Restriction map for
pFN21AB3480
(Circular) (Six-base) MAPSORT of: pFN21AB3480.seq Check: 2783 from: 1 to: 5653
pFN21AB3480 5653 bp
With 182 enzymes: SgfI *
October 20, 2009 10:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2794 3463 278
Size: 53 83 186 936 1258 669 2468
Fragments arranged by size:
2468 1258 936 669 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2996 1937
Size: 1059 4594
Acc65I G'GTAC_C
Cuts at: 2974 2974
Size: 5653
AclI AA'CG_TT
Cuts at: 1563 2952 3780 4153 1563
Size: 1389 828 373 3063
Fragments arranged by size:
3063 1389 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3716 5100 5368 1632
Size: 2084 1384 268 1917
Fragments arranged by size:
2084 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5456
AflIII A'CryG_T
Cuts at: 1203 2307 4552 5529 1203
Size: 1104 2245 977 1327
Fragments arranged by size:
2245 1327 1104 977
AgeI A'CCGG_T
Cuts at: 4484 4484
Size: 5653
AhdI GACnn_n'nnGTC
Cuts at: 4382 4382
Size: 5653
AleI CACnn'nnGTG
Cuts at: 1166 2547 1166
Size: 1381 4272
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5653
AlwNI CAG_nnn'CTG
Cuts at: 3396 4968 3396
Size: 1572 4081
ApaLI G'TGCA_C
Cuts at: 3710 4866 3710
Size: 1156 4497
ApoI r'AATT_y
Cuts at: 1493 2492 2962 3188 3340 5449 1493
Size: 999 470 226 152 2109 1697
Fragments arranged by size:
2109 1697 999 470 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4207 160
Size: 872 3175 1606
Fragments arranged by size:
3175 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5653
AvaI C'yCGr_G
Cuts at: 1870 1943 2978 1870
Size: 73 1035 4545
Fragments arranged by size:
4545 1035 73
BamHI G'GATC_C
Cuts at: 2506 2983 3350 2506
Size: 477 367 4809
Fragments arranged by size:
4809 477 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2427 2884 2974 4429 618
Size: 325 217 627 640 457 90 1455 1842
Fragments arranged by size:
1842 1455 640 627 457 325 217 90
BanII G_rGCy'C
Cuts at: 1391 2142 2197 2972 1391
Size: 751 55 775 4072
Fragments arranged by size:
4072 775 751 55
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4682
BbvCI CC'TCA_GC
Cuts at: 2333 2423 2333
Size: 90 5563
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3844 3878 1903
Size: 34 1907 34 3678
Fragments arranged by size:
3678 1907 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3545 4755 3545
Size: 1210 4443
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5653
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2319 4264 136
Size: 107 122 71 631 605 647 1945 1525
Fragments arranged by size:
1945 1525 647 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5648 5648
Size: 5653
BlpI GC'TnA_GC
Cuts at: 3070 3070
Size: 5653
Bme1580I G_kGCm'C
Cuts at: 1790 2625 2887 3714 4870 1790
Size: 835 262 827 1156 2573
Fragments arranged by size:
2573 1156 835 827 262
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5653
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3384 4342 442
Size: 416 53 83 399 24 1967 958 1753
Fragments arranged by size:
1967 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5653
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2230 2230
Size: 5653
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2144 2363 2414 4313 1239
Size: 905 219 51 1899 2579
Fragments arranged by size:
2579 1899 905 219 51
Bpu10I CC'TnA_GC
Cuts at: 2333 2423 2333
Size: 90 5563
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2156 3778 4643 4941 2156
Size: 1622 865 298 2868
Fragments arranged by size:
2868 1622 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2009 4316 882
Size: 617 510 2307 2219
Fragments arranged by size:
2307 2219 617 510
BsaAI yAC'GTr
Cuts at: 493 1204 2310 3444 493
Size: 711 1106 1134 2702
Fragments arranged by size:
2702 1134 1106 711
BsaBI GATnn'nnATC
Cuts at: 2804 5647 2804
Size: 2843 2810
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2791 3460 3842 4477
Size: 53 83 186 936 1258 669 382 635
Cuts at: 4477 275
Size: 1451
Fragments arranged by size:
1451 1258 936 669 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4086 4484 4758 4905 5316 4086
Size: 398 274 147 411 4423
Fragments arranged by size:
4423 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2294 2324 2908 2938 1178
Size: 30 165 30 891 30 584 30 3893
Fragments arranged by size:
3893 891 584 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5653
BseYI C'CCAG_C
Cuts at: 2264 2624 4856 5352 2264
Size: 360 2232 496 2565
Fragments arranged by size:
2565 2232 496 360
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2545 1163
Size: 573 809 4271
Fragments arranged by size:
4271 809 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3103 3864 4892 1540
Size: 275 186 1102 761 1028 2301
Fragments arranged by size:
2301 1102 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2160 2379 3714 3799 4870 2160
Size: 219 1335 85 1071 2943
Fragments arranged by size:
2943 1335 1071 219 85
BsmI GAATG_Cn'
Cuts at: 3164 3257 3164
Size: 93 5560
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2142 2160 2197 2379 2625 2887 2972
Size: 399 352 18 37 182 246 262 85
Cuts at: 2972 3714 3799 4870 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 352 262 246 182
85 85 37 18
BspEI T'CCGG_A
Cuts at: 5316 5316
Size: 5653
BspHI T'CATG_A
Cuts at: 3542 5272 3542
Size: 1730 3923
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3008 844
Size: 2164 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3540 1394
Size: 429 1717 3507
Fragments arranged by size:
3507 1717 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2670 4148 4322 65
Size: 1183 1422 1478 174 1396
Fragments arranged by size:
1478 1422 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4297 4484 1700
Size: 110 2487 187 2869
Fragments arranged by size:
2869 2487 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5653
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5653
BssSI C'ACGA_G
Cuts at: 3713 4725 3713
Size: 1012 4641
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5653
BstBI TT'CG_AA
Cuts at: 4457 4457
Size: 5653
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5653
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5653
BstYI r'GATC_y
Cuts at: 1972 2506 2983 3350 3735 3752 5204 5290 5302
Size: 534 477 367 385 17 1452 86 12
Cuts at: 5302 5648 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 534 477 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2144 513
Size: 552 1079 4022
Fragments arranged by size:
4022 1079 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4914
Fragments arranged by size:
4914 683 56
BtsI GCAGTG_nn'
Cuts at: 2557 3169 3963 3983 2557
Size: 612 794 20 4227
Fragments arranged by size:
4227 794 612 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5653
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5618
DraI TTT'AAA
Cuts at: 2957 2957
Size: 5653
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2023 4660 809
Size: 507 707 2637 1802
Fragments arranged by size:
2637 1802 707 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2320 3100 3989
Size: 54 1425 50 275 18 490 780 889
Cuts at: 3989 5618 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 780 490 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3100 1537
Size: 275 1288 4090
Fragments arranged by size:
4090 1288 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2644 2925 3583 1353
Size: 1291 281 658 3423
Fragments arranged by size:
3423 1291 658 281
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4240 4614 4760 387
Size: 1064 138 2651 374 146 1280
Fragments arranged by size:
2651 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2144 2363 2414 3716 4313 5100 5368
Size: 393 512 219 51 1302 597 787 268
Cuts at: 5368 1239
Size: 1524
Fragments arranged by size:
1524 1302 787 597 512 393 268 219
51
EcoRI G'AATT_C
Cuts at: 2962 3340 2962
Size: 378 5275
FspI TGC'GCA
Cuts at: 4159 4159
Size: 5653
HaeII r_GCGC'y
Cuts at: 1678 4800 1678
Size: 3122 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2400 2432 2908 2940 4294
Size: 32 135 32 828 32 476 32 1354
Cuts at: 4294 4326 4368 4400 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1354 828 476 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2941 2997 3243 669
Size: 988 281 1003 56 246 3079
Fragments arranged by size:
3079 1003 988 281 246 56
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5653
HpaI GTT'AAC
Cuts at: 3243 3243
Size: 5653
KpnI G_GTAC'C
Cuts at: 2978 2978
Size: 5653
MfeI C'AATT_G
Cuts at: 3252 3252
Size: 5653
MluI A'CGCG_T
Cuts at: 5529 5529
Size: 5653
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4767 4951 1305
Size: 3462 184 2007
Fragments arranged by size:
3462 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2322 10
Size: 54 1425 343 490 3341
Fragments arranged by size:
3341 1425 490 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2547 3611 3970 4129 518
Size: 648 843 538 1064 359 159 2042
Fragments arranged by size:
2042 1064 843 648 538 359 159
MspA1I CmG'CkG
Cuts at: 1606 2298 2436 2852 3069 3746 4894 5139 1606
Size: 692 138 416 217 677 1148 245 2120
Fragments arranged by size:
2120 1148 692 677 416 245 217 138
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5101
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5653
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5653
NotI GC'GGCC_GC
Cuts at: 3100 3100
Size: 5653
NspI r_CATG'y
Cuts at: 1132 1229 2187 3011 4556 1132
Size: 97 958 824 1545 2229
Fragments arranged by size:
2229 1545 958 824 97
PasI CC'CwG_GG
Cuts at: 1348 2880 1348
Size: 1532 4121
PciI A'CATG_T
Cuts at: 4552 4552
Size: 5653
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2223 1108
Size: 1115 4538
PfoI T'CCnGG_A
Cuts at: 2160 2400 2160
Size: 240 5413
PmeI GTTT'AAAC
Cuts at: 2957 2957
Size: 5653
PmlI CAC'GTG
Cuts at: 2310 2310
Size: 5653
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3722 3754 5235 5267 3722
Size: 32 1481 32 4108
Fragments arranged by size:
4108 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5653
PsiI TTA'TAA
Cuts at: 3223 3223
Size: 5653
PstI C_TGCA'G
Cuts at: 830 2098 2726 3005 830
Size: 1268 628 279 3478
Fragments arranged by size:
3478 1268 628 279
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5653
PvuII CAG'CTG
Cuts at: 2436 2852 2436
Size: 416 5237
SalI G'TCGA_C
Cuts at: 1936 2995 1936
Size: 1059 4594
SapI GCTCTTCn'nnn_
Cuts at: 2644 2644
Size: 5653
SbfI CC_TGCA'GG
Cuts at: 3005 3005
Size: 5653
ScaI AGT'ACT
Cuts at: 3901 5613 3901
Size: 1712 3941
SfcI C'TryA_G
Cuts at: 826 1045 2094 2269 2682 2703 2722 3001 4136
Size: 219 1049 175 413 21 19 279 1135
Cuts at: 4136 4817 5008 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1049 681 413 279 219 191
175 21 19
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5653
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5653
SmaI CCC'GGG
Cuts at: 1872 2980 1872
Size: 1108 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2135 3757 4658 4920 820
Size: 197 926 192 1622 901 262 1553
Fragments arranged by size:
1622 1553 926 901 262 197 192
SnaBI TAC'GTA
Cuts at: 493 3444 493
Size: 2951 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5653
SphI G_CATG'C
Cuts at: 1132 3011 1132
Size: 1879 3774
SspI AAT'ATT
Cuts at: 5 52 3577 5
Size: 47 3525 2081
Fragments arranged by size:
3525 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5653
StyI C'CwwG_G
Cuts at: 513 1065 3092 513
Size: 552 2027 3074
Fragments arranged by size:
3074 2027 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2267 3680 3697 3850 4035 4480 325
Size: 1255 687 1413 17 153 185 445 1498
Fragments arranged by size:
1498 1413 1255 687 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2304 3899 5611
Size: 274 80 33 51 1443 327 1595 1712
Cuts at: 5611 96
Size: 138
Fragments arranged by size:
1712 1595 1443 327 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2294 2326 5613 5645 2294
Size: 32 3287 32 2302
Fragments arranged by size:
3287 2302 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2936 1316
Size: 1620 4033
XbaI T'CTAG_A
Cuts at: 2989 2989
Size: 5653
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2543 2543
Size: 5653
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5653
XmaI C'CCGG_G
Cuts at: 1870 2978 1870
Size: 1108 4545
XmnI GAAnn'nnTTC
Cuts at: 3782 3782
Size: 5653
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2792 3461 276
Size: 53 83 186 936 1258 669 2468
Fragments arranged by size:
2468 1258 936 669 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI
PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI
SwaI