Restriction map for
pFN21AB3435
(Circular) (Six-base) MAPSORT of: pFN21AB3435.seq Check: 1370 from: 1 to: 5923
pFN21AB3435 5923 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3733 278
Size: 53 83 186 936 2197 2468
Fragments arranged by size:
2468 2197 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3266 1937
Size: 1329 4594
Acc65I G'GTAC_C
Cuts at: 2474 3244 2474
Size: 770 5153
AclI AA'CG_TT
Cuts at: 1563 4050 4423 1563
Size: 2487 373 3063
Fragments arranged by size:
3063 2487 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3986 5370 5638 1632
Size: 2354 1384 268 1917
Fragments arranged by size:
2354 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5726
AflIII A'CryG_T
Cuts at: 1203 4822 5799 1203
Size: 3619 977 1327
Fragments arranged by size:
3619 1327 977
AgeI A'CCGG_T
Cuts at: 4754 4754
Size: 5923
AhdI GACnn_n'nnGTC
Cuts at: 4652 4652
Size: 5923
AleI CACnn'nnGTG
Cuts at: 1166 2420 1166
Size: 1254 4669
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2438 3032 1184
Size: 1254 594 4075
Fragments arranged by size:
4075 1254 594
AlwNI CAG_nnn'CTG
Cuts at: 2443 3666 5238 2443
Size: 1223 1572 3128
Fragments arranged by size:
3128 1572 1223
ApaLI G'TGCA_C
Cuts at: 3980 5136 3980
Size: 1156 4767
ApoI r'AATT_y
Cuts at: 1493 3232 3458 3610 5719 1493
Size: 1739 226 152 2109 1697
Fragments arranged by size:
2109 1739 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4477 160
Size: 872 3445 1606
Fragments arranged by size:
3445 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5923
AvaI C'yCGr_G
Cuts at: 1870 1943 2331 2886 3248 1870
Size: 73 388 555 362 4545
Fragments arranged by size:
4545 555 388 362 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2389 2422 2389
Size: 33 5890
BamHI G'GATC_C
Cuts at: 3253 3620 3253
Size: 367 5556
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2387 2414 2474 3244 4699
Size: 325 217 627 600 27 60 770 1455
Cuts at: 4699 618
Size: 1842
Fragments arranged by size:
1842 1455 770 627 600 325 217 60
27
BanII G_rGCy'C
Cuts at: 1391 2061 3242 1391
Size: 670 1181 4072
Fragments arranged by size:
4072 1181 670
BbeI G_GCGC'C
Cuts at: 2391 2391
Size: 5923
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3147 928
Size: 971 1248 3704
Fragments arranged by size:
3704 1248 971
BbvCI CC'TCA_GC
Cuts at: 2430 2430
Size: 5923
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4114 4148 1903
Size: 34 2177 34 3678
Fragments arranged by size:
3678 2177 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2750 2911 3815 5025 2750
Size: 161 904 1210 3648
Fragments arranged by size:
3648 1210 904 161
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5923
BfrBI ATG'CAT
Cuts at: 2881 2911 2881
Size: 30 5893
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2309 4534 136
Size: 107 122 71 631 605 637 2225 1525
Fragments arranged by size:
2225 1525 637 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2293 2589 5918 2293
Size: 296 3329 2298
Fragments arranged by size:
3329 2298 296
BlpI GC'TnA_GC
Cuts at: 2343 3340 2343
Size: 997 4926
Bme1580I G_kGCm'C
Cuts at: 1790 2417 3984 5140 1790
Size: 627 1567 1156 2573
Fragments arranged by size:
2573 1567 1156 627
BmgBI CAC'GTC
Cuts at: 1318 2941 1318
Size: 1623 4300
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2253 3654 4612
Size: 416 53 83 399 24 836 1401 958
Cuts at: 4612 442
Size: 1753
Fragments arranged by size:
1753 1401 958 836 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5923
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2185 2185
Size: 5923
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2753 4583 1239
Size: 1514 1830 2579
Fragments arranged by size:
2579 1830 1514
Bpu10I CC'TnA_GC
Cuts at: 2430 2430
Size: 5923
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2211 2687 4048 4913 5211 2211
Size: 476 1361 865 298 2923
Fragments arranged by size:
2923 1361 865 476 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3161 4586 882
Size: 617 1662 1425 2219
Fragments arranged by size:
2219 1662 1425 617
BsaAI yAC'GTr
Cuts at: 493 1204 3714 493
Size: 711 2510 2702
Fragments arranged by size:
2702 2510 711
BsaBI GATnn'nnATC
Cuts at: 5917 5917
Size: 5923
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2067 2388 3730 4112
Size: 53 83 186 936 534 321 1342 382
Cuts at: 4112 4747 275
Size: 635 1451
Fragments arranged by size:
1451 1342 936 635 534 382 321 186
83 53
BsaWI w'CCGG_w
Cuts at: 2505 4356 4754 5028 5175 5586 2505
Size: 1851 398 274 147 411 2842
Fragments arranged by size:
2842 1851 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3167 3197 1178
Size: 30 165 30 1764 30 3904
Fragments arranged by size:
3904 1764 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2786 1186
Size: 1600 4323
BseYI C'CCAG_C
Cuts at: 5126 5622 5126
Size: 496 5427
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2456 3038 1163
Size: 573 720 582 4048
Fragments arranged by size:
4048 720 582 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3088 3373 4134 5162 1540
Size: 275 186 1087 285 761 1028 2301
Fragments arranged by size:
2301 1087 1028 761 285 275 186
BsiHKAI G_wGCw'C
Cuts at: 3984 4069 5140 3984
Size: 85 1071 4767
Fragments arranged by size:
4767 1071 85
BsmI GAATG_Cn'
Cuts at: 3434 3527 3434
Size: 93 5830
BsmBI CGTCTCn'nnnn_
Cuts at: 2931 2931
Size: 5923
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2061 2417 3242 3984 4069 5140 1391
Size: 399 271 356 825 742 85 1071 2174
Fragments arranged by size:
2174 1071 825 742 399 356 271 85
BspEI T'CCGG_A
Cuts at: 5586 5586
Size: 5923
BspHI T'CATG_A
Cuts at: 2685 3812 5542 2685
Size: 1127 1730 3066
Fragments arranged by size:
3066 1730 1127
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3003 3013 3278 844
Size: 2159 10 265 3489
Fragments arranged by size:
3489 2159 265 10
BsrBI CCG'CTC
Cuts at: 1394 1823 3810 1394
Size: 429 1987 3507
Fragments arranged by size:
3507 1987 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4418 4592 65
Size: 1183 3170 174 1396
Fragments arranged by size:
3170 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4567 4754 1700
Size: 110 2757 187 2869
Fragments arranged by size:
2869 2757 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5923
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5923
BssSI C'ACGA_G
Cuts at: 3983 4995 3983
Size: 1012 4911
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2443 794
Size: 1649 4274
BstBI TT'CG_AA
Cuts at: 4727 4727
Size: 5923
BstEII G'GTnAC_C
Cuts at: 1184 2671 1184
Size: 1487 4436
BstYI r'GATC_y
Cuts at: 1972 2293 2589 3253 3620 4005 4022 5474 5560
Size: 321 296 664 367 385 17 1452 86
Cuts at: 5560 5572 5918 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 664 385 367 346 321 296
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2137 2572 513
Size: 552 1072 435 3864
Fragments arranged by size:
3864 1072 552 435
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5184
Fragments arranged by size:
5184 683 56
BtsI GCAGTG_nn'
Cuts at: 3439 4233 4253 3439
Size: 794 20 5109
Fragments arranged by size:
5109 794 20
ClaI AT'CG_AT
Cuts at: 1554 2270 1554
Size: 716 5207
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5888
DraI TTT'AAA
Cuts at: 3227 3227
Size: 5923
DraIII CAC_nnn'GTG
Cuts at: 2795 2795
Size: 5923
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4930 809
Size: 507 3614 1802
Fragments arranged by size:
3614 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2253 2482 3000
Size: 54 1425 50 275 18 423 229 518
Cuts at: 3000 3103 3370 4259 5888 8
Size: 103 267 889 1629 43
Fragments arranged by size:
1629 1425 889 518 423 275 267 229
103 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3370 1537
Size: 275 1558 4090
Fragments arranged by size:
4090 1558 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2715 3159 3853 1353
Size: 1362 444 694 3423
Fragments arranged by size:
3423 1362 694 444
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2732 2893 4510 4884 5030 387
Size: 1064 138 1143 161 1617 374 146 1280
Fragments arranged by size:
1617 1280 1143 1064 374 161 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2753 3986 4583 5370 5638 1239
Size: 393 1121 1233 597 787 268 1524
Fragments arranged by size:
1524 1233 1121 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2692 2692
Size: 5923
EcoRI G'AATT_C
Cuts at: 3232 3610 3232
Size: 378 5545
FspI TGC'GCA
Cuts at: 4429 4429
Size: 5923
HaeII r_GCGC'y
Cuts at: 1678 2391 5070 1678
Size: 713 2679 2531
Fragments arranged by size:
2679 2531 713
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2012 2044 2440 2472 2869
Size: 32 135 32 440 32 396 32 397
Cuts at: 2869 2901 4564 4596 4638 4670 1373
Size: 32 1663 32 42 32 2626
Fragments arranged by size:
2626 1663 440 397 396 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2098 2727 3267 3513 669
Size: 988 281 160 629 540 246 3079
Fragments arranged by size:
3079 988 629 540 281 246 160
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5923
HpaI GTT'AAC
Cuts at: 3513 3513
Size: 5923
KasI G'GCGC_C
Cuts at: 2387 2387
Size: 5923
KpnI G_GTAC'C
Cuts at: 2478 3248 2478
Size: 770 5153
MfeI C'AATT_G
Cuts at: 3522 3522
Size: 5923
MluI A'CGCG_T
Cuts at: 5799 5799
Size: 5923
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5037 5221 1305
Size: 3732 184 2007
Fragments arranged by size:
3732 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3002 10
Size: 54 1425 343 1170 2931
Fragments arranged by size:
2931 1425 1170 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2420 2468 2534 2759 3014 3881 4240
Size: 648 1254 48 66 225 255 867 359
Cuts at: 4240 4399 518
Size: 159 2042
Fragments arranged by size:
2042 1254 867 648 359 255 225 159
66 48
MspA1I CmG'CkG
Cuts at: 1606 3339 4016 5164 5409 1606
Size: 1733 677 1148 245 2120
Fragments arranged by size:
2120 1733 1148 677 245
NarI GG'CG_CC
Cuts at: 2388 2388
Size: 5923
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5371
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5923
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5923
NotI GC'GGCC_GC
Cuts at: 3370 3370
Size: 5923
NsiI A_TGCA'T
Cuts at: 2883 2913 2883
Size: 30 5893
NspI r_CATG'y
Cuts at: 1132 1229 2885 3281 4826 1132
Size: 97 1656 396 1545 2229
Fragments arranged by size:
2229 1656 1545 396 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5923
PciI A'CATG_T
Cuts at: 4822 4822
Size: 5923
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2314 1108
Size: 1206 4717
PmeI GTTT'AAAC
Cuts at: 3227 3227
Size: 5923
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3992 4024 5505 5537 3992
Size: 32 1481 32 4378
Fragments arranged by size:
4378 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2692 2692
Size: 5923
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5923
PsiI TTA'TAA
Cuts at: 3493 3493
Size: 5923
PstI C_TGCA'G
Cuts at: 830 2280 2448 3275 830
Size: 1450 168 827 3478
Fragments arranged by size:
3478 1450 827 168
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5923
SalI G'TCGA_C
Cuts at: 1936 3265 1936
Size: 1329 4594
SanDI GG'GwC_CC
Cuts at: 2692 2692
Size: 5923
SbfI CC_TGCA'GG
Cuts at: 3275 3275
Size: 5923
ScaI AGT'ACT
Cuts at: 4171 5883 4171
Size: 1712 4211
SfcI C'TryA_G
Cuts at: 826 1045 2276 2444 3271 4406 5087 5278 826
Size: 219 1231 168 827 1135 681 191 1471
Fragments arranged by size:
1471 1231 1135 827 681 219 191 168
SfoI GGC'GCC
Cuts at: 2389 2389
Size: 5923
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5923
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5923
SmaI CCC'GGG
Cuts at: 1872 3250 1872
Size: 1378 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2226 2331 2666 2886 4027 4928
Size: 197 926 283 105 335 220 1141 901
Cuts at: 4928 5190 820
Size: 262 1553
Fragments arranged by size:
1553 1141 926 901 335 283 262 220
197 105
SnaBI TAC'GTA
Cuts at: 493 3714 493
Size: 3221 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5923
SphI G_CATG'C
Cuts at: 1132 2885 3281 1132
Size: 1753 396 3774
Fragments arranged by size:
3774 1753 396
SspI AAT'ATT
Cuts at: 5 52 3847 5
Size: 47 3795 2081
Fragments arranged by size:
3795 2081 47
StuI AGG'CCT
Cuts at: 1517 2312 1517
Size: 795 5128
StyI C'CwwG_G
Cuts at: 513 1065 2313 3362 513
Size: 552 1248 1049 3074
Fragments arranged by size:
3074 1248 1049 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2321 2341 2639 2837 3074 3950 3967
Size: 1255 741 20 298 198 237 876 17
Cuts at: 3967 4120 4305 4750 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 876 741 445 298 237 198
185 153 20 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2542 4169 5881
Size: 274 80 33 51 1443 565 1627 1712
Cuts at: 5881 96
Size: 138
Fragments arranged by size:
1712 1627 1443 565 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5883 5915 5883
Size: 32 5891
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5923
XbaI T'CTAG_A
Cuts at: 3259 3259
Size: 5923
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2240 2311 2335 2995 2240
Size: 71 24 660 5168
Fragments arranged by size:
5168 660 71 24
XhoI C'TCGA_G
Cuts at: 1943 2331 2886 1943
Size: 388 555 4980
Fragments arranged by size:
4980 555 388
XmaI C'CCGG_G
Cuts at: 1870 3248 1870
Size: 1378 4545
XmnI GAAnn'nnTTC
Cuts at: 4052 4052
Size: 5923
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3731 276
Size: 53 83 186 936 2197 2468
Fragments arranged by size:
2468 2197 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BsiWI BstXI
BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI
NaeI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SapI SexAI SfiI
SrfI SwaI