Restriction map for
pFN21AB3466
(Circular) (Six-base) MAPSORT of: pFN21AB3466.seq Check: 5162 from: 1 to: 6436
pFN21AB3466 6436 bp
With 182 enzymes: SgfI *
October 20, 2009 10:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4246 278
Size: 53 83 186 936 2710 2468
Fragments arranged by size:
2710 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3779 1937
Size: 1842 4594
Acc65I G'GTAC_C
Cuts at: 3757 3757
Size: 6436
AclI AA'CG_TT
Cuts at: 1563 4563 4936 1563
Size: 3000 373 3063
Fragments arranged by size:
3063 3000 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2600 2605 3077 3213 3413 3590 4499 5883
Size: 968 5 472 136 200 177 909 1384
Cuts at: 5883 6151 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 968 909 472 268 200 177
136 5
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6239
AflIII A'CryG_T
Cuts at: 1203 5335 6312 1203
Size: 4132 977 1327
Fragments arranged by size:
4132 1327 977
AgeI A'CCGG_T
Cuts at: 5267 5267
Size: 6436
AhdI GACnn_n'nnGTC
Cuts at: 5165 5165
Size: 6436
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6436
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6436
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2117 2149 2117
Size: 32 6404
AlwNI CAG_nnn'CTG
Cuts at: 2305 3428 4179 5751 2305
Size: 1123 751 1572 2990
Fragments arranged by size:
2990 1572 1123 751
ApaLI G'TGCA_C
Cuts at: 4493 5649 4493
Size: 1156 5280
ApoI r'AATT_y
Cuts at: 1493 2765 3722 3745 3971 4123 6232 1493
Size: 1272 957 23 226 152 2109 1697
Fragments arranged by size:
2109 1697 1272 957 226 152 23
AseI AT'TA_AT
Cuts at: 160 1032 4990 160
Size: 872 3958 1606
Fragments arranged by size:
3958 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6436
AvaI C'yCGr_G
Cuts at: 1870 1943 2931 3225 3761 1870
Size: 73 988 294 536 4545
Fragments arranged by size:
4545 988 536 294 73
BamHI G'GATC_C
Cuts at: 2517 3766 4133 2517
Size: 1249 367 4820
Fragments arranged by size:
4820 1249 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3757 5212 618
Size: 325 217 627 1970 1455 1842
Fragments arranged by size:
1970 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3121 3755 1391
Size: 1730 634 4072
Fragments arranged by size:
4072 1730 634
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2800 3372 3383 3401 3623 928
Size: 971 901 572 11 18 222 3741
Fragments arranged by size:
3741 971 901 572 222 18 11
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2005 2021 2039 4627 4661 1903
Size: 34 50 18 16 18 2588 34 3678
Fragments arranged by size:
3678 2588 50 34 34 18 18 16
BciVI GTATCCnnnnn_n'
Cuts at: 4328 5538 4328
Size: 1210 5226
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6436
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3206 5047 136
Size: 107 122 71 631 605 1534 1841 1525
Fragments arranged by size:
1841 1534 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2593 6431 2593
Size: 3838 2598
BlpI GC'TnA_GC
Cuts at: 3087 3387 3853 3087
Size: 300 466 5670
Fragments arranged by size:
5670 466 300
Bme1580I G_kGCm'C
Cuts at: 1790 3151 4497 5653 1790
Size: 1361 1346 1156 2573
Fragments arranged by size:
2573 1361 1346 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6436
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3665 4167 5125
Size: 416 53 83 399 24 2248 502 958
Cuts at: 5125 442
Size: 1753
Fragments arranged by size:
2248 1753 958 502 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6436
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3429 3429
Size: 6436
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2072 2618 2749 3317 5096 1239
Size: 833 546 131 568 1779 2579
Fragments arranged by size:
2579 1779 833 568 546 131
Bpu10I CC'TnA_GC
Cuts at: 2552 2552
Size: 6436
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2140 2574 3149 4561 5426 5724 2140
Size: 434 575 1412 865 298 2852
Fragments arranged by size:
2852 1412 865 575 434 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2357 2681 5099 882
Size: 617 858 324 2418 2219
Fragments arranged by size:
2418 2219 858 617 324
BsaAI yAC'GTr
Cuts at: 493 1204 4227 493
Size: 711 3023 2702
Fragments arranged by size:
3023 2702 711
BsaBI GATnn'nnATC
Cuts at: 6430 6430
Size: 6436
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4243 4625 5260 275
Size: 53 83 186 936 2710 382 635 1451
Fragments arranged by size:
2710 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4869 5267 5541 5688 6099 4869
Size: 398 274 147 411 5206
Fragments arranged by size:
5206 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6211
Fragments arranged by size:
6211 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2525 2951 3053 3533 1186
Size: 1339 426 102 480 4089
Fragments arranged by size:
4089 1339 480 426 102
BseYI C'CCAG_C
Cuts at: 2656 3291 5639 6135 2656
Size: 635 2348 496 2957
Fragments arranged by size:
2957 2348 635 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2930 3578 1163
Size: 573 1194 648 4021
Fragments arranged by size:
4021 1194 648 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3886 4647 5675 1540
Size: 275 186 1885 761 1028 2301
Fragments arranged by size:
2301 1885 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4497 4582 5653 4497
Size: 85 1071 5280
Fragments arranged by size:
5280 1071 85
BsmI GAATG_Cn'
Cuts at: 2331 3947 4040 2331
Size: 1616 93 4727
Fragments arranged by size:
4727 1616 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3121 3151 3755 4497 4582 5653 1391
Size: 399 1331 30 604 742 85 1071 2174
Fragments arranged by size:
2174 1331 1071 742 604 399 85 30
BspEI T'CCGG_A
Cuts at: 6099 6099
Size: 6436
BspHI T'CATG_A
Cuts at: 4325 6055 4325
Size: 1730 4706
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3791 844
Size: 2947 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2515 4323 1394
Size: 429 692 1808 3507
Fragments arranged by size:
3507 1808 692 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4931 5105 65
Size: 1183 3683 174 1396
Fragments arranged by size:
3683 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5080 5267 1700
Size: 110 3270 187 2869
Fragments arranged by size:
3270 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6436
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6436
BssSI C'ACGA_G
Cuts at: 4496 5508 4496
Size: 1012 5424
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3428 794
Size: 2634 3802
BstBI TT'CG_AA
Cuts at: 5240 5240
Size: 6436
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6436
BstYI r'GATC_y
Cuts at: 1972 2517 2593 2841 3687 3766 4133 4518 4535
Size: 545 76 248 846 79 367 385 17
Cuts at: 4535 5987 6073 6085 6431 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 846 545 385 367 346 248
86 79 76 17 12
Bsu36I CC'TnA_GG
Cuts at: 2262 2262
Size: 6436
BtgI C'CryG_G
Cuts at: 513 1065 2037 2542 513
Size: 552 972 505 4407
Fragments arranged by size:
4407 972 552 505
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5697
Fragments arranged by size:
5697 683 56
BtsI GCAGTG_nn'
Cuts at: 3520 3952 4746 4766 3520
Size: 432 794 20 5190
Fragments arranged by size:
5190 794 432 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6436
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6401
DraI TTT'AAA
Cuts at: 3740 3740
Size: 6436
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5443 809
Size: 507 4127 1802
Fragments arranged by size:
4127 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2445 3171 3501
Size: 54 1425 50 275 18 615 726 330
Cuts at: 3501 3591 3883 4772 6401 8
Size: 90 292 889 1629 43
Fragments arranged by size:
1629 1425 889 726 615 330 292 275
90 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3883 1537
Size: 275 2071 4090
Fragments arranged by size:
4090 2071 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3189 4366 1353
Size: 1836 1177 3423
Fragments arranged by size:
3423 1836 1177
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5023 5397 5543 387
Size: 1064 138 3434 374 146 1280
Fragments arranged by size:
3434 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2072 2600 2605 2618 2749 3077 3213
Size: 393 440 528 5 13 131 328 136
Cuts at: 3213 3317 3413 3590 4499 5096 5883 6151 1239
Size: 104 96 177 909 597 787 268 1524
Fragments arranged by size:
1524 909 787 597 528 440 393 328
268 177 136 131 104 96 13 5
EcoO109I rG'GnC_Cy
Cuts at: 3574 3574
Size: 6436
EcoRI G'AATT_C
Cuts at: 3745 4123 3745
Size: 378 6058
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2737 2860 3226 2737
Size: 123 366 5947
Fragments arranged by size:
5947 366 123
FspI TGC'GCA
Cuts at: 4942 4942
Size: 6436
HaeII r_GCGC'y
Cuts at: 1678 2305 5583 1678
Size: 627 3278 2531
Fragments arranged by size:
3278 2531 627
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2255 2287 5077 5109 5151
Size: 32 135 32 683 32 2790 32 42
Cuts at: 5151 5183 1373
Size: 32 2626
Fragments arranged by size:
2790 2626 683 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3780 4026 669
Size: 988 281 1842 246 3079
Fragments arranged by size:
3079 1842 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6436
HpaI GTT'AAC
Cuts at: 4026 4026
Size: 6436
KpnI G_GTAC'C
Cuts at: 3761 3761
Size: 6436
MfeI C'AATT_G
Cuts at: 4035 4035
Size: 6436
MluI A'CGCG_T
Cuts at: 6312 6312
Size: 6436
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5550 5734 1305
Size: 4245 184 2007
Fragments arranged by size:
4245 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3173 3503 3593 10
Size: 54 1425 343 1341 330 90 2853
Fragments arranged by size:
2853 1425 1341 343 330 90 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2906 4394 4753 4912 518
Size: 648 1740 1488 359 159 2042
Fragments arranged by size:
2042 1740 1488 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2369 3253 3295 3852 4529 5677 5922 1606
Size: 763 884 42 557 677 1148 245 2120
Fragments arranged by size:
2120 1148 884 763 677 557 245 42
NcoI C'CATG_G
Cuts at: 513 1065 2037 513
Size: 552 972 4912
Fragments arranged by size:
4912 972 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6436
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6436
NotI GC'GGCC_GC
Cuts at: 3883 3883
Size: 6436
NspI r_CATG'y
Cuts at: 1132 1229 2551 2881 3652 3794 5339 1132
Size: 97 1322 330 771 142 1545 2229
Fragments arranged by size:
2229 1545 1322 771 330 142 97
PasI CC'CwG_GG
Cuts at: 1348 2671 1348
Size: 1323 5113
PciI A'CATG_T
Cuts at: 5335 5335
Size: 6436
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6436
PfoI T'CCnGG_A
Cuts at: 2475 2568 2475
Size: 93 6343
PmeI GTTT'AAAC
Cuts at: 3740 3740
Size: 6436
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4505 4537 6018 6050 4505
Size: 32 1481 32 4891
Fragments arranged by size:
4891 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6436
PsiI TTA'TAA
Cuts at: 4006 4006
Size: 6436
PstI C_TGCA'G
Cuts at: 830 2148 3219 3788 830
Size: 1318 1071 569 3478
Fragments arranged by size:
3478 1318 1071 569
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6436
PvuII CAG'CTG
Cuts at: 3253 3295 3253
Size: 42 6394
SalI G'TCGA_C
Cuts at: 1936 3778 1936
Size: 1842 4594
SbfI CC_TGCA'GG
Cuts at: 3788 3788
Size: 6436
ScaI AGT'ACT
Cuts at: 4684 6396 4684
Size: 1712 4724
SexAI A'CCwGG_T
Cuts at: 2097 2097
Size: 6436
SfcI C'TryA_G
Cuts at: 826 1045 2144 3215 3784 4919 5600 5791 826
Size: 219 1099 1071 569 1135 681 191 1471
Fragments arranged by size:
1471 1135 1099 1071 681 569 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3206 3206
Size: 6436
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6436
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6436
SmaI CCC'GGG
Cuts at: 1872 2933 3227 3763 1872
Size: 1061 294 536 4545
Fragments arranged by size:
4545 1061 536 294
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2119 2589 3128 4540 5441 5703
Size: 197 926 176 470 539 1412 901 262
Cuts at: 5703 820
Size: 1553
Fragments arranged by size:
1553 1412 926 901 539 470 262 197
176
SnaBI TAC'GTA
Cuts at: 493 4227 493
Size: 3734 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6436
SphI G_CATG'C
Cuts at: 1132 2551 3794 1132
Size: 1419 1243 3774
Fragments arranged by size:
3774 1419 1243
SspI AAT'ATT
Cuts at: 5 52 4360 5
Size: 47 4308 2081
Fragments arranged by size:
4308 2081 47
StuI AGG'CCT
Cuts at: 1517 3209 3221 1517
Size: 1692 12 4732
Fragments arranged by size:
4732 1692 12
StyI C'CwwG_G
Cuts at: 513 1065 2037 3174 3201 3258 3504 3875 513
Size: 552 972 1137 27 57 246 371 3074
Fragments arranged by size:
3074 1137 972 552 371 246 57 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4463 4480 4633 4818 5263 325
Size: 1255 2883 17 153 185 445 1498
Fragments arranged by size:
2883 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3407 4682 6394
Size: 274 80 33 51 1443 1430 1275 1712
Cuts at: 6394 96
Size: 138
Fragments arranged by size:
1712 1443 1430 1275 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6396 6428 6396
Size: 32 6404
Tth111I GACn'n_nGTC
Cuts at: 1316 2804 1316
Size: 1488 4948
XbaI T'CTAG_A
Cuts at: 3772 3772
Size: 6436
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2402 2402
Size: 6436
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6436
XmaI C'CCGG_G
Cuts at: 1870 2931 3225 3761 1870
Size: 1061 294 536 4545
Fragments arranged by size:
4545 1061 536 294
XmnI GAAnn'nnTTC
Cuts at: 2283 2618 4565 2283
Size: 335 1947 4154
Fragments arranged by size:
4154 1947 335
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4244 276
Size: 53 83 186 936 2710 2468
Fragments arranged by size:
2710 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SfoI SrfI SwaI