Restriction map for
pFN21AB3449
(Circular) (Six-base) MAPSORT of: pFN21AB3449.seq Check: 6254 from: 1 to: 5302
pFN21AB3449 5302 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3112 278
Size: 53 83 186 936 1576 2468
Fragments arranged by size:
2468 1576 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2645 1937
Size: 708 4594
Acc65I G'GTAC_C
Cuts at: 2623 2623
Size: 5302
AclI AA'CG_TT
Cuts at: 1563 3429 3802 1563
Size: 1866 373 3063
Fragments arranged by size:
3063 1866 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 1995 3365 4749 5017 1632
Size: 363 1370 1384 268 1917
Fragments arranged by size:
1917 1384 1370 363 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5105
AflIII A'CryG_T
Cuts at: 1203 4201 5178 1203
Size: 2998 977 1327
Fragments arranged by size:
2998 1327 977
AgeI A'CCGG_T
Cuts at: 4133 4133
Size: 5302
AhdI GACnn_n'nnGTC
Cuts at: 4031 4031
Size: 5302
AleI CACnn'nnGTG
Cuts at: 1166 2153 1166
Size: 987 4315
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5302
AlwNI CAG_nnn'CTG
Cuts at: 3045 4617 3045
Size: 1572 3730
ApaLI G'TGCA_C
Cuts at: 3359 4515 3359
Size: 1156 4146
ApoI r'AATT_y
Cuts at: 1493 2198 2611 2837 2989 5098 1493
Size: 705 413 226 152 2109 1697
Fragments arranged by size:
2109 1697 705 413 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3856 160
Size: 872 2824 1606
Fragments arranged by size:
2824 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5302
AvaI C'yCGr_G
Cuts at: 1870 1943 2627 1870
Size: 73 684 4545
Fragments arranged by size:
4545 684 73
BamHI G'GATC_C
Cuts at: 2632 2999 2632
Size: 367 4935
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2031 2623 4078 618
Size: 325 217 627 244 592 1455 1842
Fragments arranged by size:
1842 1455 627 592 325 244 217
BanII G_rGCy'C
Cuts at: 1391 2621 1391
Size: 1230 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2001 928
Size: 971 102 4229
Fragments arranged by size:
4229 971 102
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3493 3527 1903
Size: 34 1556 34 3678
Fragments arranged by size:
3678 1556 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3194 4404 3194
Size: 1210 4092
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5302
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2529 3913 136
Size: 107 122 71 631 605 857 1384 1525
Fragments arranged by size:
1525 1384 857 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5297 5297
Size: 5302
BlpI GC'TnA_GC
Cuts at: 2719 2719
Size: 5302
Bme1580I G_kGCm'C
Cuts at: 1790 2034 2162 2525 3363 4519 1790
Size: 244 128 363 838 1156 2573
Fragments arranged by size:
2573 1156 838 363 244 128
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5302
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2303 3033 3991
Size: 416 53 83 399 24 886 730 958
Cuts at: 3991 442
Size: 1753
Fragments arranged by size:
1753 958 886 730 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5302
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3962 1239
Size: 2723 2579
Bpu10I CC'TnA_GC
Cuts at: 2343 2343
Size: 5302
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3427 4292 4590 3427
Size: 865 298 4139
Fragments arranged by size:
4139 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3965 882
Size: 617 2466 2219
Fragments arranged by size:
2466 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3093 493
Size: 711 1889 2702
Fragments arranged by size:
2702 1889 711
BsaBI GATnn'nnATC
Cuts at: 5296 5296
Size: 5302
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3109 3491 4126 275
Size: 53 83 186 936 1576 382 635 1451
Fragments arranged by size:
1576 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3735 4133 4407 4554 4965 3735
Size: 398 274 147 411 4072
Fragments arranged by size:
4072 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5077
Fragments arranged by size:
5077 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5302
BseYI C'CCAG_C
Cuts at: 2524 4505 5001 2524
Size: 1981 496 2825
Fragments arranged by size:
2825 1981 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4729
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2752 3513 4541 1540
Size: 275 186 751 761 1028 2301
Fragments arranged by size:
2301 1028 761 751 275 186
BsiHKAI G_wGCw'C
Cuts at: 3363 3448 4519 3363
Size: 85 1071 4146
Fragments arranged by size:
4146 1071 85
BsmI GAATG_Cn'
Cuts at: 2813 2906 2813
Size: 93 5209
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2034 2162 2525 2621 3363 3448 4519
Size: 399 244 128 363 96 742 85 1071
Cuts at: 4519 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 363 244 128 96
85
BspEI T'CCGG_A
Cuts at: 4965 4965
Size: 5302
BspHI T'CATG_A
Cuts at: 3191 4921 3191
Size: 1730 3572
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2657 844
Size: 1813 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2457 3189 1394
Size: 429 634 732 3507
Fragments arranged by size:
3507 732 634 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2172 2337 3797 3971 65
Size: 1183 924 165 1460 174 1396
Fragments arranged by size:
1460 1396 1183 924 174 165
BsrFI r'CCGG_y
Cuts at: 1700 1810 3946 4133 1700
Size: 110 2136 187 2869
Fragments arranged by size:
2869 2136 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5302
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5302
BssSI C'ACGA_G
Cuts at: 3362 4374 3362
Size: 1012 4290
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5302
BstBI TT'CG_AA
Cuts at: 4106 4106
Size: 5302
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5302
BstXI CCAn_nnnn'nTGG
Cuts at: 2225 2532 2225
Size: 307 4995
BstYI r'GATC_y
Cuts at: 1972 2632 2999 3384 3401 4853 4939 4951 5297
Size: 660 367 385 17 1452 86 12 346
Cuts at: 5297 1972
Size: 1977
Fragments arranged by size:
1977 1452 660 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2163 513
Size: 552 1098 3652
Fragments arranged by size:
3652 1098 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4563
Fragments arranged by size:
4563 683 56
BtsI GCAGTG_nn'
Cuts at: 2551 2818 3612 3632 2551
Size: 267 794 20 4221
Fragments arranged by size:
4221 794 267 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5302
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5267
DraI TTT'AAA
Cuts at: 2083 2606 2083
Size: 523 4779
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4309 809
Size: 507 2993 1802
Fragments arranged by size:
2993 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2452 2749 3638
Size: 54 1425 50 275 18 622 297 889
Cuts at: 3638 5267 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 622 297 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2749 1537
Size: 275 937 4090
Fragments arranged by size:
4090 937 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3232 1353
Size: 1879 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3889 4263 4409 387
Size: 1064 138 2300 374 146 1280
Fragments arranged by size:
2300 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 1995 3365 3962 4749 5017 1239
Size: 393 363 1370 597 787 268 1524
Fragments arranged by size:
1524 1370 787 597 393 363 268
EcoO109I rG'GnC_Cy
Cuts at: 2297 2389 2297
Size: 92 5210
EcoRI G'AATT_C
Cuts at: 2611 2989 2611
Size: 378 4924
EcoRV GAT'ATC
Cuts at: 2089 2089
Size: 5302
FspI TGC'GCA
Cuts at: 3808 3808
Size: 5302
HaeII r_GCGC'y
Cuts at: 1678 4449 1678
Size: 2771 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3943 3975 4017 4049 1373
Size: 32 135 32 2371 32 42 32 2626
Fragments arranged by size:
2626 2371 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2646 2892 669
Size: 988 281 708 246 3079
Fragments arranged by size:
3079 988 708 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5302
HpaI GTT'AAC
Cuts at: 2892 2892
Size: 5302
KpnI G_GTAC'C
Cuts at: 2627 2627
Size: 5302
MfeI C'AATT_G
Cuts at: 2901 2901
Size: 5302
MluI A'CGCG_T
Cuts at: 5178 5178
Size: 5302
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4416 4600 1305
Size: 3111 184 2007
Fragments arranged by size:
3111 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3480
Fragments arranged by size:
3480 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2153 3260 3619 3778 518
Size: 648 987 1107 359 159 2042
Fragments arranged by size:
2042 1107 987 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2060 2528 2718 3395 4543 4788 1606
Size: 454 468 190 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 468 454 245 190
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4750
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5302
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5302
NotI GC'GGCC_GC
Cuts at: 2749 2749
Size: 5302
NspI r_CATG'y
Cuts at: 1132 1229 2660 4205 1132
Size: 97 1431 1545 2229
Fragments arranged by size:
2229 1545 1431 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5302
PciI A'CATG_T
Cuts at: 4201 4201
Size: 5302
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5302
PmeI GTTT'AAAC
Cuts at: 2606 2606
Size: 5302
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3371 3403 4884 4916 3371
Size: 32 1481 32 3757
Fragments arranged by size:
3757 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2297 2297
Size: 5302
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5302
PsiI TTA'TAA
Cuts at: 2872 2872
Size: 5302
PstI C_TGCA'G
Cuts at: 830 2654 830
Size: 1824 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5302
PvuII CAG'CTG
Cuts at: 2060 2528 2060
Size: 468 4834
SalI G'TCGA_C
Cuts at: 1936 2644 1936
Size: 708 4594
SanDI GG'GwC_CC
Cuts at: 2297 2297
Size: 5302
SbfI CC_TGCA'GG
Cuts at: 2654 2654
Size: 5302
ScaI AGT'ACT
Cuts at: 3550 5262 3550
Size: 1712 3590
SfcI C'TryA_G
Cuts at: 826 1045 2650 3785 4466 4657 826
Size: 219 1605 1135 681 191 1471
Fragments arranged by size:
1605 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5302
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5302
SmaI CCC'GGG
Cuts at: 1872 2629 1872
Size: 757 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3406 4307 4569 820
Size: 197 926 1463 901 262 1553
Fragments arranged by size:
1553 1463 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3093 493
Size: 2600 2702
SpeI A'CTAG_T
Cuts at: 152 2479 152
Size: 2327 2975
SphI G_CATG'C
Cuts at: 1132 2660 1132
Size: 1528 3774
SspI AAT'ATT
Cuts at: 5 52 2585 3226 5
Size: 47 2533 641 2081
Fragments arranged by size:
2533 2081 641 47
StuI AGG'CCT
Cuts at: 1517 2489 1517
Size: 972 4330
StyI C'CwwG_G
Cuts at: 513 1065 2741 513
Size: 552 1676 3074
Fragments arranged by size:
3074 1676 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3329 3346 3499 3684 4129 325
Size: 1255 1749 17 153 185 445 1498
Fragments arranged by size:
1749 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3548 5260 96
Size: 274 80 33 51 1443 1571 1712 138
Fragments arranged by size:
1712 1571 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5262 5294 5262
Size: 32 5270
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5302
XbaI T'CTAG_A
Cuts at: 2638 2638
Size: 5302
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5302
XmaI C'CCGG_G
Cuts at: 1870 2627 1870
Size: 757 4545
XmnI GAAnn'nnTTC
Cuts at: 3431 3431
Size: 5302
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3110 276
Size: 53 83 186 936 1576 2468
Fragments arranged by size:
2468 1576 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI RsrII SacI SacII SapI SexAI SfiI SfoI
SrfI SwaI XcmI