Restriction map for
pFN21AB3463
(Circular) (Six-base) MAPSORT of: pFN21AB3463.seq Check: 6488 from: 1 to: 9508
pFN21AB3463 9508 bp
With 182 enzymes: SgfI *
October 20, 2009 10:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7318 278
Size: 53 83 186 936 5782 2468
Fragments arranged by size:
5782 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6851 1937
Size: 4914 4594
Acc65I G'GTAC_C
Cuts at: 5420 6829 5420
Size: 1409 8099
AclI AA'CG_TT
Cuts at: 1563 7635 8008 1563
Size: 6072 373 3063
Fragments arranged by size:
6072 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2325 4224 4785 5534 5670 5928 6228 6414
Size: 693 1899 561 749 136 258 300 186
Cuts at: 6414 7571 8955 9223 1632
Size: 1157 1384 268 1917
Fragments arranged by size:
1917 1899 1384 1157 749 693 561 300
268 258 186 136
AflII C'TTAA_G
Cuts at: 820 1017 3412 820
Size: 197 2395 6916
Fragments arranged by size:
6916 2395 197
AflIII A'CryG_T
Cuts at: 1203 2666 6327 8407 9384 1203
Size: 1463 3661 2080 977 1327
Fragments arranged by size:
3661 2080 1463 1327 977
AgeI A'CCGG_T
Cuts at: 4364 8339 4364
Size: 3975 5533
AhdI GACnn_n'nnGTC
Cuts at: 5785 5854 8237 5785
Size: 69 2383 7056
Fragments arranged by size:
7056 2383 69
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 9508
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5303 1184
Size: 4119 5389
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4832 4864 6091 6123 4832
Size: 32 1227 32 8217
Fragments arranged by size:
8217 1227 32 32
AlwNI CAG_nnn'CTG
Cuts at: 5896 5906 7251 8823 5896
Size: 10 1345 1572 6581
Fragments arranged by size:
6581 1572 1345 10
ApaLI G'TGCA_C
Cuts at: 7565 8721 7565
Size: 1156 8352
ApoI r'AATT_y
Cuts at: 1493 2400 3013 3138 3506 3792 4352 4467 4678
Size: 907 613 125 368 286 560 115 211
Cuts at: 4678 4755 5227 5434 6817 7043 7195 9304 1493
Size: 77 472 207 1383 226 152 2109 1697
Fragments arranged by size:
2109 1697 1383 907 613 560 472 368
286 226 211 207 152 125 115 77
AseI AT'TA_AT
Cuts at: 160 1032 2147 8062 160
Size: 872 1115 5915 1606
Fragments arranged by size:
5915 1606 1115 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9508
AvaI C'yCGr_G
Cuts at: 1870 1943 2057 2069 6833 1870
Size: 73 114 12 4764 4545
Fragments arranged by size:
4764 4545 114 73 12
BamHI G'GATC_C
Cuts at: 3310 6838 7205 3310
Size: 3528 367 5613
Fragments arranged by size:
5613 3528 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5420 5554 6829 8284 618
Size: 325 217 627 3633 134 1275 1455 1842
Fragments arranged by size:
3633 1842 1455 1275 627 325 217 134
BanII G_rGCy'C
Cuts at: 1391 6234 6827 1391
Size: 4843 593 4072
Fragments arranged by size:
4843 4072 593
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2556 2663 3836 4438 5685 928
Size: 971 657 107 1173 602 1247 4751
Fragments arranged by size:
4751 1247 1173 971 657 602 107
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7699 7733 1903
Size: 34 5762 34 3678
Fragments arranged by size:
5762 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7400 8610 7400
Size: 1210 8298
BclI T'GATC_A
Cuts at: 1548 3252 3730 4252 1548
Size: 1704 478 522 6804
Fragments arranged by size:
6804 1704 522 478
BfrBI ATG'CAT
Cuts at: 2524 3070 3283 6631 2524
Size: 546 213 3348 5401
Fragments arranged by size:
5401 3348 546 213
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5324 6718 8119
Size: 107 122 71 631 605 3652 1394 1401
Cuts at: 8119 136
Size: 1525
Fragments arranged by size:
3652 1525 1401 1394 631 605 122 107
71
BglII A'GATC_T
Cuts at: 2752 4948 9503 2752
Size: 2196 4555 2757
Fragments arranged by size:
4555 2757 2196
BlpI GC'TnA_GC
Cuts at: 6925 6925
Size: 9508
Bme1580I G_kGCm'C
Cuts at: 1790 5275 7569 8725 1790
Size: 3485 2294 1156 2573
Fragments arranged by size:
3485 2573 2294 1156
BmgBI CAC'GTC
Cuts at: 1318 2669 1318
Size: 1351 8157
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5925 6419 7239
Size: 416 53 83 399 24 4508 494 820
Cuts at: 7239 8197 442
Size: 958 1753
Fragments arranged by size:
4508 1753 958 820 494 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9508
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2968 2968
Size: 9508
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2840 2937 5460 5779 6295 6513 6576 8168
Size: 1601 97 2523 319 516 218 63 1592
Cuts at: 8168 1239
Size: 2579
Fragments arranged by size:
2579 2523 1601 1592 516 319 218 97
63
Bpu10I CC'TnA_GC
Cuts at: 3267 4097 3267
Size: 830 8678
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4524 5715 6036 7633 8498 8796 4524
Size: 1191 321 1597 865 298 5236
Fragments arranged by size:
5236 1597 1191 865 321 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 8171 882
Size: 617 6672 2219
Fragments arranged by size:
6672 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 6330 7299 493
Size: 711 5126 969 2702
Fragments arranged by size:
5126 2702 969 711
BsaBI GATnn'nnATC
Cuts at: 2625 9502 2625
Size: 6877 2631
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5696 7315 7697 8332
Size: 53 83 186 936 4163 1619 382 635
Cuts at: 8332 275
Size: 1451
Fragments arranged by size:
4163 1619 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3222 3549 4364 7941 8339 8613 8760 9171 3222
Size: 327 815 3577 398 274 147 411 3559
Fragments arranged by size:
3577 3559 815 411 398 327 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3177 3207 1178
Size: 30 165 30 1774 30 7479
Fragments arranged by size:
7479 1774 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 5390 5718 6298 6516 1186
Size: 4204 328 580 218 4178
Fragments arranged by size:
4204 4178 580 328 218
BseYI C'CCAG_C
Cuts at: 3120 4602 5182 6156 6379 8711 9207 3120
Size: 1482 580 974 223 2332 496 3421
Fragments arranged by size:
3421 2332 1482 974 580 496 223
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 5592 6632 1163
Size: 573 3856 1040 4039
Fragments arranged by size:
4039 3856 1040 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6958 7719 8747 1540
Size: 275 186 4957 761 1028 2301
Fragments arranged by size:
4957 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2164 5260 5940 6377 7569 7654 8725 2164
Size: 3096 680 437 1192 85 1071 2947
Fragments arranged by size:
3096 2947 1192 1071 680 437 85
BsmI GAATG_Cn'
Cuts at: 2368 7019 7112 2368
Size: 4651 93 4764
Fragments arranged by size:
4764 4651 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3278 3278
Size: 9508
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2164 5260 5275 5940 6234 6377 6827
Size: 399 374 3096 15 665 294 143 450
Cuts at: 6827 7569 7654 8725 1391
Size: 742 85 1071 2174
Fragments arranged by size:
3096 2174 1071 742 665 450 399 374
294 143 85 15
BspEI T'CCGG_A
Cuts at: 9171 9171
Size: 9508
BspHI T'CATG_A
Cuts at: 4348 7397 9127 4348
Size: 3049 1730 4729
Fragments arranged by size:
4729 3049 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2791 6863 844
Size: 1947 4072 3489
Fragments arranged by size:
4072 3489 1947
BsrBI CCG'CTC
Cuts at: 1394 1823 2062 7395 1394
Size: 429 239 5333 3507
Fragments arranged by size:
5333 3507 429 239
BsrDI GCAATG_nn'
Cuts at: 65 1248 2586 2738 6043 6201 8003 8177 65
Size: 1183 1338 152 3305 158 1802 174 1396
Fragments arranged by size:
3305 1802 1396 1338 1183 174 158 152
BsrFI r'CCGG_y
Cuts at: 1700 1810 4364 8152 8339 1700
Size: 110 2554 3788 187 2869
Fragments arranged by size:
3788 2869 2554 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9508
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9508
BssSI C'ACGA_G
Cuts at: 6677 7568 8580 6677
Size: 891 1012 7605
Fragments arranged by size:
7605 1012 891
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3067 6727 794
Size: 2273 3660 3575
Fragments arranged by size:
3660 3575 2273
BstBI TT'CG_AA
Cuts at: 5158 8312 5158
Size: 3154 6354
BstEII G'GTnAC_C
Cuts at: 1184 3476 1184
Size: 2292 7216
BstXI CCAn_nnnn'nTGG
Cuts at: 4222 4594 6156 4222
Size: 372 1562 7574
Fragments arranged by size:
7574 1562 372
BstYI r'GATC_y
Cuts at: 1972 2243 2734 2752 3310 4948 6838 7205 7590
Size: 271 491 18 558 1638 1890 367 385
Cuts at: 7590 7607 9059 9145 9157 9503 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1890 1638 1452 558 491 385 367
346 271 86 18 17 12
Bsu36I CC'TnA_GG
Cuts at: 4410 4410
Size: 9508
BtgI C'CryG_G
Cuts at: 513 1065 2016 2265 513
Size: 552 951 249 7756
Fragments arranged by size:
7756 951 552 249
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8769
Fragments arranged by size:
8769 683 56
BtsI GCAGTG_nn'
Cuts at: 6278 6615 7024 7818 7838 6278
Size: 337 409 794 20 7948
Fragments arranged by size:
7948 794 409 337 20
ClaI AT'CG_AT
Cuts at: 1554 4659 1554
Size: 3105 6403
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9473
DraI TTT'AAA
Cuts at: 2970 3144 6812 2970
Size: 174 3668 5666
Fragments arranged by size:
5666 3668 174
DraIII CAC_nnn'GTG
Cuts at: 3250 3250
Size: 9508
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2560 8515 809
Size: 507 1244 5955 1802
Fragments arranged by size:
5955 1802 1244 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4596 5497 6955
Size: 54 1425 50 275 18 2766 901 1458
Cuts at: 6955 7844 9473 8
Size: 889 1629 43
Fragments arranged by size:
2766 1629 1458 1425 901 889 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6955 1537
Size: 275 5143 4090
Fragments arranged by size:
5143 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2505 2700 2712 3174 4700 5045 6036 7438
Size: 1152 195 12 462 1526 345 991 1402
Cuts at: 7438 1353
Size: 3423
Fragments arranged by size:
3423 1526 1402 1152 991 462 345 195
12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5135 8095 8469 8615 387
Size: 1064 138 3546 2960 374 146 1280
Fragments arranged by size:
3546 2960 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2325 2840 2937 4224 4785 5460 5534
Size: 393 693 515 97 1287 561 675 74
Cuts at: 5534 5670 5779 5928 6228 6295 6414 6513 6576
Size: 136 109 149 300 67 119 99 63
Cuts at: 6576 7571 8168 8955 9223 1239
Size: 995 597 787 268 1524
Fragments arranged by size:
1524 1287 995 787 693 675 597 561
515 393 300 268 149 136 119 109
99 97 74 67 63
EcoNI CCTnn'n_nnAGG
Cuts at: 5745 6747 5745
Size: 1002 8506
EcoO109I rG'GnC_Cy
Cuts at: 2681 5893 6569 2681
Size: 3212 676 5620
Fragments arranged by size:
5620 3212 676
EcoRI G'AATT_C
Cuts at: 4467 6817 7195 4467
Size: 2350 378 6780
Fragments arranged by size:
6780 2350 378
EcoRV GAT'ATC
Cuts at: 2623 4246 2623
Size: 1623 7885
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3875 6117 3875
Size: 2242 7266
FspI TGC'GCA
Cuts at: 8014 8014
Size: 9508
HaeII r_GCGC'y
Cuts at: 1678 2031 2458 8655 1678
Size: 353 427 6197 2531
Fragments arranged by size:
6197 2531 427 353
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3526 3558 4047 4079 4433
Size: 32 135 32 1954 32 489 32 354
Cuts at: 4433 4465 5435 5467 5771 5803 5840 5872 5941
Size: 32 970 32 304 32 37 32 69
Cuts at: 5941 5973 6691 6723 8149 8181 8223 8255 1373
Size: 32 718 32 1426 32 42 32 2626
Fragments arranged by size:
2626 1954 1426 970 718 489 354 304
135 69 42 37 32 32 32 32
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2485 5848 5966 6852 7098 669
Size: 988 281 547 3363 118 886 246 3079
Fragments arranged by size:
3363 3079 988 886 547 281 246 118
HindIII A'AGCT_T
Cuts at: 748 748
Size: 9508
HpaI GTT'AAC
Cuts at: 2485 7098 2485
Size: 4613 4895
KpnI G_GTAC'C
Cuts at: 5424 6833 5424
Size: 1409 8099
MfeI C'AATT_G
Cuts at: 2813 5836 7107 2813
Size: 3023 1271 5214
Fragments arranged by size:
5214 3023 1271
MluI A'CGCG_T
Cuts at: 9384 9384
Size: 9508
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3338 4203 4398 6077 6281 8622 8806 1305
Size: 2033 865 195 1679 204 2341 184 2007
Fragments arranged by size:
2341 2033 2007 1679 865 204 195 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4598 5499 10
Size: 54 1425 343 2766 901 4019
Fragments arranged by size:
4019 2766 1425 901 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2858 2997 3462 3842 4217 4592 5401
Size: 648 1692 139 465 380 375 375 809
Cuts at: 5401 6192 7466 7825 7984 518
Size: 791 1274 359 159 2042
Fragments arranged by size:
2042 1692 1274 809 791 648 465 380
375 375 359 159 139
MspA1I CmG'CkG
Cuts at: 1606 2507 4606 5495 5561 6077 6383 6924 7601
Size: 901 2099 889 66 516 306 541 677
Cuts at: 7601 8749 8994 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 2099 1148 901 889 677 541 516
306 245 66
NcoI C'CATG_G
Cuts at: 513 1065 2265 513
Size: 552 1200 7756
Fragments arranged by size:
7756 1200 552
NdeI CA'TA_TG
Cuts at: 387 5416 387
Size: 5029 4479
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9508
NotI GC'GGCC_GC
Cuts at: 6955 6955
Size: 9508
NsiI A_TGCA'T
Cuts at: 2526 3072 3285 6633 2526
Size: 546 213 3348 5401
Fragments arranged by size:
5401 3348 546 213
NspI r_CATG'y
Cuts at: 1132 1229 3070 3947 5239 6866 8411 1132
Size: 97 1841 877 1292 1627 1545 2229
Fragments arranged by size:
2229 1841 1627 1545 1292 877 97
PasI CC'CwG_GG
Cuts at: 1348 5975 1348
Size: 4627 4881
PciI A'CATG_T
Cuts at: 8407 8407
Size: 9508
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2223 6344 1108
Size: 1115 4121 4272
Fragments arranged by size:
4272 4121 1115
PfoI T'CCnGG_A
Cuts at: 6529 6529
Size: 9508
PmeI GTTT'AAAC
Cuts at: 6812 6812
Size: 9508
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2742 2774 5366 5398 5824 5856 7577 7609 9090
Size: 32 2592 32 426 32 1721 32 1481
Cuts at: 9090 9122 2742
Size: 32 3128
Fragments arranged by size:
3128 2592 1721 1481 426 32 32 32
32 32
PpuMI rG'GwC_Cy
Cuts at: 2681 6569 2681
Size: 3888 5620
PshAI GACnn'nnGTC
Cuts at: 1613 5203 6084 1613
Size: 3590 881 5037
Fragments arranged by size:
5037 3590 881
PsiI TTA'TAA
Cuts at: 4262 6432 7078 4262
Size: 2170 646 6692
Fragments arranged by size:
6692 2170 646
PstI C_TGCA'G
Cuts at: 830 2788 4206 4578 5863 6860 830
Size: 1958 1418 372 1285 997 3478
Fragments arranged by size:
3478 1958 1418 1285 997 372
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9508
PvuII CAG'CTG
Cuts at: 2507 4606 5495 5561 6077 6383 2507
Size: 2099 889 66 516 306 5632
Fragments arranged by size:
5632 2099 889 516 306 66
SalI G'TCGA_C
Cuts at: 1936 6850 1936
Size: 4914 4594
SbfI CC_TGCA'GG
Cuts at: 6860 6860
Size: 9508
ScaI AGT'ACT
Cuts at: 7756 9468 7756
Size: 1712 7796
SexAI A'CCwGG_T
Cuts at: 3691 3691
Size: 9508
SfcI C'TryA_G
Cuts at: 826 1045 2784 4138 4202 4574 4887 5859 5867
Size: 219 1739 1354 64 372 313 972 8
Cuts at: 5867 6856 7991 8672 8863 826
Size: 989 1135 681 191 1471
Fragments arranged by size:
1739 1471 1354 1135 989 972 681 372
313 219 191 64 8
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9508
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9508
SmaI CCC'GGG
Cuts at: 1872 6835 1872
Size: 4963 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3412 4539 5730 6051 7612 8513
Size: 197 926 1469 1127 1191 321 1561 901
Cuts at: 8513 8775 820
Size: 262 1553
Fragments arranged by size:
1561 1553 1469 1191 1127 926 901 321
262 197
SnaBI TAC'GTA
Cuts at: 493 7299 493
Size: 6806 2702
SpeI A'CTAG_T
Cuts at: 152 4546 152
Size: 4394 5114
SphI G_CATG'C
Cuts at: 1132 6866 1132
Size: 5734 3774
SspI AAT'ATT
Cuts at: 5 52 2115 3562 3769 4639 4999 7432 5
Size: 47 2063 1447 207 870 360 2433 2081
Fragments arranged by size:
2433 2081 2063 1447 870 360 207 47
StuI AGG'CCT
Cuts at: 1517 4095 1517
Size: 2578 6930
StyI C'CwwG_G
Cuts at: 513 1065 2265 5136 6408 6947 513
Size: 552 1200 2871 1272 539 3074
Fragments arranged by size:
3074 2871 1272 1200 552 539
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4616 5967 6502 7535 7552 7705 7890
Size: 1255 3036 1351 535 1033 17 153 185
Cuts at: 7890 8335 325
Size: 445 1498
Fragments arranged by size:
3036 1498 1351 1255 1033 535 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3063 4653 6449
Size: 274 80 33 51 1443 1086 1590 1796
Cuts at: 6449 6471 7754 9466 96
Size: 22 1283 1712 138
Fragments arranged by size:
1796 1712 1590 1443 1283 1086 274 138
80 51 33 22
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3234 3266 5746 5778 5864 5896 9468 9500 3234
Size: 32 2480 32 86 32 3572 32 3242
Fragments arranged by size:
3572 3242 2480 86 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2860 1316
Size: 1544 7964
XbaI T'CTAG_A
Cuts at: 3499 4973 6257 6644 6844 3499
Size: 1474 1284 387 200 6163
Fragments arranged by size:
6163 1474 1284 387 200
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4219 5920 4219
Size: 1701 7807
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9508
XmaI C'CCGG_G
Cuts at: 1870 6833 1870
Size: 4963 4545
XmnI GAAnn'nnTTC
Cuts at: 3330 5131 5164 7637 3330
Size: 1801 33 2473 5201
Fragments arranged by size:
5201 2473 1801 33
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7316 276
Size: 53 83 186 936 5782 2468
Fragments arranged by size:
5782 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BsiWI BstZ17I EcoICRI FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SfiI SfoI SrfI SwaI