Restriction map for
pFN21AB3467
(Circular) (Six-base) MAPSORT of: pFN21AB3467.seq Check: 6660 from: 1 to: 6691
pFN21AB3467 6691 bp
With 182 enzymes: SgfI *
February 27, 2009 14:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2983 4501 278
Size: 53 83 186 936 1447 1518 2468
Fragments arranged by size:
2468 1518 1447 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4034 1937
Size: 2097 4594
Acc65I G'GTAC_C
Cuts at: 4012 4012
Size: 6691
AclI AA'CG_TT
Cuts at: 1563 3990 4818 5191 1563
Size: 2427 828 373 3063
Fragments arranged by size:
3063 2427 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2058 2242 3826 4754 6138 6406 1632
Size: 426 184 1584 928 1384 268 1917
Fragments arranged by size:
1917 1584 1384 928 426 268 184
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6494
AflIII A'CryG_T
Cuts at: 1203 3538 3604 5590 6567 1203
Size: 2335 66 1986 977 1327
Fragments arranged by size:
2335 1986 1327 977 66
AgeI A'CCGG_T
Cuts at: 5522 5522
Size: 6691
AhdI GACnn_n'nnGTC
Cuts at: 3286 5420 3286
Size: 2134 4557
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6691
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2646 1184
Size: 1462 5229
AlwNI CAG_nnn'CTG
Cuts at: 2382 2687 3178 4434 6006 2382
Size: 305 491 1256 1572 3067
Fragments arranged by size:
3067 1572 1256 491 305
ApaLI G'TGCA_C
Cuts at: 4748 5904 4748
Size: 1156 5535
ApoI r'AATT_y
Cuts at: 1493 2357 2867 4000 4226 4378 6487 1493
Size: 864 510 1133 226 152 2109 1697
Fragments arranged by size:
2109 1697 1133 864 510 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2478 5245 160
Size: 872 1446 2767 1606
Fragments arranged by size:
2767 1606 1446 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6691
AvaI C'yCGr_G
Cuts at: 1870 1943 2033 4016 1870
Size: 73 90 1983 4545
Fragments arranged by size:
4545 1983 90 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3391 3424 3391
Size: 33 6658
BamHI G'GATC_C
Cuts at: 4021 4388 4021
Size: 367 6324
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2787 2912 3797 3824 4012
Size: 325 217 627 1000 125 885 27 188
Cuts at: 4012 5467 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1000 885 627 325 217 188
125 27
BanII G_rGCy'C
Cuts at: 1391 4010 1391
Size: 2619 4072
BbeI G_GCGC'C
Cuts at: 2791 2791
Size: 6691
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5720
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4882 4916 1903
Size: 34 2945 34 3678
Fragments arranged by size:
3678 2945 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3392 4583 5793 3392
Size: 1191 1210 4290
Fragments arranged by size:
4290 1210 1191
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6691
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3873 5302 136
Size: 107 122 71 631 605 2201 1429 1525
Fragments arranged by size:
2201 1525 1429 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2847 6686 2847
Size: 3839 2852
BlpI GC'TnA_GC
Cuts at: 4108 4108
Size: 6691
Bme1580I G_kGCm'C
Cuts at: 1790 2677 4752 5908 1790
Size: 887 2075 1156 2573
Fragments arranged by size:
2573 2075 1156 887
BmgBI CAC'GTC
Cuts at: 1318 3605 1318
Size: 2287 4404
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2481 4422 5380
Size: 416 53 83 399 24 1064 1941 958
Cuts at: 5380 442
Size: 1753
Fragments arranged by size:
1941 1753 1064 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6691
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2481 2601 2958 3395 5351 1239
Size: 1242 120 357 437 1956 2579
Fragments arranged by size:
2579 1956 1242 437 357 120
Bpu10I CC'TnA_GC
Cuts at: 3469 3469
Size: 6691
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2740 4816 5681 5979 2740
Size: 2076 865 298 3452
Fragments arranged by size:
3452 2076 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3344 3417 5354 882
Size: 617 1845 73 1937 2219
Fragments arranged by size:
2219 1937 1845 617 73
BsaAI yAC'GTr
Cuts at: 493 1204 4482 493
Size: 711 3278 2702
Fragments arranged by size:
3278 2702 711
BsaBI GATnn'nnATC
Cuts at: 6685 6685
Size: 6691
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2788 2980 4498 4880
Size: 53 83 186 936 1255 192 1518 382
Cuts at: 4880 5515 275
Size: 635 1451
Fragments arranged by size:
1518 1451 1255 936 635 382 192 186
83 53
BsaWI w'CCGG_w
Cuts at: 5124 5522 5796 5943 6354 5124
Size: 398 274 147 411 5461
Fragments arranged by size:
5461 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2792 2822 3100 3130 1178
Size: 30 165 30 1389 30 278 30 4739
Fragments arranged by size:
4739 1389 278 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2115 1186
Size: 929 5762
BseYI C'CCAG_C
Cuts at: 5894 6390 5894
Size: 496 6195
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3384 1163
Size: 573 1648 4470
Fragments arranged by size:
4470 1648 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4141 4902 5930 1540
Size: 275 186 2140 761 1028 2301
Fragments arranged by size:
2301 2140 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4752 4837 5908 4752
Size: 85 1071 5535
Fragments arranged by size:
5535 1071 85
BsiWI C'GTAC_G
Cuts at: 3596 3596
Size: 6691
BsmI GAATG_Cn'
Cuts at: 4202 4295 4202
Size: 93 6598
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2677 4010 4752 4837 5908 1391
Size: 399 887 1333 742 85 1071 2174
Fragments arranged by size:
2174 1333 1071 887 742 399 85
BspEI T'CCGG_A
Cuts at: 6354 6354
Size: 6691
BspHI T'CATG_A
Cuts at: 4580 6310 4580
Size: 1730 4961
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4046 844
Size: 3202 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3468 4578 1394
Size: 429 1645 1110 3507
Fragments arranged by size:
3507 1645 1110 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5186 5360 65
Size: 1183 3938 174 1396
Fragments arranged by size:
3938 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5335 5522 1700
Size: 110 3525 187 2869
Fragments arranged by size:
3525 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3580 96
Size: 3484 3207
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6691
BssSI C'ACGA_G
Cuts at: 3183 4751 5763 3183
Size: 1568 1012 4111
Fragments arranged by size:
4111 1568 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6691
BstBI TT'CG_AA
Cuts at: 5495 5495
Size: 6691
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6691
BstYI r'GATC_y
Cuts at: 1972 2222 2847 3676 4021 4388 4773 4790 6242
Size: 250 625 829 345 367 385 17 1452
Cuts at: 6242 6328 6340 6686 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 829 625 385 367 346 345
250 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3162 3162
Size: 6691
BtgI C'CryG_G
Cuts at: 513 1065 2332 3418 3886 513
Size: 552 1267 1086 468 3318
Fragments arranged by size:
3318 1267 1086 552 468
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5952
Fragments arranged by size:
5952 683 56
BtsI GCAGTG_nn'
Cuts at: 2025 3877 4207 5001 5021 2025
Size: 1852 330 794 20 3695
Fragments arranged by size:
3695 1852 794 330 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6691
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3471 3506 529
Size: 35 2907 35 3714
Fragments arranged by size:
3714 2907 35 35
DraI TTT'AAA
Cuts at: 3995 3995
Size: 6691
DraIII CAC_nnn'GTG
Cuts at: 2432 2432
Size: 6691
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5698 809
Size: 507 4382 1802
Fragments arranged by size:
4382 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3883 4138 5027
Size: 54 1425 50 275 18 2053 255 889
Cuts at: 5027 6656 8
Size: 1629 43
Fragments arranged by size:
2053 1629 1425 889 275 255 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4138 1537
Size: 275 2326 4090
Fragments arranged by size:
4090 2326 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2077 3217 3441 4621 1353
Size: 724 1140 224 1180 3423
Fragments arranged by size:
3423 1180 1140 724 224
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3119 3891 5278 5652 5798 387
Size: 1064 138 1530 772 1387 374 146 1280
Fragments arranged by size:
1530 1387 1280 1064 772 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2058 2242 2481 2601 2958 3395 3826
Size: 393 426 184 239 120 357 437 431
Cuts at: 3826 4754 5351 6138 6406 1239
Size: 928 597 787 268 1524
Fragments arranged by size:
1524 928 787 597 437 431 426 393
357 268 239 184 120
EcoO109I rG'GnC_Cy
Cuts at: 3451 3451
Size: 6691
EcoRI G'AATT_C
Cuts at: 4000 4378 4000
Size: 378 6313
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2269 3470 2269
Size: 1201 5490
FspI TGC'GCA
Cuts at: 5197 5197
Size: 6691
HaeII r_GCGC'y
Cuts at: 1678 2116 2791 5838 1678
Size: 438 675 3047 2531
Fragments arranged by size:
3047 2531 675 438
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2054 2086 2396 2428 2559
Size: 32 135 32 482 32 310 32 131
Cuts at: 2559 2591 3166 3198 3272 3304 5332 5364 5406
Size: 32 575 32 74 32 2028 32 42
Cuts at: 5406 5438 1373
Size: 32 2626
Fragments arranged by size:
2626 2028 575 482 310 135 131 74
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3976 4035 4281 669
Size: 988 281 2038 59 246 3079
Fragments arranged by size:
3079 2038 988 281 246 59
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6691
HpaI GTT'AAC
Cuts at: 4281 4281
Size: 6691
KasI G'GCGC_C
Cuts at: 2787 2787
Size: 6691
KpnI G_GTAC'C
Cuts at: 4016 4016
Size: 6691
MfeI C'AATT_G
Cuts at: 4290 4290
Size: 6691
MluI A'CGCG_T
Cuts at: 6567 6567
Size: 6691
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3042 3654 5805 5989 1305
Size: 1737 612 2151 184 2007
Fragments arranged by size:
2151 2007 1737 612 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3885 10
Size: 54 1425 343 2053 2816
Fragments arranged by size:
2816 2053 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3140 4649 5008 5167 518
Size: 648 1974 1509 359 159 2042
Fragments arranged by size:
2042 1974 1509 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4107 4784 5932 6177 1606
Size: 2501 677 1148 245 2120
Fragments arranged by size:
2501 2120 1148 677 245
NarI GG'CG_CC
Cuts at: 2788 2788
Size: 6691
NcoI C'CATG_G
Cuts at: 513 1065 3418 513
Size: 552 2353 3786
Fragments arranged by size:
3786 2353 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6691
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6691
NotI GC'GGCC_GC
Cuts at: 4138 4138
Size: 6691
NspI r_CATG'y
Cuts at: 1132 1229 2633 3408 3542 4049 5594 1132
Size: 97 1404 775 134 507 1545 2229
Fragments arranged by size:
2229 1545 1404 775 507 134 97
PasI CC'CwG_GG
Cuts at: 1348 3967 1348
Size: 2619 4072
PciI A'CATG_T
Cuts at: 3538 5590 3538
Size: 2052 4639
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6691
PfoI T'CCnGG_A
Cuts at: 2974 2974
Size: 6691
PmeI GTTT'AAAC
Cuts at: 3995 3995
Size: 6691
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2436 2468 4760 4792 6273 6305 2436
Size: 32 2292 32 1481 32 2822
Fragments arranged by size:
2822 2292 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6691
PsiI TTA'TAA
Cuts at: 3960 4261 3960
Size: 301 6390
PstI C_TGCA'G
Cuts at: 830 2701 4043 830
Size: 1871 1342 3478
Fragments arranged by size:
3478 1871 1342
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6691
SalI G'TCGA_C
Cuts at: 1936 4033 1936
Size: 2097 4594
SbfI CC_TGCA'GG
Cuts at: 4043 4043
Size: 6691
ScaI AGT'ACT
Cuts at: 4939 6651 4939
Size: 1712 4979
SfcI C'TryA_G
Cuts at: 826 1045 2697 3216 3495 4039 5174 5855 6046
Size: 219 1652 519 279 544 1135 681 191
Cuts at: 6046 826
Size: 1471
Fragments arranged by size:
1652 1471 1135 681 544 519 279 219
191
SfoI GGC'GCC
Cuts at: 2789 2789
Size: 6691
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6691
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6691
SmaI CCC'GGG
Cuts at: 1872 4018 1872
Size: 2146 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2719 4795 5696 5958 820
Size: 197 926 776 2076 901 262 1553
Fragments arranged by size:
2076 1553 926 901 776 262 197
SnaBI TAC'GTA
Cuts at: 493 4482 493
Size: 3989 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6691
SphI G_CATG'C
Cuts at: 1132 2633 4049 1132
Size: 1501 1416 3774
Fragments arranged by size:
3774 1501 1416
SspI AAT'ATT
Cuts at: 5 52 3658 4615 5
Size: 47 3606 957 2081
Fragments arranged by size:
3606 2081 957 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6691
StyI C'CwwG_G
Cuts at: 513 1065 2811 3418 3616 4130 513
Size: 552 1746 607 198 514 3074
Fragments arranged by size:
3074 1746 607 552 514 198
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3256 4718 4735 4888 5073 5518 325
Size: 1255 1676 1462 17 153 185 445 1498
Fragments arranged by size:
1676 1498 1462 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2096 2842 3580
Size: 274 80 33 51 1443 119 746 738
Cuts at: 3580 4937 6649 96
Size: 1357 1712 138
Fragments arranged by size:
1712 1443 1357 746 738 274 138 119
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2970 3002 6651 6683 2970
Size: 32 3649 32 2978
Fragments arranged by size:
3649 2978 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6691
XbaI T'CTAG_A
Cuts at: 2882 4027 2882
Size: 1145 5546
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2051 2684 2011
Size: 40 633 6018
Fragments arranged by size:
6018 633 40
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6691
XmaI C'CCGG_G
Cuts at: 1870 4016 1870
Size: 2146 4545
XmnI GAAnn'nnTTC
Cuts at: 2869 4820 2869
Size: 1951 4740
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2981 4499 276
Size: 53 83 186 936 1447 1518 2468
Fragments arranged by size:
2468 1518 1447 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbvCI BfrBI
BplI BsmBI BstXI BstZ17I EcoICRI EcoNI EcoRV FseI
FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SrfI SwaI