Restriction map for
pFN21AB3440
(Circular) (Six-base) MAPSORT of: pFN21AB3440.seq Check: 5293 from: 1 to: 5479
pFN21AB3440 5479 bp
With 182 enzymes: SgfI *
October 20, 2009 10:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2609 2609
Size: 5479
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3289 278
Size: 53 83 186 936 1753 2468
Fragments arranged by size:
2468 1753 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2595 2822 1937
Size: 658 227 4594
Fragments arranged by size:
4594 658 227
Acc65I G'GTAC_C
Cuts at: 2800 2800
Size: 5479
AclI AA'CG_TT
Cuts at: 1563 3606 3979 1563
Size: 2043 373 3063
Fragments arranged by size:
3063 2043 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3542 4926 5194 1632
Size: 1910 1384 268 1917
Fragments arranged by size:
1917 1910 1384 268
AfeI AGC'GCT
Cuts at: 2159 2159
Size: 5479
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5282
AflIII A'CryG_T
Cuts at: 1203 2139 4378 5355 1203
Size: 936 2239 977 1327
Fragments arranged by size:
2239 1327 977 936
AgeI A'CCGG_T
Cuts at: 4310 4310
Size: 5479
AhdI GACnn_n'nnGTC
Cuts at: 4208 4208
Size: 5479
AleI CACnn'nnGTG
Cuts at: 1166 2078 1166
Size: 912 4567
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2040 2756 1184
Size: 856 716 3907
Fragments arranged by size:
3907 856 716
AlwNI CAG_nnn'CTG
Cuts at: 2557 3222 4794 2557
Size: 665 1572 3242
Fragments arranged by size:
3242 1572 665
ApaI G_GGCC'C
Cuts at: 2349 2409 2349
Size: 60 5419
ApaLI G'TGCA_C
Cuts at: 3536 4692 3536
Size: 1156 4323
ApoI r'AATT_y
Cuts at: 1493 2788 3014 3166 5275 1493
Size: 1295 226 152 2109 1697
Fragments arranged by size:
2109 1697 1295 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4033 160
Size: 872 3001 1606
Fragments arranged by size:
3001 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5479
AvaI C'yCGr_G
Cuts at: 1870 1943 2804 1870
Size: 73 861 4545
Fragments arranged by size:
4545 861 73
AvrII C'CTAG_G
Cuts at: 2375 2375
Size: 5479
BamHI G'GATC_C
Cuts at: 2809 3176 2809
Size: 367 5112
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2800 4255 618
Size: 325 217 627 1013 1455 1842
Fragments arranged by size:
1842 1455 1013 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2349 2409 2715 2798 1391
Size: 958 60 306 83 4072
Fragments arranged by size:
4072 958 306 83 60
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4508
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3670 3704 1903
Size: 34 1733 34 3678
Fragments arranged by size:
3678 1733 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3371 4581 3371
Size: 1210 4269
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5479
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4090 136
Size: 107 122 71 631 605 2418 1525
Fragments arranged by size:
2418 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5474 5474
Size: 5479
BlpI GC'TnA_GC
Cuts at: 2896 2896
Size: 5479
Bme1580I G_kGCm'C
Cuts at: 1790 2349 2409 3540 4696 1790
Size: 559 60 1131 1156 2573
Fragments arranged by size:
2573 1156 1131 559 60
BmgBI CAC'GTC
Cuts at: 1318 2215 1318
Size: 897 4582
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3210 4168 442
Size: 416 53 83 399 24 1793 958 1753
Fragments arranged by size:
1793 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5479
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2085 2186 2309 4139 1239
Size: 846 101 123 1830 2579
Fragments arranged by size:
2579 1830 846 123 101
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3604 4469 4767 3604
Size: 865 298 4316
Fragments arranged by size:
4316 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4142 882
Size: 617 2643 2219
Fragments arranged by size:
2643 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2440 3270 493
Size: 711 1236 830 2702
Fragments arranged by size:
2702 1236 830 711
BsaBI GATnn'nnATC
Cuts at: 5473 5473
Size: 5479
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3286 3668 4303 275
Size: 53 83 186 936 1753 382 635 1451
Fragments arranged by size:
1753 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3912 4310 4584 4731 5142 3912
Size: 398 274 147 411 4249
Fragments arranged by size:
4249 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5254
Fragments arranged by size:
5254 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2204 2273 2306 2764 1186
Size: 1018 69 33 458 3901
Fragments arranged by size:
3901 1018 458 69 33
BseYI C'CCAG_C
Cuts at: 2242 2338 2504 2580 2693 2707 4682 5178 2242
Size: 96 166 76 113 14 1975 496 2543
Fragments arranged by size:
2543 1975 496 166 113 96 76 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2341 1163
Size: 573 605 4301
Fragments arranged by size:
4301 605 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2929 3690 4718 1540
Size: 275 186 928 761 1028 2301
Fragments arranged by size:
2301 1028 928 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2616 3540 3625 4696 2616
Size: 924 85 1071 3399
Fragments arranged by size:
3399 1071 924 85
BsmI GAATG_Cn'
Cuts at: 2138 2463 2990 3083 2138
Size: 325 527 93 4534
Fragments arranged by size:
4534 527 325 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2349 2409 2616 2715 2798 3540 3625
Size: 399 559 60 207 99 83 742 85
Cuts at: 3625 4696 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 559 399 207 99 85
83 60
BspEI T'CCGG_A
Cuts at: 5142 5142
Size: 5479
BspHI T'CATG_A
Cuts at: 3368 5098 3368
Size: 1730 3749
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2008 2433 2609 2834 844
Size: 1164 425 176 225 3489
Fragments arranged by size:
3489 1164 425 225 176
BsrBI CCG'CTC
Cuts at: 1394 1823 3366 1394
Size: 429 1543 3507
Fragments arranged by size:
3507 1543 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3974 4148 65
Size: 1183 2726 174 1396
Fragments arranged by size:
2726 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4123 4310 1700
Size: 110 2313 187 2869
Fragments arranged by size:
2869 2313 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5479
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5479
BssSI C'ACGA_G
Cuts at: 3539 4551 3539
Size: 1012 4467
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5479
BstBI TT'CG_AA
Cuts at: 4283 4283
Size: 5479
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5479
BstYI r'GATC_y
Cuts at: 1972 2553 2771 2809 3176 3561 3578 5030 5116
Size: 581 218 38 367 385 17 1452 86
Cuts at: 5116 5128 5474 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 581 385 367 346 218 86
38 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2640 513
Size: 552 1575 3352
Fragments arranged by size:
3352 1575 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4740
Fragments arranged by size:
4740 683 56
BtsI GCAGTG_nn'
Cuts at: 2310 2995 3789 3809 2310
Size: 685 794 20 3980
Fragments arranged by size:
3980 794 685 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5479
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5444
DraI TTT'AAA
Cuts at: 2783 2783
Size: 5479
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4486 809
Size: 507 3170 1802
Fragments arranged by size:
3170 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2926 3815 5444
Size: 54 1425 50 275 18 1096 889 1629
Cuts at: 5444 8
Size: 43
Fragments arranged by size:
1629 1425 1096 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2926 1537
Size: 275 1114 4090
Fragments arranged by size:
4090 1114 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3409 1353
Size: 2056 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4066 4440 4586 387
Size: 1064 138 2477 374 146 1280
Fragments arranged by size:
2477 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2085 2186 2309 3542 4139 4926 5194
Size: 393 453 101 123 1233 597 787 268
Cuts at: 5194 1239
Size: 1524
Fragments arranged by size:
1524 1233 787 597 453 393 268 123
101
EcoNI CCTnn'n_nnAGG
Cuts at: 2117 2117
Size: 5479
EcoO109I rG'GnC_Cy
Cuts at: 2405 2406 2405
Size: 1 5478
EcoRI G'AATT_C
Cuts at: 2788 3166 2788
Size: 378 5101
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2425 2425
Size: 5479
FspI TGC'GCA
Cuts at: 3985 3985
Size: 5479
HaeII r_GCGC'y
Cuts at: 1678 2161 2365 4626 1678
Size: 483 204 2261 2531
Fragments arranged by size:
2531 2261 483 204
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2029 2061 2718 2750 4120
Size: 32 135 32 457 32 657 32 1370
Cuts at: 4120 4152 4194 4226 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1370 657 457 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2823 3069 669
Size: 988 281 885 246 3079
Fragments arranged by size:
3079 988 885 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5479
HpaI GTT'AAC
Cuts at: 3069 3069
Size: 5479
KpnI G_GTAC'C
Cuts at: 2804 2804
Size: 5479
MfeI C'AATT_G
Cuts at: 3078 3078
Size: 5479
MluI A'CGCG_T
Cuts at: 2139 5355 2139
Size: 3216 2263
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4593 4777 1305
Size: 3288 184 2007
Fragments arranged by size:
3288 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3657
Fragments arranged by size:
3657 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2078 2738 3437 3796 3955 518
Size: 648 912 660 699 359 159 2042
Fragments arranged by size:
2042 912 699 660 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2504 2895 3572 4720 4965 1606
Size: 898 391 677 1148 245 2120
Fragments arranged by size:
2120 1148 898 677 391 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4927
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5479
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5479
NotI GC'GGCC_GC
Cuts at: 2926 2926
Size: 5479
NspI r_CATG'y
Cuts at: 1132 1229 2837 4382 1132
Size: 97 1608 1545 2229
Fragments arranged by size:
2229 1608 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5479
PciI A'CATG_T
Cuts at: 4378 4378
Size: 5479
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2557 1108
Size: 1449 4030
PfoI T'CCnGG_A
Cuts at: 2163 2286 2163
Size: 123 5356
PmeI GTTT'AAAC
Cuts at: 2783 2783
Size: 5479
PmlI CAC'GTG
Cuts at: 2440 2440
Size: 5479
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3548 3580 5061 5093 3548
Size: 32 1481 32 3934
Fragments arranged by size:
3934 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5479
PsiI TTA'TAA
Cuts at: 3049 3049
Size: 5479
PspOMI G'GGCC_C
Cuts at: 2345 2405 2345
Size: 60 5419
PstI C_TGCA'G
Cuts at: 830 2019 2361 2779 2831 830
Size: 1189 342 418 52 3478
Fragments arranged by size:
3478 1189 418 342 52
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5479
PvuII CAG'CTG
Cuts at: 2504 2504
Size: 5479
SalI G'TCGA_C
Cuts at: 1936 2821 1936
Size: 885 4594
SbfI CC_TGCA'GG
Cuts at: 2831 2831
Size: 5479
ScaI AGT'ACT
Cuts at: 2264 3727 5439 2264
Size: 1463 1712 2304
Fragments arranged by size:
2304 1712 1463
SfcI C'TryA_G
Cuts at: 826 1045 2015 2357 2775 2827 3962 4643 4834
Size: 219 970 342 418 52 1135 681 191
Cuts at: 4834 826
Size: 1471
Fragments arranged by size:
1471 1135 970 681 418 342 219 191
52
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5479
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5479
SmaI CCC'GGG
Cuts at: 1872 2806 1872
Size: 934 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3583 4484 4746 820
Size: 197 926 1640 901 262 1553
Fragments arranged by size:
1640 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3270 493
Size: 2777 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5479
SphI G_CATG'C
Cuts at: 1132 2837 1132
Size: 1705 3774
SspI AAT'ATT
Cuts at: 5 52 3403 5
Size: 47 3351 2081
Fragments arranged by size:
3351 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5479
StyI C'CwwG_G
Cuts at: 513 1065 2375 2918 513
Size: 552 1310 543 3074
Fragments arranged by size:
3074 1310 552 543
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3506 3523 3676 3861 4306 325
Size: 1255 1926 17 153 185 445 1498
Fragments arranged by size:
1926 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2262 3725 5437
Size: 274 80 33 51 1443 285 1463 1712
Cuts at: 5437 96
Size: 138
Fragments arranged by size:
1712 1463 1443 285 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2145 2177 2303 2335 5439 5471 2145
Size: 32 126 32 3104 32 2153
Fragments arranged by size:
3104 2153 126 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5479
XbaI T'CTAG_A
Cuts at: 2815 2815
Size: 5479
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5479
XmaI C'CCGG_G
Cuts at: 1870 2804 1870
Size: 934 4545
XmnI GAAnn'nnTTC
Cuts at: 3608 3608
Size: 5479
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3287 276
Size: 53 83 186 936 1753 2468
Fragments arranged by size:
2468 1753 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PpuMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI