Restriction map for
pFN21AB3458
(Circular) (Six-base) MAPSORT of: pFN21AB3458.seq Check: 6551 from: 1 to: 8056
pFN21AB3458 8056 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2602 3048 3852 2602
Size: 446 804 6806
Fragments arranged by size:
6806 804 446
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5253 5866 278
Size: 53 83 186 936 3717 613 2468
Fragments arranged by size:
3717 2468 936 613 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3077 5399 1937
Size: 1140 2322 4594
Fragments arranged by size:
4594 2322 1140
Acc65I G'GTAC_C
Cuts at: 5377 5377
Size: 8056
AclI AA'CG_TT
Cuts at: 1563 6183 6556 1563
Size: 4620 373 3063
Fragments arranged by size:
4620 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2651 3245 3578 3910 5307 5321 6119 7503
Size: 1019 594 333 332 1397 14 798 1384
Cuts at: 7503 7771 1632
Size: 268 1917
Fragments arranged by size:
1917 1397 1384 1019 798 594 333 332
268 14
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7859
AflIII A'CryG_T
Cuts at: 1203 4542 6955 7932 1203
Size: 3339 2413 977 1327
Fragments arranged by size:
3339 2413 1327 977
AgeI A'CCGG_T
Cuts at: 6887 6887
Size: 8056
AhdI GACnn_n'nnGTC
Cuts at: 6785 6785
Size: 8056
AleI CACnn'nnGTG
Cuts at: 1166 2447 1166
Size: 1281 6775
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3540 1184
Size: 2356 5700
AlwNI CAG_nnn'CTG
Cuts at: 2020 2276 2500 2557 2722 2755 2773 3415 4064
Size: 256 224 57 165 33 18 642 649
Cuts at: 4064 4771 4843 5799 7371 2020
Size: 707 72 956 1572 2705
Fragments arranged by size:
2705 1572 956 707 649 642 256 224
165 72 57 33 18
ApaI G_GGCC'C
Cuts at: 5169 5169
Size: 8056
ApaLI G'TGCA_C
Cuts at: 6113 7269 6113
Size: 1156 6900
ApoI r'AATT_y
Cuts at: 1493 2472 2886 4246 4292 5365 5591 5743 7852
Size: 979 414 1360 46 1073 226 152 2109
Cuts at: 7852 1493
Size: 1697
Fragments arranged by size:
2109 1697 1360 1073 979 414 226 152
46
AseI AT'TA_AT
Cuts at: 160 1032 6610 160
Size: 872 5578 1606
Fragments arranged by size:
5578 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8056
AvaI C'yCGr_G
Cuts at: 1870 1943 2242 3330 3787 3833 3916 4893 5381
Size: 73 299 1088 457 46 83 977 488
Cuts at: 5381 1870
Size: 4545
Fragments arranged by size:
4545 1088 977 488 457 299 83 73
46
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2963 2996 2963
Size: 33 8023
BamHI G'GATC_C
Cuts at: 3378 5386 5753 3378
Size: 2008 367 5681
Fragments arranged by size:
5681 2008 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2332 2614 2694 2731 3720
Size: 325 217 627 545 282 80 37 989
Cuts at: 3720 3791 3959 4702 4871 4898 4931 5176 5377
Size: 71 168 743 169 27 33 245 201
Cuts at: 5377 6832 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 989 743 627 545 325 282
245 217 201 169 168 80 71 37
33 27
BanII G_rGCy'C
Cuts at: 1391 2352 2533 2580 3122 3150 3269 3320 3466
Size: 961 181 47 542 28 119 51 146
Cuts at: 3466 3687 3902 3917 3924 4964 5169 5375 1391
Size: 221 215 15 7 1040 205 206 4072
Fragments arranged by size:
4072 1040 961 542 221 215 206 205
181 146 119 51 47 28 15 7
BbeI G_GCGC'C
Cuts at: 3795 3963 5180 3795
Size: 168 1217 6671
Fragments arranged by size:
6671 1217 168
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3233 5029 928
Size: 971 1334 1796 3955
Fragments arranged by size:
3955 1796 1334 971
BbvCI CC'TCA_GC
Cuts at: 3402 3402
Size: 8056
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2870 2904 6247 6281 1903
Size: 34 933 34 3343 34 3678
Fragments arranged by size:
3678 3343 933 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2230 5948 7158 2230
Size: 3718 1210 3128
Fragments arranged by size:
3718 3128 1210
BclI T'GATC_A
Cuts at: 1548 5211 5317 1548
Size: 3663 106 4287
Fragments arranged by size:
4287 3663 106
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2150 6667 136
Size: 107 122 71 631 605 478 4517 1525
Fragments arranged by size:
4517 1525 631 605 478 122 107 71
BglII A'GATC_T
Cuts at: 3322 8051 3322
Size: 4729 3327
BlpI GC'TnA_GC
Cuts at: 2346 3263 3896 5473 2346
Size: 917 633 1577 4929
Fragments arranged by size:
4929 1577 917 633
Bme1580I G_kGCm'C
Cuts at: 1790 2335 2619 2697 3672 4874 5169 6117 7273
Size: 545 284 78 975 1202 295 948 1156
Cuts at: 7273 1790
Size: 2573
Fragments arranged by size:
2573 1202 1156 975 948 545 295 284
78
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8056
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3124 3366 3417
Size: 416 53 83 399 24 1707 242 51
Cuts at: 3417 5787 6745 442
Size: 2370 958 1753
Fragments arranged by size:
2370 1753 1707 958 416 399 242 83
53 51 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8056
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4086 4205 6716 1239
Size: 2847 119 2511 2579
Fragments arranged by size:
2847 2579 2511 119
Bpu10I CC'TnA_GC
Cuts at: 2727 3402 2727
Size: 675 7381
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2163 3674 4129 6181 7046 7344 2163
Size: 1511 455 2052 865 298 2875
Fragments arranged by size:
2875 2052 1511 865 455 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3300 6719 882
Size: 617 1801 3419 2219
Fragments arranged by size:
3419 2219 1801 617
BsaAI yAC'GTr
Cuts at: 493 1204 2297 4545 5847 493
Size: 711 1093 2248 1302 2702
Fragments arranged by size:
2702 2248 1302 1093 711
BsaBI GATnn'nnATC
Cuts at: 8050 8050
Size: 8056
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2085 3792 3960 3983
Size: 53 83 186 936 552 1707 168 23
Cuts at: 3983 3992 5177 5250 5863 6245 6880 275
Size: 9 1185 73 613 382 635 1451
Fragments arranged by size:
1707 1451 1185 936 635 613 552 382
186 168 83 73 53 23 9
BsaWI w'CCGG_w
Cuts at: 2394 4578 6489 6887 7161 7308 7719 2394
Size: 2184 1911 398 274 147 411 2731
Fragments arranged by size:
2731 2184 1911 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2360 2390 1178
Size: 30 165 30 957 30 6844
Fragments arranged by size:
6844 957 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3695 4655 4658 4661 4664 4667 1186
Size: 2509 960 3 3 3 3 4575
Fragments arranged by size:
4575 2509 960 3 3 3 3
BseYI C'CCAG_C
Cuts at: 2581 2619 3150 3195 3310 3465 4564 7259 7755
Size: 38 531 45 115 155 1099 2695 496
Cuts at: 7755 2581
Size: 2882
Fragments arranged by size:
2882 2695 1099 531 496 155 115 45
38
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2486 2759 3719 1163
Size: 573 750 273 960 5500
Fragments arranged by size:
5500 960 750 573 273
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3728 5506 6267 7295 1540
Size: 275 186 1727 1778 761 1028 2301
Fragments arranged by size:
2301 1778 1727 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2545 3269 3320 4964 6117 6202 7273 2545
Size: 724 51 1644 1153 85 1071 3328
Fragments arranged by size:
3328 1644 1153 1071 724 85 51
BsmI GAATG_Cn'
Cuts at: 2122 5567 5660 2122
Size: 3445 93 4518
Fragments arranged by size:
4518 3445 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2335 2352 2533 2545 2580 2619 2697
Size: 399 545 17 181 12 35 39 78
Cuts at: 2697 3122 3150 3269 3320 3466 3672 3687 3902
Size: 425 28 119 51 146 206 15 215
Cuts at: 3902 3917 3924 4874 4964 5169 5375 6117 6202
Size: 15 7 950 90 205 206 742 85
Cuts at: 6202 7273 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 950 742 545 425 399 215
206 206 205 181 146 119 90 85
78 51 39 35 28 17 15 15
12 7
BspEI T'CCGG_A
Cuts at: 2394 7719 2394
Size: 5325 2731
BspHI T'CATG_A
Cuts at: 5945 7675 5945
Size: 1730 6326
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2475 2602 3048 3582 3852 5411 844
Size: 1631 127 446 534 270 1559 3489
Fragments arranged by size:
3489 1631 1559 534 446 270 127
BsrBI CCG'CTC
Cuts at: 1394 1823 5943 1394
Size: 429 4120 3507
Fragments arranged by size:
4120 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3937 4265 6551 6725 65
Size: 1183 2689 328 2286 174 1396
Fragments arranged by size:
2689 2286 1396 1183 328 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2587 3723 4409 6700 6887 1700
Size: 110 777 1136 686 2291 187 2869
Fragments arranged by size:
2869 2291 1136 777 686 187 110
BsrGI T'GTAC_A
Cuts at: 96 2022 4489 96
Size: 1926 2467 3663
Fragments arranged by size:
3663 2467 1926
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8056
BssSI C'ACGA_G
Cuts at: 3863 6116 7128 3863
Size: 2253 1012 4791
Fragments arranged by size:
4791 2253 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2794 3010 3034 794
Size: 2000 216 24 5816
Fragments arranged by size:
5816 2000 216 24
BstBI TT'CG_AA
Cuts at: 6860 6860
Size: 8056
BstEII G'GTnAC_C
Cuts at: 1184 2009 4051 4882 1184
Size: 825 2042 831 4358
Fragments arranged by size:
4358 2042 831 825
BstXI CCAn_nnnn'nTGG
Cuts at: 2038 2111 2297 3956 4516 2038
Size: 73 186 1659 560 5578
Fragments arranged by size:
5578 1659 560 186 73
BstYI r'GATC_y
Cuts at: 1972 2391 3322 3378 5386 5753 6138 6155 7607
Size: 419 931 56 2008 367 385 17 1452
Cuts at: 7607 7693 7705 8051 1972
Size: 86 12 346 1977
Fragments arranged by size:
2008 1977 1452 931 419 385 367 346
86 56 17 12
BstZ17I GTA'TAC
Cuts at: 3078 3078
Size: 8056
Bsu36I CC'TnA_GG
Cuts at: 2148 4783 2148
Size: 2635 5421
BtgI C'CryG_G
Cuts at: 513 1065 2004 2044 2571 3271 3604 3949 4001
Size: 552 939 40 527 700 333 345 52
Cuts at: 4001 4746 4864 5169 513
Size: 745 118 305 3400
Fragments arranged by size:
3400 939 745 700 552 527 345 333
305 118 52 40
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3338 487
Size: 683 56 2112 5205
Fragments arranged by size:
5205 2112 683 56
BtsI GCAGTG_nn'
Cuts at: 2523 2753 5572 6366 6386 2523
Size: 230 2819 794 20 4193
Fragments arranged by size:
4193 2819 794 230 20
ClaI AT'CG_AT
Cuts at: 1554 4032 1554
Size: 2478 5578
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5008 5043 529
Size: 35 4444 35 3542
Fragments arranged by size:
4444 3542 35 35
DraI TTT'AAA
Cuts at: 5360 5360
Size: 8056
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5249 5252 7063 809
Size: 507 3933 3 1811 1802
Fragments arranged by size:
3933 1811 1802 507 3
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2227 2307 3490
Size: 54 1425 50 275 18 397 80 1183
Cuts at: 3490 3589 3725 3946 4196 4849 5503 6392 8021
Size: 99 136 221 250 653 654 889 1629
Cuts at: 8021 8
Size: 43
Fragments arranged by size:
1629 1425 1183 889 654 653 397 275
250 221 136 99 80 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3725 5503 1537
Size: 275 1913 1778 4090
Fragments arranged by size:
4090 1913 1778 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2670 3743 3929 4626 4686 5274 5986 1353
Size: 1317 1073 186 697 60 588 712 3423
Fragments arranged by size:
3423 1317 1073 712 697 588 186 60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2148 2537 4585 6643 7017 7163
Size: 1064 138 559 389 2048 2058 374 146
Cuts at: 7163 387
Size: 1280
Fragments arranged by size:
2058 2048 1280 1064 559 389 374 146
138
EcoICRI GAG'CTC
Cuts at: 3267 3318 4962 3267
Size: 51 1644 6361
Fragments arranged by size:
6361 1644 51
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2651 3245 3578 3910 4086 4205 5307
Size: 393 1019 594 333 332 176 119 1102
Cuts at: 5307 5321 6119 6716 7503 7771 1239
Size: 14 798 597 787 268 1524
Fragments arranged by size:
1524 1102 1019 798 787 597 594 393
333 332 268 176 119 14
EcoNI CCTnn'n_nnAGG
Cuts at: 2688 3294 3635 4157 2688
Size: 606 341 522 6587
Fragments arranged by size:
6587 606 522 341
EcoO109I rG'GnC_Cy
Cuts at: 2273 2761 3064 3277 3284 3398 3474 3618 4061
Size: 488 303 213 7 114 76 144 443
Cuts at: 4061 4657 4768 5165 2273
Size: 596 111 397 5164
Fragments arranged by size:
5164 596 488 443 397 303 213 144
114 111 76 7
EcoRI G'AATT_C
Cuts at: 2472 5365 5743 2472
Size: 2893 378 4785
Fragments arranged by size:
4785 2893 378
EcoRV GAT'ATC
Cuts at: 4144 4144
Size: 8056
FspI TGC'GCA
Cuts at: 6562 6562
Size: 8056
HaeII r_GCGC'y
Cuts at: 1678 3795 3963 5180 7203 1678
Size: 2117 168 1217 2023 2531
Fragments arranged by size:
2531 2117 2023 1217 168
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3307 3339 4324 4356 4504
Size: 32 135 32 1735 32 985 32 148
Cuts at: 4504 4536 4661 4693 4887 4919 6697 6729 6771
Size: 32 125 32 194 32 1778 32 42
Cuts at: 6771 6803 1373
Size: 32 2626
Fragments arranged by size:
2626 1778 1735 985 194 148 135 125
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2482 5400 5646 669
Size: 988 281 544 2918 246 3079
Fragments arranged by size:
3079 2918 988 544 281 246
HindIII A'AGCT_T
Cuts at: 748 2405 748
Size: 1657 6399
HpaI GTT'AAC
Cuts at: 5646 5646
Size: 8056
KasI G'GCGC_C
Cuts at: 3791 3959 5176 3791
Size: 168 1217 6671
Fragments arranged by size:
6671 1217 168
KpnI G_GTAC'C
Cuts at: 5381 5381
Size: 8056
MfeI C'AATT_G
Cuts at: 5655 5655
Size: 8056
MluI A'CGCG_T
Cuts at: 7932 7932
Size: 8056
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4047 7170 7354 1305
Size: 2742 3123 184 2007
Fragments arranged by size:
3123 2742 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2229 2309 3492 3591 4198
Size: 54 1425 343 397 80 1183 99 607
Cuts at: 4198 4851 10
Size: 653 3215
Fragments arranged by size:
3215 1425 1183 653 607 397 343 99
80 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2049 2109 2447 3299 4183 6014 6373
Size: 648 883 60 338 852 884 1831 359
Cuts at: 6373 6532 518
Size: 159 2042
Fragments arranged by size:
2042 1831 884 883 852 648 359 338
159 60
MspA1I CmG'CkG
Cuts at: 1606 2094 2415 2554 2944 2953 3106 3223 3415
Size: 488 321 139 390 9 153 117 192
Cuts at: 3415 3814 3973 4986 5132 5203 5472 6149 7297
Size: 399 159 1013 146 71 269 677 1148
Cuts at: 7297 7542 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 1013 677 488 399 390 321
269 245 192 159 153 146 139 117
71 9
NarI GG'CG_CC
Cuts at: 3792 3960 5177 3792
Size: 168 1217 6671
Fragments arranged by size:
6671 1217 168
NcoI C'CATG_G
Cuts at: 513 1065 2004 2044 2571 3271 513
Size: 552 939 40 527 700 5298
Fragments arranged by size:
5298 939 700 552 527 40
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8056
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8056
NotI GC'GGCC_GC
Cuts at: 5503 5503
Size: 8056
NspI r_CATG'y
Cuts at: 1132 1229 2788 3546 3598 5414 6959 1132
Size: 97 1559 758 52 1816 1545 2229
Fragments arranged by size:
2229 1816 1559 1545 758 97 52
PasI CC'CwG_GG
Cuts at: 1348 3115 4058 4771 1348
Size: 1767 943 713 4633
Fragments arranged by size:
4633 1767 943 713
PciI A'CATG_T
Cuts at: 6955 6955
Size: 8056
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2081 4064 5263 1108
Size: 973 1983 1199 3901
Fragments arranged by size:
3901 1983 1199 973
PfoI T'CCnGG_A
Cuts at: 3849 3849
Size: 8056
PmeI GTTT'AAAC
Cuts at: 5360 5360
Size: 8056
PmlI CAC'GTG
Cuts at: 4545 4545
Size: 8056
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6125 6157 7638 7670 6125
Size: 32 1481 32 6511
Fragments arranged by size:
6511 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3064 3277 3474 4657 3064
Size: 213 197 1183 6463
Fragments arranged by size:
6463 1183 213 197
PshAI GACnn'nnGTC
Cuts at: 1613 3332 1613
Size: 1719 6337
PsiI TTA'TAA
Cuts at: 5626 5626
Size: 8056
PspOMI G'GGCC_C
Cuts at: 5165 5165
Size: 8056
PspXI vC'TCGA_Gb
Cuts at: 2242 2242
Size: 8056
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2048 2080 2048
Size: 32 8024
PstI C_TGCA'G
Cuts at: 830 2760 2949 3045 4767 5408 830
Size: 1930 189 96 1722 641 3478
Fragments arranged by size:
3478 1930 1722 641 189 96
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8056
PvuII CAG'CTG
Cuts at: 2554 2944 3106 3223 3415 3814 3973 5132 5203
Size: 390 162 117 192 399 159 1159 71
Cuts at: 5203 2554
Size: 5407
Fragments arranged by size:
5407 1159 399 390 192 162 159 117
71
SacI G_AGCT'C
Cuts at: 3269 3320 4964 3269
Size: 51 1644 6361
Fragments arranged by size:
6361 1644 51
SalI G'TCGA_C
Cuts at: 1936 5398 1936
Size: 3462 4594
SanDI GG'GwC_CC
Cuts at: 3064 3474 3064
Size: 410 7646
SapI GCTCTTCn'nnn_
Cuts at: 2670 3929 2670
Size: 1259 6797
SbfI CC_TGCA'GG
Cuts at: 4767 5408 4767
Size: 641 7415
ScaI AGT'ACT
Cuts at: 3134 4535 6304 8016 3134
Size: 1401 1769 1712 3174
Fragments arranged by size:
3174 1769 1712 1401
SexAI A'CCwGG_T
Cuts at: 4878 4878
Size: 8056
SfcI C'TryA_G
Cuts at: 826 1045 2756 2939 2945 3041 3160 4077 4763
Size: 219 1711 183 6 96 119 917 686
Cuts at: 4763 5404 6539 7220 7411 826
Size: 641 1135 681 191 1471
Fragments arranged by size:
1711 1471 1135 917 686 681 641 219
191 183 119 96 6
SfoI GGC'GCC
Cuts at: 3793 3961 5178 3793
Size: 168 1217 6671
Fragments arranged by size:
6671 1217 168
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8056
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8056
SmaI CCC'GGG
Cuts at: 1872 3332 5383 1872
Size: 1460 2051 4545
Fragments arranged by size:
4545 2051 1460
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2178 2242 3689 4108 6160 7061
Size: 197 926 235 64 1447 419 2052 901
Cuts at: 7061 7323 820
Size: 262 1553
Fragments arranged by size:
2052 1553 1447 926 901 419 262 235
197 64
SnaBI TAC'GTA
Cuts at: 493 5847 493
Size: 5354 2702
SpeI A'CTAG_T
Cuts at: 152 5353 152
Size: 5201 2855
SphI G_CATG'C
Cuts at: 1132 5414 1132
Size: 4282 3774
SspI AAT'ATT
Cuts at: 5 52 5980 5
Size: 47 5928 2081
Fragments arranged by size:
5928 2081 47
StuI AGG'CCT
Cuts at: 1517 2153 1517
Size: 636 7420
StyI C'CwwG_G
Cuts at: 513 1065 2004 2044 2224 2571 2689 3271 4887
Size: 552 939 40 180 347 118 582 1616
Cuts at: 4887 5495 513
Size: 608 3074
Fragments arranged by size:
3074 1616 939 608 582 552 347 180
118 40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 1997 2459 3875 3904 4949 6083 6100
Size: 1255 417 462 1416 29 1045 1134 17
Cuts at: 6100 6253 6438 6883 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1416 1255 1134 1045 462 445 417
185 153 29 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2022 3132 4489
Size: 274 80 33 51 1443 45 1110 1357
Cuts at: 4489 4533 6302 8014 96
Size: 44 1769 1712 138
Fragments arranged by size:
1769 1712 1443 1357 1110 274 138 80
51 45 44 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2070 2102 2577 2609 3084 3116 3584 3616 4713
Size: 32 475 32 475 32 468 32 1097
Cuts at: 4713 4745 8016 8048 2070
Size: 32 3271 32 2078
Fragments arranged by size:
3271 2078 1097 475 475 468 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 5249 5252 1316
Size: 3933 3 4120
Fragments arranged by size:
4120 3933 3
XbaI T'CTAG_A
Cuts at: 5392 5392
Size: 8056
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2042 5227 2042
Size: 3185 4871
XhoI C'TCGA_G
Cuts at: 1943 2242 1943
Size: 299 7757
XmaI C'CCGG_G
Cuts at: 1870 3330 5381 1870
Size: 1460 2051 4545
Fragments arranged by size:
4545 2051 1460
XmnI GAAnn'nnTTC
Cuts at: 2060 3563 3713 6185 2060
Size: 1503 150 2472 3931
Fragments arranged by size:
3931 2472 1503 150
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5251 5864 276
Size: 53 83 186 936 3717 613 2468
Fragments arranged by size:
3717 2468 936 613 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BfrBI BplI BsiWI BsmBI
DraIII FalI FseI FspAI NaeI NgoMIV NruI NsiI
PacI RsrII SacII SfiI SrfI SwaI