Restriction map for
pFN21AB3451
(Circular) (Six-base) MAPSORT of: pFN21AB3451.seq Check: 2607 from: 1 to: 5497
pFN21AB3451 5497 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2119 2119
Size: 5497
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3307 278
Size: 53 83 186 936 1771 2468
Fragments arranged by size:
2468 1771 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2840 1937
Size: 903 4594
Acc65I G'GTAC_C
Cuts at: 2818 2818
Size: 5497
AclI AA'CG_TT
Cuts at: 1563 3624 3997 1563
Size: 2061 373 3063
Fragments arranged by size:
3063 2061 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2289 2678 3560 4944 5212 1632
Size: 657 389 882 1384 268 1917
Fragments arranged by size:
1917 1384 882 657 389 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5300
AflIII A'CryG_T
Cuts at: 1203 4396 5373 1203
Size: 3193 977 1327
Fragments arranged by size:
3193 1327 977
AgeI A'CCGG_T
Cuts at: 4328 4328
Size: 5497
AhdI GACnn_n'nnGTC
Cuts at: 4226 4226
Size: 5497
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5497
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5497
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2766 2798 2766
Size: 32 5465
AlwNI CAG_nnn'CTG
Cuts at: 3240 4812 3240
Size: 1572 3925
ApaLI G'TGCA_C
Cuts at: 3554 4710 3554
Size: 1156 4341
ApoI r'AATT_y
Cuts at: 1493 2252 2806 3032 3184 5293 1493
Size: 759 554 226 152 2109 1697
Fragments arranged by size:
2109 1697 759 554 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4051 160
Size: 872 3019 1606
Fragments arranged by size:
3019 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5497
AvaI C'yCGr_G
Cuts at: 1870 1943 2523 2822 1870
Size: 73 580 299 4545
Fragments arranged by size:
4545 580 299 73
BamHI G'GATC_C
Cuts at: 2827 3194 2827
Size: 367 5130
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2818 4273 618
Size: 325 217 627 1031 1455 1842
Fragments arranged by size:
1842 1455 1031 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2816 1391
Size: 1425 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2465 2626 928
Size: 971 566 161 3799
Fragments arranged by size:
3799 971 566 161
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2726 2760 3688 3722 1903
Size: 34 789 34 928 34 3678
Fragments arranged by size:
3678 928 789 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3389 4599 3389
Size: 1210 4287
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5497
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4108 136
Size: 107 122 71 631 605 2436 1525
Fragments arranged by size:
2436 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5492 5492
Size: 5497
BlpI GC'TnA_GC
Cuts at: 2472 2914 2472
Size: 442 5055
Bme1580I G_kGCm'C
Cuts at: 1790 3558 4714 1790
Size: 1768 1156 2573
Fragments arranged by size:
2573 1768 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5497
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2739 3228 4186
Size: 416 53 83 399 24 1322 489 958
Cuts at: 4186 442
Size: 1753
Fragments arranged by size:
1753 1322 958 489 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5497
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4157 1239
Size: 2918 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2174 3622 4487 4785 2174
Size: 1448 865 298 2886
Fragments arranged by size:
2886 1448 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2192 4160 882
Size: 617 693 1968 2219
Fragments arranged by size:
2219 1968 693 617
BsaAI yAC'GTr
Cuts at: 493 1204 3288 493
Size: 711 2084 2702
Fragments arranged by size:
2702 2084 711
BsaBI GATnn'nnATC
Cuts at: 2513 5491 2513
Size: 2978 2519
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3304 3686 4321 275
Size: 53 83 186 936 1771 382 635 1451
Fragments arranged by size:
1771 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2496 3930 4328 4602 4749 5160 2496
Size: 1434 398 274 147 411 2833
Fragments arranged by size:
2833 1434 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5272
Fragments arranged by size:
5272 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5497
BseYI C'CCAG_C
Cuts at: 4700 5196 4700
Size: 496 5001
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2552 1163
Size: 573 816 4108
Fragments arranged by size:
4108 816 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2947 3708 4736 1540
Size: 275 186 946 761 1028 2301
Fragments arranged by size:
2301 1028 946 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3558 3643 4714 3558
Size: 85 1071 4341
Fragments arranged by size:
4341 1071 85
BsmI GAATG_Cn'
Cuts at: 3008 3101 3008
Size: 93 5404
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2816 3558 3643 4714 1391
Size: 399 1026 742 85 1071 2174
Fragments arranged by size:
2174 1071 1026 742 399 85
BspEI T'CCGG_A
Cuts at: 2496 5160 2496
Size: 2664 2833
BspHI T'CATG_A
Cuts at: 2517 3386 5116 2517
Size: 869 1730 2898
Fragments arranged by size:
2898 1730 869
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2119 2852 844
Size: 1275 733 3489
Fragments arranged by size:
3489 1275 733
BsrBI CCG'CTC
Cuts at: 1394 1823 3384 1394
Size: 429 1561 3507
Fragments arranged by size:
3507 1561 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3992 4166 65
Size: 1183 2744 174 1396
Fragments arranged by size:
2744 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4141 4328 1700
Size: 110 2331 187 2869
Fragments arranged by size:
2869 2331 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5497
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5497
BssSI C'ACGA_G
Cuts at: 3557 4569 3557
Size: 1012 4485
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5497
BstBI TT'CG_AA
Cuts at: 4301 4301
Size: 5497
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5497
BstYI r'GATC_y
Cuts at: 1972 2314 2827 3194 3579 3596 5048 5134 5146
Size: 342 513 367 385 17 1452 86 12
Cuts at: 5146 5492 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 513 385 367 346 342 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2305 2340 513
Size: 552 939 301 35 3670
Fragments arranged by size:
3670 939 552 301 35
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4758
Fragments arranged by size:
4758 683 56
BtsI GCAGTG_nn'
Cuts at: 3013 3807 3827 3013
Size: 794 20 4683
Fragments arranged by size:
4683 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5497
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5462
DraI TTT'AAA
Cuts at: 2801 2801
Size: 5497
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4504 809
Size: 507 3188 1802
Fragments arranged by size:
3188 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2944 3833 5462
Size: 54 1425 50 275 18 1114 889 1629
Cuts at: 5462 8
Size: 43
Fragments arranged by size:
1629 1425 1114 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2944 1537
Size: 275 1132 4090
Fragments arranged by size:
4090 1132 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3427 1353
Size: 2074 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2477 4084 4458 4604 387
Size: 1064 138 888 1607 374 146 1280
Fragments arranged by size:
1607 1280 1064 888 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2289 2678 3560 4157 4944 5212 1239
Size: 393 657 389 882 597 787 268 1524
Fragments arranged by size:
1524 882 787 657 597 393 389 268
EcoO109I rG'GnC_Cy
Cuts at: 2157 2157
Size: 5497
EcoRI G'AATT_C
Cuts at: 2806 3184 2806
Size: 378 5119
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2231 2231
Size: 5497
FspI TGC'GCA
Cuts at: 4003 4003
Size: 5497
HaeII r_GCGC'y
Cuts at: 1678 2029 4644 1678
Size: 351 2615 2531
Fragments arranged by size:
2615 2531 351
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2356 2388 4138 4170 4212
Size: 32 135 32 784 32 1750 32 42
Cuts at: 4212 4244 1373
Size: 32 2626
Fragments arranged by size:
2626 1750 784 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2841 3087 669
Size: 988 281 903 246 3079
Fragments arranged by size:
3079 988 903 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5497
HpaI GTT'AAC
Cuts at: 3087 3087
Size: 5497
KpnI G_GTAC'C
Cuts at: 2822 2822
Size: 5497
MfeI C'AATT_G
Cuts at: 3096 3096
Size: 5497
MluI A'CGCG_T
Cuts at: 5373 5373
Size: 5497
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4611 4795 1305
Size: 3306 184 2007
Fragments arranged by size:
3306 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3675
Fragments arranged by size:
3675 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2379 3455 3814 3973 518
Size: 648 1213 1076 359 159 2042
Fragments arranged by size:
2042 1213 1076 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2913 3590 4738 4983 1606
Size: 1307 677 1148 245 2120
Fragments arranged by size:
2120 1307 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2305 2340 513
Size: 552 939 301 35 3670
Fragments arranged by size:
3670 939 552 301 35
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5497
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5497
NotI GC'GGCC_GC
Cuts at: 2944 2944
Size: 5497
NspI r_CATG'y
Cuts at: 1132 1229 2855 4400 1132
Size: 97 1626 1545 2229
Fragments arranged by size:
2229 1626 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5497
PciI A'CATG_T
Cuts at: 4396 4396
Size: 5497
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2311 1108
Size: 1203 4294
PmeI GTTT'AAAC
Cuts at: 2801 2801
Size: 5497
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3566 3598 5079 5111 3566
Size: 32 1481 32 3952
Fragments arranged by size:
3952 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2157 2157
Size: 5497
PshAI GACnn'nnGTC
Cuts at: 1613 2147 1613
Size: 534 4963
PsiI TTA'TAA
Cuts at: 3067 3067
Size: 5497
PstI C_TGCA'G
Cuts at: 830 2849 830
Size: 2019 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5497
SalI G'TCGA_C
Cuts at: 1936 2839 1936
Size: 903 4594
SbfI CC_TGCA'GG
Cuts at: 2849 2849
Size: 5497
ScaI AGT'ACT
Cuts at: 3745 5457 3745
Size: 1712 3785
SfcI C'TryA_G
Cuts at: 826 1045 2845 3980 4661 4852 826
Size: 219 1800 1135 681 191 1471
Fragments arranged by size:
1800 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5497
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5497
SmaI CCC'GGG
Cuts at: 1872 2824 1872
Size: 952 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2189 3601 4502 4764 820
Size: 197 926 246 1412 901 262 1553
Fragments arranged by size:
1553 1412 926 901 262 246 197
SnaBI TAC'GTA
Cuts at: 493 3288 493
Size: 2795 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5497
SphI G_CATG'C
Cuts at: 1132 2855 1132
Size: 1723 3774
SspI AAT'ATT
Cuts at: 5 52 2134 3421 5
Size: 47 2082 1287 2081
Fragments arranged by size:
2082 2081 1287 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5497
StyI C'CwwG_G
Cuts at: 513 1065 2004 2161 2305 2340 2936 513
Size: 552 939 157 144 35 596 3074
Fragments arranged by size:
3074 939 596 552 157 144 35
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3524 3541 3694 3879 4324 325
Size: 1255 1944 17 153 185 445 1498
Fragments arranged by size:
1944 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3743 5455 96
Size: 274 80 33 51 1443 1766 1712 138
Fragments arranged by size:
1766 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5457 5489 5457
Size: 32 5465
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5497
XbaI T'CTAG_A
Cuts at: 2833 2833
Size: 5497
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2062 2674 2062
Size: 612 4885
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5497
XmaI C'CCGG_G
Cuts at: 1870 2822 1870
Size: 952 4545
XmnI GAAnn'nnTTC
Cuts at: 2274 3626 2274
Size: 1352 4145
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3305 276
Size: 53 83 186 936 1771 2468
Fragments arranged by size:
2468 1771 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI