Restriction map for
pFN21AB3481
(Circular) (Six-base) MAPSORT of: pFN21AB3481.seq Check: 8427 from: 1 to: 7051
pFN21AB3481 7051 bp
With 182 enzymes: SgfI *
February 27, 2009 14:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2128 2128
Size: 7051
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4861 278
Size: 53 83 186 936 3325 2468
Fragments arranged by size:
3325 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4394 1937
Size: 2457 4594
Acc65I G'GTAC_C
Cuts at: 4372 4372
Size: 7051
AclI AA'CG_TT
Cuts at: 1563 2851 5178 5551 1563
Size: 1288 2327 373 3063
Fragments arranged by size:
3063 2327 1288 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2225 2631 5114 6498 6766 1632
Size: 593 406 2483 1384 268 1917
Fragments arranged by size:
2483 1917 1384 593 406 268
AfeI AGC'GCT
Cuts at: 4340 4340
Size: 7051
AflII C'TTAA_G
Cuts at: 820 1017 2599 820
Size: 197 1582 5272
Fragments arranged by size:
5272 1582 197
AflIII A'CryG_T
Cuts at: 1203 4318 5950 6927 1203
Size: 3115 1632 977 1327
Fragments arranged by size:
3115 1632 1327 977
AgeI A'CCGG_T
Cuts at: 5882 5882
Size: 7051
AhdI GACnn_n'nnGTC
Cuts at: 5780 5780
Size: 7051
AleI CACnn'nnGTG
Cuts at: 1166 2159 3374 1166
Size: 993 1215 4843
Fragments arranged by size:
4843 1215 993
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7051
AlwNI CAG_nnn'CTG
Cuts at: 3643 4163 4794 6366 3643
Size: 520 631 1572 4328
Fragments arranged by size:
4328 1572 631 520
ApaI G_GGCC'C
Cuts at: 2694 2694
Size: 7051
ApaLI G'TGCA_C
Cuts at: 3000 3332 5108 6264 3000
Size: 332 1776 1156 3787
Fragments arranged by size:
3787 1776 1156 332
ApoI r'AATT_y
Cuts at: 1493 3445 3554 3623 3777 3787 4199 4360 4586
Size: 1952 109 69 154 10 412 161 226
Cuts at: 4586 4738 6847 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1952 1697 412 226 161 154 152
109 69 10
AseI AT'TA_AT
Cuts at: 160 1032 5605 160
Size: 872 4573 1606
Fragments arranged by size:
4573 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7051
AvaI C'yCGr_G
Cuts at: 1870 1943 2092 2703 4376 1870
Size: 73 149 611 1673 4545
Fragments arranged by size:
4545 1673 611 149 73
BamHI G'GATC_C
Cuts at: 4381 4748 4381
Size: 367 6684
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2153 3537 4238 4306 4372
Size: 325 217 627 366 1384 701 68 66
Cuts at: 4372 5827 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1384 701 627 366 325 217
68 66
BanII G_rGCy'C
Cuts at: 1391 2631 2694 4370 1391
Size: 1240 63 1676 4072
Fragments arranged by size:
4072 1676 1240 63
BbeI G_GCGC'C
Cuts at: 4310 4310
Size: 7051
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4107 928
Size: 971 2208 3872
Fragments arranged by size:
3872 2208 971
BbvCI CC'TCA_GC
Cuts at: 3528 3528
Size: 7051
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5242 5276 1903
Size: 34 3305 34 3678
Fragments arranged by size:
3678 3305 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4003 4943 6153 4003
Size: 940 1210 4901
Fragments arranged by size:
4901 1210 940
BclI T'GATC_A
Cuts at: 1548 3645 4071 1548
Size: 2097 426 4528
Fragments arranged by size:
4528 2097 426
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5662 136
Size: 107 122 71 631 605 3990 1525
Fragments arranged by size:
3990 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3168 3879 7046 3168
Size: 711 3167 3173
Fragments arranged by size:
3173 3167 711
BlpI GC'TnA_GC
Cuts at: 2432 4468 2432
Size: 2036 5015
Bme1580I G_kGCm'C
Cuts at: 1790 2694 3004 3336 3542 5112 6268 1790
Size: 904 310 332 206 1570 1156 2573
Fragments arranged by size:
2573 1570 1156 904 332 310 206
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7051
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3127 4782 5740
Size: 416 53 83 399 24 1710 1655 958
Cuts at: 5740 442
Size: 1753
Fragments arranged by size:
1753 1710 1655 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7051
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4306 4306
Size: 7051
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2206 2259 2463 2555 2828 2885 3767 3883
Size: 967 53 204 92 273 57 882 116
Cuts at: 3883 4192 5711 1239
Size: 309 1519 2579
Fragments arranged by size:
2579 1519 967 882 309 273 204 116
92 57 53
Bpu10I CC'TnA_GC
Cuts at: 3462 3528 3462
Size: 66 6985
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3278 5176 6041 6339 3278
Size: 1898 865 298 3990
Fragments arranged by size:
3990 1898 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2010 5714 882
Size: 617 511 3704 2219
Fragments arranged by size:
3704 2219 617 511
BsaAI yAC'GTr
Cuts at: 493 1204 4842 493
Size: 711 3638 2702
Fragments arranged by size:
3638 2702 711
BsaBI GATnn'nnATC
Cuts at: 7045 7045
Size: 7051
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2088 3904 4307 4858
Size: 53 83 186 936 555 1816 403 551
Cuts at: 4858 5240 5875 275
Size: 382 635 1451
Fragments arranged by size:
1816 1451 936 635 555 551 403 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 2895 5484 5882 6156 6303 6714 2895
Size: 2589 398 274 147 411 3232
Fragments arranged by size:
3232 2589 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2204 2234 1178
Size: 30 165 30 801 30 5995
Fragments arranged by size:
5995 801 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4109 4193 4196 1186
Size: 2923 84 3 4041
Fragments arranged by size:
4041 2923 84 3
BseYI C'CCAG_C
Cuts at: 3094 3241 3394 4193 4310 6254 6750 3094
Size: 147 153 799 117 1944 496 3395
Fragments arranged by size:
3395 1944 799 496 153 147 117
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3398 1163
Size: 573 1662 4816
Fragments arranged by size:
4816 1662 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4501 5262 6290 1540
Size: 275 186 2500 761 1028 2301
Fragments arranged by size:
2500 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2631 3004 3336 3369 5112 5197 6268 2631
Size: 373 332 33 1743 85 1071 3414
Fragments arranged by size:
3414 1743 1071 373 332 85 33
BsmI GAATG_Cn'
Cuts at: 2597 4562 4655 2597
Size: 1965 93 4993
Fragments arranged by size:
4993 1965 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3762 3762
Size: 7051
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2631 2694 3004 3336 3369 3542 4370
Size: 399 841 63 310 332 33 173 828
Cuts at: 4370 5112 5197 6268 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 841 828 742 399 332 310
173 85 63 33
BspEI T'CCGG_A
Cuts at: 2895 6714 2895
Size: 3819 3232
BspHI T'CATG_A
Cuts at: 3315 3655 4940 6670 3315
Size: 340 1285 1730 3696
Fragments arranged by size:
3696 1730 1285 340
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2128 4406 844
Size: 1284 2278 3489
Fragments arranged by size:
3489 2278 1284
BsrBI CCG'CTC
Cuts at: 1394 1823 2024 4330 4938 1394
Size: 429 201 2306 608 3507
Fragments arranged by size:
3507 2306 608 429 201
BsrDI GCAATG_nn'
Cuts at: 65 1248 5546 5720 65
Size: 1183 4298 174 1396
Fragments arranged by size:
4298 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3174 5695 5882 1700
Size: 110 1364 2521 187 2869
Fragments arranged by size:
2869 2521 1364 187 110
BsrGI T'GTAC_A
Cuts at: 96 3603 4275 4321 96
Size: 3507 672 46 2826
Fragments arranged by size:
3507 2826 672 46
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7051
BssSI C'ACGA_G
Cuts at: 5111 6123 5111
Size: 1012 6039
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7051
BstBI TT'CG_AA
Cuts at: 5855 5855
Size: 7051
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7051
BstXI CCAn_nnnn'nTGG
Cuts at: 3484 4168 3484
Size: 684 6367
BstYI r'GATC_y
Cuts at: 1972 2713 3168 3879 4211 4381 4748 5133 5150
Size: 741 455 711 332 170 367 385 17
Cuts at: 5150 6602 6688 6700 7046 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 741 711 455 385 367 346
332 170 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2106 2423 2106
Size: 317 6734
BtgI C'CryG_G
Cuts at: 513 1065 2004 2739 3889 513
Size: 552 939 735 1150 3675
Fragments arranged by size:
3675 1150 939 735 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3493 4278 487
Size: 683 56 2267 785 3260
Fragments arranged by size:
3260 2267 785 683 56
BtsI GCAGTG_nn'
Cuts at: 2689 3128 4567 5361 5381 2689
Size: 439 1439 794 20 4359
Fragments arranged by size:
4359 1439 794 439 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7051
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7016
DraI TTT'AAA
Cuts at: 3444 4355 3444
Size: 911 6140
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6058 809
Size: 507 4742 1802
Fragments arranged by size:
4742 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2248 4498 5387
Size: 54 1425 50 275 18 418 2250 889
Cuts at: 5387 7016 8
Size: 1629 43
Fragments arranged by size:
2250 1629 1425 889 418 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4498 1537
Size: 275 2686 4090
Fragments arranged by size:
4090 2686 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2064 2076 2787 2963 3957 4119 4337 4981
Size: 711 12 711 176 994 162 218 644
Cuts at: 4981 1353
Size: 3423
Fragments arranged by size:
3423 994 711 711 644 218 176 162
12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5638 6012 6158 387
Size: 1064 138 4049 374 146 1280
Fragments arranged by size:
4049 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2629 2629
Size: 7051
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2206 2225 2259 2463 2555 2631 2828
Size: 393 574 19 34 204 92 76 197
Cuts at: 2828 2885 3767 3883 4192 5114 5711 6498 6766
Size: 57 882 116 309 922 597 787 268
Cuts at: 6766 1239
Size: 1524
Fragments arranged by size:
1524 922 882 787 597 574 393 309
268 204 197 116 92 76 57 34
19
EcoO109I rG'GnC_Cy
Cuts at: 2226 2575 2691 3261 3310 2226
Size: 349 116 570 49 5967
Fragments arranged by size:
5967 570 349 116 49
EcoRI G'AATT_C
Cuts at: 4360 4738 4360
Size: 378 6673
EcoRV GAT'ATC
Cuts at: 2920 2920
Size: 7051
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2527 2527
Size: 7051
FspI TGC'GCA
Cuts at: 5557 5557
Size: 7051
HaeII r_GCGC'y
Cuts at: 1678 4234 4310 4342 6198 1678
Size: 2556 76 32 1856 2531
Fragments arranged by size:
2556 2531 1856 76 32
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2204 2236 5692 5724 5766
Size: 32 135 32 632 32 3456 32 42
Cuts at: 5766 5798 1373
Size: 32 2626
Fragments arranged by size:
3456 2626 632 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2911 4395 4641 669
Size: 988 281 973 1484 246 3079
Fragments arranged by size:
3079 1484 988 973 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7051
HpaI GTT'AAC
Cuts at: 4641 4641
Size: 7051
KasI G'GCGC_C
Cuts at: 4306 4306
Size: 7051
KpnI G_GTAC'C
Cuts at: 4376 4376
Size: 7051
MfeI C'AATT_G
Cuts at: 4650 4650
Size: 7051
MluI A'CGCG_T
Cuts at: 6927 6927
Size: 7051
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6165 6349 1305
Size: 4860 184 2007
Fragments arranged by size:
4860 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2250 10
Size: 54 1425 343 418 4811
Fragments arranged by size:
4811 1425 418 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2159 2343 2550 2738 3374 5009 5368
Size: 648 993 184 207 188 636 1635 359
Cuts at: 5368 5527 518
Size: 159 2042
Fragments arranged by size:
2042 1635 993 648 636 359 207 188
184 159
MspA1I CmG'CkG
Cuts at: 1606 2203 2381 2945 3107 4467 5144 6292 6537
Size: 597 178 564 162 1360 677 1148 245
Cuts at: 6537 1606
Size: 2120
Fragments arranged by size:
2120 1360 1148 677 597 564 245 178
162
NarI GG'CG_CC
Cuts at: 4307 4307
Size: 7051
NcoI C'CATG_G
Cuts at: 513 1065 2004 2739 3889 513
Size: 552 939 735 1150 3675
Fragments arranged by size:
3675 1150 939 735 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7051
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7051
NotI GC'GGCC_GC
Cuts at: 4498 4498
Size: 7051
NspI r_CATG'y
Cuts at: 1132 1229 3069 4322 4409 5954 1132
Size: 97 1840 1253 87 1545 2229
Fragments arranged by size:
2229 1840 1545 1253 97 87
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7051
PciI A'CATG_T
Cuts at: 4318 5950 4318
Size: 1632 5419
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7051
PfoI T'CCnGG_A
Cuts at: 2045 2571 3321 2045
Size: 526 750 5775
Fragments arranged by size:
5775 750 526
PmeI GTTT'AAAC
Cuts at: 4355 4355
Size: 7051
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5120 5152 6633 6665 5120
Size: 32 1481 32 5506
Fragments arranged by size:
5506 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2575 3261 2575
Size: 686 6365
PshAI GACnn'nnGTC
Cuts at: 1613 2444 3413 3754 1613
Size: 831 969 341 4910
Fragments arranged by size:
4910 969 831 341
PsiI TTA'TAA
Cuts at: 4621 4621
Size: 7051
PspOMI G'GGCC_C
Cuts at: 2690 2690
Size: 7051
PstI C_TGCA'G
Cuts at: 830 3495 4403 830
Size: 2665 908 3478
Fragments arranged by size:
3478 2665 908
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7051
PvuII CAG'CTG
Cuts at: 2381 2945 3107 2381
Size: 564 162 6325
Fragments arranged by size:
6325 564 162
RsrII CG'GwC_CG
Cuts at: 2026 2026
Size: 7051
SacI G_AGCT'C
Cuts at: 2631 2631
Size: 7051
SalI G'TCGA_C
Cuts at: 1936 4393 1936
Size: 2457 4594
SapI GCTCTTCn'nnn_
Cuts at: 2963 4119 4337 2963
Size: 1156 218 5677
Fragments arranged by size:
5677 1156 218
SbfI CC_TGCA'GG
Cuts at: 4403 4403
Size: 7051
ScaI AGT'ACT
Cuts at: 5299 7011 5299
Size: 1712 5339
SfcI C'TryA_G
Cuts at: 826 1045 2185 3491 4399 5534 6215 6406 826
Size: 219 1140 1306 908 1135 681 191 1471
Fragments arranged by size:
1471 1306 1140 1135 908 681 219 191
SfoI GGC'GCC
Cuts at: 4308 4308
Size: 7051
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7051
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7051
SmaI CCC'GGG
Cuts at: 1872 2094 4378 1872
Size: 222 2284 4545
Fragments arranged by size:
4545 2284 222
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2599 3293 5155 6056 6318 820
Size: 197 926 656 694 1862 901 262 1553
Fragments arranged by size:
1862 1553 926 901 694 656 262 197
SnaBI TAC'GTA
Cuts at: 493 4842 493
Size: 4349 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7051
SphI G_CATG'C
Cuts at: 1132 3069 4409 1132
Size: 1937 1340 3774
Fragments arranged by size:
3774 1937 1340
SspI AAT'ATT
Cuts at: 5 52 4975 5
Size: 47 4923 2081
Fragments arranged by size:
4923 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7051
StyI C'CwwG_G
Cuts at: 513 1065 2004 2178 2739 3192 3828 3889 4490
Size: 552 939 174 561 453 636 61 601
Cuts at: 4490 513
Size: 3074
Fragments arranged by size:
3074 939 636 601 561 552 453 174
61
SwaI ATTT'AAAT
Cuts at: 3444 3444
Size: 7051
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2034 2224 5078 5095 5248 5433 5878
Size: 1255 454 190 2854 17 153 185 445
Cuts at: 5878 325
Size: 1498
Fragments arranged by size:
2854 1498 1255 454 445 190 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2349 2480 3603
Size: 274 80 33 51 1443 372 131 1123
Cuts at: 3603 3966 4275 4321 5297 7009 96
Size: 363 309 46 976 1712 138
Fragments arranged by size:
1712 1443 1123 976 372 363 309 274
138 131 80 51 46 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7011 7043 7011
Size: 32 7019
Tth111I GACn'n_nGTC
Cuts at: 1316 2806 1316
Size: 1490 5561
XbaI T'CTAG_A
Cuts at: 4387 4387
Size: 7051
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7051
XmaI C'CCGG_G
Cuts at: 1870 2092 4376 1870
Size: 222 2284 4545
Fragments arranged by size:
4545 2284 222
XmnI GAAnn'nnTTC
Cuts at: 2115 5180 2115
Size: 3065 3986
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4859 276
Size: 53 83 186 936 3325 2468
Fragments arranged by size:
3325 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I DraIII
EcoNI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PmlI PspXI PsrI SacII SanDI SexAI SfiI SrfI
XcmI