Restriction map for
pFN21AB3476
(Circular) (Six-base) MAPSORT of: pFN21AB3476.seq Check: 117 from: 1 to: 6874
pFN21AB3476 6874 bp
With 182 enzymes: SgfI *
February 27, 2009 14:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4684 278
Size: 53 83 186 936 3148 2468
Fragments arranged by size:
3148 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4217 1937
Size: 2280 4594
Acc65I G'GTAC_C
Cuts at: 3666 4195 3666
Size: 529 6345
AclI AA'CG_TT
Cuts at: 1563 5001 5374 1563
Size: 3438 373 3063
Fragments arranged by size:
3438 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2016 4937 6321 6589 1632
Size: 384 2921 1384 268 1917
Fragments arranged by size:
2921 1917 1384 384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6677
AflIII A'CryG_T
Cuts at: 1203 2171 2562 3189 5773 6750 1203
Size: 968 391 627 2584 977 1327
Fragments arranged by size:
2584 1327 977 968 627 391
AgeI A'CCGG_T
Cuts at: 5705 5705
Size: 6874
AhdI GACnn_n'nnGTC
Cuts at: 5603 5603
Size: 6874
AleI CACnn'nnGTG
Cuts at: 1166 2472 3675 1166
Size: 1306 1203 4365
Fragments arranged by size:
4365 1306 1203
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2490 1184
Size: 1306 5568
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3179 3211 3179
Size: 32 6842
AlwNI CAG_nnn'CTG
Cuts at: 4617 6189 4617
Size: 1572 5302
ApaLI G'TGCA_C
Cuts at: 4931 6087 4931
Size: 1156 5718
ApoI r'AATT_y
Cuts at: 1493 2842 2892 3077 3515 3578 3938 4169 4183
Size: 1349 50 185 438 63 360 231 14
Cuts at: 4183 4409 4561 6670 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1349 438 360 231 226 185
152 63 50 14
AseI AT'TA_AT
Cuts at: 160 1032 5428 160
Size: 872 4396 1606
Fragments arranged by size:
4396 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6874
AvaI C'yCGr_G
Cuts at: 1870 1943 3160 3363 4199 1870
Size: 73 1217 203 836 4545
Fragments arranged by size:
4545 1217 836 203 73
BamHI G'GATC_C
Cuts at: 3961 4204 4571 3961
Size: 243 367 6264
Fragments arranged by size:
6264 367 243
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3666 4195 5650 618
Size: 325 217 627 1879 529 1455 1842
Fragments arranged by size:
1879 1842 1455 627 529 325 217
BanII G_rGCy'C
Cuts at: 1391 4193 1391
Size: 2802 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5903
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5065 5099 1903
Size: 34 3128 34 3678
Fragments arranged by size:
3678 3128 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4766 5976 4766
Size: 1210 5664
BclI T'GATC_A
Cuts at: 1548 3265 1548
Size: 1717 5157
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3301 3847 5485
Size: 107 122 71 631 605 1629 546 1638
Cuts at: 5485 136
Size: 1525
Fragments arranged by size:
1638 1629 1525 631 605 546 122 107
71
BglII A'GATC_T
Cuts at: 2779 6869 2779
Size: 4090 2784
BlpI GC'TnA_GC
Cuts at: 4291 4291
Size: 6874
Bme1580I G_kGCm'C
Cuts at: 1790 4935 6091 1790
Size: 3145 1156 2573
Fragments arranged by size:
3145 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2174 1318
Size: 856 6018
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2866 4605 5563
Size: 416 53 83 399 24 1449 1739 958
Cuts at: 5563 442
Size: 1753
Fragments arranged by size:
1753 1739 1449 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 3308 1056
Size: 2252 4622
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3619 3619
Size: 6874
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2142 2630 2870 3363 3627 5534 1239
Size: 903 488 240 493 264 1907 2579
Fragments arranged by size:
2579 1907 903 493 488 264 240
Bpu10I CC'TnA_GC
Cuts at: 2451 2451
Size: 6874
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2198 2414 4999 5864 6162 2198
Size: 216 2585 865 298 2910
Fragments arranged by size:
2910 2585 865 298 216
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5537 882
Size: 617 4038 2219
Fragments arranged by size:
4038 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2853 3455 4665 493
Size: 711 1649 602 1210 2702
Fragments arranged by size:
2702 1649 1210 711 602
BsaBI GATnn'nnATC
Cuts at: 3809 3960 6868 3809
Size: 151 2908 3815
Fragments arranged by size:
3815 2908 151
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4681 5063 5698 275
Size: 53 83 186 936 3148 382 635 1451
Fragments arranged by size:
3148 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5307 5705 5979 6126 6537 5307
Size: 398 274 147 411 5644
Fragments arranged by size:
5644 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2603 2633 3179 3209 1178
Size: 30 165 30 1200 30 546 30 4843
Fragments arranged by size:
4843 1200 546 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2027 2066 1186
Size: 841 39 5994
Fragments arranged by size:
5994 841 39
BseYI C'CCAG_C
Cuts at: 6077 6573 6077
Size: 496 6378
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6301
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4324 5085 6113 1540
Size: 275 186 2323 761 1028 2301
Fragments arranged by size:
2323 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2479 3349 4935 5020 6091 2479
Size: 870 1586 85 1071 3262
Fragments arranged by size:
3262 1586 1071 870 85
BsiWI C'GTAC_G
Cuts at: 3455 3455
Size: 6874
BsmI GAATG_Cn'
Cuts at: 4385 4478 4385
Size: 93 6781
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2479 3349 4193 4935 5020 6091 1391
Size: 399 689 870 844 742 85 1071 2174
Fragments arranged by size:
2174 1071 870 844 742 689 399 85
BspEI T'CCGG_A
Cuts at: 6537 6537
Size: 6874
BspHI T'CATG_A
Cuts at: 3010 4763 6493 3010
Size: 1753 1730 3391
Fragments arranged by size:
3391 1753 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4229 844
Size: 3385 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4123 4761 1394
Size: 429 2300 638 3507
Fragments arranged by size:
3507 2300 638 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2599 2694 5369 5543 65
Size: 1183 1351 95 2675 174 1396
Fragments arranged by size:
2675 1396 1351 1183 174 95
BsrFI r'CCGG_y
Cuts at: 1700 1810 5518 5705 1700
Size: 110 3708 187 2869
Fragments arranged by size:
3708 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3971 96
Size: 3875 2999
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6874
BssSI C'ACGA_G
Cuts at: 4934 5946 4934
Size: 1012 5862
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6874
BstBI TT'CG_AA
Cuts at: 3991 5678 3991
Size: 1687 5187
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6874
BstXI CCAn_nnnn'nTGG
Cuts at: 3282 3691 3943 3282
Size: 409 252 6213
Fragments arranged by size:
6213 409 252
BstYI r'GATC_y
Cuts at: 1972 2117 2779 2914 3961 4204 4571 4956 4973
Size: 145 662 135 1047 243 367 385 17
Cuts at: 4973 6425 6511 6523 6869 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1047 662 385 367 346 243
145 135 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2496 3408 4090 513
Size: 552 1431 912 682 3297
Fragments arranged by size:
3297 1431 912 682 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6135
Fragments arranged by size:
6135 683 56
BtsI GCAGTG_nn'
Cuts at: 2463 3250 4390 5184 5204 2463
Size: 787 1140 794 20 4133
Fragments arranged by size:
4133 1140 794 787 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6874
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6839
DraI TTT'AAA
Cuts at: 2829 3487 4178 2829
Size: 658 691 5525
Fragments arranged by size:
5525 691 658
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4085 5881 809
Size: 507 2769 1796 1802
Fragments arranged by size:
2769 1802 1796 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2343 3272 4321
Size: 54 1425 50 275 18 513 929 1049
Cuts at: 4321 5210 6839 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1049 929 889 513 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4321 1537
Size: 275 2509 4090
Fragments arranged by size:
4090 2509 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2070 2608 4804 1353
Size: 717 538 2196 3423
Fragments arranged by size:
3423 2196 717 538
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2523 5461 5835 5981 387
Size: 1064 138 934 2938 374 146 1280
Fragments arranged by size:
2938 1280 1064 934 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2016 2142 2630 2870 3363 3627 4937
Size: 393 384 126 488 240 493 264 1310
Cuts at: 4937 5534 6321 6589 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1310 787 597 493 488 393 384
268 264 240 126
EcoO109I rG'GnC_Cy
Cuts at: 2386 2792 3279 2386
Size: 406 487 5981
Fragments arranged by size:
5981 487 406
EcoRI G'AATT_C
Cuts at: 3077 4183 4561 3077
Size: 1106 378 5390
Fragments arranged by size:
5390 1106 378
EcoRV GAT'ATC
Cuts at: 3529 3529
Size: 6874
FspI TGC'GCA
Cuts at: 5380 5380
Size: 6874
HaeII r_GCGC'y
Cuts at: 1678 6021 1678
Size: 4343 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2276 2308 3757 3789 5515
Size: 32 135 32 704 32 1449 32 1726
Cuts at: 5515 5547 5589 5621 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1726 1449 704 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4218 4464 669
Size: 988 281 2280 246 3079
Fragments arranged by size:
3079 2280 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2646 748
Size: 1898 4976
HpaI GTT'AAC
Cuts at: 4464 4464
Size: 6874
KpnI G_GTAC'C
Cuts at: 3670 4199 3670
Size: 529 6345
MfeI C'AATT_G
Cuts at: 4473 4473
Size: 6874
MluI A'CGCG_T
Cuts at: 6750 6750
Size: 6874
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2768 3403 5988 6172 1305
Size: 1463 635 2585 184 2007
Fragments arranged by size:
2585 2007 1463 635 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5052
Fragments arranged by size:
5052 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2472 3675 3741 4832 5191 5350 518
Size: 648 1306 1203 66 1091 359 159 2042
Fragments arranged by size:
2042 1306 1203 1091 648 359 159 66
MspA1I CmG'CkG
Cuts at: 1606 3691 4290 4967 6115 6360 1606
Size: 2085 599 677 1148 245 2120
Fragments arranged by size:
2120 2085 1148 677 599 245
NcoI C'CATG_G
Cuts at: 513 1065 2496 3408 513
Size: 552 1431 912 3979
Fragments arranged by size:
3979 1431 912 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6874
NheI G'CTAG_C
Cuts at: 1052 3304 1052
Size: 2252 4622
NotI GC'GGCC_GC
Cuts at: 4321 4321
Size: 6874
NspI r_CATG'y
Cuts at: 1132 1229 2566 3193 4232 5777 1132
Size: 97 1337 627 1039 1545 2229
Fragments arranged by size:
2229 1545 1337 1039 627 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6874
PciI A'CATG_T
Cuts at: 2562 3189 5773 2562
Size: 627 2584 3663
Fragments arranged by size:
3663 2584 627
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2856 3868 1108
Size: 1748 1012 4114
Fragments arranged by size:
4114 1748 1012
PfoI T'CCnGG_A
Cuts at: 3111 3111
Size: 6874
PmeI GTTT'AAAC
Cuts at: 4178 4178
Size: 6874
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3176 3179 3208 3211 4943 4975 6456 6488 3176
Size: 3 29 3 1732 32 1481 32 3562
Fragments arranged by size:
3562 1732 1481 32 32 29 3 3
PpuMI rG'GwC_Cy
Cuts at: 2386 2792 3279 2386
Size: 406 487 5981
Fragments arranged by size:
5981 487 406
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6874
PsiI TTA'TAA
Cuts at: 3063 4444 3063
Size: 1381 5493
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2690 2722 3858 3890 2690
Size: 32 1136 32 5674
Fragments arranged by size:
5674 1136 32 32
PstI C_TGCA'G
Cuts at: 830 4226 830
Size: 3396 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6874
PvuII CAG'CTG
Cuts at: 3691 3691
Size: 6874
RsrII CG'GwC_CG
Cuts at: 2531 2531
Size: 6874
SalI G'TCGA_C
Cuts at: 1936 4216 1936
Size: 2280 4594
SbfI CC_TGCA'GG
Cuts at: 4226 4226
Size: 6874
ScaI AGT'ACT
Cuts at: 5122 6834 5122
Size: 1712 5162
SfcI C'TryA_G
Cuts at: 826 1045 2758 3178 4127 4222 5357 6038 6229
Size: 219 1713 420 949 95 1135 681 191
Cuts at: 6229 826
Size: 1471
Fragments arranged by size:
1713 1471 1135 949 681 420 219 191
95
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6874
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6874
SmaI CCC'GGG
Cuts at: 1872 3365 4201 1872
Size: 1493 836 4545
Fragments arranged by size:
4545 1493 836
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2177 2429 4978 5879 6141 820
Size: 197 926 234 252 2549 901 262 1553
Fragments arranged by size:
2549 1553 926 901 262 252 234 197
SnaBI TAC'GTA
Cuts at: 493 3455 4665 493
Size: 2962 1210 2702
Fragments arranged by size:
2962 2702 1210
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6874
SphI G_CATG'C
Cuts at: 1132 4232 1132
Size: 3100 3774
SspI AAT'ATT
Cuts at: 5 52 4798 5
Size: 47 4746 2081
Fragments arranged by size:
4746 2081 47
StuI AGG'CCT
Cuts at: 1517 2340 1517
Size: 823 6051
StyI C'CwwG_G
Cuts at: 513 1065 2150 2418 2496 3275 3408 3520 4313
Size: 552 1085 268 78 779 133 112 793
Cuts at: 4313 513
Size: 3074
Fragments arranged by size:
3074 1085 793 779 552 268 133 112
78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2477 4901 4918 5071 5256 5701 325
Size: 1255 897 2424 17 153 185 445 1498
Fragments arranged by size:
2424 1498 1255 897 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2697 3971 5120
Size: 274 80 33 51 1443 720 1274 1149
Cuts at: 5120 6832 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1274 1149 720 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2603 2635 2828 2860 6834 6866 2603
Size: 32 193 32 3974 32 2611
Fragments arranged by size:
3974 2611 193 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6874
XbaI T'CTAG_A
Cuts at: 4210 4210
Size: 6874
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2894 2894
Size: 6874
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6874
XmaI C'CCGG_G
Cuts at: 1870 3363 4199 1870
Size: 1493 836 4545
Fragments arranged by size:
4545 1493 836
XmnI GAAnn'nnTTC
Cuts at: 3191 5003 3191
Size: 1812 5062
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4682 276
Size: 53 83 186 936 3148 2468
Fragments arranged by size:
3148 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PsrI PstI PvuI PvuII RsrII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI