Restriction map for
pFN21AB3473
(Circular) (Six-base) MAPSORT of: pFN21AB3473.seq Check: 1237 from: 1 to: 6196
pFN21AB3473 6196 bp
With 182 enzymes: SgfI *
February 27, 2009 14:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2603 2603
Size: 6196
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4006 278
Size: 53 83 186 936 2470 2468
Fragments arranged by size:
2470 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2210 3539 1937
Size: 273 1329 4594
Fragments arranged by size:
4594 1329 273
Acc65I G'GTAC_C
Cuts at: 3517 3517
Size: 6196
AclI AA'CG_TT
Cuts at: 1563 2516 4323 4696 1563
Size: 953 1807 373 3063
Fragments arranged by size:
3063 1807 953 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4259 5643 5911 1632
Size: 2627 1384 268 1917
Fragments arranged by size:
2627 1917 1384 268
AfeI AGC'GCT
Cuts at: 3007 3007
Size: 6196
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5999
AflIII A'CryG_T
Cuts at: 1203 3483 5095 6072 1203
Size: 2280 1612 977 1327
Fragments arranged by size:
2280 1612 1327 977
AgeI A'CCGG_T
Cuts at: 5027 5027
Size: 6196
AhdI GACnn_n'nnGTC
Cuts at: 4925 4925
Size: 6196
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6196
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2765 1184
Size: 1581 4615
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2866 2898 2866
Size: 32 6164
AlwNI CAG_nnn'CTG
Cuts at: 3939 5511 3939
Size: 1572 4624
ApaLI G'TGCA_C
Cuts at: 4253 5409 4253
Size: 1156 5040
ApoI r'AATT_y
Cuts at: 1493 2707 3505 3731 3883 5992 1493
Size: 1214 798 226 152 2109 1697
Fragments arranged by size:
2109 1697 1214 798 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2087 3156 4750 160
Size: 872 1055 1069 1594 1606
Fragments arranged by size:
1606 1594 1069 1055 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6196
AvaI C'yCGr_G
Cuts at: 1870 1943 3204 3521 1870
Size: 73 1261 317 4545
Fragments arranged by size:
4545 1261 317 73
AvrII C'CTAG_G
Cuts at: 3088 3088
Size: 6196
BamHI G'GATC_C
Cuts at: 3388 3526 3893 3388
Size: 138 367 5691
Fragments arranged by size:
5691 367 138
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3517 4972 618
Size: 325 217 627 1730 1455 1842
Fragments arranged by size:
1842 1730 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2300 2584 3515 1391
Size: 909 284 931 4072
Fragments arranged by size:
4072 931 909 284
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5225
BbvCI CC'TCA_GC
Cuts at: 3104 3104
Size: 6196
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4387 4421 1903
Size: 34 2450 34 3678
Fragments arranged by size:
3678 2450 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4088 5298 4088
Size: 1210 4986
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6196
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4807 136
Size: 107 122 71 631 605 3135 1525
Fragments arranged by size:
3135 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2812 6191 2812
Size: 3379 2817
BlpI GC'TnA_GC
Cuts at: 3613 3613
Size: 6196
Bme1580I G_kGCm'C
Cuts at: 1790 4257 5413 1790
Size: 2467 1156 2573
Fragments arranged by size:
2573 2467 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6196
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2906 3927 4885
Size: 416 53 83 399 24 1489 1021 958
Cuts at: 4885 442
Size: 1753
Fragments arranged by size:
1753 1489 1021 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2181 1056
Size: 1125 5071
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2598 3141 4856 1239
Size: 1359 543 1715 2579
Fragments arranged by size:
2579 1715 1359 543
Bpu10I CC'TnA_GC
Cuts at: 2737 3104 2737
Size: 367 5829
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2189 2677 2871 4321 5186 5484 2189
Size: 488 194 1450 865 298 2901
Fragments arranged by size:
2901 1450 865 488 298 194
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2584 4859 882
Size: 617 1085 2275 2219
Fragments arranged by size:
2275 2219 1085 617
BsaAI yAC'GTr
Cuts at: 493 1204 3987 493
Size: 711 2783 2702
Fragments arranged by size:
2783 2702 711
BsaBI GATnn'nnATC
Cuts at: 6190 6190
Size: 6196
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4003 4385 5020 275
Size: 53 83 186 936 2470 382 635 1451
Fragments arranged by size:
2470 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4629 5027 5301 5448 5859 4629
Size: 398 274 147 411 4966
Fragments arranged by size:
4966 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2746 2776 1178
Size: 30 165 30 1343 30 4598
Fragments arranged by size:
4598 1343 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2574 3192 1186
Size: 1388 618 4190
Fragments arranged by size:
4190 1388 618
BseYI C'CCAG_C
Cuts at: 3055 3285 3306 5399 5895 3055
Size: 230 21 2093 496 3356
Fragments arranged by size:
3356 2093 496 230 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5623
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3646 4407 5435 1540
Size: 275 186 1645 761 1028 2301
Fragments arranged by size:
2301 1645 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2118 2325 2584 2630 4257 4342 5413 2118
Size: 207 259 46 1627 85 1071 2901
Fragments arranged by size:
2901 1627 1071 259 207 85 46
BsmI GAATG_Cn'
Cuts at: 3051 3707 3800 3051
Size: 656 93 5447
Fragments arranged by size:
5447 656 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2118 2300 2325 2584 2630 3515 4257
Size: 399 328 182 25 259 46 885 742
Cuts at: 4257 4342 5413 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 885 742 399 328 259 182
85 46 25
BspEI T'CCGG_A
Cuts at: 5859 5859
Size: 6196
BspHI T'CATG_A
Cuts at: 2230 4085 5815 2230
Size: 1855 1730 2611
Fragments arranged by size:
2611 1855 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2603 3551 844
Size: 1759 948 3489
Fragments arranged by size:
3489 1759 948
BsrBI CCG'CTC
Cuts at: 1394 1823 4083 1394
Size: 429 2260 3507
Fragments arranged by size:
3507 2260 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2614 2973 4691 4865 65
Size: 1183 1366 359 1718 174 1396
Fragments arranged by size:
1718 1396 1366 1183 359 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4840 5027 1700
Size: 110 3030 187 2869
Fragments arranged by size:
3030 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6196
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6196
BssSI C'ACGA_G
Cuts at: 4256 5268 4256
Size: 1012 5184
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6196
BstBI TT'CG_AA
Cuts at: 5000 5000
Size: 6196
BstEII G'GTnAC_C
Cuts at: 1184 3490 1184
Size: 2306 3890
BstYI r'GATC_y
Cuts at: 1972 2812 3388 3526 3893 4278 4295 5747 5833
Size: 840 576 138 367 385 17 1452 86
Cuts at: 5833 5845 6191 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 840 576 385 367 346 138
86 17 12
BstZ17I GTA'TAC
Cuts at: 2211 2211
Size: 6196
BtgI C'CryG_G
Cuts at: 513 1065 2004 3392 513
Size: 552 939 1388 3317
Fragments arranged by size:
3317 1388 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5457
Fragments arranged by size:
5457 683 56
BtsI GCAGTG_nn'
Cuts at: 3382 3712 4506 4526 3382
Size: 330 794 20 5052
Fragments arranged by size:
5052 794 330 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6196
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2596 2631 529
Size: 35 2032 35 4094
Fragments arranged by size:
4094 2032 35 35
DraI TTT'AAA
Cuts at: 3500 3500
Size: 6196
DraIII CAC_nnn'GTG
Cuts at: 2777 2777
Size: 6196
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5203 809
Size: 507 3887 1802
Fragments arranged by size:
3887 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3424 3643 4532
Size: 54 1425 50 275 18 1594 219 889
Cuts at: 4532 6161 8
Size: 1629 43
Fragments arranged by size:
1629 1594 1425 889 275 219 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3643 1537
Size: 275 1831 4090
Fragments arranged by size:
4090 1831 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4126 1353
Size: 2773 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2026 4783 5157 5303 387
Size: 1064 138 437 2757 374 146 1280
Fragments arranged by size:
2757 1280 1064 437 374 146 138
EcoICRI GAG'CTC
Cuts at: 2582 2582
Size: 6196
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2598 3141 4259 4856 5643 5911 1239
Size: 393 966 543 1118 597 787 268 1524
Fragments arranged by size:
1524 1118 966 787 597 543 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2772 2946 2772
Size: 174 6022
EcoO109I rG'GnC_Cy
Cuts at: 2390 2390
Size: 6196
EcoRI G'AATT_C
Cuts at: 3505 3883 3505
Size: 378 5818
FspI TGC'GCA
Cuts at: 4702 4702
Size: 6196
HaeII r_GCGC'y
Cuts at: 1678 3009 3331 5343 1678
Size: 1331 322 2012 2531
Fragments arranged by size:
2531 2012 1331 322
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2811 2843 3184 3216 4837
Size: 32 135 32 1239 32 341 32 1621
Cuts at: 4837 4869 4911 4943 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1621 1239 341 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2520 3540 3786 669
Size: 988 281 582 1020 246 3079
Fragments arranged by size:
3079 1020 988 582 281 246
HindIII A'AGCT_T
Cuts at: 748 2935 748
Size: 2187 4009
HpaI GTT'AAC
Cuts at: 2520 3786 2520
Size: 1266 4930
KpnI G_GTAC'C
Cuts at: 3521 3521
Size: 6196
MfeI C'AATT_G
Cuts at: 3414 3795 3414
Size: 381 5815
MluI A'CGCG_T
Cuts at: 3483 6072 3483
Size: 2589 3607
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5310 5494 1305
Size: 4005 184 2007
Fragments arranged by size:
4005 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4374
Fragments arranged by size:
4374 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2186 2565 4154 4513 4672 518
Size: 648 1020 379 1589 359 159 2042
Fragments arranged by size:
2042 1589 1020 648 379 359 159
MspA1I CmG'CkG
Cuts at: 1606 3612 4289 5437 5682 1606
Size: 2006 677 1148 245 2120
Fragments arranged by size:
2120 2006 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4705
Fragments arranged by size:
4705 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6196
NheI G'CTAG_C
Cuts at: 1052 2177 1052
Size: 1125 5071
NotI GC'GGCC_GC
Cuts at: 3643 3643
Size: 6196
NspI r_CATG'y
Cuts at: 1132 1229 2536 3554 5099 1132
Size: 97 1307 1018 1545 2229
Fragments arranged by size:
2229 1545 1307 1018 97
PasI CC'CwG_GG
Cuts at: 1348 2474 1348
Size: 1126 5070
PciI A'CATG_T
Cuts at: 5095 5095
Size: 6196
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2603 2777 2780 1108
Size: 1495 174 3 4524
Fragments arranged by size:
4524 1495 174 3
PfoI T'CCnGG_A
Cuts at: 2575 2575
Size: 6196
PmeI GTTT'AAAC
Cuts at: 3500 3500
Size: 6196
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4265 4297 5778 5810 4265
Size: 32 1481 32 4651
Fragments arranged by size:
4651 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3064 1613
Size: 1451 4745
PsiI TTA'TAA
Cuts at: 3766 3766
Size: 6196
PstI C_TGCA'G
Cuts at: 830 3548 830
Size: 2718 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6196
SacI G_AGCT'C
Cuts at: 2584 2584
Size: 6196
SalI G'TCGA_C
Cuts at: 1936 3538 1936
Size: 1602 4594
SbfI CC_TGCA'GG
Cuts at: 3548 3548
Size: 6196
ScaI AGT'ACT
Cuts at: 2602 4444 6156 2602
Size: 1842 1712 2642
Fragments arranged by size:
2642 1842 1712
SexAI A'CCwGG_T
Cuts at: 2773 2773
Size: 6196
SfcI C'TryA_G
Cuts at: 826 1045 2631 3544 4679 5360 5551 826
Size: 219 1586 913 1135 681 191 1471
Fragments arranged by size:
1586 1471 1135 913 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6196
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6196
SmaI CCC'GGG
Cuts at: 1872 3523 1872
Size: 1651 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2204 2692 2886 4300 5201 5463
Size: 197 926 261 488 194 1414 901 262
Cuts at: 5463 820
Size: 1553
Fragments arranged by size:
1553 1414 926 901 488 262 261 197
194
SnaBI TAC'GTA
Cuts at: 493 3987 493
Size: 3494 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6196
SphI G_CATG'C
Cuts at: 1132 2536 3554 1132
Size: 1404 1018 3774
Fragments arranged by size:
3774 1404 1018
SspI AAT'ATT
Cuts at: 5 52 4120 5
Size: 47 4068 2081
Fragments arranged by size:
4068 2081 47
StuI AGG'CCT
Cuts at: 1517 2280 1517
Size: 763 5433
StyI C'CwwG_G
Cuts at: 513 1065 2004 3088 3635 513
Size: 552 939 1084 547 3074
Fragments arranged by size:
3074 1084 939 552 547
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2882 3478 4223 4240 4393 4578 5023
Size: 1255 1302 596 745 17 153 185 445
Cuts at: 5023 325
Size: 1498
Fragments arranged by size:
1498 1302 1255 745 596 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2600 2958 4442
Size: 274 80 33 51 1443 623 358 1484
Cuts at: 4442 6154 96
Size: 1712 138
Fragments arranged by size:
1712 1484 1443 623 358 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6156 6188 6156
Size: 32 6164
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6196
XbaI T'CTAG_A
Cuts at: 3532 3532
Size: 6196
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6196
XmaI C'CCGG_G
Cuts at: 1870 3521 1870
Size: 1651 4545
XmnI GAAnn'nnTTC
Cuts at: 4325 4325
Size: 6196
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4004 276
Size: 53 83 186 936 2470 2468
Fragments arranged by size:
2470 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
ApaI AscI BaeI BbeI BfrBI BplI BsiWI BsmBI
BstXI Bsu36I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacII SanDI SapI SfiI
SfoI SrfI SwaI XcmI