragments arranged by size:
3074 939 552 320 219 144 122 84
10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2303 2621 2666 3491 3508 3661 3846
Size: 1255 723 318 45 825 17 153 185
Cuts at: 3846 4291 325
Size: 445 1498
Fragments arranged by size:
1498 1255 825 723 445 318 185 153
45 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2487 2508 3710
Size: 274 80 33 51 1443 510 21 1202
Cuts at: 3710 5422 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1202 510 274 138 80 51
33 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2709 2741 5424 5456 2709
Size: 32 2683 32 2717
Fragments arranged by size:
2717 2683 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2540 1316
Size: 1224 4240
XbaI T'CTAG_A
Cuts at: 2800 2800
Size: 5464
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5464
XmaI C'CCGG_G
Cuts at: 1870 2119 2789 1870
Size: 249 670 4545
Fragments arranged by size:
4545 670 249
XmnI GAAnn'nnTTC
Cuts at: 3593 3593
Size: 5464
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3272 276
Size: 53 83 186 936 1738 2468
Fragments arranged by size:
2468 1738 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI