Restriction map for
pFN21AB3450
(Circular) (Six-base) MAPSORT of: pFN21AB3450.seq Check: 5883 from: 1 to: 5584
pFN21AB3450 5584 bp
With 182 enzymes: SgfI *
October 20, 2009 10:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3394 278
Size: 53 83 186 936 1858 2468
Fragments arranged by size:
2468 1858 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2927 1937
Size: 990 4594
Acc65I G'GTAC_C
Cuts at: 2905 2905
Size: 5584
AclI AA'CG_TT
Cuts at: 1563 3711 4084 1563
Size: 2148 373 3063
Fragments arranged by size:
3063 2148 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3647 5031 5299 1632
Size: 2015 1384 268 1917
Fragments arranged by size:
2015 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5387
AflIII A'CryG_T
Cuts at: 1203 4483 5460 1203
Size: 3280 977 1327
Fragments arranged by size:
3280 1327 977
AgeI A'CCGG_T
Cuts at: 4415 4415
Size: 5584
AhdI GACnn_n'nnGTC
Cuts at: 4313 4313
Size: 5584
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5584
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2370 1184
Size: 1186 4398
AlwNI CAG_nnn'CTG
Cuts at: 3327 4899 3327
Size: 1572 4012
ApaI G_GGCC'C
Cuts at: 2037 2045 2037
Size: 8 5576
ApaLI G'TGCA_C
Cuts at: 3641 4797 3641
Size: 1156 4428
ApoI r'AATT_y
Cuts at: 1493 2893 3119 3271 5380 1493
Size: 1400 226 152 2109 1697
Fragments arranged by size:
2109 1697 1400 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4138 160
Size: 872 3106 1606
Fragments arranged by size:
3106 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5584
AvaI C'yCGr_G
Cuts at: 1870 1943 2036 2682 2909 1870
Size: 73 93 646 227 4545
Fragments arranged by size:
4545 646 227 93 73
BamHI G'GATC_C
Cuts at: 2914 3281 2914
Size: 367 5217
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2905 4360 618
Size: 325 217 627 1118 1455 1842
Fragments arranged by size:
1842 1455 1118 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2037 2045 2155 2903 1391
Size: 646 8 110 748 4072
Fragments arranged by size:
4072 748 646 110 8
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4613
BbvCI CC'TCA_GC
Cuts at: 2677 2677
Size: 5584
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2596 2630 3775 3809 1903
Size: 34 659 34 1145 34 3678
Fragments arranged by size:
3678 1145 659 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3476 4686 3476
Size: 1210 4374
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5584
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2049 2568 4195
Size: 107 122 71 631 605 377 519 1627
Cuts at: 4195 136
Size: 1525
Fragments arranged by size:
1627 1525 631 605 519 377 122 107
71
BglII A'GATC_T
Cuts at: 2244 5579 2244
Size: 3335 2249
BlpI GC'TnA_GC
Cuts at: 3001 3001
Size: 5584
Bme1580I G_kGCm'C
Cuts at: 1790 2037 2045 2609 3645 4801 1790
Size: 247 8 564 1036 1156 2573
Fragments arranged by size:
2573 1156 1036 564 247 8
BmgBI CAC'GTC
Cuts at: 1318 2413 1318
Size: 1095 4489
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2521 3315 4273
Size: 416 53 83 399 24 1104 794 958
Cuts at: 4273 442
Size: 1753
Fragments arranged by size:
1753 1104 958 794 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5584
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2258 2850 4244 1239
Size: 1019 592 1394 2579
Fragments arranged by size:
2579 1394 1019 592
Bpu10I CC'TnA_GC
Cuts at: 2677 2677
Size: 5584
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2294 3709 4574 4872 2294
Size: 1415 865 298 3006
Fragments arranged by size:
3006 1415 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4247 882
Size: 617 2748 2219
Fragments arranged by size:
2748 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3375 493
Size: 711 2171 2702
Fragments arranged by size:
2702 2171 711
BsaBI GATnn'nnATC
Cuts at: 5578 5578
Size: 5584
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2016 3391 3773 4408
Size: 53 83 186 936 483 1375 382 635
Cuts at: 4408 275
Size: 1451
Fragments arranged by size:
1451 1375 936 635 483 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4017 4415 4689 4836 5247 4017
Size: 398 274 147 411 4354
Fragments arranged by size:
4354 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2779 2809 2848 2878 1178
Size: 30 165 30 1376 30 39 30 3884
Fragments arranged by size:
3884 1376 165 39 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2255 1186
Size: 1069 4515
BseYI C'CCAG_C
Cuts at: 4787 5283 4787
Size: 496 5088
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2043 2360 2580 2674 2870 1163
Size: 573 307 317 220 94 196 3877
Fragments arranged by size:
3877 573 317 307 220 196 94
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2122 3034 3795 4823 1540
Size: 275 186 121 912 761 1028 2301
Fragments arranged by size:
2301 1028 912 761 275 186 121
BsiHKAI G_wGCw'C
Cuts at: 2155 2435 3645 3730 4801 2155
Size: 280 1210 85 1071 2938
Fragments arranged by size:
2938 1210 1071 280 85
BsmI GAATG_Cn'
Cuts at: 3095 3188 3095
Size: 93 5491
BsmBI CGTCTCn'nnnn_
Cuts at: 2623 2623
Size: 5584
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2037 2045 2155 2435 2609 2903 3645
Size: 399 247 8 110 280 174 294 742
Cuts at: 3645 3730 4801 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 294 280 247 174
110 85 8
BspEI T'CCGG_A
Cuts at: 5247 5247
Size: 5584
BspHI T'CATG_A
Cuts at: 2646 3473 5203 2646
Size: 827 1730 3027
Fragments arranged by size:
3027 1730 827
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2939 844
Size: 2095 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3471 1394
Size: 429 1648 3507
Fragments arranged by size:
3507 1648 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4079 4253 65
Size: 1183 2831 174 1396
Fragments arranged by size:
2831 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2018 2357 4228 4415 1700
Size: 110 208 339 1871 187 2869
Fragments arranged by size:
2869 1871 339 208 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5584
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5584
BssSI C'ACGA_G
Cuts at: 3644 4656 3644
Size: 1012 4572
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2387 794
Size: 1593 3991
BstBI TT'CG_AA
Cuts at: 4388 4388
Size: 5584
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5584
BstXI CCAn_nnnn'nTGG
Cuts at: 2443 2473 2443
Size: 30 5554
BstYI r'GATC_y
Cuts at: 1972 2244 2914 3281 3666 3683 5135 5221 5233
Size: 272 670 367 385 17 1452 86 12
Cuts at: 5233 5579 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 670 385 367 346 272 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2068 2080 2122 2146 2458 513
Size: 552 939 64 12 42 24 312 3639
Fragments arranged by size:
3639 939 552 312 64 42 24 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4845
Fragments arranged by size:
4845 683 56
BtsI GCAGTG_nn'
Cuts at: 2101 2691 3100 3894 3914 2101
Size: 590 409 794 20 3771
Fragments arranged by size:
3771 794 590 409 20
ClaI AT'CG_AT
Cuts at: 1554 2661 1554
Size: 1107 4477
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5549
DraI TTT'AAA
Cuts at: 2888 2888
Size: 5584
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2822 4591 809
Size: 507 1506 1769 1802
Fragments arranged by size:
1802 1769 1506 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2119 2359 2407
Size: 54 1425 50 275 18 289 240 48
Cuts at: 2407 2445 2475 3031 3920 5549 8
Size: 38 30 556 889 1629 43
Fragments arranged by size:
1629 1425 889 556 289 275 240 54
50 48 43 38 30 18
EagI C'GGCC_G
Cuts at: 1537 1812 2119 3031 1537
Size: 275 307 912 4090
Fragments arranged by size:
4090 912 307 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2258 3514 1353
Size: 905 1256 3423
Fragments arranged by size:
3423 1256 905
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 4171 4545 4691 387
Size: 1064 138 435 2147 374 146 1280
Fragments arranged by size:
2147 1280 1064 435 374 146 138
EcoICRI GAG'CTC
Cuts at: 2153 2153
Size: 5584
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2258 2850 3647 4244 5031 5299 1239
Size: 393 626 592 797 597 787 268 1524
Fragments arranged by size:
1524 797 787 626 597 592 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2771 2771
Size: 5584
EcoO109I rG'GnC_Cy
Cuts at: 2033 2041 2042 2301 2033
Size: 8 1 259 5316
Fragments arranged by size:
5316 259 8 1
EcoRI G'AATT_C
Cuts at: 2893 3271 2893
Size: 378 5206
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2740 2740
Size: 5584
FspI TGC'GCA
Cuts at: 2501 4090 2501
Size: 1589 3995
HaeII r_GCGC'y
Cuts at: 1678 4731 1678
Size: 3053 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2848 2880 4225 4257 4299
Size: 32 135 32 1276 32 1345 32 42
Cuts at: 4299 4331 1373
Size: 32 2626
Fragments arranged by size:
2626 1345 1276 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2928 3174 669
Size: 988 281 990 246 3079
Fragments arranged by size:
3079 990 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5584
HpaI GTT'AAC
Cuts at: 3174 3174
Size: 5584
KpnI G_GTAC'C
Cuts at: 2909 2909
Size: 5584
MfeI C'AATT_G
Cuts at: 3183 3183
Size: 5584
MluI A'CGCG_T
Cuts at: 5460 5460
Size: 5584
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4698 4882 1305
Size: 3393 184 2007
Fragments arranged by size:
3393 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2409 2447 2477 10
Size: 54 1425 343 577 38 30 3117
Fragments arranged by size:
3117 1425 577 343 54 38 30
MslI CAynn'nnrTG
Cuts at: 518 1166 3542 3901 4060 518
Size: 648 2376 359 159 2042
Fragments arranged by size:
2376 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2027 2070 2082 2148 2565 2854 2870 3000
Size: 421 43 12 66 417 289 16 130
Cuts at: 3000 3677 4825 5070 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 421 417 289 245 130
66 43 16 12
NaeI GCC'GGC
Cuts at: 2359 2359
Size: 5584
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4093
Fragments arranged by size:
4093 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5584
NgoMIV G'CCGG_C
Cuts at: 2357 2357
Size: 5584
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5584
NotI GC'GGCC_GC
Cuts at: 3031 3031
Size: 5584
NspI r_CATG'y
Cuts at: 1132 1229 2942 4487 1132
Size: 97 1713 1545 2229
Fragments arranged by size:
2229 1713 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2054 2601 2701 1348
Size: 706 547 100 4231
Fragments arranged by size:
4231 706 547 100
PciI A'CATG_T
Cuts at: 4483 4483
Size: 5584
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5584
PmeI GTTT'AAAC
Cuts at: 2888 2888
Size: 5584
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3653 3685 5166 5198 3653
Size: 32 1481 32 4039
Fragments arranged by size:
4039 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5584
PsiI TTA'TAA
Cuts at: 3154 3154
Size: 5584
PspOMI G'GGCC_C
Cuts at: 2033 2041 2033
Size: 8 5576
PstI C_TGCA'G
Cuts at: 830 2936 830
Size: 2106 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5584
PvuII CAG'CTG
Cuts at: 2870 2870
Size: 5584
SacI G_AGCT'C
Cuts at: 2155 2155
Size: 5584
SacII CC_GC'GG
Cuts at: 2071 2083 2149 2071
Size: 12 66 5506
Fragments arranged by size:
5506 66 12
SalI G'TCGA_C
Cuts at: 1936 2926 1936
Size: 990 4594
SbfI CC_TGCA'GG
Cuts at: 2936 2936
Size: 5584
ScaI AGT'ACT
Cuts at: 3832 5544 3832
Size: 1712 3872
SexAI A'CCwGG_T
Cuts at: 2369 2369
Size: 5584
SfcI C'TryA_G
Cuts at: 826 1045 2746 2932 4067 4748 4939 826
Size: 219 1701 186 1135 681 191 1471
Fragments arranged by size:
1701 1471 1135 681 219 191 186
SfiI GGCCn_nnn'nGGCC
Cuts at: 2049 2049
Size: 5584
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5584
SgrAI Cr'CCGG_yG
Cuts at: 1700 2018 1700
Size: 318 5266
SmaI CCC'GGG
Cuts at: 1872 2038 2684 2911 1872
Size: 166 646 227 4545
Fragments arranged by size:
4545 646 227 166
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2309 3688 4589 4851 820
Size: 197 926 366 1379 901 262 1553
Fragments arranged by size:
1553 1379 926 901 366 262 197
SnaBI TAC'GTA
Cuts at: 493 3375 493
Size: 2882 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5584
SphI G_CATG'C
Cuts at: 1132 2942 1132
Size: 1810 3774
SspI AAT'ATT
Cuts at: 5 52 3508 5
Size: 47 3456 2081
Fragments arranged by size:
3456 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5584
StyI C'CwwG_G
Cuts at: 513 1065 2004 2436 2715 3023 513
Size: 552 939 432 279 308 3074
Fragments arranged by size:
3074 939 552 432 308 279
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2405 2411 2614 3611 3628 3781 3966
Size: 1255 825 6 203 997 17 153 185
Cuts at: 3966 4411 325
Size: 445 1498
Fragments arranged by size:
1498 1255 997 825 445 203 185 153
17 6
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3830 5542 96
Size: 274 80 33 51 1443 1853 1712 138
Fragments arranged by size:
1853 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2056 2088 2165 2197 2779 2811 5544 5576 2056
Size: 32 77 32 582 32 2733 32 2064
Fragments arranged by size:
2733 2064 582 77 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5584
XbaI T'CTAG_A
Cuts at: 2920 2920
Size: 5584
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5584
XmaI C'CCGG_G
Cuts at: 1870 2036 2682 2909 1870
Size: 166 646 227 4545
Fragments arranged by size:
4545 646 227 166
XmnI GAAnn'nnTTC
Cuts at: 3713 3713
Size: 5584
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3392 276
Size: 53 83 186 936 1858 2468
Fragments arranged by size:
2468 1858 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SacII SalI SbfI ScaI SexAI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BstZ17I Bsu36I DraIII EcoRV FseI FspAI
KasI NarI NruI NsiI PacI PfoI PmlI PpuMI
PspXI PsrI RsrII SanDI SapI SfoI SrfI SwaI
XcmI